LOCUS       NC_011799               5217 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid p5217, complete sequence.
ACCESSION   NC_011799
VERSION     NC_011799.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225757
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Handford,C.L., Stang,C.T., Raivio,T.L. and Dennis,J.J.
  TITLE     The contribution of small cryptic plasmids to the antibiotic
            resistance of enteropathogenic Escherichia coli E2348/69
  JOURNAL   Can J Microbiol 55 (11), 1229-1239 (2009)
   PUBMED   19940931
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Dennis,J.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2008) Biological Sciences, University of Alberta,
            Biological Sciences Building, Edmonton, AB T6G 2E9, Canada
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009952245.2-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 02:56:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9
            CDSs (total)                      :: 9
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            EU580135.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E2348/69"
                     /db_xref="taxon:562"
                     /plasmid="p5217"
                     /note="clinical isolate"
     gene            90..350
                     /locus_tag="HS503_RS00005"
     CDS             90..350
                     /locus_tag="HS503_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176703024.1"
                     /translation="MGLFMDQREKAKLLAYVLKTEWNAKQKHIALVLQVSEGTISLWVK
                     EMSLKHQIYKLSQELQEVRALAQGLVSSGQIEQNPGFLPSF"
                     /name="hypothetical protein"
     gene            366..875
                     /locus_tag="HS503_RS00010"
                     /old_locus_tag="p5217_01"
     CDS             366..875
                     /locus_tag="HS503_RS00010"
                     /old_locus_tag="p5217_01"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_012615286.1"
                     /translation="MAITFHPKKKQILICDFKGSVSPEINQCRPVLILREEIVGSQKLV
                     VVVPISNTEPNPLCSFHHELDDASLSGHMMGKKHWAKCDLVSTVSVDRLDRIRSRDPRT
                     GKNYFHVGVMTDDDFDAVRDKVMYGIGFDDFLDVKNEGVPDGAAASVVIAAEVNVAEVE
                     KQKKAI"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(1233..1379)
                     /locus_tag="HS503_RS00045"
     CDS             complement(1233..1379)
                     /locus_tag="HS503_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_230905658.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDKTPLAAAIGKRVYKRLLM
                     QSS"
                     /name="replication initiation protein"
     gene            complement(2051..2242)
                     /locus_tag="HS503_RS00015"
                     /old_locus_tag="p5217_02"
     CDS             complement(2051..2242)
                     /locus_tag="HS503_RS00015"
                     /old_locus_tag="p5217_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2306..2629
                     /locus_tag="HS503_RS00020"
                     /old_locus_tag="p5217_03"
     CDS             2306..2629
                     /locus_tag="HS503_RS00020"
                     /old_locus_tag="p5217_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955990.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     ALPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2838..>3269
                     /locus_tag="HS503_RS00050"
                     /pseudo=""
     CDS             2838..>3269
                     /locus_tag="HS503_RS00050"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3307..3825
                     /locus_tag="HS503_RS00030"
                     /old_locus_tag="p5217_05"
     CDS             3307..3825
                     /locus_tag="HS503_RS00030"
                     /old_locus_tag="p5217_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012615288.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGRIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            3832..4065
