LOCUS       NC_016009              19557 bp    DNA     circular CON 03-MAY-2024
DEFINITION  Enterococcus faecium plasmid pM7M2, complete sequence.
ACCESSION   NC_016009
VERSION     NC_016009.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14226061
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19557)
  AUTHORS   Li,X., Alvarez,V., Harper,W.J. and Wang,H.H.
  TITLE     Persistent, toxin-antitoxin system-independent, tetracycline
            resistance-encoding plasmid from a dairy Enterococcus faecium
            isolate
  JOURNAL   Appl Environ Microbiol 77 (20), 7096-7103 (2011)
   PUBMED   21784909
REFERENCE   2  (bases 1 to 19557)
  AUTHORS   Li,X. and Wang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2011) Food Science and Technology, The Ohio State
            University, 2015 Fyffe Rd, Columbus, OH 43210, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009957395.2-RS_2024_05_03
            Annotation Date                   :: 05/03/2024 03:56:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 20
            CDSs (total)                      :: 20
            Genes (coding)                    :: 18
            CDSs (with protein)               :: 18
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JF800907.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="M7M2"
                     /isolation_source="cheese"
                     /db_xref="taxon:1352"
                     /plasmid="pM7M2"
                     /identified_by="Xinhui Li"
     gene            <1..708
                     /locus_tag="HS798_RS00005"
                     /old_locus_tag="PM7M2_p01"
                     /pseudo=""
     CDS             <1..708
                     /locus_tag="HS798_RS00005"
                     /old_locus_tag="PM7M2_p01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /name="conjugal transfer protein"
     gene            953..1069
                     /locus_tag="HS798_RS00010"
                     /old_locus_tag="PM7M2_p02"
     CDS             953..1069
                     /locus_tag="HS798_RS00010"
                     /old_locus_tag="PM7M2_p02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="WP_001791010.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
                     /name="tetracycline resistance determinant leader peptide"
     gene            1085..3004
                     /gene="tet(M)"
                     /locus_tag="HS798_RS00015"
                     /old_locus_tag="PM7M2_p03"
     ARG             1086..3001
                     /name="tet(M)"
                     /gene="tet(M)"
                     /gene_name="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3198..4574
                     /gene="tet(L)"
                     /locus_tag="HS798_RS00025"
                     /old_locus_tag="PM7M2_p04"
     ARG             3199..4571
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5138..6400
                     /gene="mobV"
                     /locus_tag="HS798_RS00030"
                     /old_locus_tag="PM7M2_p07"
     CDS             5138..6400
                     /gene="mobV"
                     /locus_tag="HS798_RS00030"
                     /old_locus_tag="PM7M2_p07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000119405.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="mobV"
     gene            6645..>7097
                     /locus_tag="HS798_RS00035"
                     /old_locus_tag="PM7M2_p09"
                     /pseudo=""
     CDS             6645..>7097
                     /locus_tag="HS798_RS00035"
                     /old_locus_tag="PM7M2_p09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(7152..7832)
                     /locus_tag="HS798_RS00040"
                     /old_locus_tag="PM7M2_p10"
     CDS             complement(7152..7832)
                     /locus_tag="HS798_RS00040"
                     /old_locus_tag="PM7M2_p10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(8265..8477)
                     /locus_tag="HS798_RS00045"
                     /old_locus_tag="PM7M2_p11"
     CDS             complement(8265..8477)
                     /locus_tag="HS798_RS00045"
                     /old_locus_tag="PM7M2_p11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002324171.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            complement(8498..9349)
