LOCUS       NC_013056               8771 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter calcoaceticus plasmid pMMCU1, complete sequence.
ACCESSION   NC_013056
VERSION     NC_013056.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225339
KEYWORDS    RefSeq.
SOURCE      Acinetobacter calcoaceticus
  ORGANISM  Acinetobacter calcoaceticus
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Merino,M., Acosta,J., Poza,M., Sanz,F., Beceiro,A., Chaves,F. and
            Bou,G.
  TITLE     OXA-24 carbapenemase gene flanked by XerC/XerD-like recombination
            sites in different plasmids from different Acinetobacter species
            isolated during a nosocomial outbreak
  JOURNAL   Antimicrob Agents Chemother 54 (6), 2724-2727 (2010)
   PUBMED   20385865
REFERENCE   2  (bases 1 to 8771)
  AUTHORS   Merino,M., Poza,M. and Bou,G.
  TITLE     First description of a carbapenem-hydrolyzing oxacillinase OXA 24
            from a plasmid in a clinical strain of Acinetobacter calcoaceticus
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8771)
  AUTHORS   Merino,M., Poza,M. and Bou,G.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2009) Microbiology, Complejo Hospitalario
            Universitario- Instituto de Investigacion Biomedica, Xubias de
            Arriba s/n, A Coruna 15006, Spain
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 11/28/2023 04:28:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 13
            CDSs (total)                 :: 13
            Genes (coding)               :: 13
            CDSs (with protein)          :: 13
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            GQ342610.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Acinetobacter calcoaceticus"
                     /mol_type="genomic DNA"
                     /strain="Acal H12O-07"
                     /db_xref="taxon:471"
                     /plasmid="pMMCU1"
     gene            74..631
                     /locus_tag="HPE72_RS00005"
                     /old_locus_tag="PMMCU1p1"
     CDS             74..631
                     /locus_tag="HPE72_RS00005"
                     /old_locus_tag="PMMCU1p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017398013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_012780177.1"
                     /translation="MKSLSVIELSNLYGMTRQAVYKQINKGNLSKNSDGKIDLSEAIRV
                     FGEPSKYVNSLQTTETRKLSEVHLLEQQVHMLQKQLEQAHEREKFQRDELKAKNDQLHV
                     KDEQIEAMQRLLEAPKTNMTTFADQKPSQDIATDPRPEPEPKHDGLTTPVEIENKRIPV
                     PEQVELEQPKRGWLSRFFLPNG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(826..1101)
                     /locus_tag="HPE72_RS00010"
     CDS             complement(826..1101)
                     /locus_tag="HPE72_RS00010"
                     /inference="COORDINATES: protein motif:HMM:NF015807.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="WP_075911389.1"
                     /translation="MIKKYTQVNFRLPLDLKEEIEQSASLTGNSITAEIVERLRNSFEY
                     DNLMLDNIELQSELINLESEKLDLLLEYQDKLCKIQDQILEELKKK"
                     /name="Arc family DNA-binding protein"
     gene            1195..1593
                     /locus_tag="HPE72_RS00015"
     CDS             1195..1593
                     /locus_tag="HPE72_RS00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_171264335.1"
                     /translation="MSNIIEFKRNERRFINQKPISPVELVTNILTPQEVDIKSLIKETK
                     KILREKGLKFSTSHQDLEHLKGNNLILFSKKKCGFILTTKLACSTNSSWEIYSISIDKT
