LOCUS       NC_016834               8953 bp    DNA     circular CON 17-APR-2024
DEFINITION  Shigella sonnei 53G plasmid E, complete sequence.
ACCESSION   NC_016834
VERSION     NC_016834.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA2272161
            Assembly: GCF_000283715.1
KEYWORDS    RefSeq; complete genome; complete replicon.
SOURCE      Shigella sonnei 53G
  ORGANISM  Shigella sonnei 53G
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1
  AUTHORS   Holt,K.E., Baker,S., Weill,F.X., Holmes,E.C., Kitchen,A., Yu,J.,
            Sangal,V., Brown,D.J., Coia,J.E., Kim,D.W., Choi,S.Y., Kim,S.H., da
            Silveira,W.D., Pickard,D.J., Farrar,J.J., Parkhill,J., Dougan,G. and
            Thomson,N.R.
  TITLE     Shigella sonnei genome sequencing and phylogenetic analysis indicate
            recent global dissemination from Europe
  JOURNAL   Nat Genet 44 (9), 1056-1059 (2012)
   PUBMED   22863732
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   Aslett,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2011) Aslett M.A., Wellcome Trust Sanger
            Institute, Pathogen Sequencing Unit, Wellcome Trust Genome Campus,
            Hinxton, Cambridge, Cambridgeshire CB10 1SA, UNITED KINGDOM
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000283715.1-RS_2024_04_17
            Annotation Date                   :: 04/17/2024 01:45:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,357
            CDSs (total)                      :: 5,227
            Genes (coding)                    :: 4,564
            CDSs (with protein)               :: 4,564
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 663
            CDSs (without protein)            :: 663
            Pseudo Genes (ambiguous residues) :: 0 of 663
            Pseudo Genes (frameshifted)       :: 184 of 663
            Pseudo Genes (incomplete)         :: 478 of 663
            Pseudo Genes (internal stop)      :: 110 of 663
            Pseudo Genes (multiple problems)  :: 104 of 663
            Pseudo Genes (short protein)      :: 3 of 663
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            HE616532.1.
            full annotation will be provided prior to release of this entry
            Accession numbers for all the replicons in this genome project:
            HE616528-HE616532.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8953
                     /organism="Shigella sonnei 53G"
                     /mol_type="genomic DNA"
                     /strain="53G"
                     /db_xref="taxon:216599"
                     /plasmid="E"
                     /note="LPS plasmid"
     gene            1..957
                     /locus_tag="SSON53G_RS27645"
     CDS             1..957
                     /locus_tag="SSON53G_RS27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293214.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293214.1"
                     /translation="MSELVVFKANELAVSRYDLTEHETKLILFCVAKLNPTIENPTRDE
                     LTVKFSCSEYARTMGLSYENAWGRLNSATSDLFKRSVELIYPTGAVSKRIFNWTEYAEF
                     NREEQTVTLVFSSYIQPLLFHLKKFIKYNLEHVKAFENKYSMRIYEWLLKELSQRKTHR
                     GNIEISIKEFKFMLMLEKNYPLYAELNRWILKPVTNDLNTYSNMKLTIEKRGRPADTLI
                     FQVELDKQIDLVTELAKDPASKKEDKTIRLTPENRLHEGLKTTLHDALTAKIQLTSFEA
                     KFLSDMQSKYDLNGSFTWLTQKQRTTLEKILAKYGRI"
                     /name="replication initiation protein"
     gene            965..1282
