LOCUS       NC_011073               5594 bp    DNA     circular CON 23-OCT-2023
DEFINITION  Bacteroides fragilis plasmid pBFP35, complete sequence.
ACCESSION   NC_011073
VERSION     NC_011073.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225685
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Soki,J., Wareham,D.W., Ratkai,C., Aduse-Opoku,J., Urban,E. and
            Nagy,E.
  TITLE     Prevalence, nucleotide sequence and expression studies of two
            proteins of a 5.6kb, class III, Bacteroides plasmid frequently found
            in clinical isolates from European countries
  JOURNAL   Plasmid 63 (2), 86-97 (2010)
   PUBMED   20026106
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Soki,J., Ratkai,C. and Nagy,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JUL-2008) Institute of Clinical Microbiology,
            University of Szeged, Somogyi Bela ter 1., Szeged H-6725, Hungary
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 5594)
  AUTHORS   Soki,J., Ratkai,C. and Nagy,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JUL-2006) Institute of Clinical Microbiology,
            University of Szeged, Somogyi Bela ter 1., Szeged H-6725, Hungary
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 10/23/2023 01:27:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            DQ859906.2.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="P35"
                     /db_xref="taxon:817"
                     /plasmid="pBFP35"
                     /note="clinical isolate"
     gene            join(5328..5594,1..504)
                     /locus_tag="HS421_RS00005"
                     /old_locus_tag="pBFP35_p1"
     CDS             join(5328..5594,1..504)
                     /locus_tag="HS421_RS00005"
                     /old_locus_tag="pBFP35_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799241.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            complement(574..1824)
                     /locus_tag="HS421_RS00010"
                     /old_locus_tag="pBFP35_p2"
     CDS             complement(574..1824)
                     /locus_tag="HS421_RS00010"
                     /old_locus_tag="pBFP35_p2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBU/pMV158-type mobilization protein"
                     /protein_id="WP_005799237.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="NBU/pMV158-type mobilization protein"
     gene            2230..2478
                     /locus_tag="HS421_RS00015"
                     /old_locus_tag="pBFP35_p3"
     CDS             2230..2478
                     /locus_tag="HS421_RS00015"
                     /old_locus_tag="pBFP35_p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_005812783.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            2472..2792
                     /locus_tag="HS421_RS00020"
                     /old_locus_tag="pBFP35_p4"
     CDS             2472..2792
                     /locus_tag="HS421_RS00020"
                     /old_locus_tag="pBFP35_p4"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_005799262.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            2829..3227
                     /locus_tag="HS421_RS00025"
                     /old_locus_tag="pBFP35_p5"
     CDS             2829..3227
                     /locus_tag="HS421_RS00025"
                     /old_locus_tag="pBFP35_p5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799260.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="hypothetical protein"
     gene            3722..4771
                     /locus_tag="HS421_RS00030"
                     /old_locus_tag="pBFP35_p7"
     CDS             3722..4771
                     /locus_tag="HS421_RS00030"
                     /old_locus_tag="pBFP35_p7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_005799258.1"
                     /translation="MCKDRTNITNCKESPLKNGSDSLKKKAKVKIISHNFTRKLSEKNP
                     DSKLMKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKKFLPVMKYLAS
