LOCUS       NC_013452               4440 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Staphylococcus aureus subsp. aureus ED98 plasmid pT181, complete
            sequence.
ACCESSION   NC_013452
VERSION     NC_013452.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604165
            Assembly: GCF_000024585.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus subsp. aureus ED98
  ORGANISM  Staphylococcus aureus subsp. aureus ED98
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Lowder,B.V., Guinane,C.M., Ben Zakour,N.L., Weinert,L.A.,
            Conway-Morris,A., Cartwright,R.A., Simpson,A.J., Rambaut,A.,
            Nubel,U. and Fitzgerald,J.R.
  TITLE     Recent human-to-poultry host jump, adaptation, and pandemic spread
            of Staphylococcus aureus
  JOURNAL   Proc Natl Acad Sci U S A 106 (46), 19545-19550 (2009)
   PUBMED   19884497
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Lowder,B.V., Guinane,C.M., Ben Zakour,N.L. and Fitzgerald,J.Ross.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2009) The Roslin Institute and Centre for
            Infectious Diseases, Royal (Dick) School of Veterinary Studies,
            University of Edinburgh, The Chancellor's Building, New Royal
            Infirmary, 49 Little France Crescent, Edinburgh EH16 4SB, Scotland,
            UK
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000024585.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 01:05:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,856
            CDSs (total)                      :: 2,776
            Genes (coding)                    :: 2,692
            CDSs (with protein)               :: 2,692
            Genes (RNA)                       :: 80
            rRNAs                             :: 6, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 46 of 84
            Pseudo Genes (incomplete)         :: 38 of 84
            Pseudo Genes (internal stop)      :: 32 of 84
            Pseudo Genes (multiple problems)  :: 29 of 84
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP001783.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Staphylococcus aureus subsp. aureus ED98"
                     /mol_type="genomic DNA"
                     /strain="ED98"
                     /sub_species="aureus"
                     /db_xref="taxon:681288"
                     /plasmid="pT181"
     gene            44..1285
                     /gene="mobV"
                     /locus_tag="SAAV_RS14410"
                     /old_locus_tag="SAAV_b1"
     CDS             44..1285
                     /gene="mobV"
                     /locus_tag="SAAV_RS14410"
                     /old_locus_tag="SAAV_b1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            1812..2756
                     /locus_tag="SAAV_RS14415"
                     /old_locus_tag="SAAV_b3"
     CDS             1812..2756
                     /locus_tag="SAAV_RS14415"
                     /old_locus_tag="SAAV_b3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            2918..4297
                     /gene="tet(K)"
                     /locus_tag="SAAV_RS14420"
                     /old_locus_tag="SAAV_b4"
     ARG             2919..4294
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          1813..2756
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          45..1282
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attaaataca catgaaacct ttgtgcttag gagtgatttt tatatgtctt attccattgt
       61 tagagtttca aaagttaaat ctggaacaaa tacaacgggc atacaaaaac atgttcaaag
      121 agaaaataat aattatgaaa atgaagatat agaccatagt aaaacttact taaattatga
      181 tttggtaaat gctaataaac agaattttaa taacttgatt gatgaaaaaa tcgaacagaa
      241 ttatacaggc aaaagaaaaa ttagaacaga cgcgattaaa cacattgatg gtttaattac
      301 atcagacaat gatttctttg ataatcaaac gccagaagat acaaagcagt tttttgaata
      361 tgctaaagag tttttagaac aagaatacgg taaagataat ttattatatg caacagttca
      421 catggacgaa aaaacaccac atatgcatta tggcgttgtt ccaataactg atgatggtcg
      481 tttaagtgct aaagaagttg taggtaataa aaaagcttta acagcgtttc aagatagatt
      541 taatgagcat gttaaacaac gaggatatga tttagaacgt gggcaatcaa gacaagtaac
      601 aaatgctaaa catgagcaaa taagtcagta taaacaaaaa acagaatatc ataagcaaga
      661 atatgaacgt gagagccaaa aaacagacca tataaagcaa aagaacgata aattaatgca
      721 agagtaccaa aaatcgttaa atacgcttaa aaagcctata aatgttccgt atgagcaaga
      781 aactgaaaaa gtaggtggtt tatttagcaa agaaatacaa gaaactggaa atgttgtaat
      841 aagccaaaaa gatttcaatg aatttcagaa acagataaaa gctgctcaag atatttcgga