                     /locus_tag="SSON53G_RS27650"
     CDS             965..1282
                     /locus_tag="SSON53G_RS27650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229902.1"
                     /translation="MALVSVTEAARLTGKNRAIIHRYIKQGKLSQISDATNTKKVDTSE
                     LIRVFGSLTATHATLSQSDAMQHDATPKTDQSVALLQQKIALLEQLLESKNNELQRQDE
                     R"
                     /name="hypothetical protein"
     gene            1333..2030
                     /locus_tag="SSON53G_RS33275"
     CDS             join(1333..1582,1582..2030)
                     /locus_tag="SSON53G_RS33275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1SD family transposase"
                     /protein_id="WP_228769501.1"
                     /translation="MASVSINCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVSLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1SD family transposase"
     gene            complement(2239..3372)
                     /locus_tag="SSON53G_RS27665"
     CDS             complement(2239..3372)
                     /locus_tag="SSON53G_RS27665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="WP_000703236.1"
                     /translation="MKISIIGNTANAMILFRLDLIKTLTKKGISVYAFATDYNDSSKEI
                     IKKAGAIPVDYNLSRSGINLAGDLWNTYLLSKKLKKIKPDAILSFFSKPSIFGSLAGIF
                     SGVKNNNAMLEGLGFLFTEQPHGTPLKTKLLKNIQVLLYKIIFPHINSLILLNKDDYHD
                     LIDKYKIKLKSCHILGGIGLDMNNYCKSTPPTNEISFIFIARLLAEKGVNEFVAAAKKI
                     KKTHPNVEFIILGAIDKENPGGLSESDVDTLIKSGVISYPGFVSNVADWIEKSSVFVLP
                     SYYREGVPRSTQEAMAMGRPILTTNLPGCKETIIDGVNGYVVKKWSHEDLAEKMLKLIN
                     NPEKIISMGEESYKLARERFDANVNNVKLLKILGIPD"
                     /name="glycosyltransferase family 4 protein"
     gene            complement(3372..3575)
                     /locus_tag="SSON53G_RS34850"
     CDS             complement(3372..3575)
                     /locus_tag="SSON53G_RS34850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000231475.1"
                     /translation="MVFLDKFNFGVSGLSGNLWLMEKWELKNIFNFLLKGKIMAVPAIL
                     FSLIKYERRCALTKKNKGKGNK"
                     /name="hypothetical protein"
     gene            complement(3590..4180)
                     /locus_tag="SSON53G_RS27670"
     CDS             complement(3590..4180)
                     /locus_tag="SSON53G_RS27670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_011898198.1"
                     /translation="MKNHKVSIIIPCFNNGKTIERCVLSALKQTHRNIEIICINDGPSD
                     NSWNILKKLSSLYGNVFAFNNEDNSGPSFSRIKGVSLSTGHFLSFLDADDYWHPKKLEL
                     QLSFINDENLDFLGSTCSIGEKNNQEINQGIKKEHLKLKIISFNMMLFKNYFQTPAVIM
                     KRDIFIPFNENQRFSEDYMSWLVIVYNKKTNVD"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(4340..5065)
                     /locus_tag="SSON53G_RS27675"
     CDS             complement(4340..5065)
                     /locus_tag="SSON53G_RS27675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_241557509.1"
                     /translation="MWFIFFFKRKPIHLSYLIALGLLFVSSFLLIKLFQLGFGDWGRLT
                     VPVFYDNSWSYFPLGYESSSDPNVLAFMLCIGSIYMFFTLPNKYTINIIFILSMMCSFL
                     TLSRSSFLSFFISFLIVSVYSFFVLKKTQLRLLALIMISLAMSFVVMKNIPAVVTENNG
                     ASFVTEPNITERIVSKASDSDRIARFKNAIVEISNLKTMFIGNGIGYSTLTQDPHNFYL
                     STIIDSSILILAIVMMVLY"
                     /name="hypothetical protein"
     gene            5982..6167
