LOCUS       NC_013589               5159 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Escherichia coli plasmid pColE1-H22, complete sequence.
ACCESSION   NC_013589
VERSION     NC_013589.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224365
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Cursino,L., Smajs,D., Smarda,J., Nardi,R.M., Nicoli,J.R.,
            Chartone-Souza,E. and Nascimento,A.M.
  TITLE     Exoproducts of the Escherichia coli strain H22 inhibiting some
            enteric pathogens both in vitro and in vivo
  JOURNAL   J Appl Microbiol 100 (4), 821-829 (2006)
   PUBMED   16553738
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Cursino,L., Smajs,D., Smarda,J., Nardi,R.M.D., Nicoli,J.R.,
            Chartone-Souza,E. and Nascimento,A.M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2005) Biology, Masaryk University, Tomesova 12,
            Brno 602 00, Czech Republic
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009947555.2-RS_2024_06_23
            Annotation Date              :: 06/23/2024 04:18:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AY913943.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H22"
                     /db_xref="taxon:562"
                     /plasmid="pColE1-H22"
     gene            join(3650..5159,1..62)
                     /locus_tag="HS237_RS00005"
                     /old_locus_tag="pColE1H22_p1"
     CDS             join(3650..5159,1..62)
                     /locus_tag="HS237_RS00005"
                     /old_locus_tag="pColE1H22_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_012882752.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGSGGGGGTGGS
                     KSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEK
                     EQAETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSS
                     SIHARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARA
                     GDEMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKTAQTN
                     LLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKD
                     VLNKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDT
                     GDWKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTI
                     NEVLGI"
                     /name="colicin-like pore-forming protein"
     gene            448..585
                     /locus_tag="HS237_RS00015"
                     /old_locus_tag="pColE1H22_p3"
     CDS             448..585
                     /locus_tag="HS237_RS00015"
                     /old_locus_tag="pColE1H22_p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001329514.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISVQ
                     "
                     /name="colicin release lysis protein"
     gene            1144..1299
                     /locus_tag="HS237_RS00020"
     CDS             1144..1299
                     /locus_tag="HS237_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001490772.1"
                     /translation="MSVDFCDARQGGGAYGKTPAALASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(1427..1621)
                     /locus_tag="HS237_RS00025"
     CDS             complement(1427..1621)
                     /locus_tag="HS237_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041621.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1905..2228
                     /gene="mobA"
                     /locus_tag="HS237_RS00030"
     CDS             1905..2228
                     /gene="mobA"
                     /locus_tag="HS237_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(2453..2872)
