LOCUS       NC_011228               6758 bp    DNA     linear   CON 25-JUN-2024
DEFINITION  Escherichia coli plasmid pO26-S4, complete sequence.
ACCESSION   NC_011228
VERSION     NC_011228.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225278
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Fratamico,P.M., Yan,X., Morabito,S., Esposito,G., Needleman,D.,
            Pepe,T., Tozzoli,R., Cortesi,M.L. and Caprioli,A.
  TITLE     The DNA sequence of the virulence plasmid and 5 additional plasmids
            carried by Shiga toxin-producing Escherichia coli O26:H11 strain H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Fratamico,P.M., Yan,X., Morabito,S., Esposito,G., Needleman,D.,
            Pepe,T., Tozzoli,R., Cortesi,M.L. and Caprioli,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2008) Microbial Food Safety Research Unit,
            USDA-ARS, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009952575.2-RS_2024_06_25
            Annotation Date              :: 06/25/2024 01:48:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FJ004638.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H30"
                     /serovar="O26:H11"
                     /db_xref="taxon:562"
                     /plasmid="pO26-S4"
                     /note="Shiga toxin-producing bacterium"
     gene            complement(20..295)
                     /locus_tag="HS602_RS00005"
                     /old_locus_tag="pO26S4_01"
     CDS             complement(20..295)
                     /locus_tag="HS602_RS00005"
                     /old_locus_tag="pO26S4_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091753.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            474..2693
                     /gene="mobQ"
                     /locus_tag="HS602_RS00010"
                     /old_locus_tag="pO26S4_02"
     CDS             474..2693
                     /gene="mobQ"
                     /locus_tag="HS602_RS00010"
                     /old_locus_tag="pO26S4_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_012537771.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RAVDDAVILTPANAPSWCSDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNERALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            2926..4671
                     /gene="col"
                     /locus_tag="HS602_RS00015"
                     /old_locus_tag="pO26S4_03"
     CDS             2926..4671
                     /gene="col"
                     /locus_tag="HS602_RS00015"
                     /old_locus_tag="pO26S4_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_012537772.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNSNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEVSKDPDLSKQ
                     FKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNIRVTTPKRHID
                     IHRGK"
                     /name="col"
     gene            4674..4934
                     /locus_tag="HS602_RS00020"
                     /old_locus_tag="pO26S4_04"
     CDS             4674..4934
                     /locus_tag="HS602_RS00020"
                     /old_locus_tag="pO26S4_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420693.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            4996..5139
                     /locus_tag="HS602_RS00025"