                     TKALDIVNKIISRLGDNVHGSSREEAIS"
                     /name="hypothetical protein"
     gene            1562..1780
                     /locus_tag="HPE72_RS00020"
     CDS             1562..1780
                     /locus_tag="HPE72_RS00020"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_075911387.1"
                     /translation="MAAAEKKQSANPAMEAAKEAANIKIDGFEQGVNTYNAIGSQLLRE
                     KRDPSVINKTRENRRNIRKAREAIKNL"
                     /name="hypothetical protein"
     gene            complement(1869..2294)
                     /locus_tag="HPE72_RS00060"
     CDS             complement(1869..2294)
                     /locus_tag="HPE72_RS00060"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227558792.1"
                     /translation="MIARYAFKDIEIINDSIKITGKQIKGASILANYQRNGVSSFIYKY
                     LLKKYGVLVCDNYQTYKGHMLWVLSIVKLSVIRIYDLTKKEFIGTFDKVSPCLIKPWSV
                     PYNFPSDKEKFLRLDACVYTELEFHSLVLVTFAEDMF"
                     /name="hypothetical protein"
     gene            complement(2321..2602)
                     /locus_tag="HPE72_RS00065"
     CDS             complement(2321..2602)
                     /locus_tag="HPE72_RS00065"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227558793.1"
                     /translation="MTPPHILLEENYNKPLIPLPVYFSYENILESKYFYNQREGGIFCL
                     KELQASEGSEYSGCIELFYCEFNNEYALDGVCAVADEYLDDMTLLIKP"
                     /name="hypothetical protein"
     gene            complement(2621..2941)
                     /locus_tag="HPE72_RS00030"
                     /old_locus_tag="PMMCU1p2"
     CDS             complement(2621..2941)
                     /locus_tag="HPE72_RS00030"
                     /old_locus_tag="PMMCU1p2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012780178.1"
                     /translation="MFTRTVQTLKNSTDLVQRFTMPNIRQTFELRRFSEKEKNKQYILI
                     FKDIILNKKDWDDVKVVAEIQERNNSLRFSIKASKQYPELTSYEKMLEAKINDIIKPTL
                     VA"
                     /name="hypothetical protein"
     gene            3092..3502
                     /locus_tag="HPE72_RS00035"
     CDS             3092..3502
                     /locus_tag="HPE72_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005207185.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_075911385.1"
                     /translation="MLNFELKEKWGENSLILGFTQIPTTLIYAQKELGLSSIEINILLN
                     LLTHWWKKEEFPYPSQAGIAYRMGVSTRTVQRTLAGLETKGFITRNKTSRDNSKYKGRS
                     IYDLSPLVKILEEKAPDLDIVKKIKKNKRLAK"
                     /name="helix-turn-helix domain-containing protein"
     gene            4217..5044
                     /locus_tag="HPE72_RS00040"
                     /old_locus_tag="PMMCU1p3"
     CDS             4217..5044
                     /locus_tag="HPE72_RS00040"
                     /old_locus_tag="PMMCU1p3"
                     /EC_number="3.5.2.6"
                     /function="hydrolysis of beta-lactams"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
                     /protein_id="WP_012754353.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MGVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
     gene            complement(5113..5871)
                     /locus_tag="HPE72_RS00045"
                     /old_locus_tag="PMMCU1p4"
     CDS             complement(5113..5871)
                     /locus_tag="HPE72_RS00045"
                     /old_locus_tag="PMMCU1p4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015060804.1"