                     /locus_tag="HS798_RS00050"
                     /old_locus_tag="PM7M2_p12"
     CDS             complement(8498..9349)
                     /locus_tag="HS798_RS00050"
                     /old_locus_tag="PM7M2_p12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002287227.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            9532..10491
                     /locus_tag="HS798_RS00055"
                     /old_locus_tag="PM7M2_p14"
     CDS             9532..10491
                     /locus_tag="HS798_RS00055"
                     /old_locus_tag="PM7M2_p14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="WP_000222573.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHCLNRSRQTIHKVYLFF
                     KQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIRCS
                     ARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSITF
                     DCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDESF
                     IQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
                     /name="IS30 family transposase"
     gene            complement(10515..10811)
                     /locus_tag="HS798_RS00060"
                     /old_locus_tag="PM7M2_p15"
     CDS             complement(10515..10811)
                     /locus_tag="HS798_RS00060"
                     /old_locus_tag="PM7M2_p15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            complement(10946..12439)
                     /locus_tag="HS798_RS00065"
                     /old_locus_tag="PM7M2_p16"
     CDS             complement(10946..12439)
                     /locus_tag="HS798_RS00065"
                     /old_locus_tag="PM7M2_p16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            13051..14004
                     /locus_tag="HS798_RS00070"
                     /old_locus_tag="PM7M2_p18"
     CDS             13051..14004
                     /locus_tag="HS798_RS00070"
                     /old_locus_tag="PM7M2_p18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            13976..14251
                     /gene="prgO"
                     /locus_tag="HS798_RS00075"
                     /old_locus_tag="PM7M2_p19"
     CDS             13976..14251
                     /gene="prgO"
                     /locus_tag="HS798_RS00075"
                     /old_locus_tag="PM7M2_p19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            14444..14698
                     /locus_tag="HS798_RS00080"
                     /old_locus_tag="PM7M2_p20"
     CDS             14444..14698
                     /locus_tag="HS798_RS00080"
                     /old_locus_tag="PM7M2_p20"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_014088863.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKQNARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            15104..16266
                     /locus_tag="HS798_RS00090"
                     /old_locus_tag="PM7M2_p22"
     CDS             join(15104..15380,15380..16266)
                     /locus_tag="HS798_RS00090"
                     /old_locus_tag="PM7M2_p22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_086953888.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANKHL
                     YPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSKRGI
                     QISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLT
                     YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFHTDRGK
                     EFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLEL
                     FDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
                     /name="IS3 family transposase"
     gene            16442..16939
                     /locus_tag="HS798_RS00095"
                     /old_locus_tag="PM7M2_p23"
     CDS             16442..16939
                     /locus_tag="HS798_RS00095"
                     /old_locus_tag="PM7M2_p23"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_228780308.1"
                     /translation="MCEKWAADITYIPTKKNGWCYLSSIMDLHTKRIISYTFSKRMTVD
                     CVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGI
                     ESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEK
                     MA"
                     /name="IS3 family transposase"
