LOCUS       NC_013314               2908 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Staphylococcus aureus plasmid SAP065A, complete sequence.
ACCESSION   NC_013314
VERSION     NC_013314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225459
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Summers,A.O., Shearer,J. and Wireman,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2009) Department of Microbiology, University of
            Georgia, 527 Biological Sciences Building, Athens, GA 30602, USA
  REMARK    Protein update by submitter
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Gill,J., Borman,J., Shetty,J., Hostetler,J., Durkin,S. and
            Montgomery,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2009) J. Craig Venter Institute, 9704 Medical
            Center Drive, Rockville, MD 20850, USA
REFERENCE   3  (bases 1 to 2908)
  AUTHORS   Summers,A.O., Shearer,J. and Wireman,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2009) Department of Microbiology, University of
            Georgia, 527 Biological Sciences Building, Athens, GA 30602, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/10/2023 04:02:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2
            CDSs (total)                      :: 2
            Genes (coding)                    :: 1
            CDSs (with protein)               :: 1
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GQ900420.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="EMRSA-10"
                     /db_xref="taxon:1280"
                     /plasmid="SAP065A"
                     /geo_loc_name="United Kingdom"
                     /collection_date="1987"
                     /identified_by="Jodi Lindsay, Department of Cellular and
                     Molecular Medicine, St. George's, University of London,
                     London, UK"
                     /note="HA-MRSA; clinical, hospital-acquired infection"
     gene            complement(83..802)
                     /gene="catA"
                     /locus_tag="HHF70_RS00005"
                     /old_locus_tag="SAP065A_001"
                     /pseudo=""
     ARG             complement(156..802)
                     /name="catA"
                     /gene="catA"
                     /gene_name="type A-9 chloramphenicol O-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1272..2195
                     /locus_tag="HHF70_RS00010"
                     /old_locus_tag="SAP065A_002"
     CDS             1272..2195
                     /locus_tag="HHF70_RS00010"
                     /old_locus_tag="SAP065A_002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_000417606.1"
                     /translation="MEKYTEKKQRNQVFQKFIKRHIGENQMDLVEDCNTFLSFVADKTL
                     EKQKLYKANSCKNRFCPVCAWRKARKDALGLSLMMQYIKQQEKKEFIFLTLTTPNVMSD
                     ELENEIKRYNNSFRKLIKRKKVGSVIKGYVRKLEITYNKKRDDYNPHFHVLIAVNKSYF
                     TDKRYYISQQEWLDLWRDVTGISEITQVQVQKIRQNNNKELYEMAKYSGKDSDYLINQK
                     VFDAFYKSLKGKQVLVYSGLFKEAKKKLKNGDLDYLKEIDPTEYIYQIFYIWKQKEYLA
                     SELYDLTEQEKREINHKMIDEIEEEQ"
                     /name="protein rep"
     ARG             complement(153..802)
                     /name="Streptococcus suis chloramphenicol
                     acetyltransferase"
                     /gene="Streptococcus suis chloramphenicol
                     acetyltransferase"
                     /gene_name="chloramphenicol acetyltransferase (CAT)"
                     /tool="rgi"
                     /drug_class="phenicol antibiotic"
                     /identity="94.91"
                     /coverage="100.0"
     Typing          1261..2195