                     /translation="MALDKSKKRGRPAQLLQIAELHAFVDYLSQKKDRSDLQNDVIAML
                     RIEKFNFESLSEAEQILVKEALKPYREHMKLNLLFDEVSVKYPQTAYERKFVQLFEAYR
                     DNALSGADFNILKTMATRYLSFKAHKLELSDLELYLSQIQKKEASKKRTAENHRKFELG
                     GAVLAAFKELGIDISQDTPEQMKNRIQNTKKFHDDVMKSKIYQEVKSYKNGYFERNKLF
                     HQVLEGLNTWKKDGELLSVIEIKKALVKNQ"
                     /name="hypothetical protein"
     gene            6174..7343
                     /locus_tag="HPE72_RS00050"
                     /old_locus_tag="PMMCU1p5"
     CDS             6174..7343
                     /locus_tag="HPE72_RS00050"
                     /old_locus_tag="PMMCU1p5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_012780180.1"
                     /translation="MASYHFSVKSKNKGYALSHYLYISRLMQYEGIRKKSAEVLEHVEP
                     GQCMPSWVKDPVEFWQAADTFERANAKAYMEYEIALPNEFTVEQRKTLVETFVDKTIAT
                     HHYPHSYAIHNVKSRLSGEEQPHCHLMFSLKADDGIERSPEQYFKRYNAKEPSKGGAKK
                     IQLQDGHADYTTYLLHIRKAWENHLNDALAEHSPTVTYQIDGQKIQIKNQVSADNYEKY
                     NEIHGTLYLPEPKLGTGKQNETVEYLAEIQKIRQHNAKERELEQRQQYFDQQHEYYYSC
                     SNEPFSSPEVMHYLNHLIKKASNKKELGHDIDEIIQTQQPILHHEKWDIDTINFNLNNA
                     RLYPEPDMEFKVRSVQPKPVIEPTSPKSNLKYNPHSLNRNEMIYDGPGF"
                     /name="MobA/MobL family protein"
     gene            7465..7599
                     /locus_tag="HPE72_RS00070"
     CDS             7465..7599
                     /locus_tag="HPE72_RS00070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_264820919.1"
                     /translation="MLKLASRPSEASKKLYERSEFRVAFALAFTFSQIHEGINRKIAP"
                     /name="hypothetical protein"
     gene            7881..8768
                     /gene="repM"
                     /locus_tag="HPE72_RS00055"
                     /old_locus_tag="PMMCU1p6"
     CDS             7881..8768
                     /gene="repM"
                     /locus_tag="HPE72_RS00055"
                     /old_locus_tag="PMMCU1p6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360254.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_012780181.1"
                     /translation="MDKNQIVKSNQVIEASYQLSAVEQRIVLAAISRIPKSQPITDDEL
                     YPVSVNELQLLGVHEKTAYRDLKEGINRLYERSINLSIDDKSIKMRWVQEIQFLDSQSI
                     IGIRFSKPILPFISNLSREFTKYALSDIAGINSGYGIRIYELLVQYRQIGKREISVENL
                     RSMLELGKKYPLFADFKKRVIDTAVDQINECSPLSVSYEQKKTGRKVTHIQFSFKEKSK
                     SINHLNEPEKFYKLTDAQVHMFGNQLSRLHELSHLATQGESYDDLAATIKEMLRDPIQQ
                     KQFIPHLRNLGFKG"
                     /name="repM"
     ARG             4218..5041
                     /name="blaOXA-24"
                     /gene="blaOXA-24"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7882..8768
                     /name="rep_cluster_1364"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6253..7340
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.143"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttgattgtgt atgtagcggt tgactgtttc ttgatgtatc ttagttcaca taatagacaa
       61 cctaaattac aacatgaaat cactatctgt tatcgagctt tcaaacctct atggaatgac
      121 tcgtcaagcc gtttataaac aaataaataa aggtaattta agtaagaata gtgatggtaa
      181 aattgatctt tctgaggcca ttcgtgtctt tggtgagcca tctaaatatg taaacagttt
      241 acagacaacc gaaacacgaa agttgtcaga agttcactta cttgaacagc aggttcacat
      301 gcttcaaaaa cagcttgaac aagctcatga gcgtgagaag tttcaacgag atgaattaaa
      361 agctaagaat gatcagttac atgttaagga tgaacaaatt gaggctatgc aacgcttatt
