LOCUS       NC_013782               3071 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli plasmid pEC14-9, complete sequence.
ACCESSION   NC_013782
VERSION     NC_013782.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225396
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Pallecchi,L., Riccobono,E., Sennati,S., Mantella,A., Bartalesi,F.,
            Trigoso,C., Gotuzzo,E., Bartoloni,A. and Rossolini,G.M.
  TITLE     Characterization of small ColE-like plasmids mediating widespread
            dissemination of the qnrB19 gene in commensal enterobacteria
  JOURNAL   Antimicrob Agents Chemother 54 (2), 678-682 (2010)
   PUBMED   20008783
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Pallecchi,L., Riccobono,E., Sennati,S., Mantella,A., Bartoloni,A.
            and Rossolini,G.M.
  TITLE     Small qnrB19-harboring ColE1-like plasmid from commensal Escherichia
            coli in Bolivia
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3071)
  AUTHORS   Pallecchi,L., Riccobono,E., Sennati,S., Mantella,A., Bartoloni,A.
            and Rossolini,G.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2009) Molecular Biology Dept., University of
            Siena, V. Bracci, Siena 53100, Italy
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009953845.2-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 01:47:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GQ374157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="C14-9"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:562"
                     /plasmid="pEC14-9"
                     /geo_loc_name="Bolivia"
     gene            join(<3067..3071,1..151)
                     /locus_tag="HS640_RS00005"
                     /pseudo=""
     CDS             join(<3067..3071,1..151)
                     /locus_tag="HS640_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            245..889
                     /locus_tag="HS640_RS00010"
                     /old_locus_tag="pEC149_p1"
     CDS             245..889
                     /locus_tag="HS640_RS00010"
                     /old_locus_tag="pEC149_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(1980..2129)
                     /locus_tag="HS640_RS00015"
     CDS             complement(1980..2129)
                     /locus_tag="HS640_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162846302.1"
                     /translation="MPSEYDEQRGSTDTQLPGEYDLFDLMAKVTEEILHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     ARG             246..886
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(2165..2338)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          2317..2808
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 gctggatctg cgtgaattcc agcagcagca agagaaagac tttctacaaa ccagcttaca
       61 gcaggctaaa tttaaccaga aaaaagccgc tgaattactg ggtctgactt atcatcagct
      121 acgtgccttg ctgaagaaac accagatcta atacgcattt gcaggtgtca tactggcggg
      181 tttgacgcat aacgtcataa ggtttaccat ggcgtcatta ctgtataaaa acacaggcat
      241 agatatgact ctggcattag ttggcgaaaa aattgacaga aatcgcttca ccggtgagaa
      301 agttgaaaat agtacatttt ttaactgcga tttttcaggt gccgacctga gcggcactga
      361 atttatcggc tgccagttct atgatcgcga aagtcagaaa gggtgcaatt ttagtcgcgc
      421 aatgctgaaa gatgccattt tcaaaagctg tgatttatca atggcagatt tccgcaacgt
