LOCUS       NC_010904               4715 bp    DNA     circular CON 02-JUL-2023
DEFINITION  Escherichia coli plasmid pColG, complete sequence.
ACCESSION   NC_010904
VERSION     NC_010904.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224531
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Avgustin,J.A. and Grabnar,M.
  TITLE     Sequence analysis of the plasmid pColG from the Escherichia coli
            strain CA46
  JOURNAL   Plasmid 57 (1), 89-93 (2007)
   PUBMED   16870252
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Ambrozic Avgustin,J. and Grabnar,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2005) Department of Biology, Biotechnical Faculty,
            University of Ljubljana, Vecna pot 111, Ljubljana 1000, Slovenia
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 07/02/2023 04:13:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.5
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            DQ286390.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CA46"
                     /db_xref="taxon:562"
                     /plasmid="pColG"
     gene            complement(99..752)
                     /locus_tag="HS389_RS00005"
                     /old_locus_tag="pColG_p1"
     CDS             complement(99..752)
                     /locus_tag="HS389_RS00005"
                     /old_locus_tag="pColG_p1"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249761.1"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_000560060.1"
                     /translation="MIAFSFSDIGNRCENLDLLSTINCQNDVFGMVIMDGYSCSEWNMK
                     ILSGRLDKCVFSGDLNKDIIEPCKSVPVKAAVCFLFKNNTHLHIYNAGDCRVYNKSGVL
                     LTEDNSVAWKRLKENGIDNKKIPHLVCYCPDRNYLTSFVDFCSDEIIFNHSVFDLNDEN
                     KEVILCTDGFWEHFNDGTVIDLMKDNPESNLAKFTKNIPGNAENHTVCYVRFPS"
                     /name="PP2C family serine/threonine-protein phosphatase"
     gene            complement(749..1615)
                     /locus_tag="HS389_RS00010"
                     /old_locus_tag="pColG_p2"
     CDS             complement(749..1615)
                     /locus_tag="HS389_RS00010"
                     /old_locus_tag="pColG_p2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000409995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_000409995.1"
                     /translation="MEKEINNWIEESGGKYRLMRQAVHIILKSISIEESLNEMMVMKGG
                     TLLGVKHGSDRYTKDIDFSTEKKLSEIDLGDFFNRFNDSMDVAEAELGYGIKCKVQSHK
                     IQPNAQGTFPTVQINIAFCDRSNASAMKRLESNQSPSVINIDFSFNEKTYDFDFLSLDG
                     DDDNDSVKTYSLTDLMAEKYRSVLQQKVRERNREQDIYDINYLLGKYEFSRQDKYKILE
                     SLLKKSEGKQLDNLLNVKGIRDVEIIERSSQRYQDLSSTVKSLPLFEDAYEKVACFYES
                     LPWDIFK"
                     /name="nucleotidyl transferase AbiEii/AbiGii toxin family
                     protein"
     gene            complement(1660..2556)
                     /locus_tag="HS389_RS00015"
                     /old_locus_tag="pColG_p3"
     CDS             complement(1660..2556)
                     /locus_tag="HS389_RS00015"
                     /old_locus_tag="pColG_p3"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein epg3"
                     /protein_id="WP_012477790.1"
                     /translation="MYLIEEALTSWLLNEYKKPVITKEEISLQIYLFYLKKEYNGERIY
                     QIRKNKAGPNEFEKNILKLLKSGVLSRVESNDKHHAYPHLIGGFYEVFFVKGNDGSPEN
                     DIVCSTYPYGYLSYLSAMDWHNITDKIPKIIHFTTCRSEQWKNKFYHDFQKDNPDIDEA
                     KQFMPRFPRNSEVNGIKIIVTQETNYVEPMKARNSPVRVSSIGKTFMDMTRRPDYCGGE
                     EHVLESFIEHGKKYAPLIIKEVDKLGRHIDKVRVGFILDRIIGVNNKKISEWNQEAGKY
                     RGSSESAISKESILTLL"
                     /name="protein epg3"
     gene            3632..3826
                     /locus_tag="HS389_RS00020"
     CDS             3632..3826
                     /locus_tag="HS389_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041633.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQVRFETESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3118..3373)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="89.0"
