LOCUS       NC_012813               8963 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Acinetobacter baumannii plasmid pABVA01, complete sequence.
ACCESSION   NC_012813
VERSION     NC_012813.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14229501
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1
  AUTHORS   D'Andrea,M.M., Giani,T., D'Arezzo,S., Capone,A., Petrosillo,N.,
            Visca,P., Luzzaro,F. and Rossolini,G.M.
  TITLE     Characterization of pABVA01, a plasmid encoding the OXA-24
            carbapenemase from Italian isolates of Acinetobacter baumannii
  JOURNAL   Antimicrob Agents Chemother 53 (8), 3528-3533 (2009)
   PUBMED   19487447
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   D'Andrea,M.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2008) D'Andrea M.M., Dipartimento Di Biologia
            Molecolare, Universita Degli Studi Di Siena, Viale Bracci, 2 -
            Siena, 53100, ITALY
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 11/28/2023 04:40:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 11
            CDSs (total)                 :: 11
            Genes (coding)               :: 11
            CDSs (with protein)          :: 11
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FM210331.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="VA-566/00"
                     /isolation_source="bronchoalveolar lavage fluid"
                     /db_xref="taxon:470"
                     /plasmid="pABVA01"
                     /geo_loc_name="Italy:Northern Italy, Varese"
                     /collection_date="2000-09-26"
     gene            663..1613
                     /gene="repM"
                     /locus_tag="HXF45_RS00005"
                     /old_locus_tag="pABAVE01_01"
     CDS             663..1613
                     /gene="repM"
                     /locus_tag="HXF45_RS00005"
                     /old_locus_tag="pABAVE01_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001208776.1"
                     /translation="MRELVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHAEGYINQFGVHRNTAYQALKDACNDLFARQFSYQKINERGNIENYRSRWVSEIGY
                     VDNEAVVKLIFAPAIVPLITRLEEHFTKYELQQVSNLSSAYAVRLYELLIAWRSTGSTP
                     IIEVSDFRQRIGVLDTEYKRMERFKTSVLELAIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDNPPIERDPNTLDLFTKMTDAQRHLFANKLSELPEMGRYSQGTESYPQFAI
                     RIAEMLQDPDRIKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            1606..2181
                     /locus_tag="HXF45_RS00010"
                     /old_locus_tag="pABAVE01_02"
     CDS             1606..2181
                     /locus_tag="HXF45_RS00010"
                     /old_locus_tag="pABAVE01_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_012754352.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTPQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATASPQQDATACDTDMQQHATPQQDNDNKKNHELNIAENVAMPQQENTAYHTQTLQH
                     ATLQNVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            2193..2336
                     /locus_tag="HXF45_RS00015"
     CDS             2193..2336
                     /locus_tag="HXF45_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2519..3346)
                     /locus_tag="HXF45_RS00020"
                     /old_locus_tag="pABAVE01_03"
     CDS             complement(2519..3346)
                     /locus_tag="HXF45_RS00020"
                     /old_locus_tag="pABAVE01_03"
                     /EC_number="3.5.2.6"
