LOCUS       NC_013336               4498 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Staphylococcus aureus plasmid SAP060B, complete sequence.
ACCESSION   NC_013336
VERSION     NC_013336.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225399
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Summers,A.O., Shearer,J. and Wireman,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2009) Department of Microbiology, University of
            Georgia, 527 Biological Sciences Building, Athens, GA 30602, USA
  REMARK    Protein update by submitter
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Gill,J., Borman,J., Shetty,J., Hostetler,J., Durkin,S. and
            Montgomery,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2009) J. Craig Venter Institute, 9704 Medical
            Center Drive, Rockville, MD 20850, USA
REFERENCE   3  (bases 1 to 4498)
  AUTHORS   Summers,A.O., Shearer,J. and Wireman,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2009) Department of Microbiology, University of
            Georgia, 527 Biological Sciences Building, Athens, GA 30602, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 09/10/2023 04:00:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GQ900417.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="502A"
                     /db_xref="taxon:1280"
                     /plasmid="SAP060B"
                     /geo_loc_name="USA"
                     /identified_by="Jodi Lindsay, Department of Cellular and
                     Molecular Medicine, St. George's, University of London,
                     London, UK"
                     /note="MSSA; not from infection; collected pre-1960s"
     gene            join(3299..4498,1..42)
                     /gene="mobV"
                     /locus_tag="HHF13_RS00005"
                     /old_locus_tag="SAP060B_004"
     CDS             join(3299..4498,1..42)
                     /gene="mobV"
                     /locus_tag="HHF13_RS00005"
                     /old_locus_tag="SAP060B_004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     gene            229..408
                     /locus_tag="HHF13_RS00010"
                     /old_locus_tag="SAP060B_001"
     CDS             229..408
                     /locus_tag="HHF13_RS00010"
                     /old_locus_tag="SAP060B_001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000042035.1"
                     /translation="MSKNLDKGLVISTVLNVILCMLLILTMRVESLTVSVILAIITGVN
                     AVYLVAKVLKQKKT"
                     /name="hypothetical protein"
     gene            627..1571
                     /locus_tag="HHF13_RS00015"
                     /old_locus_tag="SAP060B_002"
     CDS             627..1571
                     /locus_tag="HHF13_RS00015"
                     /old_locus_tag="SAP060B_002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000042121.1"
                     /translation="MSKNNHTNHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            1733..3112
                     /gene="tet(K)"
                     /locus_tag="HHF13_RS00020"
                     /old_locus_tag="SAP060B_003"
     ARG             1734..3109
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          628..1571
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3300..4477
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="95.0"
                     /evalue="0.0"
ORIGIN
        1 caacaaaaga atcaaaaacg agatagaggt atgcacttat agaacatgca tttatgccga
       61 gaaaacttat tggttggaat gggctatgtg ttagctaact tgttagcgag ttggttggac
      121 ttgaattggg attaatccca agaaagtacc aactcaacaa cacataaagc cctgtaggtt
      181 ccgaccaata aggaaattgg aataaagcaa taaaaggagt tgaagaagat gagtaaaaat
      241 ttagataagg gattggttat atcaacagtt ttaaacgtta ttctttgcat gcttttaata
      301 ttaactatgc gagtagaaag tttaactgta tcagttatat tagcaataat cactggagta
      361 aatgcagtat atttagttgc taaggtttta aaacagaaaa agacctgatc tgattagacc
      421 aagtcttttg atagtgttat attaataaac aaataaaaaa gaagtcgctc actccctgac
      481 caaagtttgt gatagcgact tacccaaaga aaaaacacta agttgttttt ttatattttg
      541 tatatctaga tattaaacga tataagttta ttcttcaaga tatatattcg ggtgagcgac
      601 ttcttaaatt aaggagtcga ttttttatga gtaaaaataa tcatacaaat cattcaaatc
      661 acttagaaaa tcacgattta gacaattttt ctaaaaccgg ctactctaat agccggttgg
      721 acgcacatac tgtgtgcata tctgatccaa aattaagttt tgatgcaatg acgatcgttg
      781 gaaatctcaa ccgagacaac gctcaagccc tttctaaatt tatgagtgta gagccccaaa
      841 taagactttg ggatattctt caaacaaagt ttaaagctaa agcacttcaa gaaaaagttt
