LOCUS       NC_011795               6148 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid p6148, complete sequence.
ACCESSION   NC_011795
VERSION     NC_011795.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225756
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Handford,C.L., Stang,C.T., Raivio,T.L. and Dennis,J.J.
  TITLE     The contribution of small cryptic plasmids to the antibiotic
            resistance of enteropathogenic Escherichia coli E2348/69
  JOURNAL   Can J Microbiol 55 (11), 1229-1239 (2009)
   PUBMED   19940931
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Dennis,J.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2008) Biological Sciences, University of Alberta,
            Biological Sciences Building, Edmonton, AB T6G 2E9, Canada
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009952255.2-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 02:58:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            Pseudo Genes (multiple problems)  :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            EU580136.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="E2348/69"
                     /db_xref="taxon:562"
                     /plasmid="p6148"
                     /note="clinical isolate"
     gene            97..912
                     /gene="sul2"
                     /locus_tag="HS576_RS00005"
                     /old_locus_tag="p6418_01"
     ARG             98..909
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            973..1776
                     /gene="aph(3'')-Ib"
                     /locus_tag="HS576_RS00010"
                     /old_locus_tag="p6418_02"
     ARG             974..1773
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1776..2612
                     /locus_tag="HS576_RS00015"
                     /old_locus_tag="p6418_03"
     CDS             1776..2612
                     /locus_tag="HS576_RS00015"
                     /old_locus_tag="p6418_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<2719..3124)
                     /locus_tag="HS576_RS00020"
                     /old_locus_tag="p6418_04"
                     /pseudo=""
     CDS             complement(<2719..3124)
                     /locus_tag="HS576_RS00020"
                     /old_locus_tag="p6418_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /name="transposase zinc-binding domain-containing protein"
     gene            complement(3168..3590)
                     /locus_tag="HS576_RS00025"
                     /old_locus_tag="p6418_05"
     CDS             complement(3168..3590)
                     /locus_tag="HS576_RS00025"
                     /old_locus_tag="p6418_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3775..4215
                     /locus_tag="HS576_RS00030"
                     /old_locus_tag="p6418_06"
     CDS             3775..4215
                     /locus_tag="HS576_RS00030"
                     /old_locus_tag="p6418_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(4907..5056)
                     /locus_tag="HS576_RS00035"
     CDS             complement(4907..5056)
                     /locus_tag="HS576_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5627..5785
                     /locus_tag="HS576_RS00040"
     CDS             5627..5785
                     /locus_tag="HS576_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             1777..2609
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(5092..5265)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          5189..5794
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggatcaccgc aaacaggtta ctcgcctggg gattcccttt cgacccgagc atccgtatga
       61 gactcatgct cgattattat tattatagaa gcccccatga ataaatcgct catcattttc
      121 ggcatcgtca acataacctc ggacagtttc tccgatggag gccggtatct ggcgccagac
      181 gcagccattg cgcaggcgcg taagctgatg gccgaggggg cagatgtgat cgacctcggt
      241 ccggcatcca gcaatcccga cgccgcgcct gtttcgtccg acacagaaat cgcgcgtatc
      301 gcgccggtgc tggacgcgct caaggcagat ggcattcccg tctcgctcga cagttatcaa
      361 cccgcgacgc aagcctatgc cttgtcgcgt ggtgtggcct atctcaatga tattcgcggt
      421 tttccagacg ctgcgttcta tccgcaattg gcgaaatcat ctgccaaact cgtcgttatg
      481 cattcggtgc aagacgggca ggcagatcgg cgcgaggcac ccgctggcga catcatggat
      541 cacattgcgg cgttctttga cgcgcgcatc gcggcgctga cgggtgccgg tatcaaacgc
      601 aaccgccttg tccttgatcc cggcatgggg ttttttctgg gggctgctcc cgaaacctcg
      661 ctctcggtgc tggcgcggtt cgatgaattg cggctgcgct tcgatttgcc ggtgcttctg
      721 tctgtttcgc gcaaatcctt tctgcgcgcg ctcacaggcc gtggtccggg ggatgtcggg
      781 gccgcgacac tcgctgcaga gcttgccgcc gccgcaggtg gagctgactt catccgcaca
      841 cacgagccgc gccccttgcg cgacgggctg gcggtattgg cggcgctgaa agaaaccgca
      901 agaattcgtt aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg
      961 aaggaacctc cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg
     1021 cctgtcagag gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc
     1081 gcgaaaatcg cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt
     1141 tggctcaaag gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag
     1201 ggtgcatgct tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg
     1261 gatttgctca aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg
     1321 gttgatcaat gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg
     1381 gtgtcccgca atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgcag
     1441 ctcgatcttt tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc
     1501 gatatggttg tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact
     1561 cttcaatgca cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat
     1621 ttggcactca tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc
