LOCUS       NC_010983               3869 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Alpha proteobacterium endosymbiont of Amoeba proteus plasmid pAP3.9,
            complete sequence.
ACCESSION   NC_010983
VERSION     NC_010983.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225299
KEYWORDS    RefSeq.
SOURCE      alpha proteobacterium endosymbiont of Amoeba proteus
  ORGANISM  alpha proteobacterium endosymbiont of Amoeba proteus
            Bacteria; Pseudomonadota; Alphaproteobacteria.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Park,M., Kim,M.S., Lee,K.M., Hwang,S.Y. and Ahn,T.I.
  TITLE     Characterization of a cryptic plasmid from an alpha-proteobacterial
            endosymbiont of Amoeba proteus
  JOURNAL   Plasmid 61 (1), 78-87 (2009)
   PUBMED   18951917
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Kim,M.S., Park,M., Lee,K.M., Hwang,S.Y. and Ahn,T.I.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2008) Department of Biological Sciences, Seoul
            National University, San 56-1 Sillim-Dong, Kwanak-Gu, Seoul 151-742,
            Korea
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009952375.1-RS_2024_03_14
            Annotation Date              :: 03/14/2024 02:52:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            EU727537.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="alpha proteobacterium endosymbiont of Amoeba
                     proteus"
                     /mol_type="genomic DNA"
                     /host="Amoeba proteus"
                     /db_xref="taxon:538945"
                     /plasmid="pAP3.9"
     gene            661..1941
                     /locus_tag="HS527_RS00005"
                     /old_locus_tag="pAP3-9_01"
     CDS             661..1941
                     /locus_tag="HS527_RS00005"
                     /old_locus_tag="pAP3-9_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063008.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_000063008.1"
                     /translation="MSMSVSLKKATNKTNIRHNNRTMSEKEKEQNSHIDYSRSDENKYL
                     VQKDLKKLYREEFGEVLENYNAKQKRNDRKIDDYYKHIQSSKKTSLQQEMIIQVGDKDD
                     FSSKQDFEKANEILEEWLLGFEKRNPNLKIYNAVIHNDEASPHMHLNFVPVASGYKRGL
                     EKQVSFDRAITQQDPKLDKTRPFDDWREKEVKILEEMLKERGIERKLVGSNNYKDVNEY
                     KEKKDLEKEILSLENQLFEKKNELLEMNERLPGEIKIKAEREKKTEVVNKGFLKKEKVE
                     KPTGNWIVETKELKRVQGIINAGYSVKKDYDRLQRMDLVKENKELCDKVDSLAEGYVKA
                     INENTDLENENRELRKEISSLKAHIRDLKENVKVLYHNTKKLLGRQFEGFRGLIKNELD
                     MKGIDNQFEREHKKEMKTKQRGYDMER"
                     /name="plasmid recombination protein"
     gene            complement(1978..2520)
                     /locus_tag="HS527_RS00010"
                     /old_locus_tag="pAP3-9_02"
     CDS             complement(1978..2520)
                     /locus_tag="HS527_RS00010"
                     /old_locus_tag="pAP3-9_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360274.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000360274.1"
                     /translation="MDKNSQEKITIELNADEFKKLQKLLELDEKNEQDQQKKESEKKNL
                     NFIQLYRDNMPELRWLMANHNFASSLLFFIIEHMDNKNALACSYSTFEDYFGKSKMTIY
