LOCUS       NC_013312               4439 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Staphylococcus aureus plasmid SAP095B, complete sequence.
ACCESSION   NC_013312
VERSION     NC_013312.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225434
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Summers,A.O., Shearer,J. and Wireman,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2009) Department of Microbiology, University of
            Georgia, 527 Biological Sciences Building, Athens, GA 30602, USA
  REMARK    Protein update by submitter
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Gill,J., Borman,J., Shetty,J., Hostetler,J., Durkin,S. and
            Montgomery,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2009) J. Craig Venter Institute, 9704 Medical
            Center Drive, Rockville, MD 20850, USA
REFERENCE   3  (bases 1 to 4439)
  AUTHORS   Summers,A.O., Shearer,J. and Wireman,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2009) Department of Microbiology, University of
            Georgia, 527 Biological Sciences Building, Athens, GA 30602, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/10/2023 04:20:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3
            CDSs (total)                      :: 3
            Genes (coding)                    :: 2
            CDSs (with protein)               :: 2
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GQ900445.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="SK1373"
                     /db_xref="taxon:1280"
                     /plasmid="SAP095B"
                     /geo_loc_name="Australia: Melbourne"
                     /collection_date="1960"
                     /identified_by="Ron Skurray, Neville Firth, Slade Jensen:
                     School of Biological Sciences, University of Sydney,
                     Sydney, AU"
                     /note="Tc; human clinical isolate"
     gene            complement(40..984)
                     /locus_tag="HHF45_RS00005"
                     /old_locus_tag="SAP095B_001"
     CDS             complement(40..984)
                     /locus_tag="HHF45_RS00005"
                     /old_locus_tag="SAP095B_001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(1511..2752)
                     /gene="mobV"
                     /locus_tag="HHF45_RS00010"
                     /pseudo=""
     CDS             complement(1511..2752)
                     /gene="mobV"
                     /locus_tag="HHF45_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            complement(2938..4317)
                     /gene="tet(K)"
                     /locus_tag="HHF45_RS00015"
                     /old_locus_tag="SAP095B_003"
     ARG             complement(2942..4317)
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(41..984)
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1515..2752)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.758"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaatatatc atttttgttc agtaatatta atatgtacac tatttccaaa atttaaattc
       61 atgttgccaa aaatcgattt gtttttgcaa ttgtttttcg ttcgctttta aagtcgattt
      121 cattaagtcc gttaaatcga ctggcgaaat ttcttttatc aaattcttat attttgttct
      181 agaatttctg tgaagctttc cccattcttc ttcatcactc aataacataa aaactattgc
      241 tctatccgca gtgcgttgga tagttttcca atctggttgc aagatatgta aatcactaaa
      301 gcaatcattc cagtaatcca ccatatctct tttaagttcg atttctacac gccataaatg
      361 ttcagacata acttcagcat ctgcattatc tttacgttct tgctttttat tataaattct
      421 aataaatcta ttactatctc tcacgccaaa atattttgtt tctggcttac cattacgacc
      481 ataaaaaata gttttcttaa ctgctttatc agacattgca tagtagtcac tcaaatcatc
      541 ttcaaaatca aaggctaaat ctaatcttgt aaaaccgtca tcttccatgt agcttattat
      601 attttgtttt aaccaaatca tttcatctcg tgtaagtttg tttggattaa attcaatacg
      661 catattacgt ctatcccaac tatctgcttt cactttgtca tattcaatat aaactttttc
      721 ttgaagtgct ttagctttaa actttgtttg aagaatatcc caaagtctta tttggggctc
      781 tacactcata aatttagaaa gggcttgagc gttgtctcgg ttgagatttc caacgatcgt
      841 cattgcatca aaacttaatt ttggatcaga tatgcacaca gtatgtgcgt ccaaccggct
      901 attagagtag ccggttttag aaaaattgtc taaatcgtga ttttccaaat gatttgaatg
      961 atttgcatga ttgtttttat acataaaaaa tcgactcctt aatctcaatt tcgtttaagg