      481 cagtgccttg ggcattgaaa ttcgccactg ccgcgcacaa ggcgcagatt tccgcggtgc
      541 aagctttatg aatatgatca ccacgcgcac ctggttttgc agcgcatata tcactaatac
      601 taatctaagc tacgccaatt tttcgaaagt cgtgttggaa aagtgtgagc tgtgggaaaa
      661 ccgctggatg gggactcagg tactgggtgc gacgttcagt ggttcagatc tctccggcgg
      721 cgagttttcg actttcgact ggcgagcagc aaacttcaca cattgcgatc tgaccaattc
      781 ggagttaggt gacttagata ttcggggtgt tgatttacaa ggcgttaagt tagacagcta
      841 ccaggcatcg ttgctcatgg agcggcttgg catcgctgtg attggttagt tcagcgttta
      901 caccgggaga tgcgtttgac gatttacttt taaccagtac gactcctggc aagcattgtg
      961 aaacgttttc tccagtggac gaatatcctg acctaccgca tcaacgacat gccatctaat
     1021 gctgcattct ggacaggaat ttgatgaata tctaaccaat tcattttcgc gagaacaacg
     1081 aataaccact cccgaatcat gaagccatgt cttaacgaaa atatggctaa ctcatagcaa
     1141 aaaaaaagcc ccctaagggc ttttttttgt acctgacggt cagaccgatt aaacataaca
     1201 tcccttgcgt gcaccgcctg agaggctctg agggcgtttc tgacgggaaa ccggtaaaac
     1261 aggcaacaaa ccacccgaaa acccgccaga aacgcgcagg ctctgttttt acggcatgca
     1321 tgagtatgcg cgcaagtgca tagcgcggaa attcacacca ttcagccagg ggaagtcctg
     1381 tttccggcgt tcttcccatc cgaaaacggt gaacggggac gcaatttatt gcgtggccgt
     1441 gaagcgttgc cggcgcagcc ggaaaacccg ctgtcagcac gttcaggaag ggggtgatgt
     1501 acaaatggca cagaggatga aagtgacgta tgagctgaag gaatgatgta aaagtgatgt
     1561 ataaatgatg taaaagtgat gcaaaagtgt tgtaaaagtg atgtacaggc cgaaggagtg
     1621 ttgtaaaagt gttgtaaaag tgatgtatga actgaaggag cgatgtaatt ctgtagtcag
     1681 gaagaataac ccgttcatgc aggcatgaac gatacagtga ggaacgcttt tttttgtcac
     1741 gatctcccct taacctgcgt aaatcacccg ccatggttga ttatttttca acctcaaaat
     1801 ggtcatacac ctaacgcagc cgcgattttc atcgcaactg catgaattaa aagaaaaaaa
     1861 agtaatgttt cgttttcacg agtaataccc aggtattaaa aacccactgt aattacatga
     1921 aaataaagaa ataaaaggta ataccggcgt attaccacgt acgcgggcaa gcgaaaaagc
     1981 tataacgctt cccggccgac aggcggccca gaatccacag gggcatgcag gatctcttca
     2041 gtaaccttcg ccattaaatc aaaaaggtca tattcgccgg gaagctgcgt atcagtcgaa
     2101 ccccgttgtt catcatattc agatggcatg gggtgatatc cagtagcaga gaggttgtta
     2161 gcgggtttcg gggcgcagcc ctgaaccagt cacgtagcgc tagcggagtg tatactggct
     2221 gactatgttg gcacggatgt ggaaatcagt gaagtgcttc atgtagcagg agaaaaaagg
     2281 cggcaccagc gcgtcagcag gatatgtagt acaggatata ttccgcttcc tcgctcactg
     2341 actcgctgcg ctcggtcgtt cggctgcggc gagcggtgcc ggcttacagg cggggcggaa
     2401 atttcctggg agataccagg aaggagctta acagggaaga gataaggccg cggcgaagcc
     2461 gtttttccat aggctccgcc cccctgacaa acatcatgaa atctgacgct caaatcagtg
     2521 gtggcgaaac ccgatgcggc ttaaagatac caggcgtttc cccctggtgg ctccctcgtg
     2581 ctcccccgca ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgttta
     2641 tctcattcca cgcctgacac tcagttccgc gaaggcagtt cgctcctgga tggactgtat
     2701 gcacgaaccc cccgttcagt ccgactgctg cgccttatcc ggtaactatc atcttgagtc
     2761 caacccggaa agacacgact tatcgccact ggcagcagcc actggtaact gatttagagg
     2821 agttagtctt gaagttatgc gcctgttaag gctaaactga aagaacagat ttggttactg
     2881 cgctcctcgt aagccagtta ccacggttca aagagttgct gctctgagaa ccttcgaaaa
     2941 accgccttgc agggcggttt tttcgtttac agagcaagag attacgacgc cacagaaagg
     3001 atctcaagaa gatccttcac cgtaatggaa atctatccaa tccgagcaca taggatacac
     3061 atcgttataa c
//