                     /locus_tag="HS503_RS00035"
     CDS             3832..4065
                     /locus_tag="HS503_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137441.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4668..5099)
                     /locus_tag="HS503_RS00040"
                     /old_locus_tag="p5217_06"
     CDS             complement(4668..5099)
                     /locus_tag="HS503_RS00040"
                     /old_locus_tag="p5217_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_012615289.1"
                     /translation="MKIPTHLKHKPVIQVENYDRIDGPYAEDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVSRSS
                     DNQELAHHTELMKKELKENQEYLDESLKRLSAELKRLGY"
                     /name="DUF6530 family protein"
     Typing          1877..1959
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="3.86e-36"
     Typing          complement(1308..1866)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.939"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2277..2623
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.828"
                     /coverage="98.0"
                     /evalue="1.61e-65"
     Typing          2620..4169
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.979"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tatttatagt gcatctggtt tttaatttac cgctgcgttg acagctttgt ctcgtttctt
       61 taaaacattt aaaatatttt aaatgtttga tggggctgtt tatggatcag cgagaaaagg
      121 ccaaattgtt ggcttatgta ttaaaaaccg agtggaacgc aaagcaaaaa cacatcgctt
      181 tagtactaca ggtatcagaa ggaactatct ctttgtgggt gaaagagatg tctttaaaac
      241 atcagattta taaacttagt caagaattac aagaggttag ggctttagct caggggctgg
      301 taagttctgg gcagattgag caaaaccctg gttttttacc tagtttctaa ataaatggat
      361 gctacatggc tataaccttt caccctaaaa aaaagcagat cttgatatgt gattttaaag
      421 gttcggtatc tccagaaatt aaccaatgcc gaccagtgtt gattttaaga gaagagattg
      481 ttggcagtca gaagctcgtt gttgttgttc ctattagcaa tactgaacct aatccccttt
      541 gcagctttca tcatgagtta gatgatgctt cacttagcgg ccacatgatg gggaaaaagc
      601 attgggctaa atgtgatctg gtatcaacag tgtctgttga ccgtttagat cgaattagaa
      661 gccgggatcc gagaacgggt aaaaattact ttcatgtagg agtcatgact gatgatgact
      721 ttgacgcagt aagagataaa gtcatgtacg gtatcggatt tgatgacttt ttggatgtaa
      781 aaaatgaagg tgttcctgat ggggcagcag cttcagtggt aattgctgcc gaagtgaacg
      841 ttgctgaagt agaaaaacaa aaaaaagcca tctgattttc atcaaatggc ttttttttga
      901 cccaagccgt tttcacggag tcatagtcta aaagttggtc aatcttttac aactagccgc
      961 ataaagcgga cttacaagcc cctaagggaa cactagtaat tatagtgtcg atattttaat
     1021 tatcaatagt tataagatcg agcatctgct tcttcttgag atcattaggg gttggaggta
     1081 atctcctgtc ctgtaaacga aaaaaccacc tggggaggtg gttttttcga ggggtcagta
     1141 agttggggaa cttctgaacc gtggtaacag ggtgtacaag accgctgcca ccaaaatcgt
     1201 ccttccagtt tagctgtagt ttggctcaga cttcaagaac tctgcatcag taatctcttg
     1261 tacaccctct taccaatggc tgctgccagt ggcgttttgt cgtgtctttc cgggttggac
     1321 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1381 cagcccagct tggagcgaac gacctacagc gagccgagat acctacagcg tgagctatga
     1441 gaaagcgcca cgcttcccga agggagaaag gcggagaggt atccggtaag cggcagggtc
     1501 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1561 gtcgggtttc gccacctctg acttgagcgt ctgtttttgt gatgctcgtc aggggggcgg
     1621 agcctatgga aaaacgccat cggcgtggcc tttttccggc tcttggtctt ttgctcacat
     1681 gttctttccg gccttatccc ctgattctgt ggataaccgc attaccgtct ttgagtgagc
     1741 tgataccgct cgccgcagcc gaacgaccga gcgtagcgag tcagtgagcg aggaagcgga
     1801 agagcgtcgg aagttgcatt ttctccttac acatctgtgc ggcatttcac acccggcatg
     1861 gcgcactttt catacatccg cactgatgcc gcatggttaa gccagtatac actccgctat
     1921 cgctacgtga ctgggtcatg gctgcgcccc gacacccgca aggtcaacgg cctgacgcgc
     1981 cctgacgggc ttgtctgctc ccggcatccg ctcacagaca agctgtgacc gtctccggga
     2041 gctgtatgtg tcagaggttt tcaccgtcat ccccgaagcg tgcgaggcag ctgcggtaaa