      901 atattgaata tgacaaagtg aaagcagata gttgggacag acgtaatatg cgtattgaat
      961 ttaatccaaa caaacttaca cgagatgaaa tgatttggtt aaaacaaaac attattgact
     1021 atatggaaga tgacggtttt acaagattag atttagcctt tgattttgaa gatgatttga
     1081 gtgactacta tgcaatgtct gataaagcag ttaagaaaac tattttttat ggtcgtaatg
     1141 gtaagccaga aacaaaatat tttggcgtga gagatagtaa tagatttatt agaatttata
     1201 ataaaaagca agaacgtaaa gataatgcag atgctgaagt tatgtctgaa catttatggc
     1261 gtgtagaaat cgaacttaaa agagatatgg tggattactg gaatgattgc tttagtgatt
     1321 tacatatctt gcaaccagat tggaaaacta tccaacgcac tgcggataga gcaatagttt
     1381 ttatgttatt gagtgatgaa gaagaatggg gaaagcttca cagaaattct agaacaaaat
     1441 ataagaattt gataaaagaa atttcgccag tcgatttaac ggacttaatg aaatcgactt
     1501 taaaagcgaa cgaaaaacaa ttgcaaaaac aaatcgattt ttggcaacat gaatttaaat
     1561 tttggaaata gtgtacatat taatattact gaacaaaaat gatatattta aactattcta
     1621 atttaggagg atttttttat gaagtgtcta tttaaaaatt tggggaattt atatgaggtg
     1681 aaagaataat ttacccctat aaactttagt cacctcaagt aaagaggtaa aattgtttag
     1741 tttatataaa aaatttaaag gtttgtttta tagcgtttta ttttggcttt gtattctttc
     1801 attttttagt gtattaaatg aaatggtttt aaatgtttct ttacctgata ttgcaaatca
     1861 ttttaatact actcctggaa ttacaaactg ggtaaacact gcatatatgt taactttttc
     1921 gataggaaca gcagtatatg gaaaattatc tgattatata aatataaaaa aattgttaat
     1981 tattggtatt agtttgagct gtcttggttc attgattgct tttattggtc acaatcactt
     2041 ttttattttg atttttggta ggttagtaca aggagtagga tctgctgcat tcccttcact
     2101 gattatggtg gttgtagcta gaaatattac aagaaaaaaa caaggcaaag cctttggttt
     2161 tataggatca attgtagctt taggtgaagg gttaggtcct tcaatagggg gaataatagc
     2221 acattatatt cattggtctt acctacttat acttcctatg attacaatag taactatacc
     2281 ttttcttatt aaagtaatgg tacctggtaa atcaacaaaa aatacattag atatcgtagg
     2341 tattgtttta atgtctataa gtattatatg ttttatgtta tttacgacaa attataattg
     2401 gactttttta atactcttca caatcttttt tgtgattttt attaaacata tttcaagagt
     2461 ttctaaccct tttattaatc ctaaactagg gaaaaacatt ccgtttatgc ttggtttgtt
     2521 ttctggtggg ctaatatttt ctatagtagc tggttttata tcaatggtgc cttatatgat
     2581 gaaaactatt tatcatgtaa atgtagcgac aataggtaat agtgttattt ttcctggaac
     2641 catgagtgtt attgtttttg gttattttgg tggtttttta gtggatagaa aaggatcatt
     2701 atttgttttt attttaggat cattgtctat ctctataagt tttttaacta ttgcattttt
     2761 tgttgagttt agtatgtggt tgactacttt tatgtttata tttgttatgg gcggattatc
     2821 ttttactaaa acagttatat caaaaatagt atcaagtagt ctttctgaag aagaagttgc
     2881 ttctggaatg agtttgctaa atttcacaag ttttttatca gagggaacag gtatagcaat
     2941 tgtaggaggt ttattgtcac tacaattgat taatcgtaaa ctagttctgg aatttataaa
     3001 ttattcttct ggagtgtata gtaatattct tgtagccatg gctatcctta ttattttatg
     3061 ttgtcttttg acgattattg tatttaaacg ttctgaaaag cagtttgaat agttatatta
     3121 tattttggtt tagaactatg agtggctagc attttgccac tcattttttg cgttagcaaa
     3181 aacaggttta agcctcgcag agcacacgta ttaacgactt attgtaaaat aagtctagtg
     3241 tgttagactt aaactattaa atacacatga aacctttgtg cttaggagtg atttttatat
     3301 gtcttattcc attgttagag tttcaaaagt taaatctgga acaaatacaa cgggcataca
     3361 aaaacatgtt caaagagaaa ataataatta tgaaaatgaa gatatagacc atagtaaaac
     3421 ttacttaaat tatgatttgg taaatgctaa taaacagaat tttaataact tgattgatga
     3481 aaaaatcgaa cagaattata caggcaaaag aaaaattaga acagacgcga ttaaacacat
     3541 tgatggttta attacatcag acaatgattt ctttgataat caaacgccag aagatacaaa
     3601 gcagtttttt gaatatgcta aagagttttt agaacaagaa tacggtaaag ataatttatt
     3661 atatgcaaca gttcacatgg acgaaaaaac accacatatg cattatggcg ttgttccaat
     3721 aactgatgat ggtcgtttaa gtgctaaaga agttgtaggt aataaaaaag ctttaacagc
     3781 gtttcaagat agatttaatg agcatgttaa acaacgagga tatgatttag aacgtgggca
     3841 atcaagacaa gtaacaaatg ctaaacatga gcaaataagt cagtataaac aaaaaacaga
     3901 atatcataag caagaatatg aacgtgagag ccaaaaaaca gaccatataa agcaaaagaa
     3961 cgataaatta atgcaagagt accaaaaatc gttaaatacg cttaaaaagc ctataaatgt
     4021 tccgtatgag caagaaactg aaaaagtagg tggtttattt agcaaagaaa tacaagaaac
     4081 tggaaatgtt gtaataagcc aaaaagattt caatgaattt cagaaacaga taaaagctgc
     4141 tcaagatatt tcggaagatt acgagtatat aaagtctggt agagccttag atgataaaga
     4201 taaggaaata cgagagaaag atgatttatt aaataaagca gttgagcgta ttgaaaacgc
     4261 agacgataat tttaaccaac tttacgaaaa tgcaaagcca cttaaagaga atatagaaat
     4321 agcgttaaag cttttaaaaa tcttactaaa agagttagaa cgagttttag gaagaaatac
     4381 ctttgcggaa agagttaata agttaacaga agatgaacca aaactaaatg gtttagcagg
     4441 aaacttagat aaaaaaatga atccagaatt atattcagaa caggaacagc aacaagaa
//