                     /old_locus_tag="pO26S4_05"
     CDS             4996..5139
                     /locus_tag="HS602_RS00025"
                     /old_locus_tag="pO26S4_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            5669..6562
                     /locus_tag="HS602_RS00030"
     CDS             5669..6562
                     /locus_tag="HS602_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277131.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRMGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(6533..>6758)
                     /locus_tag="HS602_RS00035"
     CDS             complement(6533..>6758)
                     /locus_tag="HS602_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_052998717.1"
                     /translation="MHHRTPLRFLAHSLATLGHETAASATTSIMRKATPLRGRTEKIAR
                     KVTAQKDAKRALLSDKAYLVSFCEIWITF"
                     /name="hypothetical protein"
     BGC             1..6758
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(300..473)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(474..832)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5670..6562
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.387"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          475..2690
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.512"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcacttacg cgccggggct tagtggttct gcggtttcgc cggtggtctg ggtagcatct
       61 ccagctcgtt aatcagcggt tgtagtcggt tcacatccgc ctgtcttgtg acttcctttc
      121 gcagaaactg gagcaggaac gcacgcagtt gcgcttcttc cggcctccgt accctcgcca
      181 gcatggcagc ccccacaatg actttttgcg ccgtgtccag gctccggctc ttcgccttca
      241 ggcgctgtaa tctggcctca gcttcggcaa tcttctgttc gagtgttctg ctcatttcgt
      301 gactccgtgc gcggtgaaaa atcgcatttt agcgcgtcac tggtagttgc aaaactgaac
      361 tggcataatg cacggcacat cacgaagtgc gcacttatac aatctctact ccgtttcgat
      421 tgtgtgcggt ctgcgacgct aaaagaaaac ggcaaaaagg tattacggca gaaatggcga
      481 tttatcatct cagcatgaaa atcatttcgc gaaaaaacgg ctacagtgcc gttgcttctg
      541 ctgcctaccg ttccggctct gtcatacccg atgaccgtac cggattaacc cacgattaca
      601 cccgtaaacg cgccgttgat gatgcggtca ttctcacccc tgcgaatgca ccgtcctggt
      661 gtagtgaccg ttccgttctc tggaatgcgg tcgagaaagc cgaacagcgc cggaactccc
      721 agctggcaag ggagattgaa ctcgccattc cccgtgagat ttcccgcgag gccgcacggg
      781 aggccgttct cgcttttgtc cgggaaaact ttgtcagtcg gggcatgatt gccgatgtgg
      841 cgtttcatca catggaccgg accaatcccc atgcgcacat catgctgacc acgagagctg
      901 tcggggaaac aggatttgca ggaaaggtca gggactggaa cgagcgggca ctcgccgaga
      961 cgtggcgcgc atcatgggct gaccatgcga acagagcgct tgcgaacgcc ggctaccagg
     1021 aagagataga ccatcgttca tacgagcgtc agggactgga gaaagcgccg ggccttcacc
     1081 tcggaaaggc tgcctgtgcg atggaaaaac ggggcatgga aacagaacgc ggtgagcaga
     1141 accgcctgat taacagcctt aacctggaaa tacaggtttc ccgcacaagg ctggcagtca
     1201 tccaggtaca ggagaacgtg cggaaacggg aactcagcga tgccgcacgt cgtgcagcgg
     1261 aagcccttaa cctgaccatt cccgctgcga atgcctcagc ggataccctg cgggaattca
     1321 ttgccacgct gccgcaggaa tgcgggaacg cgtgggagat gaccccggag ttcctggcga
     1381 tgagcgggaa ggtgaacgac atcgaacgtg aggggaatgc gctgctgaaa gagcaggcca
     1441 ttctcgaaaa ggagatgacc ggactcaaaa aagcacgccc tgtcgcgtcc cttctgtcag