     2101 gctcgtcggc gtggtcgtga agtgattcac aaatatcggc ctgttcatct gcgtccagtt
     2161 cgttgagctt ctccagcagc gttaatgtct ggcttcttat aaagcgggcc atgttaaggg
     2221 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggt
     2281 aatgataccg atgaaacgcg agaggatgct cacaatacgg gttactgatg atgaacatgc
     2341 ccggttactt gaacgctgtg agggtaaaca actggcggta tggatgcggc gggtctgcct
     2401 gggggagccg gttgcccgtt ccgggaaact gccgacactg gcactgccgt tactgcgtca
     2461 gctggccgcc atcgggaata acctgaatca gacagcgagg aaggtgaaca gcgggcagtg
     2521 gtcttccggt gatcgggttc aggtgttggc tgcgctgatg gccatcgggg aggagctgcg
     2581 tcggctgcgt ctggccgtca gggaacaggg cgcgcgggat gattgttaaa tttcatgcca
     2641 ggggaaaagg cggtggcagt ggtccggttg attacctgct gggaagggag cgaaaccggg
     2701 aaggggcaac ggtgcttcag ggtaatccgg aagaagtccg ggaactcatc gatgccacgc
     2761 catttgcgaa gaaatacacg tcaggtgttc tgtcgttcgc ggagaaggag ctgccgccgg
     2821 gaggacgtga aaaggtgatg gcgagctttg agcgtgtact gatgcccggt ctcgaaaaga
     2881 atcagtacag catcctgtgg gtggagcacc aggacaaggg acggcttgag ctgaattttg
     2941 tcattccgaa catggagcta cagaccggaa aacgcctcca gccgtactac gaccgggcag
     3001 acaggccgag aattgatgcc tggcagacgc tggtgaatca ccattacggg ctgcatgacc
     3061 ccaacgcccc ggagaaccgc cggatactga ctctccctga taacctgccg gaaacgaaac
     3121 aggcgcttgc agagagcgtc acacgcggga tagacgctct ttatcatgtc ggggagataa
     3181 aaggccgtca ggatgtgatt caggcgctca cagaggcagg gcttgaagtg gtcagggtga
     3241 cgcgaaccag tatcagcatt gcagacccga acggtgggaa gaatatcagg ctgaaaggag
     3301 cattctatga gcaatctttt acagacgggc gcggagttcg agaaaaagct gaaagagaga
     3361 gccgaatcta cagagacaat gctgaacgac gagttcagca agctcgaaaa atctgtaagc
     3421 aaggctgtga catcaaacga gacgaaaatc aaagacgcta tagccctgtt cacagcttcg
     3481 acagaggaat cactgaaaaa acaccgggaa ggggtgaaag aggcgatgat gcagcacagg
     3541 aaggacgtgt taaagctggc agggaatacg ggcatgatgt tactggggat ggtcattttc
     3601 ctgtttaccg tgagtggcgg gacgctctgg tatcttggcg ggcggataca ggcgaacctg
     3661 gaagaaatca ggatacagga agaaacattg cagaaactga acgcgaagac atggggcgtg
     3721 gagtttgtgc aggacgggag aaggaaattt ctggtcatcc cgcaggggaa atcggcaacg
     3781 gtgattccct atcaggggaa ggactgggta caactgacgg agtgacacag agtgacggag
     3841 ctggaaagac atttgctgaa cgcattagag caactgcaac aggactatat gcagcggctg
     3901 agcgaatggg agagcgcctt cgtggaattg cagaagatgt tttcgcttac gcaacgggac
     3961 aacgcgatgc tgaacgagca ggtcatgcag ttgagtcagc aggtgcagca cttgagcgag
     4021 cggacagaac gcttgagcca gttatacagc gagaacagga gataagggac gaacggctga
     4081 tacaggagcg cgaacatgcg ttatccctgg agcgtgagcg ccagccggaa atacaggaac
     4141 gcacactgga tggcccttcg ctgggatggt aatcagagac aggctaaaat gttgtcagcg
     4201 cgtttccggt gcattttttg ggataatggt tggttcgggg gttccgggaa cgcgcttctg
     4261 tcagaccttg ctgaaaacac aggaacgcgt taaaacgcat acgggcgcga ttctggtgta
     4321 tcttttgttg ctgtgttttg cctctattgc cagatcgcgc tcattcccct gaacttctgt
     4381 ttttccgctc cggttccgtc ttctgccttt ccgaaagtat gaaaaaaatc cgtttcagca
     4441 gtctctggct ggcaattcct gcgtgattca catggctgca tagctatgca tgtggttaaa
     4501 atttatcggg tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt taccagtctg
     4561 ctgccgtgat cgcaccgaac gcgtttcagt ggtgcgtaca atagagatgt tatggtaaat
     4621 attacaggtg tgatggtatg aaaaagccgc ccctgtgggc ggctttttta gtaaccaaga
     4681 cgtttcaact cagccgacag acgttttaag ctctcatcga ggtattcctg gttttctttt
     4741 agttcttttt tcatgagctc agtgtgatga gcgagttctt ggttgtctga agaacgactg
     4801 accgggaaat catcactaga tgataaccgc ccatttttgc tgtaatccat cgttatgcag
     4861 atcagagtag caaggtctat tacgcggtga agtggaagct cctcagactg ccgagaccac
     4921 ttttcgccgg tatttcgcca aacctttgcc gaaagctctg tccatcctgg agtgttccat
     4981 tgagcaatac caacggatag tcccatggca tctgtatctt ctgcatatgg accatctatg
     5041 cggtcatagt tttcaacttg aattacaggc ttatgtttta aatgagttgg tattttcatt
     5101 gatagaaccg attgttgttt ttttgttatt aacaaaaaaa caaaatttat tgattagtaa
     5161 atttactaaa tgtaagtaaa tattattttg attatatata tttgattttt aatgcat
//