     gene            complement(17412..17984)
                     /locus_tag="HS798_RS00100"
                     /old_locus_tag="PM7M2_p26"
     CDS             complement(17412..17984)
                     /locus_tag="HS798_RS00100"
                     /old_locus_tag="PM7M2_p26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000170424.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(18000..18605)
                     /locus_tag="HS798_RS00105"
                     /old_locus_tag="PM7M2_p27"
     CDS             complement(18000..18605)
                     /locus_tag="HS798_RS00105"
                     /old_locus_tag="PM7M2_p27"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     gene            complement(18803..19483)
                     /locus_tag="HS798_RS00110"
                     /old_locus_tag="PM7M2_p28"
     CDS             complement(18803..19483)
                     /locus_tag="HS798_RS00110"
                     /old_locus_tag="PM7M2_p28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     ARG             3289..4574
                     /name="tet(45)"
                     /gene="tet(45)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="76.35"
                     /coverage="93.45"
     Typing          complement(10947..12439)
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5139..6397
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttgatactc atcatgtaga aaattttgta gagaactttg cgaaagtcta ctattcatgg
       61 gagcaatccg ataagtccat tgataatcga atggaaagtc taaaaggcta tctgacagat
      121 gaacttcaag ctctcaatgt tgatacagta cgcaaagata ttcctgtatc gtcttctgta
      181 agaggatttc agatatggac ggtagagcca actggcgaca atgagtttaa tgtaacctac
      241 agtgtagacc agctcattac agagggagaa aatacaaaga ccgtccactc tgcttatata
      301 gtgagtgtct atgtagatgg ttctggaaat atggtactgg ttaagaatcc gaccattacc
      361 aacataccta agaaatcaag ttataaacca aaagccattg aaagtgaggg gacggttgat
      421 tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct ctatcctaca
      481 gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc aatcggaaaa
      541 gagtacatct ttcaagaact ggtaaatcct attcacaatc gtaaggataa tcaagtcacg
      601 gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt atctcaattt
      661 gatttggtac ttgaaaagaa cgggagtaat tggaagattg taaaataaca aatattggta
      721 catgattaca gatactttgt aatcatgtac tctttttgat aaaaaattgg agattccttt
      781 acaaatatgc tcttacgtgc tattatttaa gtgactattt aaaaggagtt aataaatatg
      841 cggcaaggta ttcttaaata aactgtcaat ttgatagcag gaacaaataa ttagatgtcc
      901 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
      961 aaagtatcca gagaatatct gtatgctttg tatacctatg gttatgcata aaaatcccag
     1021 tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa
     1081 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt
     1141 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg
     1201 tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg
     1261 aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat
     1321 ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat
     1381 ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat
     1441 ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
     1501 ggtttatcag gatattaaag agaaactttc tatggaaatt ataatcaaac agaaagtaga
     1561 gctgcaccct aatatgtgtg tgatgagctg tacggaacct gagcaatggg atgtggtaat
     1621 agaaggaaat gatgaccttt tagagaaata tatgtccggt aaatcattag aagcattaga
     1681 actcgaacaa gaggaaatca gaagatttca gaattgctcc ttgtaccctg tttatcatgg
     1741 aagcgcaaaa agcaacatag ggattgagca gcttatagaa gtgataacga ataaatttta
     1801 ttcatcaaca tacagaaaga agtctgaact ttgcggaaat gtcttcaaaa ttgaatattc
     1861 ggaagaaaga caacgtcttg catatgtacg cctttatggc ggaatcctgc atttgcggga
     1921 ttcggttaga atatcggaaa aggaaaaaat aaaaattaca gaaatgtata cttcaataaa
     1981 tggtgaatta tgtaaaattg ataaggctta ttccggggaa attgttattt tgcaaaatga
     2041 gtttttgaag ctaaatagtg ttcttggaga tacaaagcta ttgccacaga gagagagaat
     2101 tgaaaatccg ctccctctgc tgcaaacaac tgttgaaccg agcaaacctc aacaaaggga
     2161 aatgttactt gatgcacttt tagaaatctc cgacagtgac ccgcttctac aatattatgt
     2221 ggattctacg acacatgaaa ttatactttc tttcttaggg aaagtacaaa tggaagtgat
     2281 tagtgcactg ttgcaagaaa agtatcatgt ggagatagaa ctaaaagagc ctacagtcat
     2341 ttatatggag agaccgttaa aaaatgcaga atataccatt cacatcgaag tgccgccaaa