      421 agaagcccca aaaaccaata tgactacgtt tgccgatcag aaacctagcc aggatatagc
      481 aacggatcct cgaccagaac cagagccgaa acatgacgga ttgactactc cagttgaaat
      541 agaaaataag cggattccag tgccggagca ggttgaactg gagcagccga aaagagggtg
      601 gttaagccgg tttttcttac ctaatgggta aaaggcttgt ttaggtgtac taatcaaagt
      661 tgtgaaacat tactcaaata ataggcgtgg aacgtgaata atcatgaaaa agccgttcta
      721 gtgcgctgaa ctctagaacg gctttgacta aactattgat gcttataaga aaatttcaag
      781 catttcgcat aaggcgtatt atgttaattt tagaaaacct taaatctatt ttttctttaa
      841 ttcctctaaa atttggtctt gtatcttaca taatttatct tggtattcta aaagtaaatc
      901 taatttctca gactctaaat taattaattc gctttgtaac tctatattgt caagcatcaa
      961 attatcgtat tcaaaactat tcctgagtct ttctactatt tctgctgtaa tagaattacc
     1021 agtaagacta gctgactgtt ctatttcttc ttttaaatca agaggaagtc taaaattaac
     1081 ctgggtatac tttttaatca tgaaattcta gagtctaaaa tgtcttgaat aactatagca
     1141 catttgctat atattattat agcaaatgtg ctataacatt tggagtagct atatatgtcc
     1201 aacattattg aatttaaaag aaatgaaagg agatttatta atcaaaaacc tatcagtcct
     1261 gtagaactag tgacaaatat actcactcca caagaggtag atataaagtc tttgattaag
     1321 gaaacaaaga aaattttaag agaaaaaggc ttaaaattta gtacttctca tcaagatttg
     1381 gaacacttaa aaggaaataa tttaatatta ttttccaaaa agaaatgcgg gtttatctta
     1441 accacaaagt tagcctgtag cacaaatagc tcatgggaaa tatattccat aagtatagat
     1501 aagacgacga aagcattaga tattgttaat aaaattatat ctaggctagg agataacgtt
     1561 catggcagca gcagagaaga agcaatcagc taatccagcg atggaagcag cgaaagaggc
     1621 agcaaatatt aaaattgatg gctttgagca aggtgtaaat acctacaatg cgattggttc
     1681 acaattattg cgagaaaagc gagatccaag tgtaattaat aaaactcgtg aaaatagacg
     1741 aaatattcga aaagcacgag aagctatcaa gaatttatag taaaaagctt cttaagatct
     1801 aaaatataaa aaattctcat taaacaagcc ctaagaaatt ctcagggctt tgttcatgat
     1861 aaaaattatc agaacatgtc ttctgcaaaa gtgactaaaa caaggctgtg aaactccaat
     1921 tccgtatata cacaagcatc tagtctaaga aatttttcct tatcagatgg aaaattataa
     1981 ggtacagacc aaggttttat taaacaagga cttaccttat caaaagttcc aataaattcc
     2041 tttttagtta aatcataaat tctgattaca cttaacttaa caatactcaa aacccaaagc
     2101 atgtggccct tataggtctg ataattatca caaaccagca caccatactt ttttaataag
     2161 tatttgtata taaatgagga aactccattt cgctgataat tggctaaaat cgatgcacct
     2221 tttatttgtt tacctgttat tttaatagag tcattaataa tttcgatatc tttaaatgcg
     2281 tatctcgcta tcaaaatttc tttctctact tcataagctt ctaaggcttt attagcagcg
     2341 tcatatcatc taaatattca tcagcaacag cacaaacccc atccaatgca tactcattat
     2401 taaattcaca ataaaaaagc tcaatacatc cagaatactc gctaccttct gaagcttgca
     2461 attcttttaa acaaaatatt ccaccttccc tttggttgta gaagtactta ctttcaagaa
     2521 tattttcata agaaaagtaa acaggtagtg gaattaaagg cttattataa ttttcttcga
     2581 gaagtatgtg tggaggagtc aatttaaacc ttatatttca tcaagctact aatgttggtt
     2641 taataatatc attaatttta gcttctaaca ttttctcata agaagttaat tctggatact
     2701 gtttggatgc tttaatagaa aatcttaagc tattatttct ttcttgaatt tcagctacaa
     2761 ctttcacatc atcccaatct tttttattga gaataatatc tttgaaaata agtatatatt
     2821 gcttattttt ttctttctca gaaaaacgtc ttagttcaaa tgtttgacga atattaggca
     2881 tagtaaaacg ctgaactaaa tcagtactat ttttgagggt ttgaacagtt ctagtgaaca
     2941 ttttgacacc tacataccta aagaaataaa agaaaataaa aatatatagt gacaggttat
     3001 aagacaaata tgtcactatc aactacgtca aatttggcgt actatatata tattaattta
     3061 tatatatata tgaatagggt tttaaaaaat tatgttaaat tttgaattaa aagaaaagtg
     3121 gggcgaaaac tctctaatat tagggttcac gcagatccca actactctaa tttatgcaca
