LOCUS       NC_011640               4211 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae plasmid pKpn114, complete sequence.
ACCESSION   NC_011640
VERSION     NC_011640.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225285
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Zhu,W., Zhuang,X., Wang,J. and Lu,Y.
  TITLE     Complete Nucleotide Sequences of the pCTX-M360 and pKpn114 Plasmids
            in the Multidrug Resistant Klebsiella pneumoniae in China: Evidence
            for pCTX-M360 as an intermediate plasmid between pCTX-M3 and pEL60
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Zhu,W., Zhuang,X., Wang,J. and Lu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2008) Department of Biochemistry, Sun Yat-sen
            University, 135 Xingangxi Rd, Guangzhou, Guangdong 510275, China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/09/2024 01:56:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5
            CDSs (total)                      :: 5
            Genes (coding)                    :: 4
            CDSs (with protein)               :: 4
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            EU932690.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Name: pKpn114.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="0773"
                     /db_xref="taxon:573"
                     /plasmid="pKpn114"
     gene            complement(560..1192)
                     /locus_tag="HS552_RS00005"
                     /old_locus_tag="pKpn114_02"
     CDS             complement(560..1192)
                     /locus_tag="HS552_RS00005"
                     /old_locus_tag="pKpn114_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(1192..2307)
                     /locus_tag="HS552_RS00010"
                     /old_locus_tag="pKpn114_03"
     CDS             complement(1192..2307)
                     /locus_tag="HS552_RS00010"
                     /old_locus_tag="pKpn114_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2652..2765)
                     /locus_tag="HS552_RS00025"
                     /pseudo=""
     CDS             complement(2652..2765)
                     /locus_tag="HS552_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3181..3372
                     /locus_tag="HS552_RS00015"
                     /old_locus_tag="pKpn114_05"
     CDS             3181..3372
                     /locus_tag="HS552_RS00015"
                     /old_locus_tag="pKpn114_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3372..3773
                     /locus_tag="HS552_RS00020"
                     /old_locus_tag="pKpn114_06"
     CDS             3372..3773
                     /locus_tag="HS552_RS00020"
                     /old_locus_tag="pKpn114_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2653..3060)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 agctcggtac ccggggatcc tctagagtcg acgaacccga ttcctgaagg tgctgaaaac
       61 cggttttccg gctgcgccgg caacgcttca cggccacgca ataaattgcg tccccgttca
      121 ccgttttcgg aggagaagaa cgccggaaac aggacttccc ctggctgaat ggtgtgaatt
      181 tccgcgctat gcacttgcgc gcatactcat gcatgccgta aaaacagagc ctgcgcgttt
      241 ctggcgggtt ttcgggtggt ttgttgcctg ttttaccggt ttcccgtcag aaacgtcctg
      301 agtccgtttt agcggtgcgt acaattatga ttttatgtta aattgtttta agtgaataga
      361 gagaatataa cttaagggag catcttgtgc tgggtctttt acattagatc tgcacaatca
      421 ttgttttggc atctggtgat gtctttagga cgtttgagat tgctgataac taaagtgtat
      481 gatgctttct atacgttatc cggcgtttaa taacaccctg gtgataagcc agcccgtgaa
      541 gggctggctt gatttggatt tatatttttg aaataaaatc ttttatttca tcaaagcaat
      601 tgtctgctgt gtttattatt ccatttttca tggatattcc tattcttgag tcggattctc
      661 tattgcagct aagatagact tgtaccttag cttttttttc gtttgttgga taagtgtact
      721 ctttgtcact cgctgagatg tcatctttat tatttttaat tttttcgaaa aattctatta
      781 tgtattcata taacgctcga gtgcttgcgt gttcaattat cagatcttca atggcacctt
      841 gcttgtcttt tcctggcatt ataaagaaag aaatttttgt attgccattc atagctatct
      901 cgttatgcga gctgggggag gagatttgta gttctcgcaa tgtggcaatt attgatcttt