     2401 tcctttctgg gcttccattg gtttatctgt atcaccgctt ccgttgggaa gtggaatgca
     2461 gtatgagagc tcggtttctc ttggatactt aaatcaatca tttcaaaatg cagttatgga
     2521 agggatacgc tatggttgcg aacaaggatt atatggttgg aatgtgacgg attgtaaaat
     2581 ctgttttaag tatggcttat actatagccc tgttagtacc ccagcagatt ttcggatgct
     2641 tgctcctatt gtattggaac aagtcttaaa aaaagctgga acagaattgt tagagccata
     2701 tcttagtttt aaaatttatg cgccacagga atatctttca cgagcataca acgatgctcc
     2761 taaatattgt gcgaacatcg tagacactca attgaaaaat aatgaggtca ttcttagtgg
     2821 agaaatccct gctcggtgta ttcaagaata tcgtagtgat ttaactttct ttacaaatgg
     2881 acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt actaccggtg aacctgtttg
     2941 ccagccccgt cgtccaaata gtcggataga taaagtacga tatatgttca ataaaataac
     3001 ttagtgtatt ttatgttgtt atataaatat ggtttcttgt taaataagat gaaatatttt
     3061 ttaataaaga tttgaattaa agtgtaaagg aggaattaat tatgaagtga aatgaatgta
     3121 acagggttca attaaaagag ggaagcgtat cattaaccct ataaactacg tctgccctca
     3181 ttattggagg gtgaaatgtg aatacatcct attcacaatc gaatttacga cacaaccaaa
     3241 ttttaatttg gctttgcatt ttatcttttt ttagcgtatt aaatgaaatg gttttgaacg
     3301 tctcattacc tgatattgca aatgatttta ataaaccacc tgcgagtaca aactgggtga
     3361 acacagcctt tatgttaacc ttttccattg gaacagctgt atatggaaag ctatctgatc
     3421 aattaggcat caaaaggtta ctcctatttg gaattataat aaattgtttc gggtcggtaa
     3481 ttgggtttgt tggccattct ttcttttcct tacttattat ggctcgtttt attcaagggg
     3541 ctggtgcagc tgcatttcca gcactcgtaa tggttgtagt tgcgcgctat attccaaagg
     3601 aaaatagggg taaagcattt ggtcttattg gatcgatagt agccatggga gaaggagtcg
     3661 gtccagcgat tggtggaatg atagcccatt atattcattg gtcctatctt ctactcattc
     3721 ctatgataac aattatcact gttccgtttc ttatgaaatt attaaagaaa gaagtaagga
     3781 taaaaggtca ttttgatatc aaaggaatta tactaatgtc tgtaggcatt gtatttttta
     3841 tgttgtttac aacatcatat agcatttctt ttcttatcgt tagcgtgctg tcattcctga
     3901 tatttgtaaa acatatcagg aaagtaacag atccttttgt tgatcccgga ttagggaaaa
     3961 atataccttt tatgattgga gttctttgtg ggggaattat atttggaaca gtagcagggt
     4021 ttgtctctat ggttccttat atgatgaaag atgttcacca gctaagtact gccgaaatcg
     4081 gaagtgtaat tattttccct ggaacaatga gtgtcattat tttcggctac attggtggga
     4141 tacttgttga tagaagaggt cctttatacg tgttaaacat cggagttaca tttctttctg
     4201 ttagcttttt aactgcttcc tttcttttag aaacaacatc atggttcatg acaattataa
     4261 tcgtatttgt tttaggtggg ctttcgttca ccaaaacagt tatatcaaca attgtttcaa
     4321 gtagcttgaa acagcaggaa gctggtgctg gaatgagttt gcttaacttt accagctttt
     4381 tatcagaggg aacaggtatt gcaattgtag gtggtttatt atccataccc ttacttgatc
     4441 aaaggttgtt acctatggaa gttgatcagt caacttatct gtatagtaat ttgttattac
     4501 ttttttcagg aatcattgtc attagttggc tggttacctt gaatgtatat aaacattctc
     4561 aaagggattt ctaaatcgtt aagggatcaa ctttgggaga gagttcaaaa ttgatccttt
     4621 ttttataaca ggaattcaaa tctttttgtt ccattaaagg gcgcgattgc tgaataaaag
     4681 atacgagaga cctctcttgt atctttttta ttttgagtgg ttttgtccgt tacactagaa
     4741 aaccgaaaga caataaaaat tttattcttg ctgagtctgg ctttcggtaa gctagacaaa
     4801 acggacaaaa taaaaattgg caagggttta aaggtggaga ttttttgagt gatcttctca
     4861 aaaaatacta cctgtccctt gctgattttt aaacgagcac gagagcaaaa cccccctttg
     4921 ctgaggtggc agagggcagg tttttttgtt tcttttttct cgtaaaaaaa agaaaggtct
     4981 taaaggtttt atggttttgg tcggcactgc cgacagcctc gcagagcaca cactttatga
     5041 atataaagta tagtgtgtta tactttactt ggaagtggtt gccggaaaga gcgaaaatgc
     5101 ctcacatttg tgccacctaa aaaggagcga tttacatatg agttatgcag tttgtagaat
     5161 gcaaaaagtg aaatcagctg gactaaaagg catgcaattt cataatcaaa gagagcgaaa
     5221 aagtagaacg aatgatgata ttgaccatga gcgaacacgt gaaaattatg atttgaaaaa
     5281 tgataaaaat attgattaca acgaacgtgt caaagaaatt attgaatcac aaaaaacagg
     5341 tacaagaaaa acgaggaaag atgctgttct tgtaaatgag ttgctagtaa catctgaccg
     5401 agattttttt gagcaactgg atccaggaga acaaaaacga ttttttgagg aaagttataa
     5461 attattttcc gaacgatatg gcaagcaaaa tattgcttat gcaacagttc ataatgatga
     5521 gcaaacccct cacatgcatt taggtgttgt gcctatgcgt gatggaaaac tgcaaggaaa
     5581 aaatgtgttt aatcgtcaag aactgttatg gctacaagat aaattccccg agcatatgaa
     5641 aaaacagggt tttgagttga agcgtggtga acgtggctct gaccgtaaac atattgagac
     5701 agctaaattt aaaaaacaaa ctttggaaaa agagattgat tttctagaaa aaaatttagc
     5761 agttaaaaaa gatgaatgga ctgcttatag cgataaagtt aaatcagatt tagaagtacc