                     /function="hydrolysis of beta-lactams"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
                     /protein_id="WP_012754353.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MGVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
     gene            3655..3942
                     /locus_tag="HXF45_RS00025"
     CDS             3655..3942
                     /locus_tag="HXF45_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3929..4243
                     /locus_tag="HXF45_RS00030"
                     /old_locus_tag="pABAVE01_05"
     CDS             3929..4243
                     /locus_tag="HXF45_RS00030"
                     /old_locus_tag="pABAVE01_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            4371..6782
                     /gene="znuD2"
                     /locus_tag="HXF45_RS00035"
                     /old_locus_tag="pABAVE01_06"
     CDS             4371..6782
                     /gene="znuD2"
                     /locus_tag="HXF45_RS00035"
                     /old_locus_tag="pABAVE01_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_012754356.1"
                     /translation="MLNKSKLFLALITLGASKIVLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            7087..7548
                     /locus_tag="HXF45_RS00040"
                     /old_locus_tag="pABAVE01_07"
     CDS             7087..7548
                     /locus_tag="HXF45_RS00040"
                     /old_locus_tag="pABAVE01_07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(7871..8044)
                     /locus_tag="HXF45_RS00045"
     CDS             complement(7871..8044)
                     /locus_tag="HXF45_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282482.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQD"
                     /name="hypothetical protein"
     gene            complement(8044..8415)
                     /locus_tag="HXF45_RS00050"
                     /old_locus_tag="pABAVE01_08"
     CDS             complement(8044..8415)
                     /locus_tag="HXF45_RS00050"
                     /old_locus_tag="pABAVE01_08"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504219.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWTLMKNGASKDEV
                     RMMQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(8686..8940)
                     /locus_tag="HXF45_RS00055"
     CDS             complement(8686..8940)
                     /locus_tag="HXF45_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014301.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMKPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     ARG             complement(2523..3346)
                     /name="blaOXA-24"
                     /gene="blaOXA-24"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-24"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          664..1613
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.264"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aactttaatt atgaaaagct tcacagaaag cgttcaaatg cgatttaaga gcctttactt
       61 aaaaaacata gatctcatag tgaaaaacag aaaacagccc aaaaaacgca aatgagaacg
      121 aaataaagag agattttaac tttagataga tgcatagagc gagtgtctac gagcgaacta
      181 tcaaaatttg cgcctagacc ctctgaaaaa catttttttg tcctctttag cctaagaaag
      241 cgtaagtttc atacagaaat ttgctcctgg atcgagcgta gcgagtaaaa aagttttatg
      301 agcgaagcga attccgagtt gcttttgctt tttcttaaag tcacgcaagt attaaccaaa
      361 aaattgcccc aacgaactga gcgaaagcga agttcaatag agtttgagcg aagcgaaaac
      421 caagggcaat ttttcattcc ctgggctttt aattattttt aatcttttaa atgcttttag