                     /locus_tag="HS237_RS00035"
     CDS             complement(2453..2872)
                     /locus_tag="HS237_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2953..3390)
                     /locus_tag="HS237_RS00040"
     CDS             complement(2953..3390)
                     /locus_tag="HS237_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024247033.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLSGH"
                     /name="hypothetical protein"
     Typing          1657..1896
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(869..1398)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.776"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1906..2225
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.11e-62"
ORIGIN
        1 ttctatgtgc ctttattgat aagaataaac ttaatactat aaatgaggtg ttgggtattt
       61 aattattctt tattgatata aacagtccta gcaatccaaa tgggattgct aggaccaaac
      121 aaagtagatt gtatagcata aataggttta attttgccac gggggcatta tttaggtttt
      181 ttctttttct gaaaaaatct ttctttatga acttaaaaaa tatgtgttca atagcatatt
      241 ttgaatatgg gcatagaata gtgcttatca ctattgcgta tagcatctta tctgacgcta
      301 ggaaataata cccttcgttg ttttttgtta taaggtatat atatataagt gcgcagtata
      361 ggccaaacaa aatatttttt atgtagtatc ttaaactcat aaattaaacc tcgttatata
      421 ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgct
      481 ataaacctcc ttgttggatg tcaggctaac tatatacgtg atgttcaggg agggactgtg
      541 gctccatcat cttcttctaa attgacgggg atctctgttc agtagaaaag atcaaaggat
      601 cttcttgaga tccttttttt ctgcgcgtaa tctgctgcct gtaaacgaaa aaaccgcctg
      661 gggaggcggt ttgatcgaag gttaagtcag ttggggaact gcttaaccga ggtaactggc
      721 ttcagcagag cgcagatacc aaatactgtc cttccagtgt agccgtagtc gggccactac
      781 ttcaagaact ctgttgcacc atttgcgcca tcatcgctct gctaaccaag ttaccaacgg
      841 ctgctgccag tggcgctaag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
      901 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      961 cgacctacac cgagctgaga taccaacagc gtgagctatg agaaagcgcc acgcttcccg
     1021 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1081 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1141 ggcttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcct
     1201 gcggcgctgg cttcttccgg tgctttgctt tttgctcaca tgttctttcc ggctttatcc
     1261 cctgattctg tggataaccg tattaccgct tttgagtgag ctgacaccgc tcgccgcagt
     1321 cgaacgtccg agcgtagcga gtcagtgagc gaggaagcgg aagagcgcct tatgtgacat
     1381 tttctcctta cgctctgttg tgccgttcgg catcctgtcc tgagcgttat ctctctgtgc
     1441 tattttctac ttcaaagcgt gtctggatgc tgttctggag ttcttctgcg agttcgtgca
     1501 gcttctcaca catggtagcc tgttcgtcag catcgagtgc gtccagcttt tcgagcagcg
     1561 tcaggctttg gctttttatg aatcctgcca tgttgagtac ggcttgctgc tgcttgttca
     1621 tcttttcgtt ttctccgttc tgtctgtcat ctgcgttgtg tgattatatc gcgtaccact
     1681 tttcgactgt tttaccgccg gtattctgcc gtctgacgct ttgacgggta tttctgcctg
     1741 acaatactgt cactgccaaa aaactgccgt gcctttgtcg gtaattcgaa cttgctgaca
     1801 ggacaggatg tgtaattgtt ataccgcgca tacatgcacg ctattacaat taccctggtc
     1861 agggctttgc cccgacaccc atgtcagata cggagccatg ttttatgaca aaacgaagtg
     1921 gaagtaatac gcgcaggcgg gctatcagtc gccctgttcg tctgacggca gaagaagacc
     1981 aggaaatcag aaaaagggct gctgaatgcg gcaagaccgt ttccggtttt ttacgggcgg
     2041 cagctctcgg taagaaagtt aactcactga ctgatgatcg ggtactgaaa gaagttatga
     2101 gactgggggc gttacagaaa aaactcttta tcgacggcaa gcgtgtcggg gacagggagt
     2161 atgcggaggt gctgatcgct attacggagt atcaccgtgc cctgttatcc aggcttatgg
     2221 cagattagct tcccggagag aaaactgtcg aaaactgacg gtatgaacac cgtaagctcc