     3181 aaaagagctt ggtctatcat ctatagaaat taacatctta cttaacttat tgacccattg
     3241 gtggaagaaa gaagaatttc cttatccatc tcaagctgga attgcatatc gaatgggagt
     3301 ttctactaga actgtacaac gaacattagc aggtcttgaa acaaaaggat tcataaccag
     3361 aaacaaaaca tcaagagata acagtaaata caaaggaagg agtatttatg atttatctcc
     3421 attagtaaaa attctggaag aaaaggcacc tgatctagat attgtgaaaa aaattaaaaa
     3481 aaataaaaga ctagctaaat agatttaaca taaaatctct tacgataagt caaaaaaggg
     3541 agcttaaagc tccctttttc tatctaacta tatgaatctt cggtgatgtt tgtataccta
     3601 attgcctgaa aatatgccat agagctatgt tgcgatactg gtacatagca atttgtgaaa
     3661 aatcaaacca ttgttcggat gtgaattggt ttatatcaga aaaaggtgga taacctctta
     3721 gagctagaaa tgcattaatt tcttctacca ttggatcgag ctcaaacatg ttttaaaacc
     3781 taataaagaa taaagtccaa ttgtaaatcc cctttaaaag gtcacttttt tattgttcca
     3841 aatcctcttt acagtttttt tgttctctac tttacaattg acttgggaaa ttatcccgat
     3901 gtttgtccgc aggaaatggg gataaaagtg ggttattgga gcattgagaa atcaggaaaa
     3961 attaaatttt tgagtatttt ttaatcgatt ttgttggcta aaagtgacaa agttgcttaa
     4021 aaagcatccg acttgtttcc aagtcggctt ttcacctatt ttttagtcat gactaattct
     4081 ataaataatt aaaaaataat aacttttcat aatttatttc gcataacgcc cattatgtta
     4141 aattaaaaga gttttaactt ttttaataaa aatattatac tctaagcccc aaaatttccc
     4201 ctaacatgaa tttgtaatga aaaaatttat acttcctata ttcagcattt ctattctagt
     4261 ttctctcagt gcatgttcat ctattaaaac taaatctgaa gataattttc atatttcttc
     4321 tcagcaacat gaaaaagcta ttaaaagcta ttttgatgaa gctcaaacac agggtgtaat
     4381 tattattaaa gagggtaaaa atcttagcac ctatggtaat gctcttgcac gagcaaataa
     4441 agaatatgtc cctgcatcaa catttaagat gctaaatgct ttaatcgggc tagaaaatca
     4501 taaagcaaca acaaatgaga ttttcaaatg ggatggtaaa aaaagaactt atcctatgtg
     4561 ggagaaagat atgactttag gtgaggcaat ggcattgtca gcagttccag tatatcaaga
     4621 gcttgcaaga cggactggcc tagagctaat gcagaaagaa gtaaagcggg ttaattttgg
     4681 aaatacaaat attggaacac aggtcgataa tttttggtta gttggccccc ttaaaattac
     4741 accagtacaa gaagttaatt ttgccgatga ccttgcacat aaccgattac cttttaaatt
     4801 agaaactcaa gaagaagtta aaaaaatgct tctaattaaa gaagtaaatg gtagtaagat
     4861 ttatgcaaaa agtggatggg gaatgggtgt tactccacag gtaggttggt tgactggttg
     4921 ggtggagcaa gctaatggaa aaaaaatccc cttttcgctc aacttagaaa tgaaagaagg
     4981 aatgtctggt tctattcgta atgaaattac ttataagtcg ctagaaaatc ttggaatcat
     5041 ttaataattt ctaaaattaa cataatacgc cttatgcgaa atttgcctga atagcctttc
     5101 tcagaaaggc tattactgat ttttcactaa agctttctta atttcgatga ctgacaataa
     5161 ctctccgtct tttttccagg tattcagtcc ctctaatacc tgatgaaaaa gcttatttct
     5221 ttcaaaatat ccatttttat aacttttaac ttcttgatat atcttagatt tcattacatc
     5281 atcatgaaat tttttcgtat tctgaatgcg attcttcatc tgttcaggcg tatcttgact
     5341 aatatcaatc cctaactcct tgaaagcagc taacacagca ccgcccaact caaacttacg
     5401 atgattttca gccgttcttt ttttgctggc ttccttcttc tggatctgac tcaaataaag
     5461 ctctaaatca ctcaactcaa gcttatgcgc tttgaaactc agataacgag tcgccatggt
     5521 tttcaagata ttaaaatcag caccactcaa cgcattgtcc cgataagcct caaacaactg
     5581 cacaaacttc cgttcatatg cggtctgcgg atacttcaca gacacctcat caaacaataa
     5641 gttcagcttc atatgctcac gataaggttt taaggcctct ttcaccaaga tctgttcagc
     5701 ttcagacaat gactcaaagt taaatttttc aattcgtagc attgcaatca catcattctg
     5761 caaatcagaa cgatccttct tctgtgaaag atagtctaca aatgcatgta actctgcgat
     5821 ttgtaaaagt tgtgcaggtc tgccacgttt ttttgattta tccaatgcca tcatctgccg
     5881 cctaattttc agaactgcta ccgattgagt tatggtttta ccataattca atcgggcagt
     5941 ctgaaaataa ttatttaaaa taaaaataaa atttcttgaa aagaatttat atttttattt