                     /locus_tag="SSON53G_RS34855"
     CDS             5982..6167
                     /locus_tag="SSON53G_RS34855"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_243461349.1"
                     /translation="MVGALNTLISTAVVFYLSGVGAINIKRALNEIKDGFSVFLYKSSQ
                     GIMITMSSSLLVFLGG"
                     /name="hypothetical protein"
     gene            6343..>6408
                     /locus_tag="SSON53G_RS33775"
                     /pseudo=""
     CDS             6343..>6408
                     /locus_tag="SSON53G_RS33775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            6485..6757
                     /locus_tag="SSON53G_RS27685"
     CDS             6485..6757
                     /locus_tag="SSON53G_RS27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000019161.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSCT
                     VCFWETFHKMSPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
                     /name="hypothetical protein"
     gene            6738..7946
                     /locus_tag="SSON53G_RS27690"
     CDS             6738..7946
                     /locus_tag="SSON53G_RS27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_000198563.1"
                     /translation="MTRSGGDFQPRPLKRLFTTNQCWTSFLDASGLRDIEVEAVTKMLA
                     CGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVH
                     LVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNCHP
                     HVHVSVTCGCLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSHITTE
                     SQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFR
                     YLDHKTGETAMETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGM
                     DKPAPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNARDISLLRY
                     MPA"
                     /name="IS91 family transposase"
     gene            8016..8360
                     /locus_tag="SSON53G_RS27700"
     CDS             8016..8360
                     /locus_tag="SSON53G_RS27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020560602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_001188542.1"
                     /translation="MQREKTPEWREKQKISRGIRRGQGYRLVFQFPIRQAEKRAEAKAA
                     RKARDHALYQSAGLLIMAGLVDSQTGKPVDDPAKLLGALVSLHELSRDNPKWEEWKLKG
                     QVLLNQKKED"
                     /name="conjugal transfer protein TraD"
     Typing          2..957
                     /name="rep_cluster_1155"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtgtt taaagcaaat gaattagcag taagccgtta tgatctaact
       61 gaacatgaaa ccaagctaat tctgttttgc gttgcaaagt tgaaccctac aattgaaaac
      121 ccaacaaggg atgaattaac agttaaattc tcatgttctg aatatgcacg cactatgggc
      181 ttaagttatg aaaatgcttg gggaagattg aacagtgcaa ctagtgattt atttaaacgc
      241 tctgttgaat taatttatcc tacaggagca gtttctaaac gaattttcaa ttggacggaa
      301 tacgctgagt tcaatagaga agaacaaact gtaacattag tatttagctc atatattcaa
      361 ccacttttat ttcatttaaa aaaattcatt aaatacaacc ttgagcatgt taaagccttt
      421 gaaaataaat actcaatgcg aatatatgaa tggttactaa aggagctttc acaaagaaaa
      481 acgcataggg gaaacattga gataagtatc aaggaattta agtttatgct catgcttgaa
      541 aaaaactacc ctttatatgc agaattgaat cgctggatcc taaaaccagt tacaaatgac
      601 ttaaacactt acagcaatat gaagttgact attgaaaaac gcggtcgtcc tgctgacaca
      661 ctgatctttc aagttgaact ggataaacaa attgaccttg tgactgaact agcaaaagat