      901 agattacgag tatataaagt ctggtagagc cttagatgat aaagataagg aaatacgaga
      961 gaaagatgat ttattaaata aagcagttga gcgtattgaa aacgcagacg ataattttaa
     1021 ccaactttac gaaaatgcaa agccacttaa agagaatata gaaatagcgt taaagctttt
     1081 aaaaatctta ctaaaagagt tagaacgagt tttaggaaga aatacctttg cggaaagagt
     1141 taataagtta acagaagatg aaccaaaact aaatggttta gcaggaaact tagataaaaa
     1201 aatgaatcca gaattatatt cagaacagga acagcaacaa gaacaacaaa agaatcaaaa
     1261 acgagataga ggtatgcact tatagaacat gcatttatgc cgagaaaact tattggttgg
     1321 aatgggctat gtgttagcta acttgttagc gagttggttg gacttgaatt gggattaatc
     1381 ccaagaaagt accaactcaa caacacataa agccctgtag gttccgacca ataaggaaat
     1441 tggaataaag caataaaagg agttgaagaa atgaaattca gagaagcctt tgagaatttt
     1501 ataacaagta agtatgtact tggtgtttta gtagttttaa ctgtttacca gataatacaa
     1561 atgcttaaat aaaaaaagac ttgatctgat tagaccaagt cttttgatag tgttatatta
     1621 ataacaaaat aaaaaggagt cgctcacgcc ctgaccaatg tttgtgaacg acatcattca
     1681 aagaaaaaaa cactgagttg tttttataat cttgtatatt tagatattaa acgatattta
     1741 aatatacatc aagatatata tttgggtgag cgattcctta aacgaaattg agattaagga
     1801 gtcgattttt tatgtataaa aacaatcatg caaatcattc aaatcatttg gaaaatcacg
     1861 atttagacaa tttttctaaa accggctact ctaatagccg gttggacgca catactgtgt
     1921 gcatatctga tccaaaatta agttttgatg caatgacgat cgttggaaat ctcaaccgag
     1981 acaacgctca agccctttct aaatttatga gtgtagagcc ccaaataaga ctttgggata
     2041 ttcttcaaac aaagtttaaa gctaaagcac ttcaagaaaa agtttatatt gaatatgaca
     2101 aagtgaaagc agatagttgg gatagacgta atatgcgtat tgaatttaat ccaaacaaac
     2161 ttacacgaga tgaaatgatt tggttaaaac aaaatataat aagctacatg gaagatgacg
     2221 gttttacaag attagattta gcctttgatt ttgaagatga tttgagtgac tactatgcaa
     2281 tgtctgataa agcagttaag aaaactattt tttatggtcg taatggtaag ccagaaacaa
     2341 aatattttgg cgtgagagat agtaatagat ttattagaat ttataataaa aagcaagaac
     2401 gtaaagataa tgcagatgct gaagttatgt ctgaacattt atggcgtgta gaaatcgaac
     2461 ttaaaagaga tatggtggat tactggaatg attgctttag tgatttacat atcttgcaac
     2521 cagattggaa aactatccaa cgcactgcgg atagagcaat agtttttatg ttattgagtg
     2581 atgaagaaga atggggaaag cttcacagaa attctagaac aaaatataag aatttgataa
     2641 aagaaatttc gccagtcgat ttaacggact taatgaaatc gactttaaaa gcgaacgaaa
     2701 aacaattgca aaaacaaatc gatttttggc aacatgaatt taaattttgg aaatagtgta
     2761 catattaata ttactgaaca aaaatgatat atttaaacta ttctaattta ggaggatttt
     2821 tttatgaagt gtctatttaa aaatttgggg aatttatatg aggtgaaaga ataatttacc
     2881 cctataaact ttagtcacct caagtaaaga ggtaaaattg tttagtttat ataaaaaatt
     2941 taaaggtttg ttttatagcg ttttattttg gctttgtatt ctttcatttt ttagtgtatt
     3001 aaatgaaatg gttttaaatg tttctttacc tgatattgca aatcatttta atactactcc
     3061 tggaattaca aactgggtaa acactgcata tatgttaact ttttcgatag gaacagcagt
     3121 atatggaaaa ttatctgatt atataaatat aaaaaaattg ttaattattg gtattagttt
     3181 gagctgtctt ggttcattga ttgcttttat tggtcacaat cactttttta ttttgatttt
     3241 tggtaggtta gtacaaggag taggatctgc tgcattccct tcactgatta tggtggttgt
     3301 agctagaaat attacaagaa aaaaacaagg caaagccttt ggttttatag gatcaattgt
     3361 agctttaggt gaagggttag gtccttcaat agggggaata atagcacatt atattcattg
     3421 gtcttaccta cttatacttc ctatgattac aatagtaact ataccttttc ttattaaagt
     3481 aatggtacct ggtaaatcaa caaaaaatac attagatatc gtaggtattg ttttaatgtc
     3541 tataagtatt atatgtttta tgttatttac gacaaattat aattggactt ttttaatact
     3601 cttcacaatc ttttttgtga tttttattaa acatatttca agagtttcta acccttttat
     3661 taatcctaaa ctagggaaaa acattccgtt tatgcttggt ttgttttctg gtgggctaat
     3721 attttctata gtagctggtt ttatatcaat ggtgccttat atgatgaaaa ctatttatca
     3781 tgtaaatgta gcgacaatag gtaatagtgt tatttttcct ggaaccatga gtgttattgt
     3841 ttttggttat tttggtggtt ttttagtgga tagaaaagga tcattatttg tttttatttt
     3901 aggatcattg tctatctcta taagtttttt aactattgca ttttttgttg agtttagtat
     3961 gtggttgact acttttatgt ttatatttgt tatgggcgga ttatctttta ctaaaacagt
     4021 tatatcaaaa atagtatcaa gtagtctttc tgaagaagaa gttgcttctg gaatgagttt
     4081 gctaaatttc acaagttttt tatcagaggg aacaggtata gcaattgtag gaggtttatt
     4141 gtcactacaa ttgattaatc gtaaactagt tctggaattt ataaattatt cttctggagt
     4201 gtatagtaat attcttgtag ccatggctat ccttattatt ttatgttgtc ttttgacgat
     4261 tattgtattt aaacgttctg aaaagcagtt tgaatagtta tattatattt tggtttagaa
     4321 ctatgagtgg ctagcatttt gccactcatt ttttgcgtta gcaaaaacag gtttaagcct
     4381 cgcagagcac acgtattaac gacttattgt aaaataagtc tagtatgtta gacttaaact
//