      961 ctgtgtcaga aaatgatgtg tcagcatcta atataataaa aatactttct ttttcatcga
     1021 aagaatcaat gtttttgaat gctctaagtg cttgttttaa ttttgatttt ccatttattt
     1081 tttcgacaaa aacatcggta atgccttgta tttcaagtag gttgcataga aaatgaaaat
     1141 catcatcacc ctcaacgaaa atgatctttt tgctattatt tatgtctttc attatcttac
     1201 ctcccagtcg ttctcaactg agtaggagaa atcatccata ttgaattgta cggctgttgg
     1261 ttttagggag ttcttttctc ttcctagtct aatataagcg atatcttcat ttttgctatc
     1321 tatttcgttt attgctcgta tcacatcttg actatgagtt gttgcaaata tttgattgtt
     1381 gttttgtttt gcagatgaaa ttaatgattt tattatgtct ttaattactg agtaatgtat
     1441 tccgttctcg atttcatcaa taagaataat tgagttttcc tgtaccagca ctgagctgat
     1501 aaaagataat gctcgactga taccctcgcc aagcattgat atttcattta gctcactaaa
     1561 tccaatgtct aaatatattt ccttattgtt acctatcgca gatattgcta tatctacaat
     1621 tctgtcatct atttttttta agttttctag tatgtctttt ttcctttttt gtgttagtag
     1681 gctagataca tttgtaattg ttgcctcttt gtttatcttt ctggatgttg ttattattgt
     1741 tgctgtctta aatttataat taatcccatc cctttcgttt tttatttcgg attttatttc
     1801 tacgccatct tctttcaatt gcatagagaa aagcttattg gaaaccctgt ttttcgcatt
     1861 gggtgtctcg gtcacattta tttttaaaga gtttactgtg gcctgagaac cgatagtttg
     1921 acgaggtatg ttttggttag atagtacggt gtcaacgttt agagacaaac ttgattctaa
     1981 tctctgattt gtttctgttt catatgtttg tgttacttca gagcgttcat ctcgaatgac
     2041 tatggacatc ttttgtgatg tgtccatatc atgaaagtaa ctaacccaaa atggcttgtt
     2101 tttgtttatt tctgccattt cttttctgaa tgcttgaatg tttaacaaaa cagccgggtt
     2161 ttttacatca taaaaacaaa aaatagcatc cagtatagat gttttcccgt agttgttttt
     2221 cccagctatg atatttaaag tctttaattg ctttagatat agctcattga agcatttgaa
     2281 gttctttaaa cttatttcct ttatcatgat agcctctgtt cgtgtttttg ttatatgcca
     2341 tacgatttgt tagtacctta ataaaatgta cagctttcat tgtgattagc tagactttat
     2401 gtcaaagatt aactttgatt gcttgtgttt taatcgattt tttttacttt cttcttgaga
     2461 ttatctggca gcagccgtaa gcttccggct gtgaacgaaa aaaaccgcct tggcgggcgg
     2521 ttttttcgaa aggttcagta gagttggcgc tctatgaacc gaggtaactg gcttaaaggg
     2581 agcgcagtca ccaaaactgt tcgtttagtt tagctttaac tggcgtataa cttcaagact
     2641 aactccctaa atcagttacc agtggctgct gccagtggcg ctttgtcgtg tctttccggg
     2701 ttggactcaa gacgatagtt accggattag gcgcagcagt cggactgaac ggggggttcg
     2761 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
     2821 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
     2881 gcgcacgagg gagccatcag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2941 ccaccactga tttgagcgtc agattccgtg atgctcgtca ggggggcgga gcctatggaa
     3001 aaacggccgc tgtgcggcct ttccttcttt cctccatgcg attactcggt tttctcgagc
     3061 gtttctacgc atgctcctgc cgctcgccgc agccgaacgg caggagcgta gcgactgagt
     3121 gagcgaggaa gcggaaaaga ggagatccct acactgacca ctgacgctct gaggcctctt
     3181 atgacgaaac aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg
     3241 ctcctgctgg agaagcttaa cgagcttgat ctcgatgtcg aggccgacct ttgtgagaaa
     3301 ctccacgatg atgcggagca tcttttccgt acactgtcct cgagactgga tgcattacag
     3361 gatggtaact gatggccaga gtgtctatta gtgaggctgc ccgactgacg ggtaagagta
     3421 gaacaacact gcacaggtta attaaagcag gtgacctgtc cacctgtagc ggtgagcgca
     3481 atgcaaaaat ggtagatact tcagagcttt tacgtgtctt tggccccttt gagcaaccga
     3541 agggtgaaca ccattctgga caggtctctg gacagcgtga tacagcctct gctgaccaga
     3601 gtgaacaggt gatccgtcat ctgaaacagg agattgaaca cctgaacacc ttacttttgt
     3661 ccaaggattc gcacattgac agcctgaaac aggccatgct ccttcttgaa aatcaccagg
     3721 caaaattaac tgaatccccg gcgccatggt ggaaattctg gaaaaaatcc tgaccccgtc
     3781 atgcttcagt tttcctctcc ggccatccca gggagttcct gaaccttccc aaaaatcgtc
     3841 agatctctga acgctcctca ccactaaaaa tacccacacc ccacgcaaaa ttaaaatttt
     3901 gcagattcct tatttacaac aatgggttaa tgaaaaataa atgtgcggtg atgtgttaca
     3961 aagtgcagat ccaagtcgtg gacgactcta tggcgaatcg ctgacgaatt gcccacgatt
     4021 catatgtgaa tcacgcttta ttatcatatt gttatgatga atcgactctt gattcaattc
     4081 atcactttgg gtgttttttg ctcatggtga ctcttgattc aattcacgat tcgaatcgcc
     4141 ggtgaatcgt gtggtgagtc gtccggcgac ttgccataac gtgattgccg gccgtcgacc
     4201 tgcaggcatg c
//