                     RGIKVLEENGFLSVLKMGTSNVYLVNEDLAWTDSNDKKKFTKYDGKILVSKKENKDYIY
                     RKQFDRFKALRKRENLK"
                     /name="replication/maintenance protein RepL"
     gene            complement(2705..2929)
                     /locus_tag="HS527_RS00015"
     CDS             complement(2705..2929)
                     /locus_tag="HS527_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017562246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032604260.1"
                     /translation="MVRLVQLLVQVGQLNLVFGLFGLAKAKNQPNFGMQWKKGGDFLSG
                     FLKKYYLPIAWRKTLLGDIERERKRSLDI"
                     /name="hypothetical protein"
     gene            complement(3150..3740)
                     /locus_tag="HS527_RS00020"
                     /old_locus_tag="pAP3-9_03"
     CDS             complement(3150..3740)
                     /locus_tag="HS527_RS00020"
                     /old_locus_tag="pAP3-9_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="WP_001024262.1"
                     /translation="MNILWDFDGTLFNTYPAYTKILSLVLGEEIDKQEIYKKLKISYSH
                     ALDYYNVSSDQKEKIKSLKKELSPKDMKPFDGVEEILKFADKNVIMTHKHRDGVLAILK
                     YYGWDKYFVDMVTIDNGFPRKPNPSAYTYLHQKHKIDLAIGDRELDLLPAKELGIITCM
                     FQGQSDVADYYLNDYSEFFNKGIPLSKQLYLQQ"
                     /name="HAD family hydrolase"
     Typing          complement(1979..2520)
                     /name="rep_cluster_1931"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          728..1938
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctattcccac aaggaatccg cctttgtctt tttctatgat tctatgcatt tttttataca
       61 ttcctattat ggcgggcttt tcggcctccc catagttccc tatgtcttcc gattttttgc
      121 ataaaatctt gcatactctt agcgtggttt ttttcaaaat gctggtggta ctccttaata
      181 acaagttcat agaatgttga ttttataaat caaaagagag gggaacctat gtagtgctac
      241 ttgagtagaa aattaggttt ttaattaggt agaggtagct gcatagcgtg tgtcaacggt
      301 accggaccga acccgtaagg gggcgggagg ataaggagtt gacacactca ctatgcaacc
      361 ctcttgtggt tgtcaaaagg tacccttttg actctgccaa ccggcgaggg agcccctcaa
      421 ggattgaggg gttggggagt tggattaagg gggtctgggg aagagttccc cagcaggttt
      481 ggacagagtc caaggttttg ttttttgtca tgcaaagcat ggcttggcga tagccaaaac
      541 agcctcgcag agccttttca ttgtgtacat ggagcgtagc ggaatgtcac aatacagaaa
      601 aggttacttt gccaaggtaa caaagctttt ggtatagtgg aaccaaggag ttgattcgtt
      661 atgagtatgt cagtttctct caagaaggca acgaacaaaa ccaacattag acacaataac
      721 aggacaatga gcgagaagga aaaagagcaa aattcgcaca tagattattc aaggtctgac
      781 gaaaataaat atcttgttca gaaagattta aagaaattat atcgagaaga atttggagaa
      841 gtgttggaga actataacgc caaacaaaaa cgtaacgacc gaaaaattga tgactattat
      901 aaacacattc agtccagtaa aaaaacatca cttcagcaag aaatgattat tcaagttggc
      961 gataaagatg atttttctag taaacaagat tttgaaaagg ctaacgaaat tttagaagaa
     1021 tggttattgg gatttgaaaa acgaaatcca aatttaaaaa tttataacgc agtcattcat
     1081 aatgatgaag cgagcccaca catgcatttg aattttgttc cagtagcaag cgggtataaa
     1141 cgtggacttg aaaagcaagt atcatttgac cgagcaatta cgcaacaaga tccaaaatta
     1201 gataaaactc gtccttttga tgattggcga gaaaaagaag tgaagatatt agaagaaatg
     1261 ttaaaagagc gtggtatcga acggaaatta gttggttcaa ataattataa agatgtgaat