                     /name="rep_cluster_1230"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attggaataa agaagcgaaa aaggagaagt cggttcagaa aaagaaggat atggatctgg
       61 agctgtaata taaaaacctt cttcaactaa cggggcaggt tagtgacatt agaaaaccga
      121 ctgtaaaaag tacagtcggc attatctcat attataaaag ccagtcatta ggcctatctg
      181 acaattcctg aatagagttc ataaacaatc ctgcatgata accatcacaa acagaatgat
      241 gtacctgtaa agatagcggt aaatatattg aattaccttt attaatgaat tttcctgctg
      301 taataatggg tagaaggtaa ttactattat tattgatatt taagttaaac ccagtaaatg
      361 aagtccatgg aataatagaa agagaaaaag cattttcagg tataggtgtt ttgggaaaca
      421 atttccccga accattatat ttctctacat cagaaaggta taaatcataa aactctttga
      481 agtcattctt tacaggagtc caaataccag agaatgtttt agatacacca tcaaaaattg
      541 tataaagtgg ctctaactta tcccaataac ctaactctcc gtcgctattg taaccagttc
      601 taaaagctgt atttgagttt atcacccttg tcactaagaa aataaatgca gggtaaaatt
      661 tatatccttc ttgttttatg tttcggtata aaacactaat atcaatttct gtggttatac
      721 taaaagtcgt ttgttggttc aaataatgat taaatatctc ttttctcttc caattgtcta
      781 aatcaatttt attaaagttc atttgatatg cctcctaaat ttttatctaa agtgaattta
      841 ggaggcttac ttgtctgctt tcttcattag aatcaatcct tttttaaaag tcaatattac
      901 tgtaacataa atatatattt taaaaatatc ccactttatc caattttcgt ttgttgaact
      961 aatgggtgct ttagttgaag aataaaagac cacattaaaa aatgtggtct tttgtgtttt
     1021 tttaaaggat ttgagcgtag cgaaaaatcc ttttctttct tatcttgata ataagggtaa
     1081 ctattgccgg cgaggctagt tacccttaag ttattggtat gactggtttt aagcgcaaaa
     1141 aaagttgctt tttcgtacct attaatgtat cgttttaaat gactagtaaa aaacatacat
     1201 agaaagggga aaaagcaact ttttttattg tcatagtttg tgaaaactaa gttgttttta
     1261 tgtgttataa catggaaaag tatactgaga aaaaacaaag aaatcaagta tttcagaaat
     1321 ttattaaacg tcatattgga gagaatcaaa tggatttagt tgaagattgc aatacatttc
     1381 tgtcttttgt agctgataaa actttagaaa aacagaaatt atataaagct aattcttgta
     1441 aaaatcgatt ttgtcctgtc tgtgcttgga gaaaagctag aaaagatgca ttgggtttat
     1501 ctttgatgat gcaatatatt aagcagcaag agaaaaagga gtttatcttt ttaactttga
     1561 ctacacctaa tgtaatgagt gatgaattag aaaatgaaat aaaacgttat aataattctt
     1621 ttagaaaact tataaagaga aaaaaagtag gtagtgttat aaagggatat gttcgtaagt
     1681 tagagattac atataataaa aaaagagatg attataatcc tcattttcat gtgttaattg
     1741 cagtaaataa atcgtatttc acagataaaa gatattatat tagccaacaa gaatggttag
     1801 atttatggcg tgatgtaacg ggcatttcag aaataacaca agttcaagtt caaaaaataa
     1861 gacaaaataa taataaagaa ttatatgaaa tggctaagta ttctggtaaa gatagtgatt
     1921 atttaataaa tcaaaaagtc tttgatgcat tttataaatc acttaaaggt aaacaggtat
     1981 tagtttattc aggattattt aaagaggcta aaaagaaatt aaaaaatggg gatttagatt
     2041 acttaaaaga aattgatcca accgaatata tctatcaaat tttttatatt tggaaacaaa
     2101 aagagtattt agctagtgaa ctttatgact taacagaaca agaaaaaaga gaaattaatc
     2161 acaaaatgat agacgaaatc gaggaagaac aataacaaaa tataagtgct aacagctgac
     2221 ctcccgataa caccatgtag ttattgggag gtcagctgtt gaattatgca cgagtatttt
     2281 aaaagttatt gtgatgacga cgataaacga ttatcaaaag tataatgtta aaatgcttta
     2341 ttatactaac gttatataaa cattatactt tcgttataca aattttaacc ctgttaggaa
     2401 ctataaaaaa tcatgaaaat tttaatttgc atgtaactgg gcagtgtctt aaaaaatcga
     2461 cactgaattt gctcaaattt ttgtttgtag aattagaata tatttatttg gctcatattt
     2521 gctttttaaa agcttctgta ggtttttagg cataaaacta tatgatttac ccctaaatct
     2581 ttaaaatgcc ccttaaaatt caaaataaag gcatttaaaa tttaaatatt tcttgtgata
     2641 aagtttgtta aaaaggagtg gttttatgac tgttatgtgg ttatcgatta taggtatgtg
     2701 gttttgtatt ggaatggcat tttttgctat caaggttatt aaaaataaaa attagaccac
     2761 gcatttatgc cgagaaaatt tattgtgcgt tgagaagaac ccttaactaa acttgcagac
     2821 gaatgtcggc atagcgtgag ctattaagcc gaccattcga caagttttgg gattgttaag
     2881 ggttccgagg ctcaacgtca ataaagca
//
