LOCUS       NC_017314               5143 bp    DNA     circular CON 08-JUL-2023
DEFINITION  Enterococcus faecalis 62 plasmid EF62pA, complete sequence.
ACCESSION   NC_017314
VERSION     NC_017314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603509
            Assembly: GCF_000211255.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis 62
  ORGANISM  Enterococcus faecalis 62
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Brede,D.A., Snipen,L.G., Ussery,D.W., Nederbragt,A.J. and Nes,I.F.
  TITLE     Complete genome sequence of the commensal Enterococcus faecalis 62,
            isolated from a healthy Norwegian infant
  JOURNAL   J Bacteriol 193 (9), 2377-2378 (2011)
   PUBMED   21398545
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Solheim,M., Aakra,A., Snipen,L.G., Brede,D.A. and Nes,I.F.
  TITLE     Comparative genomics of Enterococcus faecalis from healthy Norwegian
            infants
  JOURNAL   BMC Genomics 10, 194 (2009)
   PUBMED   19393078
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5143)
  AUTHORS   Brede,D.A., Snipen,L.G., Ussery,D.W., Nederbragt,A.J. and Nes,I.F.
  TITLE     Complete genome sequence of the commensal Enterococcus faecalis 62
            isolated from a healthy Norwegian infant
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 5143)
  AUTHORS   Brede,D.A., Snipen,L.G., Ussery,D.W., Nederbragt,A.J. and Nes,I.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2010) Department of Chemistry, Biotechnology and
            Food Science, Norwegian University of Life Sciences, Postboks 5003,
            Aas N-1432, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/08/2023 01:18:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,045
            CDSs (total)                      :: 2,975
            Genes (coding)                    :: 2,886
            CDSs (with protein)               :: 2,886
            Genes (RNA)                       :: 70
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 40 of 89
            Pseudo Genes (incomplete)         :: 34 of 89
            Pseudo Genes (internal stop)      :: 32 of 89
            Pseudo Genes (multiple problems)  :: 16 of 89
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP002492.1.
            DNA source: Dag Anders Brede (dag.anders.brede@umb.no) and Ingolf
            F. Nes (ingolf.nes@umb.no).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Enterococcus faecalis 62"
                     /mol_type="genomic DNA"
                     /strain="62"
                     /db_xref="taxon:936153"
                     /plasmid="EF62pA"
     gene            86..286
                     /locus_tag="EF62_RS15395"
                     /old_locus_tag="EF62_pA0001"
     CDS             86..286
                     /locus_tag="EF62_RS15395"
                     /old_locus_tag="EF62_pA0001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     gene            complement(805..891)
                     /locus_tag="EF62_RS15405"
     CDS             complement(805..891)
                     /locus_tag="EF62_RS15405"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            1538..2260
                     /locus_tag="EF62_RS15410"
                     /old_locus_tag="EF62_pA0004"
     CDS             1538..2260
                     /locus_tag="EF62_RS15410"
                     /old_locus_tag="EF62_pA0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002360724.1"
                     /translation="MSDKSELAVKYQNELNLVPLKNFNAKQMDLFFALCARMKDKGVEN
                     IRFSFEELKELSDYKMTATKAFVADLEKLYKDMLNLSYRTENDDEIEYFVLFNGFKIDK
                     KQKFVEVRVNQDLDYIINGLTTEFSRFELSAFTSIRSTYAKTLFRLLMQYRSTGYYVVS
                     IEDFRELLDIPEYYQMGNIDQKVLKPAMKELHNYFENLEVTKIKAKKGNKIAKLEFTFT
                     GLKTNKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            2387..2683