     6001 taaataattc gcacttgaca gtttctccta atcggagaaa ctagtacgca atggggcgat
     6061 tcccccttga ccccctttaa ccggattatt tcagccctgt aggcattaca taatccggag
     6121 atttttattg cgtttcactc cataaaaacc aacaacaccc caaggaagtg aacatggcca
     6181 gttaccattt cagcgtcaaa agtaaaaata aaggctacgc actgagccac tacctgtaca
     6241 tttcccgact catgcaatac gaaggcatcc gcaaaaagag tgcagaagtc ctcgaacatg
     6301 tcgaaccagg gcaatgcatg ccctcctggg tcaaagatcc ggttgaattc tggcaagcag
     6361 cggacacctt tgaacgggcc aatgccaagg cttacatgga atacgaaatt gccctaccga
     6421 atgaattcac cgtagaacaa cgtaaaacgc tcgtagagac atttgttgat aaaaccatag
     6481 caacacacca ctaccctcat tcctacgcca tacacaacgt aaaatcacgc ctcagtggtg
     6541 aagaacaacc ccattgccac ctcatgtttt cactcaaagc cgatgacggc atagaacgaa
     6601 gtccggaaca atacttcaaa cgctacaacg ctaaagaacc aagcaaaggc ggagcaaaga
     6661 aaattcaact acaggatggc catgccgact acaccactta cctcctacat atccgaaaag
     6721 cctgggaaaa tcacctgaat gatgcattgg cagaacatag cccaaccgtg acttaccaga
     6781 tcgatggcca gaagatccaa attaaaaacc aagtttcggc agacaactac gaaaaataca
     6841 acgagataca cggcaccctc tacctaccag aacccaaact ggggacaggc aaacaaaatg
     6901 aaaccgtgga atacctagcg gaaatccaga aaatcagaca acacaatgcc aaagaacgag
     6961 aactggaaca gcgccaacaa tactttgatc aacagcatga atactactac agctgctcaa
     7021 acgaaccctt cagttctccg gaagtgatgc actacctcaa tcacctcatt aaaaaagcca
     7081 gtaacaaaaa agaactgggc cacgatatcg acgaaattat tcaaactcag caaccaatcc
     7141 tgcatcacga aaaatgggat atcgatacca tcaattttaa tctcaataac gccaggttat
     7201 acccagaacc ggatatggag tttaaagtcc gatccgtaca accaaaacca gtgattgaac
     7261 caacatcacc gaagtcaaac ctgaaataca accctcacag cctgaaccga aacgaaatga
     7321 tttatgatgg tccgggtttt taaaaatgga tctagatcag aaagtacaaa gcgagtgtct
     7381 acgagcgaca tcaccaaaat tagcgcctcg actttctgaa aaaccgcttt ttaaggctgt
     7441 atagcctaaa aaagcataaa tcagatgcta aaactggctt ccagaccgag cgaagcgagc
     7501 aaaaagcttt atgagcgaag cgaattccga gttgcttttg ctcttgcctt tactttttct
     7561 caaattcacg aaggaattaa cagaaaaatt gccccctgaa tcgagcgaaa gcgagattca
     7621 atagagtttg agcgtagcga aaaccacggg caattttttc aaacgtcttt tttaattatt
     7681 tttatatctt ttaaatcttt tctttgagct gtatccctta tggagcaagc ctttcaaagt
     7741 ttaactatga cggattgact actaactatg acggattgac tactaactat gacggattga
     7801 ctacttaact atgacggatt gactactaac tatgacaagt atatagttgt catatataaa
     7861 agaaaatcaa tggttttatt ttggataaga atcaaattgt taaatcaaat caagttatag
     7921 aagcttccta tcaattaagt gctgtagaac agcgtatcgt cttggcagct atttcacgta
     7981 ttccgaagag ccagcctatt actgatgatg agttataccc tgtaagcgtt aatgaactac
     8041 agttattagg agtacatgaa aaaaccgcat acagagactt aaaagaagga attaatagac
     8101 tttatgaaag atctattaat cttagtattg atgataaatc tataaaaatg aggtgggtac
     8161 aagagatcca gtttttggat agtcaaagta tcattggtat tcgtttttca aaacctatct
     8221 tgcccttcat atctaattta agtagagaat ttactaagta tgctctatca gatattgctg
     8281 ggatcaatag tggatatggt attcgtattt atgaattatt ggtgcaatac agacaaatag
     8341 gtaagcgtga aatctctgtt gagaacttac gaagcatgtt ggagcttggc aaaaaatacc
     8401 cactatttgc agatttcaaa aaacgggtaa ttgatactgc tgtagaccaa ataaatgaat
     8461 gcagcccatt aagcgtttcc tatgagcaaa aaaaaactgg tcgtaaagtc actcatattc
     8521 agttttcatt taaagaaaag tccaaaagca ttaatcatct caatgagcca gagaagtttt
     8581 ataaattaac tgatgctcaa gtccatatgt ttggcaatca actttctcgt ctacatgaac
     8641 tatctcattt agcgactcaa ggagaaagtt acgatgatct agctgcaaca atcaaagaaa
     8701 tgctaagaga tccaatacaa caaaagcaat ttattcctca tcttaggaat ttaggtttta
     8761 aaggatgatg t
//