     5821 agcgaaacga cacatgaaaa gtgttgaagt gccaacgggt gaaaagtcca tgtttggttt
     5881 gggaaaagaa ataatgaaaa cagaaaagaa accaaccaaa aatgttgtta tatcggagcg
     5941 tgattataaa aacttagtga ctgctgcgag agataacgat aggttaaaac agcatgttag
     6001 aaatctcatg agtactgata tggcgagaga atataaaaaa ttaagtaaag aacatgggca
     6061 agttaaagaa aaatatagtg gtcttgtaga gcgatttaat gaaaatgtaa atgattataa
     6121 tgagttgctt gaagaaaaca agtctttaaa gtctaaaata agcgatttaa agcgtgatgt
     6181 gagtttaatc tatgaaagca ctaaggaatt ccttaaggaa cgtacagacg gcttaaaagc
     6241 ctttaaaaac gtttttaagg ggtttgtaga caaggtaaag gataaaacag cacaattcca
     6301 agaaaaacac gatttagaac ctaaaaagaa cgaatttgaa ctaactcata accgagaggt
     6361 aaaaaaagaa cgaagtcgag atcagggaat gagtttataa aataaaaaaa gcacctgaaa
     6421 aggtgtcttt ttttgatggt tttgaacttg ttctttctta tcttgataca tatagaaata
     6481 acgtcatttt tattttagtt gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta
     6541 aagtattgaa aacccttaaa attggttgca cagaaaaacc ccatctgtta aagttataag
     6601 tgaccaaaca aataactaaa tagatggggg tttcttttaa tattatgtgt cctaatagta
     6661 gcatttattc agatgaaaaa tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga
     6721 gaccatggag agaaaagaaa atcgctaatg ttgattactt tgaacttctg catattcttg
     6781 aatttaaaaa ggctgaaaga gtaaaagatt gtgctgaaat attagagtat aaacaaaatc
     6841 gtgaaacagg cgaaagaaag ttgtatcgag tgtggttttg taaatccagg ctttgtccaa
     6901 tgtgcaactg gaggagagca atgaaacatg gcattcagtc acaaaaggtt gttgctgaag
     6961 ttattaaaca aaagccaaca gttcgttggt tgtttctcac attaacagtt aaaaatgttt
     7021 atgatggcga agaattaaat aagagtttgt cagatatggc tcaaggattt cgccgaatga
     7081 tgcaatataa aaaaattggt tctgttgcaa agttttaaat aaagaataaa atcccttacg
     7141 gtatctatga tttaagctgg gattcccaat aataccttga tttcagtaca gaccgaaaac
     7201 ccgaagagag tgccttcttt tcgggttttc ttatataatc ctcgaatggc ttccatgcct
     7261 ttaatcgtgg tagaggcagt gcgtaaactt cgatagaatt tattgcgtct ctttactgga
     7321 cgatggtctt gttcaatcaa attattcagg tatttaatgg tacgatgttc tgtcccttga
     7381 taaaagccgt attcttttag tttcttaaag gcacttgtaa tagagggggc tttatctgtg
     7441 actacaacct tcggttcatc aaactgcttc actaaccgct taagaaaagc ataggctgct
     7501 tgtgtgtccc gttttttacg taaccaaata tccaaggtta aaccatctgc atcgatggct
     7561 cgatacaaat aatgccattt tcctttaatt ttgatgtacg tttcatccat tttccatgaa
     7621 taaaaggatt ttttattttt ctttttccaa atttgataga gtagtttgcc atattcttgc
     7681 acccaacgat aaatcgtcgt atgagaaacg ttaatgccac gatcatataa gatttcttga
     7741 acttcacgat agctaaggtt ataacgaaga tagtagccca cggctacaat aatcacatcc
     7801 tgctgaaatt gctttccttt aaaatgattc atcgtcattc ctcctgccat ctttttctat
     7861 tattctacct tatttgatag tagatttaaa actttgcaac agaaccgttc gtcacatctt
     7921 cgatcaattt ctcttacgat tttctcatcc acatttctta ttcttacttc catattaatt
     7981 cctccaattt aaaaaggata gcgaaaagga gcctatcctt tttgctatcc tactaccctc
     8041 cttttactat ccttcactat cctttcacta tcctattttt aaatattttg ttcccacatt
     8101 tatttttgag aaacgtaata ggcacagcct attggaacac tttttttaaa aagtgttccc
     8161 acgtttcctt atgctcttta caaataaagg cttttagaga cgttctggaa ttttagattc
     8221 cttgaattgt tcccattcat caatcaattg gctataagta gccattaatt tttttcttgc
     8281 tcttgttgct ttcgaatcaa caaaaaatca gtttgattcg ttaattctaa atattctgtt
     8341 cggtattcaa tgttcatcgt acccgtttta ctacatagta actgattatg acgttctttc
     8401 actttgaata actgctcctt ctttaaggtt tttaactcca tgccaactat aagcacaatc
     8461 ctgtatagag gaatcatttt caaacgattc tccataatta tttatctttc attggaagtt
     8521 ctaaatcact actgttccaa aaaatgtatg gtttgttttg acgaatatta cttccatcta
     8581 accacaattt tccgatgcca tatcgtaggc ttgcttcgac ttcataaaaa ttgtagatct
     8641 cttttcttcg atctattgct ttcatttcct ggccattttt taaaatagat ctgatgccct
     8701 taacagtaga ttcatggata ctcgtgtgaa cgatatccgt attgatcaat tcgtcatatc
     8761 ttccaaaatc aacattgtac tcataaccaa gaagtgtcgg aatcaggtca atttcaattt
     8821 ctttttcaat tgaacgttta ataagtttcg tggatacata cccgacaaaa aagagaataa
     8881 tacccacttc aaacaaatac atgtgtccat ttacaaagtg aataaacgtc aataaaaaat
     8941 ccttcactcc ttttgttgtt cttccagatt gttcaatttg ttttattaaa gtcgctagaa
     9001 ttgcatttac aacaattaaa tcgaataggc ttttgaaaag ttttcctgct gttaattttt
     9061 gcctggtaaa ttgcatgact aattgagtaa aaatagacaa tagcatgaca aaaatcaacg
     9121 tcattagcac tgtaaaaatg tctgatgctg caatcctatt gggatcattt atcaagtaat
     9181 aagccgttcc aaatactcca atcgagtaaa cggttaaatc aagaataagt cgtgccccgt