                     /locus_tag="EF62_RS15415"
                     /old_locus_tag="EF62_pA0005"
     CDS             2387..2683
                     /locus_tag="EF62_RS15415"
                     /old_locus_tag="EF62_pA0005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002360723.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLERLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLIDTVAYKVTLEEDISCGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            3461..5140
                     /gene="mobV"
                     /locus_tag="EF62_RS15425"
                     /old_locus_tag="EF62_pA0008"
     CDS             3461..5140
                     /gene="mobV"
                     /locus_tag="EF62_RS15425"
                     /old_locus_tag="EF62_pA0008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_010717414.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     Typing          complement(2916..3175)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="81.0"
                     /evalue="1.95e-45"
     Typing          1539..2260
                     /name="rep_cluster_2301"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3387..5137
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 taatttaatt gataaattat tgacacataa aaacagttaa attaaaatat attaataaaa
       61 attaaaaaaa agtgaggtaa gcattatgaa aaagttgtta gttctaattt taggggtgtc
      121 ttttatctct gttgctttaa gttcaactac agaagcaagt gctagagagt gggattctat
      181 agagtgtcag ccaggatttt attggaaaaa aggcgggtgg actgcaggag gatatcataa
      241 agccggtgga ttttgtatcc caatgcatac aagatggaca aaatagttgc taaatttgag
      301 gttatttcat gaaaaaatat ctaataaaaa atatatttta tattactatt cctttagttt
      361 taagctatat aacttctttt ttagttaata ttgatttgcc catattgata attatttttt
      421 acggaatttt attatttttt ttaattcctt cagaagtata tttgggaagt acaatggatt
      481 acaacgcgaa agtagtgaat cccacttaca gaccagaaaa taaaagcttt gaagatagtc
      541 caaaaagtaa aatattgtct atcctcatag tattactatg tttaatacta actatttcga
      601 tatggtattt ctctaattga cgttttgcgt aatctaatct gatgtttgaa aatacagaaa
      661 aaaaatgtta ttgtgtaagt gcgaagcgaa attaagcaaa atcatgttta caccaaaaag
      721 ccctcctatt ctcagtagga gggctttttt acactgttgc gatttcagcg ttggagctag
      781 aaaagcaact gcatagatta tctgctaatc gtctagccaa cgatcaacta gcaaaagtac
      841 taaacccaca aaaagtgggg cgataatcaa cgaaattaag ctttcaatca tgtttacacc
      901 ccctttcggg gcagttgcca aaacaagtat atcacaagaa gaatttcttg tgggggttgt
      961 tagggcttgc cctgaccgcc tgcaaggcgc aatcctaacc aagataccct aaaacaacta
     1021 aacatgtctt aaagatcttc agacaagcaa atagagccta accaaatgat ttaccttgtc
     1081 aacggtattg attttgattt tagcgagtga acgaagtgaa cgctgcaagc aaaatgtgag
     1141 cgggattttc gctcactcct tttttgattt tttagtgcca gttttgtaaa tatcgcggac
     1201 ctgtttgtag aaaaaagagg ttaattttgg gcgggaaatt tccgcgcgcg cacgcgtttc
     1261 ttttatatat attttatata tatttttaaa acctttttag gcgggctgtg tgccacgtgg
     1321 ctctaaggaa gagcagatgg taccaacaac tttttatctg actaccaaca actttttatc
     1381 tgactaccaa caacttttta tctgactacc aacaactttt tatctgacta ccaacaactt
     1441 tttatctgtt ttattttcat aatattaaaa caaacaacca actgttgaca aagttggttg
     1501 tttgttttaa tattcgaatt gtgagggggc gaaaggaatg agtgataaat cggaattagc
     1561 tgttaaatat caaaatgagt taaatcttgt gccacttaaa aactttaatg caaaacaaat
     1621 ggatctgttc tttgcgttat gcgcacgaat gaaagataaa ggagtagaga atattcgttt
     1681 ctccttcgag gaattaaagg aattgagtga ctacaaaatg actgctacaa aagcctttgt
     1741 agcagactta gaaaagttgt ataaagatat gctgaacctt tcttatcgta cagaaaacga
     1801 tgacgaaata gagtattttg ttttattcaa tggatttaaa atcgacaaaa aacaaaagtt
     1861 tgttgaagtt cgagttaatc aagatttaga ctatataata aatggtttaa caacagagtt
     1921 tagtaggttt gagctgtccg cttttacttc aatacgctca acatatgcta aaacattgtt
     1981 tcgtttgtta atgcaatatc gttcaacagg gtattatgta gtcagtattg aagacttcag
     2041 agaattgtta gacatacccg aatattatca aatgggtaac attgaccaaa aggttttaaa