     1321 gaatacaaag aaaaaaagga tttagaaaag gaaattcttt ctttggaaaa ccagcttttt
     1381 gaaaagaaaa atgaattact ggaaatgaac gaacgattgc caggagaaat taagattaaa
     1441 gctgagagag aaaagaaaac agaggttgtt aataaaggtt ttttaaaaaa agaaaaagta
     1501 gaaaaaccaa cagggaattg gattgttgaa actaaagaat taaaaagagt gcaaggaata
     1561 attaacgctg gatattcagt gaaaaaagat tatgatcgtt tgcagcgtat ggatttagtt
     1621 aaagaaaaca aagaattatg tgacaaagtt gatagtttag ctgaaggtta tgttaaagct
     1681 attaacgaaa atacagattt ggaaaatgag aatagagaat tgcgtaagga aataagctcg
     1741 ttaaaagccc atataagaga tttaaaggaa aatgtaaagg ttttatacca taacacaaaa
     1801 aaactcttag gaaggcaatt tgagggcttt agaggtctta ttaagaacga gttggatatg
     1861 aaagggatag ataatcaatt tgagcgtgaa cataaaaaag aaatgaagac taaacaacga
     1921 gggtatgaca tggagcgatg aaaacataaa taaagcgagt agactttact acccgcttta
     1981 ttttaggttt tcccgttttc gcaaagcttt aaatctatca aattgtttac gatatatata
     2041 gtctttattt tcttttttgc tgactaagat tttcccgtcg tattttgtaa attttttctt
     2101 gtcgtttgaa tcagtccacg ctaaatcttc atttactaaa taaacatttg atgtacccat
     2161 ttttagtaca ctcaaaaatc cgttctcttc aagtactttg attcctctat aaatagtcat
     2221 ttttgatttt ccaaagtaat cttcaaaagt tgaatatgag catgctaatg cattcttgtt
     2281 atccatgtgt tcgattatga aaaataaaag agaactagcg aaattgtggt ttgccattaa
     2341 ccatcttagt tctggcatgt tatctcgata aagttgaatg aaattaagat tctttttttc
     2401 actttctttt ttctgttgat cttgttcatt tttttcatct agttctaata atttttgaag
     2461 ttttttaaat tcatccgcgt ttaattctat agtgattttt tcttgggagt tcttgtccat
     2521 ttttccaccc cttttttgca agaaggtatc acactatgat acctatatgt ataatctttg
     2581 ttatatccta taacatgacc tgatatatgt aaagacaaat ttgttatatt cttctacaaa
     2641 taggtatcac aagctatgtt acctataggt atcacactat gatacctatt aaactctgaa
     2701 acccttatat atcaaggctt ctcttacgtt ccctttctat atctcctaat agggtttttc
     2761 tccacgcaat gggcaggtag tattttttga gaaacccact caaaaaatct ccaccttttt
     2821 tccattgcat tccgaaattc ggctggtttt tagctttcgc caagccgaac agaccaaaaa
     2881 cgaggttaag ttgcccgact tggaccagca attgtaccag ccgaaccacc cagttttatc
     2941 gatctaagca ataagcttac tctatattca gttacgttaa gttcttaggg gctagcccct
     3001 cgcttcgctt caccccagcc ttcaccactc cacccaaaaa caagacgatc gtttaaaata
     3061 aaaaataaaa taagctgtcc atatggacag cttatttaca taaaattcga aagcggaagt
     3121 caataaatct atcatatatc ttcagcgatt tattgctgaa gatacaactg ttttgataat
     3181 ggaataccct tattgaaaaa ttctgagtaa tcgtttaaat aataatctgc tacatcactt
     3241 tgaccttgaa acatgcatgt tatgattcct aattcttttg caggtagcaa atctaattct
     3301 ctatctccaa tagctagatc aattttgtgt ttttgatgca agtaagtata tgctgatggg
     3361 ttgggtttgc gaggaaaacc attatcaatc gtaaccatat ctacaaagta tttgtcccat
     3421 ccataatatt ttaaaattgc taaaactcca tctctgtgtt tatgtgtcat gattacattc
     3481 ttatctgcaa atttcaaaat ttcttccacg ccatcaaatg gtttcatgtc ttttggagac
     3541 aattcctttt ttaagctttt aattttctct ttctggtcac tagaaacatt atagtagtct
     3601 agtgcatgtg agtatgaaat ctttaatttt ttataaatct cctgtttatc tatctcttct
     3661 ccaagtacta gagagagtat tttggtatag gcaggatatg tattaaaaag tgttccgtca
     3721 aagtcccata aaatattcaa ttaaatcacc ctttctattc cattttatca caagaatgtg
     3781 gtagccatac tcttgttttc tttgtatact ccatacacta tcagttaaca taacgtctca
     3841 ttatcggtag tgaagacaaa ggcggatcc
//