     9241 aactacaaag ttttcctaat ggccataatt gcaataccca aaatttcttt cgtttgctat
     9301 ttcttttcag aggaagccag gtgtgttcaa tccagtcgtc cgatttcatt tcgatttctt
     9361 ccctaaattc tttcctttaa cgttttcctt tgctacttta gcagcgatag gttgctcaat
     9421 gaaccaattt tttaagtacg ccgattgtaa aattaagcta gacaactaaa aaagtcattt
     9481 gtgctacact caaaatagtt ccgtccaaag aattataagg agtgagtaca aatgacctat
     9541 acccatctta caacggatga acttgtaatg atagagtctt atttcaaaat aaatcaatct
     9601 gttgcgaaaa ctgcgcattg cttgaatcgt tcaagacaaa cgatccataa agtatacctg
     9661 ttcttcaagc aaggaaaatc agccttagag tactatcaac agtataagaa aaacaaatca
     9721 aactgtggta gacgcccgct tgttttacct gaggaacaat cagaatatat tcaaagaaag
     9781 gttgttcaag gatggacacc cgatgtgatt gttggtcgtg cagcgtttcc tattcgttgt
     9841 tctgctcgta ccatttatcg tatgttcaaa aaggggctat ttaatccttc tgacttaccg
     9901 atgaaaggca agcgtaaacc aaatggacat caagaaagac gtggaaaaca agctttccgt
     9961 cgctctattc atgaacgtga aaaagattat agccaattct caaatgagtt tggtcacctt
    10021 gaaggtgaca ctatcgtagg tttgaaacat aaaagtgctg taattacctt agttgagcga
    10081 ttatcaaaag ttatcatcac attgaaaccg tgtggtagac aagcgattga tattgaaaac
    10141 aaattaaatc attggtttga atctgtaccg aaaaatctat tcaaatccat cacttttgat
    10201 tgtggaaagg aattttcaaa ttggaaacag atcagtaatg ccaatgatat tgccatttat
    10261 ttcgctgatc caggaacgcc gtctcaaaga ggcctaaacg agaattctaa cggattgtta
    10321 cgtagagatg gtttattgaa atctatggat ttcaattcag tagatgaatc ttttattcaa
    10381 tctgtcgcat cgaaacgaaa taatattcct agaaaatcac tgaattatcg aacacctttg
    10441 gaagtatttt tgagttacgt aagtattgat gatttgtcta acttaatttg acaattaaga
    10501 gtaaataaaa actactactg gatatacttc ttcctttttc ttaactcgtt agctatttta
    10561 tactcaatat actcctgctc taacacacac aaacaatcat atacctgatc catagtctcc
    10621 ccagaagcta aggacaaccc atgaagaaac tcaatcggca aagaagcaac tgttttttcc
    10681 ctggtaaccc aagaagacaa agtcccctgc ttaaaaccat aaagctcaca gaacacttcc
    10741 acagataatc ccaaacgacc aatgataaac acattaacag gatgtgaata gacataattt
    10801 tttaacgaca tacaacttcc tccatgatac ataagactgc atacgcagtt ataagtatac
    10861 cacctacacg tagctaggca aagggagcaa cacctacaga agtatcatag atatattgta
    10921 taaggcaatt tgcacctttt atttttcaac ctgtatctac ttcaaaaaga ttcacttctt
    10981 taagagggtt caagggggtt gataattgtc taaatgtctc ttctactaag ctctcgttga
    11041 gtccaaatac ctctgtgatc cttgttgaat aatctagtct cttttcttta cttagttgaa
    11101 taaccctcgc gaacaacgtt ttgactgaag caatgatcat tccgccattt cttcctcgtg
    11161 ttaccttgta gaagacttta ttctcctctc gtaactcatt taagatctta tcaagtgttc
    11221 gcttgggcat cgttagttgt gaagccaatt ctttttttga caggttcaaa taaggctcac
    11281 tagcaagact tttttcgctt agataagcca atagatcttc tttccattca tttttatgac
    11341 tatattttct ttgatctcgt ggctttttaa atttccacca gccacgtcgt tgaatgaaca
    11401 gatcattatt ttttaaggtt ggtgaaaccc attgctggca caattcaata atgaattctc
    11461 tgcttgctgc ttgataatta ccagaataag cactacgtac aatctttact aattctgctt
    11521 cagatagcgg ttcctgtaac cgttcgttga atgtgaacat attgtattcg caagtatcca
    11581 atgcataacc tgaagagtaa taagctaaag aaagcgtgaa aatcacatta tttcgaccaa
    11641 gacgtccttc tccgccgacg atgtttgctt tatgaagtaa catatcgaac cacggttcat
    11701 ctacttgacg aatttctttt ttctcaggaa agacaattag ttttcgttct cgatcttctt
    11761 ttgttttttg atctgattca agcttcatcg accattgaat ccagtcagca aaagaatact
    11821 gatagttcat ttctttaaaa acaatattat tttcttgtgg gaatcgagca attccgaagt
    11881 ggttacatcc tagatccact ccggacaatt tttcagccaa ctgtagacga attgtttttg
    11941 aaattttttt tgctacatcg atcaccttga aattgctttt ctttgtgaca tagcctggtg
    12001 tctttagtac aaaataaact tgataaccat gatctgattc taaaattagc gttggcatgt
    12061 agcccacttg atctaaacag gctaaaataa tttcgccttg atggttctct tttgaatcga
    12121 tatcaactac aaatgtgttg atttgactta agtttttctc agaatgacct ttaaccactg
    12181 ttcgataatc atccgcataa gtaccaaagc gataggtatt aggtgtccaa tgcgttagtt
    12241 tcttttcgtt ctctaaaata gcttcttccg aagtaatgac cataccacgc ccaacattca
    12301 ttaattgctt gctacggtac gcaaagatag caccactcct actggaatca atatgacctg
    12361 caggtttcat ggtgctgttt cgatatttgt atgtacgtaa gccgttttta agaatagctg
    12421 aataaatttc gctcaacatc gattgtaacg tcctttctca aacaaaaaga gaacacagtt
    12481 tagctttaat ctcagccata gtatgtgttc tctcttcagt gtttaagaat atttaattta
    12541 agtacactaa ataaacgttc ttctgttgag ttgctcgcct ttttttggta gaataaaggc
    12601 aatcggaaaa ggtatccgat caacttttaa tataagtagt tggtagctac ttacatattc