      721 cccgcatcaa aaaaagaaga taagacaatc cgtttaacgc ctgaaaatcg tcttcatgag
      781 gggctaaaaa caacattgca tgatgcttta actgcaaaaa ttcaactgac tagttttgaa
      841 gcaaaattcc tgagcgatat gcaaagcaag tacgatctta acggctcatt tacatggctg
      901 actcaaaagc aacgaactac gctagagaaa attctggcta aatacgggcg gatatgaggt
      961 atctatggca ctggtatcag ttacagaggc agccagacta actggaaaaa acagagcaat
     1021 aatacatcgc tatattaagc aaggaaaact gtcgcaaatt tctgatgcaa caaatacaaa
     1081 aaaagtagat acatcagagc ttattcgtgt atttggttca ctaactgcga cacatgcaac
     1141 actgtcgcaa agcgacgcaa tgcagcacga tgcaacacct aaaacagatc aatccgttgc
     1201 actgttacaa caaaaaattg cacttttaga acaactactt gaaagcaaaa ataacgaatt
     1261 gcaacgtcag gacgagcggt gatgctgcca acttactgat ttagtgtatg atggtgtttt
     1321 tgaggtgctc cagtggcttc tgtttctatc aactgtccct cctgttcagc tactgacggg
     1381 gtggtgcgta acggcaaaag cactgccgga catcagcgct atctctgctc tcactgccgt
     1441 aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc
     1501 attgatatgg ccatgaatgg cgttggatgc cgggcaactg cacgcattat gggcgttagc
     1561 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
     1621 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtgggga tacgtcgggg
     1681 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag gctccggaag acggttgttg
     1741 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtccc
     1801 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
     1861 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
     1921 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
     1981 tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa gttagagtca
     2041 ttaccaacac tgatataatg gcggtttaga acctatctca atagggatta attaccgcat
     2101 aaaaaatata ttatcactgt aaccaaaacc ttatacagcc tgacttttaa acatcgatat
     2161 ggctgggtaa ggtcatgaat ttagctccta ttgagatagg ttcttagttc cgaatgaatc
     2221 agagccgctt ttcgtttatt aatcaggaat ccctagtatt tttaataact ttacattatt
     2281 tacatttgca tcgaatcttt ctcttgctaa cttataactt tcttctccca tactgattat
     2341 tttttcagga ttattaatta acttcagcat tttttctgca agatcttcat gtgaccattt
     2401 ttttacaaca tatccattca caccatcaat aattgtttct ttgcagcctg gtaaattagt
     2461 agttaaaatc ggcctcccca tagccatcgc ttcttgtgta ctacgaggaa ctccctctcg
     2521 ataataggaa ggaagaacaa atacgcttga tttttcaatc caatcagcca cattagaaac
     2581 aaatccggga taagaaataa ctcctgattt aattaaagta tctacgtcag attcagataa
     2641 ccctccgggg ttttccttat ctatagcgcc aagtataata aattcaacat tgggatgtgt
     2701 tttttttatt ttttttgcgg cagcaacaaa ctcattgact cctttttctg ctagcaaacg
     2761 agctataaaa atgaatgata tttcatttgt tggtggcgtg cttttacagt aattattcat
     2821 atctaaacca atgccaccaa gaatatggca agattttaat tttattttgt atttatctat
     2881 caaatcatga taatcatcct tgttaaggag tattaatgag ttgatatgtg gaaatattat
     2941 tttatacagg agaacctgga tatttttaag taactttgtt tttaacggag ttccatgtgg
     3001 ctgctctgta aataaaaaac ctaacccctc aagcatagcg ttattatttt taacgcctga
     3061 aaaaatacca gccaacgatc caaagataga gggctttgaa aaaaaagata aaatagcatc
     3121 tggttttatc ttctttagtt ttttacttaa taagtaagta ttccataaat caccagcaag
     3181 gttaatacca ctgcgactta aattataatc aacaggaatg gcgcctgctt tttttattat
     3241 ttccttggat gaatcattat agtcagtagc aaaagcatag actgaaatcc ctttcttggt
     3301 tagtgttttt attaaatcca atctaaacaa aatcatagca tttgctgtgt tccctattat
     3361 tgagatcttc attatttatt acccttacct ttatttttct ttgttaaagc gcatcttctt
     3421 tcatatttta tcaaagaaaa caagatcgca ggcactgcca ttattttacc tttcaacaag