                     EGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGISGVIKKECTY
                     HVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKKIEDFENAAIE
                     VFKYASKASVSKSKGRDGKKKIQINYKALDMIYTAMHGMQRMSSFGQFRTMIGDEALND
                     FRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTRKDELLQDIYL
                     DEDIFPNSG"
                     /name="protein rep"
     gene            complement(4775..5236)
                     /locus_tag="HS421_RS00035"
                     /old_locus_tag="pBFP35_p8"
     CDS             complement(4775..5236)
                     /locus_tag="HS421_RS00035"
                     /old_locus_tag="pBFP35_p8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005812785.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     Typing          3723..4771
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(578..1824)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgcaggttt taaatgctca agtggcaagt gctatttcaa atcctaatac cgcttatgtt
       61 gttttttctg taaatggaaa gactttggtt aaaaaggtga aggaggatgc taattttgat
      121 ttttcagtat ttagggatgt tgtaacggaa acgagggctg ttctcccttc tttgagtatt
      181 aatggaggtt ctcaaagcac taccggagtt ttttacgatt ccagtcgtac tttaaagatg
      241 caagttgatt tgaatgcttc aatacagaat aattattatt tctttgaagt attaaatcct
      301 aatgctaaac caagtccgga tgataatata acgacacctg aaagtgttgc ttttagtgga
      361 actggaccgt tatggagtaa cactttcact tggacttcgt attgggatgc aaatgtacct
      421 ggtcaaggtt ttaaatggga gtttaaagga aaaggtacta ctccttcttt tggttttata
      481 gctaattgca cctttagtcg ttaaaaaatg aacgggattt aatttgaatt gcacttctca
      541 aaaaagagaa gtgcaattct ttttagtgtc tttctatcgg tgtagccagt taccgttatc
      601 taaagtcttt tgtagtgtat tccaaagttc tttaagaaag tttttaaagt ggatgttatt
      661 tagccaaatt gtagtaatac cattctgact tttggagata tctattttca cgtttttaac
      721 ttggtatgat tttctcttgt ctttatcgta aaggtttcca gtgtagtata tttcctttcc
      781 aataagaagt tgtcggatat ctttattttg tattcccata gtttggagtt cctctatatt
      841 ttcctgtgca ttttccattt ccggaaaaag gttggttatg ggttttagtt tggatgcttc
      901 tgcctttatc tgtctgtcgg ttgtttcctg tacccttcgc atctcgatgt tgttggttct
      961 taattgtcct tctaaatttt gattcttgaa tgagaggtta taattttctt ttttgagggt
     1021 ttcaatttct ttggcttgtc gttttacttc ccctacatta aagacgcttg taacggcatt
     1081 aaatacggtt tttccggctt ttgcggcagc ttctttggct tcgttgatgt gtatgtcggt
     1141 tttcatgctg tttagctctt tttgtgccgt actaagttcc ttttggactg tggtgatttt
     1201 ctcggttccg gcagtgtatg ccctgtttag ttgctcgatg tctttttgaa gttgttcagc
     1261 tttctctgtt tccattttgt ttttgtattg catggccgac aagtgtttct tgtctgaaga
     1321 aactccacgt tccatgttga gacactctgc tgttatagtc tgcatctccg ccatgtcgtg
     1381 tctgttcagc tttacagttt ttcctgtatg gccgtccgtc cagtcaaaga tcatgtgcgc
     1441 atgatagttc ggtttccatg cttcttcctt tccgtcccat acgtttgcgc cttcatcctt
     1501 atgggtgtag atttggaagg cgtgcactcc aaagcgttct tccagttttt cggccaagtc
     1561 ttgaagctgc tggattgtgg tttcctcgct gattactatt accccttccc ggattggggt
     1621 agctttcttc tgtaatcctt gtccggtggc ttccttgtat ttttccgtta tgtcccggtg
     1681 tacttcggca atggaacatt ctacccactg ttcgttgcgg tgtgtgagtt ccggacggat
     1741 gtagtcgagc tccttgcttc ttaagttgtg acgttccgag ctgcctatat tgcaggcttc
     1801 cactctgata catgtctttg ccatatcttt aggttttagg tgtgtatgtc tgtttagaag
     1861 atgtggcgtg aaacgtcaca tcgcacgggg tcgaggggac acccctcgca cacaacccct
     1921 ttaggggtta tagtgtgcta taaccttttg caaaggtaag ccttccggac gggatttgca
     1981 agcgctctgc catgtgttcc ggatgtgctt gggaacagaa gccggacaaa agggaaaagg
     2041 acacaaggca caataaaaaa tcatcggtcg gggagcgctg cacggcaagg gggtgcacca
     2101 acactcccga cgagtgcagc cattgacagg gttctcggtg aaaatggaaa aaagttgttt
     2161 ttccttttgt tattgttata actttacata tatttgtatc gtgataatca attataaagt
     2221 tataacatta tggtaacgaa tattattcag attggaaaca gcaaggggat aatacttcct
     2281 tccgaagtat tgaaacagtt gcgtttgtcc ttgaaatcgg ctgtgagcat ttctttggac
     2341 gggaacaata ttgtgataaa ggcacaacca cggcagggat gggcggaagc tgccaaacgt
