LOCUS       NC_011227               4073 bp    DNA     linear   CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pO26-S3, complete sequence.
ACCESSION   NC_011227
VERSION     NC_011227.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225279
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Fratamico,P.M., Yan,X., Morabito,S., Esposito,G., Needleman,D.,
            Pepe,T., Tozzoli,R., Cortesi,M.L. and Caprioli,A.
  TITLE     The DNA sequence of the virulence plasmid and 5 additional plasmids
            carried by Shiga toxin-producing Escherichia coli O26:H11 strain H30
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Yan,X., Fratamico,P.M. and Needleman,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-AUG-2008) Microbial Food Safety Research Unit,
            USDA-ARS, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009952565.2-RS_2024_06_24
            Annotation Date              :: 06/24/2024 03:00:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FJ004637.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="H30"
                     /serovar="O26:H11"
                     /db_xref="taxon:562"
                     /plasmid="pO26-S3"
                     /note="Shiga toxin-producing bacterium"
     gene            468..581
                     /locus_tag="HS546_RS00020"
     CDS             468..581
                     /locus_tag="HS546_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531573.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGN"
                     /name="replication initiation protein"
     gene            792..1721
                     /locus_tag="HS546_RS00005"
                     /old_locus_tag="pO26S3_02"
     CDS             792..1721
                     /locus_tag="HS546_RS00005"
                     /old_locus_tag="pO26S3_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000571803.1"
                     /translation="MIEMDMPNTIHYPHVIPFISQGKINAIKSTFGNNLSDRECYGIYI
                     WSQKASSAIYPLLQQLEVTLRNSIDKEATKLIGQKWWDNVYTDTSKSKHGDFIHNINKA
                     KRRYENEFKKKNPSMANTKIIAPHDDIIAHTDFYTWQAVLSDAFHTQSRSEASRALWPR
                     LTYRVLKGLDRSKDEGTARIDFLNELNEIRNYRNRLSHNDCIWIKIHSKNLQSAVETIR
                     EKINKIEGLIKTINPQVHLSLTKWGSFYHAKRICSQKEAELHLGKGIINSTTDEMNTIL
                     DQLYALTADGKLTGVVKRNTNNIAFHKF"
                     /name="Abi family protein"
     gene            2315..2908
                     /locus_tag="HS546_RS00010"
                     /old_locus_tag="pO26S3_03"
     CDS             2315..2908
                     /locus_tag="HS546_RS00010"
                     /old_locus_tag="pO26S3_03"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012537770.1"
                     /translation="MSGRKFGRLPNWWARSSWLVNNVRSNEIGGGIASMKCLLALSVLI
                     DFHSRTASVSFTGMERLTGLSRPMIVKGVAKLEKDGLIKIDREMFVNSYELTVQPNDDR
                     WAKVPVDTIRKKLNTISNRGMAPFVALKLYLTIISLRYQSNNTVKVTHETLRSYTGVQP
                     NQIRFGLDVLYCSRLIHLQPKEDRESNIYEIIGL"
                     /name="hypothetical protein"
     gene            3681..3875
                     /locus_tag="HS546_RS00015"
     CDS             3681..3875
                     /locus_tag="HS546_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3167..3422)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.411"
                     /coverage="89.0"
                     /evalue="2.67e-103"
     Typing          complement(3547..3645)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(3957..4043)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          2..540
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.387"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 atgccgcaca gatgcgtaag gagaaaatgc acgtccaggc gcttttccgc ttcctcgctc
       61 actgactcgc tacgctcggt cgttcggctg cggcgagcgg tactgactca cacaaaaacg
      121 gtaacacagt tatccacaga atcaggggat aagaccggaa agaacatgtg agcaacaagg
      181 caaagccagg acaaaaggcc gcgccgctgg cgtttttcca taggctccgc ccccctgacg
      241 agcatcacaa aaaccgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      301 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      361 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      421 gttggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      481 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      541 ggcacgcctt aacgccactg gcagcagcca ctggtaactg atttagagga gtttgtcttg