     1021 aatcgctcac ccaaatatat atcttgatgt atatttaaat atcgtttaat atctaaatat
     1081 acaagattat aaaaataact cagtgttttt ttctttgaat gatgtcgttc acaaactttg
     1141 gtcagggcgt gagcgactcc tttttatttt gttattaata taacactatc aaaagacttg
     1201 gtctaatcag atcaagtctt tttttattta agcatttgta ttatctggta aacagttaaa
     1261 actactaaaa caccaagtac atacttactt gttataaaat tctcaaaggc ttctctgaat
     1321 ttcatttctt caactccttt tattgcttta ttccaatttc cttattggtc ggaacctaca
     1381 gggctttatg tgttgttgag ttggtacttt cttgggatta atcccaattc aagtccaacc
     1441 aactcgctaa caagttagct aacacatagc ccattccaac caataagttt tctcggcata
     1501 aatgcatgtt ctataagtgc atacctctat ctcgtttttg attcttttgt tgttcttgtt
     1561 gctgttcctg ttctgaatat aattctggat tcattttttt atctaagttt cctgctaaac
     1621 catttagttt tggttcatct tctgttaact tattaactct ttccgcaaag gtatttcttc
     1681 ctaaaactcg ttctaactct tttagtaaga tttttaaaag ctttaacgct atttctatat
     1741 tctctttaag tggctttgca ttttcgtaaa gttggttaaa attatcgtct gcgttttcaa
     1801 tacgctcaac tgctttattt aataaatcat ctttctctcg tatttcctta tctttatcat
     1861 ctaaggctct accagacttt atatactcgt aatcttccga aatatcttga gcagctttta
     1921 tctgtttctg aaattcattg aaatcttttt ggcttattac aacatttcca gtttcttata
     1981 tttctttgct aaataaacca cctacttttt cagtttcttg ctcatacgga acatttatag
     2041 gctttttaag cgtatttaac gatttttggt actcttgcat taatttatcg ttcttttgct
     2101 ttatatggtc tgttttttgg ctctcacgtt catattcttg cttatgatat tctgtttttt
     2161 gtttatactg acttatttgc tcatgtttag catttgttac ttgtcttgat tgcccacgtt
     2221 ctaaatcata tcctcgttgt ttaacatgct cattaaatct atcttgaaac gctgttaaag
     2281 cttttttatt acctacaact tctttagcac ttaaacgacc atcatcagtt attggaacaa
     2341 cgccataatg catatgtggt gttttttcgt ccatgtgaac tgttgcatat aataaattat
     2401 ctttaccgta ttcttgttct aaaaactctt tagcatattc aaaaaactgc tttgtatctt
     2461 ctggcgtttg attatcaaag aaatcattgt ctgatgtaat taaaccatca atgtgtttaa
     2521 tcgcgtctgt tctaattttt cttttgcctg tataattctg ttcgattttt tcatcaatca
     2581 agttattaaa attctgttta ttagcattta ccaaatcata atttaagtaa gttttactat
     2641 ggtctatatc ttcattttca taattattat tttctctttg aacatgtttt tgtatgcccg
     2701 ttgtatttgt tccagattta acttttgaaa ctctaacaat ggaataagac atataaaaat
     2761 cactcctaag cacaaaggtt tcatgtgtat ttaatagttt aagtctaaca cactagactt
     2821 atttttaata agtcgttaat acgtgtgctc tgcgaggctt aaacctgttt ttgctaacgc
     2881 aaaaaatgag tggcaaaatg ctagccactc atagttctaa accaaaatat aatataacta
     2941 ttcaaactgc ttttcagaac gtttaaatac aataatcgtc aaaagacaac ataaaataat
     3001 aaggatagcc atggctacaa gaatattact atacactcca gaagaataat ttataaattc
     3061 cagaactagt ttacgattaa tcaattgtag tgacaataaa cctcctacaa ttgctatacc
     3121 tgttccctct gataaaaaac ttgtgaaatt tagcaaactc attccagaag caacttcttc
     3181 ttcagaaaga ctacttgata ctatttttga tataactgtt ttagtaaaag ataatccgcc
     3241 cataacaaat ataaacataa aagtagtcaa ccacatacta aactcaacaa aaaatgcaat
     3301 agttaaaaaa cttatagaga tagacaatga tcctaaaata aaaacaaata atgatccttt
     3361 tctatccact aaaaaaccac caaaataacc aaaaacaata acactcatgg ttccaggaaa
     3421 aataacacta ttacctattg tcgctacatt tacatgataa atagttttca tcatataagg
     3481 caccattgat ataaaaccag ctactataga aaatattagc ccaccagaaa acaaaccaag
     3541 cataaacgga atgtttttcc ctagtttagg attaataaaa gggttagaaa ctcttgaaat
     3601 atgtttaata aaaatcacaa aaaagattgt gaagagtatt aaaaaagtcc aattataatt
     3661 tgtcgtaaat aacataaaac atataatact tatagacatt aaaacaatac ctacgatatc
     3721 taatgtattt tttgttgatt taccaggtac cattacttta ataagaaaag gtatagttac
     3781 tattgtaatc ataggaagta taagtaggta agaccaatga atataatgtg ctattattcc
     3841 ccctattgaa ggacctaacc cttcacctaa agctacaatt gatcctataa aaccaaaggc
     3901 tttgccttgt ttttttcttg taatatttct agctacaacc accataatca gtgaagggaa
     3961 tgcagcagat cctactcctt gtactaacct accaaaaatc aaaataaaaa agtgattgtg
     4021 accaataaaa gcaatcaatg aaccaagaca gctcaaacta ataccaataa ttaacaattt
     4081 ttttatattt atataatcag ataattttcc atatactgct gttcctatcg aaaaagttaa
     4141 catatatgca gtgtttaccc agtttgtaat tccaggagta gtattaaaat gatttgcaat
     4201 atcaggtaaa gaaacattta aaaccatttc atttaataca ctaaaaaatg aaagaataca
     4261 aagccaaaat aaaacgctat aaaacaaacc tttaaatttt ttatataaac taaacaattt
     4321 tacctcttta cttgaggtga ctaaagttta taggggtaaa ttattctttc acctcatata
     4381 aattccccaa atttttaaat agacacttca taaaaaaatc ctcctaaatt agaatagtt
//