      481 acatgctgaa agccttaatt tacaacactt tcaaaggcta tatgacacca tttaccttgc
      541 aatatgacac cgtttacctt gcaatatgac accgtttacc ttgcaatatg acaccgttta
      601 ccttgcttat agcactaaaa ttaattattg tgtcatagca taataaataa tactggtgct
      661 tcatgagaga attagttgta aaagacaatg ccttaattaa tgcaagctat aacttagatt
      721 tagtagaaca acgtttaatt ttattggcca ttgttgaggc aagagaaagc gggaaaggta
      781 ttaatgctaa tgatcccctt gaagtacatg cagaaggcta tatcaatcaa tttggcgtac
      841 atcgcaatac ggcttatcaa gcattaaaag atgcctgtaa tgatttattt gcaagacaat
      901 ttagctatca aaaaataaat gaacgaggga atattgagaa ctatagatcc cgttgggtta
      961 gtgaaattgg atatgtagat aatgaagcag tggttaaact tatctttgcc ccagccatag
     1021 ttcccttaat tacacgctta gaagagcatt tcactaaata cgaattgcag caagttagta
     1081 atctcagcag tgcttatgct gttcgcttat atgaattatt aattgcttgg agaagtactg
     1141 gctctactcc tattatagag gtaagtgatt tccgtcaaag aattggcgta ctcgatacag
     1201 agtacaagcg tatggaacgc tttaaaacta gtgtacttga gcttgctatt aaacaaatta
     1261 acgaacatac agatatcact gtgaagtatg agcaacacaa aagaggtcga tcaatttcag
     1321 gattctcttt tacttttaaa cagaaaaaga aggacaaccc accgatagaa agagatccga
     1381 atacgttaga tctctttaca aagatgactg atgcacaacg ccatctgttt gcaaataaac
     1441 tttctgaact tcctgaaatg ggtcgttatt cccagggaac tgaaagctat ccgcaatttg
     1501 ctattcgtat tgctgagatg ctgcaagacc ctgaccgaat aaaagaacta tacccatacc
     1561 taaaaaaagt gggatatatg ccatcaaata aaaaggacac cgtaaatggc taagttatca
     1621 ctaagtgaag tatctaaaaa attccatgtg gatagatcaa ccatttacag agctgtacgt
     1681 aatggacgtt tatcacgctc cagtgatgga caattcgatc tagcagaggt catccgatgc
     1741 tttggagaac ctgagcaaac accccaaaaa attgaatcat ctaagcaaga aggtgatgaa
     1801 tctacaaaaa aacttattgc ccatttagaa aatgaagtca aaaaatacca agaacgtgaa
     1861 gaacggttaa tgcaacaaat tgaccgtatg caaacactca ttgagctgaa aagtgttgca
     1921 cctgccacag catcaccaca acaagatgct acggcatgcg acactgacat gcaacagcat
     1981 gcgacaccac aacaagacaa tgataataaa aagaatcatg aattaaatat tgcagaaaat
     2041 gtggcaatgc cacagcaaga aaatacggca taccacaccc aaacgctaca gcatgccacg
     2101 ttgcaaaatg tggcagtgcc acaacacaaa aaacgtggct tatttggccg tgtgctgaat
     2161 gctgtttttg ataatgacta agggagggga tcatgccgaa actgaaagat attgccctgg
     2221 gaattattgt agctccgctg ctgatcccga tcatgctgat tgcatcgtac caggataaaa
     2281 aggcgctgaa gaaagagctg gatgaatgcc aaaaagaaaa agaccaggca tcctgattat
     2341 tcaattgagc taatcaaaag tgtggaacat tgatgaaata atagcgtgga acgtgaaaag
     2401 tcataaaaaa agcccgaaca gggatgctcg ggctataaac tagaaactac agcgttgaat
     2461 tataaggata attatacagc atttcgtata acgtgtatta tgttaatttt agaaattatt
     2521 aaatgattcc aagattttct agcgacttat aagtaatttc attacgaata gaaccagaca
     2581 ttccttcttt catttctaag ttgagcgaaa aggggatttt ttttccatta gcttgctcca
     2641 cccaaccagt caaccaacct acctgtggag taacacccat tccccatcca ctttttgcat
     2701 aaatcttact accatttact tctttaatta gaagcatttt tttaacttct tcttgagttt
     2761 ctaatttaaa aggtaatcgg ttatgtgcaa ggtcatcggc aaaattaact tcttgtactg
     2821 gtgtaatttt aagggggcca actaaccaaa aattatcgac ctgtgttcca atatttgtat
     2881 ttccaaaatt aacccgcttt acttctttct gcattagctc taggccagtc cgtcttgcaa
     2941 gctcttgata tactggaact gctgacaatg ccattgcctc acctaaagtc atatctttct
     3001 cccacatagg ataagttctt tttttaccat cccatttgaa aatctcattt gttgttgctt
     3061 tatgattttc tagcccgatt aaagcattta gcatcttaaa tgttgatgca gggacatatt
     3121 ctttatttgc tcgtgcaaga gcattaccat aggtgctaag atttttaccc tctttaataa
     3181 taattacacc ctgtgtttga gcttcatcaa aatagctttt aatagctttt tcatgttgct
     3241 gagaagaaat atgaaaatta tcttcagatt tagttttaat agatgaacat gcactgagag