    12661 ttgagacgag cgtgaacaca tgtcctaacc atgtgattca gaaataattg attctgctaa
    12721 aatcaattat ttccgtctct tttttttatg taatttttct gtttaaagag taccactgct
    12781 tttgctactt tgtaaatagt gaattatctg tggtttctat agaattaagt ttacctcttt
    12841 tcactctaaa ttgtcaacgc aaaacgcaca aatatatttc gacattttga atatatctga
    12901 tatactgaat atagcaaata tatttagtat atccactata ttccttataa agtctgaatt
    12961 cttatcaatt tggagtatat tagatatatc gaatatatta agtatatgaa gtatactaaa
    13021 tatatttagt ataggaaatt ggagggaact atggaaaact tacggactgc atttaaaaca
    13081 tcagataaac taggaaaagt tgtaatgctt acaggtagaa aaggcggcat tggtaagact
    13141 acagacaatg atttattagc gattgtttct agtcaacttt ttgaaaaaga cgtgttgcta
    13201 attgattatg accaacaaag aaatactact tccaacattg gatcaacgta tcaaattact
    13261 agctttgatc gatccatgtc tgcagcaatc aaaaaagggg actgggtttc aggtattact
    13321 caagtaagtc cacatttata tattatggct ggctctcctg gatctgaaga acttaatgaa
    13381 tacttgtccg aaaaatatcc tgatcgtcgt aagagatcat tggccttcat taaaccttta
    13441 gaagaattga gaaaaaattt cgattatatc tttatcgact gcccaccatc aacagataat
    13501 gtggttcgtg cattcttaac tgctgcagat tatattattg caatgcaaga attaaaacgg
    13561 tacgcaatgg aaggaacaga agattttatt aataaagttt tagttccgat tgtcacgaat
    13621 tttgaagagt ctcatcttca aatcattgga attttgcctg ttcttttctc agtgcgacgt
    13681 tcctctcaac acgcaaacta tcaaaaaaca attgataagt atggtgaatc aaacattttt
    13741 aaaagcattg ttaaaggctc ggatcggtta gaaatgtatg gtgagaatgg tatccaatta
    13801 aatgattatg tagatcgaag atggtgggct atttttgcag acatctttac agaattagaa
    13861 gaaagaattg aatactatga acaacatggt gatattgaag gatttgatta tacacctaaa
    13921 tatgctgact caatggccaa tcatatccta cctcttggaa aggagattaa actaaatggc
    13981 attattacca acgaacaaag ataagaagaa aattggtgct gaagatattt caattaaaga
    14041 tgatattaat gtcgagttag gggaacgaaa gtacactgcg aaagaaagaa aaccagttca
    14101 agtagatcct cccgtactaa aaaccattcg ggatcttagt tatgcaaaag atattcctat
    14161 gtatgaaata gtcaaacttg caatggacgc ctatgttgat actttaaacg acgaggaacg
    14221 gattatatat aatagacgat caaaagatta aaatatagta agtatactag atatagtaaa
    14281 tatatttagt atacttacta tatttttagc tttattaatc ctaatccttc aaaatatgtg
    14341 tattgtttgg gggtatacaa catttggtgt tagtatcaaa tcttagacag cttttcgtag
    14401 agacaagaat aaattaaact tatctacgag aggatgaatt tttatgaccc gatatgaaga
    14461 aaattttaaa caaatgattg ttgaactgaa tcaaactgga cgttctgttc gagggttagc
    14521 gaaagaatat ggcttatctg aagcaacgat ttacaaatgg aagaatttat atttacctga
    14581 tcagtccaca ggactgactg gaaaagaagt agctgaactg agaaaacaaa atgctcgttt
    14641 aaatgaggaa cttgaaatct taaaaaaagc cgcagccata ttctctcgga aaacctaaat
    14701 tcgcttgttc aatttattga aaaatggtgt aaggattaca ctgtttcttt gttgtgtcgg
    14761 ttattagaaa tccctagaag tgtctattat ttttataaga ataaaccgtt gacagctaca
    14821 gaaatcagaa ataataagtt gaaaaagaag atttcaacaa ttttttttac gaataaacaa
    14881 cgctatggtg ccacaaagat ccatcaagtt ttattaaaag aagggatttc agtatctctt
    14941 aaacatgtcc aaaagctaat gaaacaatta aatttaaggt cgattgtagt taaaaaatat
    15001 agacctcaaa ggtctaataa accgatcatg gataggctac aattaactag acagaaaaat
    15061 taaggtgtgt agactagaag aaaacatacc aggaggaatt tttatgtcta agagaacacg
    15121 aagaactttt tcacaagaat tcaagcaaca aatcgtcaat ctttacttag ctggaaagcc
    15181 acgtgtagaa atcattcgag aatatgaact aacggcttca gcatttgaca aatgggtaaa
    15241 gcaatttaaa acgagtggtt cattcaaaga aaaagataat cttacgcctg aacaaaaaga
    15301 attgttagaa ctacgtaaaa gaaaccagca attagaaatg gaaaatgata ttttaaagca
    15361 agcagcgctg atattcggac gaagagacaa gtaatcgatg cgaataagca tctttaccct
    15421 atatcagcga tgtgcagaat attaggtcta tcacgtcagt cctattatta tcaatcaaaa
    15481 ccaaagaaag acgaatcaga acttgaagaa gtagtcgctg aagaatttat ccgcagccga
    15541 aaggcctacg gctcaagaaa aataaaaaaa gccttatcaa aacgaggcat tcagatcagc
    15601 cgacgaaaaa ttagtagaat catgaaaaat agaggattaa aatcgagcta tactgttgct
    15661 tattttaaag tacatcattc tacttgcaat gaagccaaaa cgacaaacgt attgaatcgt
    15721 aaattcttaa gagacaaccc attagaagcg atcgtaacag acttgactta tgtacgagtc
    15781 gggaaaaaat ggaattatgt ctgtttcatt ttggatctgt tcaatcgaga aattctcggc
    15841 tattcttgtg gagaacataa agatgccgtt ctagtaaaaa aagcatttag ccgtatcaaa
    15901 caacctctga cagaggttga gatttttcat actgatcgtg gaaaagagtt tgataaccaa
    15961 gctattgatg aattattaac aacttttgac atcaatcgat cattgagtca taaaggctgt
    16021 ccttttgata atgccgtagc tgaatcaact tataagtcgt tgaaagtaga atttgtctat
    16081 caatacacat ttgaaacctt acaacaattg gatttggagt tatttgacta tgtcaattgg