     2281 caaagtgatc accattcgct ttcatgcata gctatgcaga gagctgaaaa cgatcctgac
     2341 gcatccttcc tgtttttccg gggtaaaaca tctctttttg cggtgtctgc gtcagaatcg
     2401 cgttcagcgc gtttcagtgg tgcgtacaat taagggatta tggtaaatcc acttactgtc
     2461 tgccctcgta gccatcgaga taaaccgcag aaatcgtgtc agccagcagc ctggattttt
     2521 tcggggtaag gtctttgctg cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg
     2581 agtttaaaaa ttcctggctg tgaaactgtt gaatgcgttg ttgcacatca ctccgtgatt
     2641 ttcctgtcgc tttatcttta gctcccatat cccagaactg ccgaaaaaaa ggcaccatca
     2701 ttcgtgttgt gtcggctttt tgggtggaaa agttgggatc aaaaccgtca tcagaagcat
     2761 atacgtaatg ccgggtatgt tgttccacgc tgccctttgg ggtacaggct gtgagagcta
     2821 ctgttgctgt tatcatggcg ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct
     2881 ccctacctta aagtatttat tctcggtgag ttattatccg aagattatct tatcatcagt
     2941 ttctgagcgc gttcagtgac cggacagcct ggaccagaat ccccgtttct tttcctgctc
     3001 cagagcctgc ctgagctggg ctatctcatt ctgtagctgt ttccgttctg cttcctggcg
     3061 ccggcgctcc gtatcctggg cttgtttatc ctcaagcatc agcgtcaggc attgtttcag
     3121 ctcattcagt tcccggagaa tgcgttctgt ctgctgatca tgaggttttt ctgagtgtcc
     3181 ttgagtgtgc ctttctggtg tctcattccg ctttaattcg ccgtatgccc ggatcagttc
     3241 actggtttca aactcccgtc gtccgtcctg gctggtacgg taactgacaa gacctgagcg
     3301 catatcacgg taaaactgtg aacgcgatct tccagtcatt ttcagtgcgt cccggagtgt
     3361 gtgccatgcc ataaagttac agtgtcccat aggtgtctca tctcatggtt tcagtaaaac
     3421 ataatgaggt ctgagaacgg taatgtttgt gctggttttt gtggcatcgg gtggaaatag
     3481 cacgtggtgt gggtggctgt ttttttgatc gttttcacaa aaatgaaaat ccacagggtt
     3541 gacagggaaa atgcagcggc gtaattttaa actgtatata aaaccagtga ttatatatac
     3601 agtatattgc ttttaactat ttgtttttaa tgtcaaagag gattttataa tggaaacagc
     3661 tgtagcgtac tataaagatg gtgttcctta tgatgataag gggcaggtaa tcattactct
     3721 tttgaatggt aatccagacg ggagtggctc tggctctggc ggtggtggtg gaactggagg
     3781 tagcaaaagt gaaagttctg cagccattca tgccacagct aaatggtcta ctgctcaatt
     3841 gaagaaaacg caggcagaac aggctgcccg agcaaaagct gccgcagaag cacaggctaa
     3901 agcaaaagca aaccgggatg cgctgactca acatctgaag gatattgtga atgaggcgct
     3961 tcgccataat tccactcatc cggaggttat tgacctggct catgccaata atgcagcgat
     4021 gcaggcagaa gcagagcggt tgcgccttgc aaaagcagaa gaaaaagccc gtaaagaagc
     4081 ggaagctgcg gaaaaggctt ttcaggaagc agaacaacga cgtaaagaga tagagaagga
     4141 gcaggctgaa acagaacgcc aattgaaact ggctgaggat gaagagaaac gcctggcagc
     4201 attgagtgaa gaggcccggg ctgtggaggt ggcacaaaaa aatcttgctg ctgcacaatc
     4261 tgagctggcg aaagtggatg aagagattaa tacgctcaat acccgtttaa gctccagtat
     4321 tcatgcccgt gatgcagaaa cgaatacgct gtccggaaaa cgaaatgagt tggatcaggc
     4381 atctgctaaa tataaagaac tggatgaaag ggttaaactt ttatctccta gggcgaatga
     4441 tccgcttcag agtcgtcctt tttttgaggc caccagacta cgggcgagag ctggtgatga
     4501 gatggaggaa aaacaaaagc aggtaacagc atcagaaacg cgtcttaacc agattagctc
     4561 tgagataaat ggaatccaag aggctatttc tcaggctaat aataagcgaa gtacagcagt
     4621 ttcacgtatt catgatgctg aagataattt gaaaacagca cagactaatc tcctgaactc
     4681 gcagattaag gatgctgtgg atgcaacagt tagcttttat caaacgctat ctgaaaaata
     4741 tggtgaaaaa tattcaaaaa tggcacagga acttgctgat aaatctaaag gtaagaaaat
     4801 cagcaatgtg aatgaagctc tagctgcttt tgagaaatac aaggatgtat taaataagaa
     4861 attcagcaaa gcagaccgtg atgcaatttt caatgcactg gaggcggtta agtatgaaga
     4921 ctgggctaag catttagatc agtttgccaa gtacttgaag attacgggac atgtttcttt
     4981 tggatatgat gtggtatctg atatcctaaa aattaaggat acaggtgact ggaagccgct
     5041 atttcttaca ttagagaaga aagctgtaga tgctggagtt agttatgttg ttgttttact
     5101 ttttagtgtg cttgctggaa ctacattagg tatctggggg attgctattg ttacaggca
//