     3301 aaactagaat agaaatgctg aatataggaa gtataaattt tttcattaca aattcatgtt
     3361 aggggaaatt ttggggctta gagtataata tttttattaa aaaagttaaa actcttttaa
     3421 tttaacataa tggcgattat acgaagtaca tttgtatatt aatttaaata tcaactactt
     3481 aataaaaata gaaaaatcca aaaatgccca aaaagccgac tttgaaacaa gtcggctttt
     3541 ttatgagcag ttttgatact tcttgccaat aaatttgact aaaaattacg cttttcagct
     3601 tgagtgatac aaatatgtga tacattattg tatatacaaa tttgaatgtt ttttatggaa
     3661 cagtattttg aatgggatga ggctaagaat cgaaagaatc agaaaaagca tgatatctct
     3721 ttcgaaactg caagccttgt ttttgaagat ccattacgga tctcaatcca agacagacat
     3781 accaatggtg aagaacgttg gcaaaccatt ggacgagtta aaggcgtact aatgttattg
     3841 gtggctcaca ccatttttga tgaagatgac tgtgaaatca tccgaatcat tagtgcaagg
     3901 caagtcacta aagcggagcg aaacaaatat gagcatggtt agatactcac acaaagagct
     3961 gaatgagaaa tttggtgaga agcaagatgc tgaaattcaa cgcttgcttg ctaaaggcac
     4021 ggttcctgat gatcagctag atctatcaga tattcctgaa attacagact ggagcaatgc
     4081 tgtacgccaa aatcaatttt accgcccagt aaagcaacaa acttcaattc ggttagatgc
     4141 tgatgtactt gcttggttca aagcacaagg caaaggttat caaacacgta tgaatgaaat
     4201 tttacgagat gctatgctaa aagaattaaa aaatcatcaa taagaatggg agcttaggct
     4261 cccatttttg acttcatttt taggggggat taccattcaa aaatttaaat agtagatttt
     4321 ataaattttt aagtgttata ttataatata acattaaatt tggttgtttt atgctaaata
     4381 aaagtaaact atttttagct ttaattactt taggggcctc gaaaattgta ttggcagctg
     4441 aagggcctgt tactacctta aatacaatag tattaactgc acaatctgat gagttagggt
     4501 ctgagctgtt aggcaaaagt ctaaatgtta gtaatcagtt tattgatact agtaaactta
     4561 aacagcgttc gacaactcta ggggatgcct tgggtacgga attaggaatt cattctaatc
     4621 aatatggagg tggagcatct actcctatta ttcgtgggca ggaaggaaaa cgtattaaag
     4681 ttttacagaa taatgctgac gtgttggata tgtctaatat gtccccagat cacgctgtca
     4741 cagttgaacc aagtttagct aagtctattg aaattatacg tggtgcaagt acccttttgt
     4801 atagttcaaa tagtgcggca ggggtagtca atgtcattga ctataaaatt ccaacccaaa
     4861 tgcctcaaga cggattagag ggtaatacta cattacgttt taatactggc agtaatgaga
     4921 agttgaccac agcaggtgta acagttggct taagcccaca tgtagcttta cgggcagaag
     4981 ggttatatcg taatgctggt aactataaaa ctcctcatta ccagtctagc tcttataatt
     5041 ctttagaaga tcttgaaaac caaaacagta tttataaaaa tttaaaatat ttaccagaaa
     5101 gctgggcaga gtcgagagta ggtacgcttg gattatcctg gatagacgat aatacttatt
     5161 taggagtttc ttatacacat cgtcatgatg aatatggttt accagcccat agccatttat
     5221 atgaaggttg tggtgcctct gcaatcggta tcgattcacg aattagtgga ttaaaaaatt
     5281 atcttttata ttatcctcaa ttgatggatg agcaagatat taattacata aatccacgtc
     5341 ctgattgtca tcagcataat catattcatg aaactaattt ctctcataat gctccttata
     5401 ttgatttaaa tactcgacgt tatgatgtgc gtggagaatt cactcaacca tttaccggta
     5461 ttgacaaaat tcgtacctca ttgagttata tagattattt tcacaatgaa ctcgagggag
     5521 ataagattac taactttttt aaaaatacag gaaaagtagg ccgcatagag ttatcacatc
     5581 agccattagg ggaattaacc ggtatcttgg ggctacaata tttggaacag gataatagtg
     5641 ctttatcacc agtccattct caagaaggcc ataccaccta tctagataat cagcagctat
     5701 taaatcgaaa tgtcactaaa aactttagtg tatttggcct agaaaaatat aactggaacg
     5761 actttacttt cgaactaggt gctcgtatag aaaagcagaa agttagtatg gattatgata
     5821 tagaaaaaat taaagattca atgaaacctt ggcctaataa atataacagt ccttatgtag
     5881 aaaaaaataa taaaatcagg gctcaaaatt taaaatctat tttagaagct gttcaaccta
     5941 acaaagaaac agctttttct tatgcaggta cagtccactg gagatttgct ccaaattata
     6001 tcttatcatt aacaggtaca catcaagaaa gattacctaa tgcacaagaa atgtatactc
     6061 atggtatgca tctcgctact aattcttttg aaataggtaa ccgctttcta agaaaagaga