     3481 aagttaaata tattttttaa ctcccacttc tccatcaacc ataaattacc actcaaccct
     3541 gacactccaa agttaaattt atcgagaaaa accaaatccc ttccatatat taatccacat
     3601 ttgttttttt attataaacg ataacaagcc atgacatgta gtcctctgaa aaacgctgat
     3661 tctcattaaa tggaataaaa atatctcttt tcataatgac agctggagtc tggaaataat
     3721 tcttaaacaa catcatgtta aatgaaatta tttttaattt taaatgttct tttttaattc
     3781 cttggttaat ttcttggtta tttttctcac caatggaaca cgttgaacct aaaaaatcca
     3841 agttttcatc attaataaat gatagttgta attctagttt ttttggatgc cagtaatcat
     3901 ctgcatccaa aaatgacaaa aaatgccccg tagatagtga aaccccttta atcctggaaa
     3961 atgacggccc tgaattgtcc tcattattaa aagcaaaaac atttccatac aatgagctta
     4021 atttctttaa aatattccag ctattatctg acggtccatc atttatacaa ataatctcta
     4081 tatttctatg ggtttgtttt aatgcggaaa gaacgcatct ttctatagtt tttccgttat
     4141 taaaacaggg gatgataata ctaaccttat ggtttttcat tgctcactct tcttacttat
     4201 atttacaaag gtaaataata atataataac aaagtatagg actctcactt gccagtagaa
     4261 cagagaaatg aacatatagt atatagtcaa gaacataccc ccaaaataac atttgatttt
     4321 gcgggggttc ttgaaaccat taatatagca ccatcattac aattgctaat atcaaaatac
     4381 tagaatcaat aatagttgat aagtaaaaat tatgtggatc ctgtgttaat gtactatagc
     4441 caataccatt accaataaac attgtcttta gattacttat ttcaacaata gcatttttaa
     4501 atctggctat tctatcacta tcacttgctt tactaactat tcgttctgtt atatttggtt
     4561 ctgtgacaaa tgaagctcca ttattctcag tcactacagc gggaatgttt ttcatcacta
     4621 caaaagacat tgcaagtgaa atcataatta gtgccaacaa ccttagctgt gttttcttta
     4681 gcacaaagaa tgaataaaca cttacaataa gaaaggaaat aaaaaaagat aaaaaagaac
     4741 tccgtgataa ggtcaaaaat gaacacatca ttgacaatat gaatataata ttaattgtgt
     4801 atttatttgg taatgtgaaa aacatataga ttgagccaat gcataacata aaagctaaca
     4861 catttgggtc acttgaactt tcataaccca aaggaaaata agaccatgag ttatcataaa
     4921 aaacaggaac cgttaaacgc ccccagtcac caaagccaag ttgaaataat ttaatcaaca
     4981 aaaaagagct aacaaaaagc aggcctaaag caattaaata tgataaatgt attggttttc
     5041 ttttaaagaa gaaaataaac cacatagata ttactatata tacagcatga ctagtaatta
     5101 gagtgataga tttatgatta tatacaaaaa tgttaatgac aaggtaagcg actactaagc
     5161 acaggagaaa aatatgtgat ctttttataa aagactttct ttttataagc caatagcaac
     5221 ctaatagtgt acaggaaaag agagcgtata ctgtaaccac atgatcacta ttgactccca
     5281 tgaccacctg tttattccct cccataactg accacaatat aaaaggaaaa gtgcaaatgt
     5341 atagataaaa acacatagtg attcaactgg ttatttttat tgtagataag attcttctat
     5401 aatatcataa acatgatacc gcactcaaca tttttaacag atgatattgt ttatgaacag
     5461 acttttttct aatgctttct ggttaatggc atctcagatt ggcggccttc gaatctcctt
     5521 gatcgaactg ccattgcttg ccagaagact gggggctgat gaatatggaa aagttgtata
     5581 tgttttagct atttccataa tggcttctgt tttcatttag tatggattca atttcagtgc
     5641 tgtgaaaaaa aatcgtagga aggaaatatg gtaaatatat ttcacaaatt tcaacagatg
     5701 tttttcttgc taagataact cttagtatta tacctattat tttagctctc cccctaatat
     5761 acacaacatc tagcggaata tctataccta ataattggtt tgtatggatt attttattta
     5821 tcttatcttt tggattcact ccgttctggt attacatatc aatagaaaat ataattcccc
     5881 ccgcaatact cgatctttta ctaagagtga caggtttaat tctcattgtt atttttgtta
     5941 actccccgag tgacgcaaaa accacaatag ccattcaatc aatggtagga gcgttaaata
     6001 cattaatatc aactgctgtt gttttttatt tgtcgggagt aggagcgatt aatattaaaa
     6061 gagcgcttaa tgaaattaaa gatgggttta gtgtattttt gtataaaagt tcacaaggaa
     6121 taatgataac catgtcatca tcattattag tttttttggg gggctaaatc tgttgttttt
     6181 tttgttccat ctgaaaaagt aactaaggtt ataacaacat taatgataac aatgctaaac