     2101 accagcaatg aaagaactcc ataactattt tgagaattta gaagtaacaa aaatcaaagc
     2161 caaaaaaggc aataagattg ccaaactaga atttacgttt actggtctga aaaccaacaa
     2221 accaagcgta accatgcacg attgggtaaa cggagaataa aaaaagaact tgccccctca
     2281 cattaagcaa gttagcaagt tcctcacgca atcttccgga ctgctagatt attttagcat
     2341 atttgcgtga ggacttctaa aataaattat tttggaggtc ctacttatgg gattaatgac
     2401 aggatacaac atgacaaaag aggagtacga agtcacacag gctgtactgg agcgactaga
     2461 taagtttgag agtgaactag aagcgctatt gcttgatttg gagccacttg acttatcacg
     2521 attagatact gtagttcgag acgaaacgct aagaatgttt aattatctaa gtcggtcata
     2581 cattctactt attgatacgg tagcctacaa agttacgcta gaagaagata tttcctgtgg
     2641 gattgtgaag attgacggtc aatctctaga cgaagaagaa taaaaactac ccttgcttgt
     2701 tataaaaaaa tgttgactat ttgggataac actcgaaaaa tatcaagatt agcctacttc
     2761 cattttgggg taggctttct ttttgcaagc ggagcaacat ggtaccatgt tgcgatattt
     2821 tgaacgactc ctaccaaaaa tactggtagg agtcgttcaa atttttgggg ttcccccaat
     2881 cccactgctg ttattttttg cttttactgt tgaaattcgc ttaatttctt cctgtgtttc
     2941 ggaagagcat aaggggaact ggggggcaca caaggtaccc cgccttgcag tgcaaggctt
     3001 tcccgtgggg tacactttcg ttcttcgcta gcactttact agcaggtact ttcaggtaaa
     3061 ctagcacatt tttagcactc aatagcaggt ttctagcacc caaactagca gattcctagc
     3121 agtgactagc aggtttttag caccaaatta gcacattact agcaccggct agcagaaagc
     3181 tagcacgttc actaatttca ggcagtcgtt atttaaacgg ttttctgacg gagggaaatt
     3241 tggagtttct gccttagcag aaatggaaag tttgaccgta ggcaatcggc taagtgcgag
     3301 attgggaagc ctaaaggctg acctttcgag tactagccga cagccctcgg cagagcgcac
     3361 ctttacgaag taaagtatag tgcgttatac tttacttgga agtactgccg aaatccaaag
     3421 acaccgaacg acacaagaaa aaaaaggggg aggagcgctc atgtcaaaaa ttgttgcacg
     3481 tatggaaaaa atgaaagacg gaaatttaag tggtatccaa cgacataatc aacgagaaac
     3541 caataatcat tccaatcctg atattgatat tgagaaatct cacttgaatt atgacttagt
     3601 caatcctggt tcaatcaatt atcgggagaa aatcaaacaa atcattgaga gccaacgaat
     3661 cagtaaacga gcggttagaa aagacgcagt ccttgtgaac gaatggataa tcactagtga
     3721 taccgccttt tttcaagaga atacagacac acaagcattt tttaccgatg ttgtcgcata
     3781 tttctctgat cgctgcggtc gacaaaatgt cgcctatgcc acggtacatt tagacgaaac
     3841 cacgccccat atgcacttag gaattgtgcc tatgtacgaa gggcgattga gcagtaaaca
     3901 ggtgtttagt cggcaaaatt tgttagagat tcaagaagaa ttgccaacct atttgcaaga
     3961 acgaggctat gctattgagc gtggactccg tgggagtccg caaaagcatt tatctgtcaa
     4021 agaatacaaa gacgttcaaa aggagctaca agtttctaag caggaactcg ctaatcagga
     4081 acaaattctg gcagaaaaag agagagaact ccattcttat gaacaggcaa tcttccgtat
     4141 tgaccgcctt cttgaagaga agaacgaaga agtaaagggc aaagtccaag caatgaacca
     4201 actggaaacg aagcgaaacg aaatcattaa ctctctacaa acaaccgttt tacctgattt
     4261 agcaaatgtc aaagaaaaga aatttagttt aaacggttta aaatacgttt taagcgcttc
     4321 tgacttttcg gcgataaaaa gtattacgaa agatttcgag acgcttagaa cgcaaaataa
     4381 gcaccttaaa atcgaaaaca ataacttggc acagaaaaat aaagaattga cacaagtaaa
     4441 tcatgaacaa gaattacaaa taacggaatt agcctatgaa ttaaccctga aaaaagaaca
     4501 attaacagcg tttgaagaaa aacaactaaa ctatgaccag agcgaacaaa tcaaacaaga
     4561 acttgataca acaacacaaa cagtttctgt gttagaaaca gagaataagc ggttaaaaga
     4621 taaaattact gacttaacct acgacttttc tgaactaaat gaaaaatatc aagaaataaa
     4681 attaaagttt gacgggttaa ctacttacct tcacgaaaga ctaggtcatg ctaaagaaac
     4741 cattttattt acgattcagc aaggagtgaa gtaccttcag aacgcgccta aattaaaaaa
     4801 atccctagag cgatattcca aggatagaac ttacctcatt gacgaaaaag ggacattgtt
     4861 tttaccacca gatacggggt caaaaggggt aacaatgtcc ttaaacccac atttctaccc
     4921 cctaaaagcc ccgttttaca acagagaaac agggtgttat gagtttgtcg gcgaatatat
     4981 ggctaatgga cgttctcgaa cgaatacttt acaactgtct gaagaacaag tgctagagaa
     5041 gaaagctttt acgtttgaac agtcgacaga taaaaaggct tggcaacaga gtaaaaaata
     5101 ttcaagagat atgtcaaaag gacgaggttt atctcgttaa aaa
//