     6121 aatccaataa tttagaaatc agtctggcct ataaggatga tttacttgat tatcaaatca
     6181 gtacttacta ttatgatttt gataactata tatatttaca gacactcaat gaagttttag
     6241 gaactacaaa agttagagat cagcatacac tacgtattaa tcattatagc cagtctgctg
     6301 ctaattttta tggattagag ggcaacatag ggtatcaatt taattctgtt tatcacggct
     6361 ctctttttgg agattatgtg aaaggccgtt taacaaattt acctgatgct gtaattgcat
     6421 acgatatctg gaatagagaa ccgactttag ctccccaaaa ggatcgttac actcctagat
     6481 tacctcctgc tcgtttgggt acacgactaa aagctgattt tgatgaatcg cttaaaggtg
     6541 aaatagaata ttatcgagtc tttaaacaag ataatatttc taaatttgag caggttacat
     6601 caggttataa tatgcttaat atgactttag catataaaaa taagctatct cacacagaat
     6661 atgatttatt ctttaaagcg aataatttac ttgatcagaa agtctatgcc catgaaacat
     6721 ttttgccata tattccccaa attggaagaa attttagttt aggcttaaat cttaatttct
     6781 agataagata ctgtaatctt ctaagaaaat ttcttagaag atttttctta taaaatgatt
     6841 agattaataa ttaaggtaag cgtccgctga atcccatacc tattttttaa ttcgatttag
     6901 gtttccagca gtgtggcagt aactcttcaa tctgggtcac tttatgtgtc ggcagccttt
     6961 tcagcactga atagccaccg attttgtaga caccttttag ctagataaaa agcaattttt
     7021 ttgatcttta aagaatgagg atataagctg tttaaaactt aattctaatt ggttaatagg
     7081 tgaaaaatga aattagcaga agctctctta cttcgaagtg atcaacaaaa aaaaattatt
     7141 tcactaaaac aacgtatcaa tgcaaatgta ttggtacaag atggtgatca accatctgaa
     7201 gatccaaatg aactactaaa acaagtattt tctttaattc aagaatttca aaaattaagt
     7261 tatgcaattc atgaaactaa tgctttaaca aaattaaatg atggccgttc tctacttgct
     7321 ttattgactt tgcgtgatga atttgtagaa cagcacaaaa ccctaactgc agcaataagt
     7381 aatacatctc gtgaatctga tcgttatagt acgcgggaaa taaagtggca taaagttatt
     7441 ccagtttcat cattacaaaa acaagctgat gatattagtc ttaaattacg tgatttaaac
     7501 gtgttaattc agtcgactaa ttggaaaatt gatttgattg aagcctaata gtcctttaga
     7561 tataatcaag ttgaacatac tgggcgagta caagatccct tacttccaca acataggatt
     7621 gtaaataaag tgtagtggct tgcaaagcaa gtggataagc ttaaaatttt aagtacaaat
     7681 caatccatca cttggcataa ctcaaatctt tgtgcaaaca aagttacacg ttacttataa
     7741 ctaccatgta ctgacagtat gttcacctca attactaaaa ataaagaaat gagcaagcat
     7801 cagctcaact acatttctat ttttaattca gttcggcatt taacataaaa tctcttatgc
     7861 gaagcccagg ttaatcctgg gctttttgtt caatctcatc attggccgag aacatacttg
     7921 gattcgtaag cctgatattt tcctgacaac ttccacacag catcaatcct gcaaaaagtt
     7981 gtacattttg tctattttca caaagcgtac atggctttga aagctgaata atattatctg
     8041 acactacttt accctcttat agttatcatc tgcatatgaa taacctttag cttgcatcat
     8101 tcgaacctca tctttagaag ccccattttt cattaatgtc cataacatca tgaaatagag
     8161 tccagaaaaa tcttcatagt atttttcaga aaaattatta tcttcctcaa aatcatctat
     8221 tcgcttttct aatcgattca ccagatcttc aatacgatct gcctgctgct taacagcaat
     8281 agattgaaaa tcattagtca aaattttact gaacaattta ctggttctga ttccttctga
     8341 attagccaat tgatcgtaga attctgctac ttgtggatct agtctgactg cataagtttt
     8401 gactttttcg cccataaatt acatctctta taaaaattag agtttgtaca agattaaatc
     8461 aagataattc aataatttaa attaaaataa taagtaaatt acgtacaaat ctatttgtac
     8521 aaaattaatc tattctaaaa caaaaactta tttaaatata atgctttttt tatgtacaac
     8581 tatgagtaca tgacttttat taaatttatc aatactttat ctaaaaaaag tatggttttc
     8641 atgtacaagc atgtgcacag cataaaaaac atataattca actacttatt ctaaaaaaag
     8701 accatttttc tgtacacgtt tctgagaccc taaaaaatcg ctgaaatcct tatcctgtaa
     8761 ggctttgagg ggtttcatcg ttagatgcgt ggtgtgtgac aaaaaaatcc cggcatgtgc
     8821 cggatcttgg attggaaaat tggctaaagt gacgtagggc cggtgcttgg ttttgcatgg
     8881 aaaaaagtat ttatttcctt gtttagaaaa acgtaaaaaa tcaatttttt gtccattcat
     8941 ccaggttaaa aatttcaacc caa
//