     1681 gccttcgctg tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc
     1741 ttctatctgc gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg
     1801 ctcattggca cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga
     1861 aagtttcatt gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca
     1921 ttgctgatga actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc
     1981 ggttgctcgg tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct
     2041 ctcacatcgt tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg
     2101 cgaagctgta tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc
     2161 gctttgcagc tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact
     2221 acgtccacgc ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc
     2281 tacatggcga tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag
     2341 atcccgtcgg tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg
     2401 ctgacagaga cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct
     2461 ctcgtgcgct ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc
     2521 tttccgcagc ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg
     2581 cgatcaagca ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg
     2641 gaacacatca atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc
     2701 cttcccgcaa acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg
     2761 acgcatgggt tgttcaggca gtacttcatc aaccagcaag gcggtcactt tcggccatcc
     2821 gccgcgcccc acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct
     2881 cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga
     2941 gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa
     3001 gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc
     3061 ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag
     3121 gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt
     3181 cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact
     3241 cccactctta cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt
     3301 ataatgttat taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca
     3361 gttatcgggt gtcggccagt gatgagacca aaggatgcag tataagatga accactacta
     3421 ttatcatggc caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta
     3481 tgaccatcat tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta
     3541 tttaactctg tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt
     3601 tataacaatg tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca
     3661 acaggttttt tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac
     3721 agtaatatgt tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca
     3781 tggcacacag ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt
     3841 gacatggcta aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag
     3901 accagtgaac tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac
     3961 aaggagggac actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt
     4021 cagcagaaac tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg
     4081 cgggaagcag aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc
     4141 cgtctggctg acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc
     4201 tccggttcag agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc
     4261 gcctgagagg ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc
     4321 cgaaaacccg ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa
     4381 gtgcatagcg cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc
     4441 ccatccgaaa acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg
     4501 cagccggaaa acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag
     4561 gatgaaagtg acgtatgagc tgaaggagcg atgtaattct gtagtcagga agaataaccc
     4621 gttcatgcag gcatgaacga tacagtgagg aacgcttttt ttgtcacgat ctccccttaa
     4681 cctgcgtaaa tcacccgcca tggttgatta tttttcaacc tcaaaatggt catacaccta
     4741 acgcagccgc gattttcatc gcaactgcat gaattaaaag aaaaaaaagg aatatttcgt
     4801 tttcacgagt aatacccggg tattaaaaat ccactgtaat tacatgaaaa taaagaaata
     4861 aaaggtaata ccggagtatt accacgtacg cgggcaagcg aaaaagctat aacgcttccc
     4921 ggccgacagg cgacccaaaa tccacagggg catgcaggtt ctcttcagta acctgcgcca
     4981 ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc agtcgaatcc agttgttcat
     5041 catattcaga tggcatgggg tgatatccag tagctgagag gttgttagcg ggtttcgggg
     5101 cgcagccctg aaccagtcat gtagcgctag cggagtgtat actggcttac tatgttgcca
     5161 cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga aaaaaggcag caccggcgcg
     5221 tcagcagaat atgcgataca ggagatattc cgcttcctcg ctcactgact cgctacgctc
     5281 ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg ggacggaaat ttcctggaag
     5341 ataccaggaa gacacttagc aaagaagaga taaggccacg gcgaagccgt ttttccatag
     5401 gctccgcccc cctgacaagc atcgcaaaat ctgacgctca aatcagtggt ggcgaaaccc
     5461 gacaggacta taaagatccc aggcgtttcc ccctggtagc tccctcgtgc gctctcctgt
     5521 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc tcattccacg
     5581 cctgacactc agttccgtga aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     5641 cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa cttgagtcca acccggaaag
     5701 acacgacaaa acgccactgg cagcagccat tagtaacagg agtgcacgag aagatagatt
     5761 aagaggtctt gaagtagagg cctgactgcg gctacactga aagaacagtt ttggtaatgg
     5821 cggtctcgtg aaccctgtta ccgggttaag cagttcccca actgacttaa ccttcgaaca
     5881 aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga gattacgatt cattgaaaag
     5941 gatctcaaga agatcctttg tagattacta tcaattaatc ttccgaacat gacaccagtc
     6001 actgttgatt acggttagac cgctccgtaa caggtttccc tcgtattggt gacttgcagc
     6061 accaaagaag caaaaaagat aaatatatct acttattgag agatgatata tttatttcca
     6121 aaatgctaca gatacatcat catgaaac
//