     1501 agattcccct gatgacatgg gctgaaccgg aataccgcaa aagacaactc cgtttctgga
     1561 aactcgggaa acagattgaa tctcttcgcc gcacctacag ggccgtgaaa gaacgggaca
     1621 ttcccgcccg tcgtcaggcc tttgaaacgc agtggaatac gtggattgcg ccgggaatgg
     1681 cagagctgaa agaaaaactg tcagcacggg aagcggagcg gcgcagggag gaagccgaag
     1741 cggaagcgcg ccggaaggaa caggagcatg aggcgcggct gaaacgtcat gataaccacc
     1801 gtctgagccg tgaaacggca ttagtcgggg ttattacgga gctggggcgt gccagagagc
     1861 cgggaacggg caggataacc cgctacatga tgctgagtaa cagagccgga gaattcacgg
     1921 tatggggtga tgagctggcg cattaccccc agagtgttca tgacccggtg aatgtttacc
     1981 tgtcgccagg cggggctgtg atggtctcgg atatacgtga gggaatgcca gaatctcatg
     2041 agacgatggc gcggcctgag cgtgtgagaa tgtattccgg tgcgacggtc cggcatgtac
     2101 tggaacagat gcgccagggg tggccctctt acggttttcc ggcgctgccg catcactggc
     2161 cggataattt ttatttcagc gacgaccgca ggcccgtagc ctctccgctg ccgtcagcgc
     2221 accgggtgga tatcaccgct gatgctgtac cggaacaact catgcctgtt gtattttcga
     2281 gagagggaaa cagcaggacg ctgaatctgt tgttatgcaa agggccggag gaaatgcttg
     2341 tcgggtttgt tcgccgggaa ggagggtttt gccccgttct tgtgcttccg tcgccggatt
     2401 acagtcatct gatagtcagc accatcacgg agaacggggt atgcctggca ggttacggag
     2461 aagctataaa ccatgatgag gatactccgt acccaccgga accgcacctg atgcagttcc
     2521 ggctcaaagg ccatcatgac aggcttttgg ctgctgtcca caaaccggaa gagatgccgg
     2581 attatctctt ccgtcaactc ggttttaatc agacctggca tgagtggaag cgggacgaac
     2641 agcacaggca acaacaacgc cgccccgggc acttccgggg catgagtatg tgatatccgg
     2701 ggctgccccc cggaccccgc caacacatca cgggccacaa aattttttgt ggcccgctct
     2761 gcgttttcta agtgttatcc ctcctgattt ctaaaaaatt ttccacctga acttgacaga
     2821 aaaaacgatg acgggtactt tttgatctgt gcataaaacc agtggtttta tgtacagtat
     2881 tgatcatgta attaattgtt ttaacgctta aaagagggag tttttatgag cggtggcgat
     2941 ggacgcggcc ataacacggg cgcgcatagc acaagtggta acattaatgg tggcccgacc
     3001 gggcttggtg taggtggtgg tgcttctgat ggttccgggt ggagttcgga aaataacccg
     3061 tggggtggtg gttccggtag cggcattcac tggggtggtg gttccggtca tggtaatggc
     3121 ggggggaata gtaattccgg tggtggctcg ggaacaggcg gtaatctgtc agcagtagct
     3181 gcgccagtgg catttggttt tccggcactt tccactccag gagctggcgg tctggcggtc
     3241 agtatttcag cgggagcatt atcggcagct attgctgata ttatggctgc cctgaaagga
     3301 ccgtttaaat ttggtctttg gggggtggct ttatatggtg tattgccatc acaaatagcg
     3361 aaagatgacc ccaatatgat gtcaaagatt gtgacgtcat tacccgcaga tgatattact
     3421 gaatcacctg tcagttcatt acctctcgat aaggcaacag taaacgtaaa tgttcgtgtt
     3481 gttgatgatg taaaagacga acgacagaat atttcggttg tttcaggtgt tccgatgagt
     3541 gttccggtgg ttgatgcaaa acctaccgaa cgtccaggtg tttttacggc atcaattcca
     3601 ggtgcacctg ttctgaatat ttcagttaat aacagtacgc cagcagtaca gacattaagc
     3661 ccaggtgtta caaataatac tgataaggat gttcgcccgg caggatttac tcagggtggt
     3721 aataccaggg atgcagttat tcgattcccg aaggacagcg gtcataatgc cgtatatgtt
     3781 tcagtgagtg atgttcttag ccctgaccag gtaaaacaac gtcaggatga agaaaatcgc
     3841 cgtcagcagg aatgggatgc tacgcatccg gttgaagcgg ctgagcgaaa ttatgaacgc
     3901 gcgcgtgcag agctgaatca ggcaaatgaa gatgttgcca gaaatcagga gcgacaggct
     3961 aaagctgttc aggtttataa ttcgcgtaaa agcgaacttg atgcagcgaa taaaactctt
     4021 gctgatgcaa tagctgaaat aaaacaattt gatcgatttg cccatgaccc aatggctggc
     4081 ggtcacagaa tgtggcaaat ggccgggctt aaagcccagc gggcgcagac ggatgtaaat