     6241 gtagcatatc cgcgtttcgt taaaaatctt agtgatagca aaaacggtga tgcctctaat
     6301 tagttgaatc tgatgtataa tgcgggcttt tgaggttctt tcatggccag cgttaacatt
     6361 cattgtcctc gttgtcagtc tgcacaggtc taccgccatg gtcagaacca cggcagcctg
     6421 gcggctgcaa atcggggtgt ggcagaatat gagctttcag agtaaccatc cagaatgagt
     6481 tcccatgtca cacaacctgg agcaccagaa agtccacacc agaatggtga aagaggttct
     6541 gaaagccgtc gccagagcca ataatcaccc ttatcagtcc gtatttacag actttatcgc
     6601 cggtcatccc tcctgcacgg tctgtttctg ggaaaccttc cacaaaatgt ccccggattc
     6661 accgtacgaa tacgtcacgt tctgccatac ctgccgtcgt tttgatttat acgaaacaga
     6721 agcggagatg aaggcggatg acccgaagtg gtggtgattt tcagccccga ccgctgaaac
     6781 gcctgttcac aaccaaccag tgctggacat ccttcctgga tgcgagcggt ctgcgcgata
     6841 tcgaggttga ggctgtcaca aaaatgctgg cctgcggcac gcggatactg ggcgtgaagg
     6901 aatacaactg cgataaacct gagtgccccc acgtcagata cgtcactaac tcatgcggca
     6961 gccgtgcctg cccgtcctgc ggaaaaaagg ccacagacct gtggatagcg acacagctga
     7021 atcgtcttcc tgactgcgac tgggtacatc tggtcttcac cctgccggac acgctgtggc
     7081 cggtgttcga aagcaaccgc tggctgctga atgacgtgtg ccgtctggcg gtggagaatc
     7141 tgctgtatgc cgcccggaaa cgggggcagg aacccggtat cttctgcgcc atccacacgt
     7201 atggccgtcg tctcaactgt catccgcatg tacatgtgtc tgtaacctgt ggatgtctga
     7261 ataagcatgg tcagtggaaa aagctgagct tcctgaaaga cgcgatgcgt tcgcggtgga
     7321 tgtggaatat gcggcagctg cttctgaaag cgtggtcaga ggggctggcg atgccggagt
     7381 cgttgtcaca tatcacgacg gaatcacagt ggagaagcct ggtgctgaaa gccggcggaa
     7441 aatactggca tgtgtacatg tcgaagaaga cagccggcgg gaggaatacg gcgcgctacc
     7501 tgggtcgtta tctgaagaag ccgccgatag cggcctcccg gctggcacat tacaacggag
     7561 gggcgagcct gagcttccgt tacctggacc acaaaacggg agaaacggcg atggaaacgc
     7621 tgacacagcg tgagctggtc gcgaggctga aacagcacat cccggagaag ttttttaaga
     7681 tggtgaggta cttcgggttc cttgccaacc gtgtgtgtgg agagaagctg ccgcaggtgt
     7741 accgtgctct ggggatggat aaaccggcac cggtggcgaa ggtgtgctat gcacaaatgg
     7801 tgaagcagtt cctgagtcgt gacccgttcg aatgcgtgct gtgtggcggc cggatggtat
     7861 accgccgggc catcgcggga ctgaatgtgg aggggctgaa gaaaaacgcg cgggatatca
     7921 gtctgctgag gtatatgccg gcctgacaca ggctaagtgt gcccgggaat cggtgcaagt
     7981 ataaaaaata gcctgttact gcataaaaac tgaccatgca gagagagaaa acaccggagt
     8041 ggagggaaaa gcagaaaatc tcacgtggca taaggcgcgg acaggggtat cgcttagttt
     8101 ttcaatttcc tatacgtcaa gcagagaagc gtgcagaagc caaagctgcc cgtaaagcac
     8161 gtgaccatgc cctttatcaa tcagcggggt tactcatcat ggcggggtta gtggacagtc
     8221 aaacgggtaa accggtagat gaccccgcaa aactactggg tgcattagtc agcttgcatg
     8281 aattatcccg tgataatccc aaatgggaag aatggaaatt gaaggggcag gtattactca
     8341 atcagaaaaa ggaagattag agcacagtgt taataccctc taaaacgcca cagaaagtcc
     8401 gtggcgaggt tttgttggtt cgataatgga atttatcacc taatactgaa aacatggcac
     8461 agaagagcaa acaaccacct tcctgattga gccgtgagcg aagcgaaccc aatgacacga
     8521 aaaaaacagc ggcgctgtgg ctggatggtc ggagacaaaa gaaaggggca gcgcgttgcc
     8581 cgagcaaagc gagggagcta ctgaggtgtt ttaggtttgt cttgaaaagg agcaaacgtt
     8641 gtttgcgaca tccttttaac tacggcaaat cgacggcaat tttctgattt atccacaggg
     8701 ctgggatcta ttcttttttt atctcttatt ctttctttat tctaatgatt ttaacttatt
     8761 gttttttaat aaaaaaaaaa acaataaatg taggaaaaat taggctagat gtaggaaaaa
     8821 ttaggctagc agtgggaaag atgtaggaaa aattaggtca ttcacaatca aacacaaaaa
     8881 atgtagttga tttaaatcgc tacaccatta tgattcatgt agcgatttaa attactacat
     8941 aatggtgatt agc
//