    16141 tggaaccacc ttcggttgca cggtacactt ggctacgaga caccggttgg ttaccgtaac
    16201 cagagattgg cgcagcgaat ccttgataat gagctcggat gtgctaacgc tagcgaggca
    16261 gtctaacttt aactgttagc tcctgccgaa gatcgtcaca tccgaggagg ctcattgtca
    16321 aggacaatcg gaatagcata cggaaagaag ttctgcacct tataaaattt gtcaaaaaaa
    16381 ctgttgccat tccatcattt caaaagagaa tctcttaaat caggactttt ctactgaaac
    16441 gatctgtgag aaatgggcag ctgacattac gtacattcct actaagaaaa atggttggtg
    16501 ttacttatct tcaatcatgg atttacacac gaaaagaatt attagttata cattttcaaa
    16561 acgaatgact gtggattgtg tcattcaaac attgaataaa gcaaaaatac attatcacat
    16621 tccagaagga atgattctac acactgactt gggcagccaa tacacagcaa gagaagtgga
    16681 acaatggctt aaaaccaaca aaataaggca ttcttatagt agaaaaggaa caccttatga
    16741 taatgctgga atcgaatctt tccatgcctc attgaaaaaa gaagaagtct acacgactag
    16801 ctactcagat tttgaagaag caaatcgagc gctatttagc tacattgaag gattttataa
    16861 ccgaaatcga atccatagct cgattcacta tctaacccca caggaatttg aagaattagc
    16921 aaaagaaaaa atggcttaac cgtctctact aaaatgtgtc caagatattg actcaaatcc
    16981 agtaatatca caataggaat gctgtttatt atattagcag cagtgctgat ttttatgata
    17041 gttatttggt tcttaaatga aagatctaaa gacaatcaac accgtaggga tatggagata
    17101 aaatcaatag agtcaataga ttatttatcg ataataacac taaaaaagcc acgttcctaa
    17161 acgtgactta cgctgaacga tatgtgttgt ttggtagacg aacaaatcgt tcattataaa
    17221 tatatgatgg tatcctaata tagtaagcgt ggctagcata cctttaatta ctttaccttt
    17281 tacagttacc atacacattt tttgagcaga aatcaagtaa ataaaaaaat attatatttc
    17341 acaacatgct aatagactca tatctagaac gcattaagta tttacctgtc tggttcgctg
    17401 agacttcttt tttaactttc ttcaattttc ttctttattc taaaaagcgt actgcgactg
    17461 aaaccggtta tagcttcaac ttccttatac gatttacctg aagtaagcag ttcatatgcg
    17521 tgcctcttct tcggtgtgat tgttgctttc ggacgtcctt ctctgaaatt aggattattc
    17581 tttcttgcaa acatttttcc ttcttgggta cgttccacaa tcatatcacg ttccatttct
    17641 gctacactca acaacgtccg tgtcaccatt cgccccatgg acgtattttc aatcgttcct
    17701 agattcaaaa ccttgatcgt tacgttatca tcgagtaaag gttcaatgat ctctaatgct
    17761 tctctcgtgt tacgagcaaa tcgatcaagc ttcgtaacaa ctatttcatc tccaggcttc
    17821 agaccagcca acagctgatt gaattttggc cgatcccgtt ttgtacccgt aaatttttct
    17881 gaaaaaatat tcttcttctc tacgccgttc tcaagcaact gttgaatttg ttcttttgta
    17941 ctttgagaag cggttgagac acgtgcatat ccatatttcg tcatttggtc cacctacttt
    18001 tacaaatcat ctatcgtata ctcagaatac ccttcataga aatagctgac atcgattcct
    18061 ttcatatata actcgcgatc gtcaatttta tcagtcagag cattggaaat tagatagcgt
    18121 atttccaaat cgtttatggg acttcgctcc attgcagaaa gatagtctgc tttatcgatt
    18181 aagttccaat ctacaacttt ttgcaattct tcctttaaaa tgagatccag ccaaattcta
    18241 gtacttcggc catttccttc tcgaaatggg tgagcgatat tcatctccac atatttcttg
    18301 ataatctctt caaaagaagt ttgtggcatc tgatcaatat gctgtaacga atgttctaaa
    18361 tacataactg gtgcaaatcg aaaatttcct tttgcaatat tgaccgatcg gatttttccg
    18421 gcaaaatcgt aaatatccga aaatagaaat tcatgaattt cagataaacc tttaaatgta
    18481 ccaacttcga tttcatttat ttttccacaa tcgtacaatt ctttcgcttt ttgtttactg
    18541 agtttttctt caaccttagc caactcaact tgattggtta atcccaattt attctctaaa
    18601 atcatgcgaa atctccttaa ctttatgaca taagtttatg aaacactctc aaccccatta
    18661 tatcgcgatt ttgtttatgt gtcaaaaact attaagtttc tgacccccga tagatttttc
    18721 aagtgtctct attttgcaca gtctagttgg ttctgttgca aagttttaaa taaagaataa
    18781 aatcccttac ggtatctatg atttaagctg ggattcccaa taataccttg atttcagtac
    18841 agaccgaaaa cccgaagaga gtgccttctt ttcgggtttt cttatataat cctcgaatgg
    18901 cttccatgcc tttaatcgtg gtagaggcag tgcgtaaact tcgatagaat ttattgcgtc
    18961 tctttactgg acgatggtct tgttcaatca aattattcag gtatttaatg gtacgatgtt
    19021 ctgtcccttg ataaaagccg tattctttta gtttcttaaa ggcacttgta atagaggggg
    19081 ctttatctgt gactacaacc ttcggttcat caaactgctt cactaaccgc ttaagaaaag
    19141 cataggctgc ttgtgtgtcc cgttttttac gtaaccaaat atccaaggtt aaaccatctg
    19201 catcgatggc tcgatacaaa taatgccatt ttcctttaat tttgatgtac gtttcatcca
    19261 ttttccatga ataaaaggat tttttatttt tctttttcca aatttgatag agtagtttgc
    19321 catattcttg cacccaacga taaatcgtcg tatgagaaac gttaatgcca cgatcatata
    19381 agatttcttg aacttcacga tagctaaggt tataacgaag atagtagccc acggctacaa
    19441 taatcacatc ctgctgaaat tgctttcctt taaaatgatt catcgtcatt cctcctgcta
    19501 tctttttcta ttattctacc ttatttgata gtagatttaa aactttgcaa cagaacc
//