     4141 aataagcagg ctgcatttga tgctgctgca aaagagaagt cagatgctga tgctgcatta
     4201 agtgccgcgc aggagcgccg caaacagaag gaaaataaag aaaaggacgc taaggataaa
     4261 ttagataagg agagtaaacg gaataagcca gggaaggcga caggtaaagg taaaccagtt
     4321 ggtgataaat ggctggatga tgcaggtaaa gattcaggag cgccaattcc agatcgcatt
     4381 gctgataagt tgcgtgataa agaatttaaa aactttgacg atttccggaa gaaattctgg
     4441 gaagaagtgt caaaagatcc cgatcttagt aagcaattta aaggcagtaa taagacgaac
     4501 attcaaaagg gaaaagcacc ttttgcaagg aagaaagacc aagtaggtgg tagggaacgc
     4561 tttgaattac atcatgataa accaatcagt caggatggtg gtgtctatga tatgaataat
     4621 atcagagtga ccacacctaa gcgacatatt gatattcatc ggggtaagta aaaatggaac
     4681 tgaaacatag tattagtgat tataccgagg ctgaatttct ggagtttgta aaaaaaatat
     4741 gtagagctga aggtgctact gaagaggatg acaataaatt agtgagagag tttgagcgat
     4801 taactgagca cccagatggt tcagatctga tttattatcc tcgcgatgac agggaagata
     4861 gtcctgaagg gattgtcaag gaaattaaag aatggcgagc tgctaacggt aagtcaggat
     4921 ttaaacaggg ctgaaatatg aatgccggtt gtttatggat gaatggctgg cattctttca
     4981 caacaaggag tcgttatgaa aaaaataaca gggattattt tattgcttct tgcagtcatt
     5041 attctgtctg catgtcaggc aaactatatc cgggatgttc agggcgggac cgtatctccg
     5101 tcatcaacag ctgaagtgac cggattagca acgcagtaac ccgaaatcct ctttgacaaa
     5161 aacaaagcgt gtcaggctga ttctgatgcg cttttttttt gaaatgtcac aaaaattcca
     5221 tgtgggagat gggatctaaa atccccgtgc agaactttcc atccaggggg agaaaacttg
     5281 tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga tcgcgcatcg ctttcgtgca
     5341 tagttatgca acgcgctaaa aacgattctg acgcattttt tatgtttacc ctggtgtttc
     5401 cttgtctttt tgcgtttttt gcgtcagaaa gcgattgagg gcgttttaag gggggcgtac
     5461 aacgggagtt atggtaaatg gatcgggttt tcgggaagga tcgacaggat ttgccgttgg
     5521 gtgtagtgta agcgactgaa aaacaaacgc cccgtaaatc gtgctctcac cgccaagatt
     5581 gatcacgaaa ttacagggcg ccgggttccg cgtttcccga tgggaaagcg cggttagtta
     5641 aactgtgtac cgagagaaat cgtatcacat gagcgccgta cttcaacgct tcagggaaaa
     5701 attaccgcac aaaccgtact gtacgaacga tttcgcgtac ggcgttcgca ttctgccgaa
     5761 aaacattgcc attcttgccc gtttcatcca gcagaaccag ccacatgcac tgtactggct
     5821 tccctttgac gtggaccgga tgggggcatc aatcgactgg agcgaccgga attgtccggc
     5881 cccgaacatc accgtaaaaa atccccgtaa cgggcacgcg catctgctct acgcgctcgc
     5941 ccttcctgtg agaactgcgc cggatgcatc ggcttcggcg ctcagatacg ctgccgctat
     6001 tgagcgtgcg ttgtgtgaaa aactgggcgc ggatgtgaat tacagcggcc tgatctgcaa
     6061 aaatccgtgc caccctgaat ggcaggaagt ggaatggcgc gaggaaccct acactctcga
     6121 cgaactggct gattatctcg atttgagcgc ctcagcgcgc cgtagcgtcg ataaaaatta
     6181 cgggctgggg cgaaactacc atctgttcga aaaggtccgt aaatgggcct acagagcgat
     6241 tcgtcagggc tggcctgtat tctcacaatg gcttgatgcc gtgatccagc gtgtcgaaat
     6301 gtacaacgca tcgcttcccg ttccgctttc tccggctgaa tgtcgggcta ttggcaagag
     6361 cattgcgaaa tatacacaca ggaaattctc accagaggga ttttccgctg tacaggccgc
     6421 tcgaggtcgc aagggcggaa ctaaatctaa gcgcgcagca gttcctacat cagcacgttc
     6481 gctgaagccg tgggaggcat tagggatcag tcgagcgacg tactaccgaa aattaaaatg
     6541 tgatccagat ctcgcaaaat gagaccagat aagccttatc agataacagc gcccttttgg
     6601 cgtctttttg agcagtgacc tttcttgcga ttttctccgt tctgccccgt aatggcgtgg
     6661 ctttccgcat gattgaggtg gtagcgctcg ccgcagtctc atgaccgagc gtagcgagcg
     6721 aatgagcgag gaagcgcaaa ggcgtccggt ggtgcatg
//