      601 aagttatgca cctgttaagg ctaaactgaa agaacagatt ttggtgactg cgctcctcca
      661 agccagttac cttggttcac agagcgccaa ctctttgaac cttcgaaaaa ccgcccgcca
      721 aggcggtttt ttcgtttaac actctacaac tatccatacg tgtatggcaa catccaatca
      781 ccactaagtg tatgatagag atggatatgc caaatacaat tcattaccct catgtaatac
      841 catttatatc ccaaggaaaa ataaatgcta taaaaagcac gtttggtaat aacctgtctg
      901 atagggagtg ctacgggata tatatatgga gccagaaagc ctcatctgcc atatatccat
      961 tactgcaaca actcgaagtt actttgagaa actcgattga taaagaagcg acaaaactaa
     1021 taggccaaaa atggtgggat aatgtataca ccgacacatc aaaaagcaaa cacggtgact
     1081 tcatacataa tataaacaaa gcaaaaagaa gatatgagaa tgaattcaag aaaaaaaacc
     1141 catcaatggc aaataccaaa ataattgcac ctcatgatga catcattgca cacacagatt
     1201 tttacacatg gcaagcggtt ttgagcgatg cttttcatac acaatcaaga tctgaagcat
     1261 caagagcctt atggcccagg ctcacctaca gagtactgaa gggattagat agaagcaaag
     1321 atgaaggcac agccagaatt gatttcttaa acgagctgaa tgagatcaga aactacagaa
     1381 ataggttatc gcataatgat tgcatatgga taaaaataca ctctaaaaat ttgcaatctg
     1441 cagtagaaac aataagagaa aaaataaaca aaatagaagg actgataaaa acgataaacc
     1501 cacaggtaca cctatcatta acgaagtggg gttcctttta tcacgccaaa agaatatgtt
     1561 ctcaaaaaga ggccgaattg catctaggca aaggaataat caatagcaca acagatgaga
     1621 tgaacacaat tctagatcaa ctatacgcac taactgctga cggaaagcta acgggtgttg
     1681 taaaacgaaa taccaacaat atcgcatttc acaagtttta gatcacaaaa tagaaaaggc
     1741 ccgacagttt cctgtcgagc ctcatccaaa tctcttgcca ttatcacctt tcggtggagc
     1801 gtggccttaa tgtcattgtg aatctataaa aatttcacaa tgcattaaag cgaaagctca
     1861 aggcttcact gccatacatg atacatcatc atcaggaaaa agagcaaact atttcccatg
     1921 atcacgccaa agcttaccat ccaccattta ccaatagatc aaaaaacaca caacaaaaat
     1981 aactaaaaac gccgattaaa ccagcattat caaatttaca tttaccagtt aaaaaacgat
     2041 aaaaacaaga taacatcatg ttttatatgg gaaaacaagc agccaccaaa tattgcttta
     2101 tctagattta ttctaataag cgtatataaa gttaaaacac aacaacttag tatgcgactg
     2161 gaggaccaat tggaaaccct caacaatttg ctggtaacgc taacttccgc aaagccggag
     2221 gttttggttt ctgttgtatc tttaggtgca atggttttca tgtatatgat aataaagcta
     2281 tttatatctc tccttgaccg aaaatggggt aaaaatgagc ggtagaaaat ttggcaggct
     2341 tccaaactgg tgggcccgaa gctcatggct tgtaaacaat gttcgttcaa atgagatagg
     2401 gggcggcatt gcatcaatga aatgcttgtt agctctatcc gtcttgattg atttccattc
     2461 aagaacagcc tcagtatcat ttaccggaat ggaaagactg actggactaa gccgccccat
     2521 gatagtgaaa ggagtagcca aacttgagaa agatggtctt ataaagatag atagagagat
     2581 gtttgttaac tcctatgaat taacagtaca gccaaatgat gataggtggg caaaagtacc
     2641 agtagatact atcaggaaaa aactaaacac catatcgaac agggggatgg ccccatttgt
     2701 ggcattaaaa ctatatttaa caattataag ccttcgttat cagtcaaata atactgttaa
     2761 ggtgacacat gaaacactga gaagctatac aggtgtccag ccaaaccaga ttaggttcgg
     2821 cttagatgta ctgtactgta gtcgcctgat acatctgcaa ccaaaagaag atagagagtc
     2881 aaacatctat gaaataatcg gcctatgagg aaatgcattt accataacgt cgcattacgc
     2941 gcaccgctaa aacgcactga acgcgattct gacgcagata ccgcaaaaag agatgtttta
     3001 cccagtgaaa accgaaaaaa tgcgtcagga tcgttttcag cccactgcat agctatgcat
     3061 gaaaatgaat gacgaacggt ttgggcgcat acggtgccca tgagacacat tttcggacat
     3121 tttcagttcg tgaatccctg actcattgct acatatctga acagacaccc ctggcagcgc
     3181 ccccaggggc gtcctgtaaa aaaacacacc gcgccgctag cagcacccct gatataaaat
     3241 gatgtttttt ataaaaatag tcagtaccac ccctacaaag cggtgtcggc gcggtgttgt
     3301 agctgcgccg gcaccgcttt tttaaatatc gcaaagagag caagagaaac taatttttca
     3361 taactctcta tttataaaga aaaatcagca aaaacttgtt tttgcgtggg gtgtgggcat
     3421 ttcaggaatg agaacagact cgcgtttttc cggagtatca gcggaattat tgattaagaa
     3481 acactcgacg cagagcgaaa ttttatgcaa cagcggctgc actgcacagg gattatttat
     3541 cagttgatat gctgtgccat gatacgtcct aaaaaaacag gacaggatgt gcaattgtaa
     3601 taccgtcgcc cgcaacgtct tacaattacc agctgctcag ggtccccccg acacccggaa
     3661 ctgacggaga acaacggaag gtgaacaaac aacaacaaac tgcgctgaat atggcgcgat
     3721 ttatcaaaag ccagagcctg acactgcttg aaaaactgga tgctctggat gccgacgaac
     3781 aggcagccat gtgcgaacga ctgcacgaac tcgcggaaga actccagaac agcatccagg
     3841 ttcgctttga gaccgaaagt gaaacaggaa cataacgaag ctgccgaaaa cgctcacggc
     3901 ttgtccgcga gcgttttcgg cagcagacaa gcccgtcagg gcgcgtcagg gggttttagc
     3961 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcggc atcagtgcgg attgtatgaa aagtgcccca tgccgggtgt gaa
//