     2401 gcccatgaga atggggatga tgagttgctt atccctgatg tgtttgagga tgaaaagttt
     2461 gaggattgga catggtagaa cagtatgaag tgtattgggt agagttagac cctacccgtg
     2521 ggggtgaaat ggcaaagaca cggccatgtg tagttgtcac tccttcagat ttgaatatgt
     2581 accttacaac agtagtcatt gttccgataa cctcaacgat aagaaattat ccttaccggg
     2641 ttctgtgttc tgtagccggg cgtgaaggag agatagccac tgatcaaatt cgcacggtgg
     2701 ataaaagccg gctgaaaaga aagattggag atttgaattt cagtgagata aaaaggttac
     2761 gtgaagtgtt tcagcaaatg ttctgtgaat aatggaaagt acggaaagaa aaatgcaaat
     2821 aagacattat gaaaccaact gactacatcg aatgggataa cctgaaggat attccattct
     2881 tcctctgtca agtagtcgag gacagagaaa aacaagattt ggatatttac tatttgggca
     2941 aacgtgtctt gcacgattac gaccatgtgg ggcactattt gcggactgcc gttatcttgt
     3001 tccgcagggt aaagagccgc acagcagact gggtaaacct gcgtaacctt tggacgctgc
     3061 gcaactgtgt gcgtgagaac tacaatcacg gcataggaat gaacgacctt atttttgggg
     3121 agaatttcga tggagataat ctcgatacgc ttacaccact tacaaagaaa cgctttgatt
     3181 ttttgtgtaa gcggattaag gaacttgacc catatgcaac tatttaacac tccattgagt
     3241 gaaggcaggg tttgtctttg tgtgctcccc ctttggggga cgggaggggg ataagaaaaa
     3301 aaataccacc ggagatgtcg gatatccgtc aattcactcc ggtgtttttt ttgcaactct
     3361 cccgtatcgc tcggcaaggg ttgtctttgt gtacggcaaa gatagttttt tttccggaag
     3421 tatctttgtc tgttgtggat ttagtagtgt atttgtgtca gaattgcgca gtcgggggaa
     3481 tgggggcgag cccccatact agataaacta gaaagtttcg tggtaaatgc tttttgactt
     3541 gttatatgta ggttttttga atgatgtttg gcggtttgga atgaattacc tacttttggg
     3601 ctgagaaaaa acgaaagccc gaaggcagta accaacgggc tttgtatttt aagaattagc
     3661 aatcgtttgt gttcgttctt ggcggggcgg ataccaaacg aaagccacat caaaatttca
     3721 tatgtgcaaa gatagaacaa atataaccaa ctgcaaagaa agtccgttaa aaaacggttc
     3781 tgatagtctt aaaaaaaagg ctaaagtaaa aatcatatcc cataatttta ctcgaaaact
     3841 ttcggaaaag aatccagata gtaagctgat gaaagcatat atggatgccc atgtgtgttc
     3901 aaatgtttat actgtgcagg gaggtaaggc gcattcccat tattgtcatc gggctttttg
     3961 tcctatttgt cagcgtattc aaacggcaca aaatgtaaag aagttccttc cggttatgaa
     4021 gtatttggct tcggaaggca gagagtttta ttttgtgacg cttaccttgc aaaattgtgt
     4081 aaccgatgat gcaagggtat tgcgagattt catgcgtaga tgtaaccgga tgtgggctga
     4141 tggtataagg acgaaacaca agtttcgttc acttggtatt tcgggggtga ttaagaagga
     4201 gtgtacctat catgtagtaa aaaaggattt gttttcgttc cattatcatt ttcatattat
     4261 tgttgattca ttggaagctg caaaatatgt agtagcccaa tggaagaaat tgcatggtag
     4321 tacggttgct gatgcccgtt ttcagaaata taagaagata gaggattttg aaaatgcagc
     4381 gatagaggtt tttaagtatg cttcaaaggc ttctgtctca aaatcgaaag gtcgtgacgg
     4441 taaaaaaaag attcagataa attataaggc tttggatatg atttatactg ccatgcatgg
     4501 gatgcagcgc atgagtagtt tcggacagtt ccgcaccatg atcggagatg aagcgttgaa
     4561 tgattttaga gatgaggatt tggttcttaa tattgaggga ttgaatgttg aagatggttg
     4621 ttatacttgg tatatgtctg tgaaagataa ggtggctgat tggtataata tggaaactgg
     4681 ggaggcattg tgttgttata aggtcacccg gaaggatgaa cttttgcagg atatttattt
     4741 agatgaggat atttttccta atagtgggta gttgttagtt tgttgtattg ttttcttggt
     4801 atggtgtgag ttctatgtat tctctattca tgaaaggatc gccaaatttt ccacttctgt
     4861 catagaagga aaatttgttt ttagttagct cgtctatgtc ccagtaatgt tctccaccaa
     4921 aataaatggt actgaaactt ggatcaccga atgaccattg tccgtagata ttggcttctt
     4981 ttttatttcc ttgtttgtcg atttctatat ttgtacgggt atatgttccg tttttattga
     5041 atttgtatgt accttgccag acactgccgt tttcgtctgt tgttttttta ccagcccatg
     5101 taatatcaca aattaatgag attatgcttt cttctccatg gttgtagtat ccttgttttt
     5161 caacacatcc ggtgtttgaa ataagaaaaa gtgatactgt tgaaataagt aaagagatat
     5221 gtattattct attcatagtt aaatatttaa tattcaatgc aaaaatagga gaaattttat
     5281 atttttgggc gaaaccttta tttattaact ttaaaaaagg aggatttatg aaaaaagtaa
     5341 tgtttatgtt catcagtcta ctgactgttt tgggtgtttg ttcgtgtaat gataatgaag
     5401 taatagaacc tgtggttagt gattctgtat ctgatatgga ggtcttatct cgttttgttg
     5461 atgtaaatga gattacaaat gaatactatt tcaatgagaa taagaaaacg agggcgttgt
     5521 cttatgtaac aggttcggat tggcaggatt tagaaaaagt tagtcctttg agtattgaga
     5581 aatataagaa taac
//