                     /evalue="1.21e-116"
     Typing          complement(3498..3596)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(3900..3982)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.79e-36"
     Typing          3995..4551
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.652"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agagaaaacc gccccgcaaa ggcggttttt ttcttttaca gaaaagagat tatctatatg
       61 acacgaagat ctcaatagct tcttcaaaaa acacgaaatc aagaagggaa tctaacgtag
      121 caaaccgtat gattctctgc gttacccggg atatttttag taaatttagc caagttagat
      181 tctgggttat ctttcatgag gtcaataaca gtaccatcat taaaatgctc ccaaaaccca
      241 tcagtacata aaataacctc tttgttttca tcatttaaat caaaaactga atgattgaaa
      301 attatctcat cagagcagaa atcaacaaat gatgttagat aatttctgtc tgggcaataa
      361 caaacgaggt gaggtatttt tttattatcg attccatttt cctttaatct tttccaggca
      421 accgagttat cctctgttag aaggactcca cttttgttat atacacggca atctcctgcg
      481 ttatatatat ggaggtgcgt attattttta aaaaggaaac atactgcagc cttaacagga
      541 actgacttgc atggttcaat aatatcttta ttcagatcac cagaaaacac gcacttatct
      601 aacctaccag agagaatttt catattccac tcagagcagg aatacccatc cataataacc
      661 attccaaaca catcattttg acaatttatc gtagataata agtctaagtt ttcacatctg
      721 ttaccaatat cactaaaact gaacgctatc atttgaatat atcccacgga agactttcat
      781 agaaacaggc aactttttca taagcatctt caaaaagagg taatgattta acggtggatg
      841 ataaatcctg atatcgctga ctggaacgct cgataatttc tacatccctt atacctttaa
      901 cgtttaagag attatccaat tgtttacctt cactcttttt taaaagagac tccagtattt
      961 tatacttatc ttgccgagaa aactcatatt tacctaaaag ataattgata tcatatatat
     1021 cttgctctcg atttctctct ctaacttttt gttgcaaaac ggatcgatat ttttcagcca
     1081 tcaaatcagt taagctgtaa gtctttacgc tatcattgtc atcatcacca tctaatgata
     1141 aaaagtcaaa atcatacgtt ttttcgttaa agctaaaatc gatatttatc actgaaggtg
     1201 attgattaga ttcaagcctt ttcattgcag acgcattaga acgatcgcaa aaggctatat
     1261 ttatctgtac agttggaaat gttccttgtg cattaggttg aattttatga gattgaactt
     1321 tacatttaat accatatccc aattcagcct cagcaacatc cattgagtca ttaaacctat
     1381 tgaaaaaatc ccccaagtca atttcagaaa gttttttttc ggtagaaaaa tcaatatcct
     1441 ttgtgtatct atcactacca tgcttaacac caagaagagt ccctcccttc ataaccatca
     1501 tttcattcaa actctcctct atagaaatag atttaagtat tatatgtaca gcctgccgca
     1561 tcaacctgta cttcccacca gactcttcga tccaattatt aatttctttt tccataagac
     1621 ttaaccatat caacattaat agaaataaac cattcttcat caaagtaagg tgagaatgga
     1681 ctctttggag atagcacttt ccgaactacc tctgtattta cccgcctctt ggttccattc
     1741 gcttattttt ttattattaa ccccaataat tctatccaaa atgaatccaa cccttacctt
     1801 gtcaatatga cggccaagct tatcgacttc ctttataatc agaggtgcat attttttacc
     1861 gtgctcaata aatgattcca atacatgctc ctcaccacca cagtaatcag gccgcctggt
     1921 catatccata aaggtcttac cgatagatga taccctgaca ggggaattac gagccttcat
     1981 tggctcaaca taatttgttt cttgtgtaac gataattttt attccattga cctcagaatt
     2041 tcttggaaat ctaggcatga attgtttggc ttcgtctata tcaggattat ccttttgaaa
     2101 atcatgatag aacttattct tccactgttc ggaacgacat gtcgtaaagt ggattatctt
     2161 gggtatttta tctgtgatgt tatgccaatc catagcggat aaataggata agtatccata
     2221 tggatacgta gagcacacta tatcattttc cggagaacca tcgttacctt taacaaaaaa
     2281 gacctcatag aaaccgccaa ttaaatgggg gtaagcatga tgcttatcgt tactttcaac
     2341 tcttgataaa acccctgact ttaataattt taatatattt ttttcaaatt cattaggtcc
     2401 tgctttgttc tttctaattt gatatatcct ctcaccatta tattcttttt ttaaataaaa
     2461 aaggtaaatc tgcaaagaga tttcttcttt ggttatgact ggttttttat attcattcaa
     2521 caaccaagac gttagcgctt cttcgataag atacatattc aaccttttcg gtttagtctc
     2581 taatagaata tacgtcctaa ggacgtatat tctattagag attgattgtc aagaaaaaaa
     2641 taataacaaa caagcatttg ccatttttat atttttgata tcctataaaa taaatcctta
     2701 ggatttattt tataggataa taagtaggaa gtcaagaaaa agtttactta tatcaccgtt
     2761 ttcataacac ctcatgcgat caattcagcg gcggttatag ttcaaatcca tgattaggac
     2821 atctgttgct cataaacgat gagcgtaaaa gatcattttt cagcgtcatt taccataacg
     2881 tcgcattacg cgcaccgcta aaacgcgctg aacgcgattc tgccgcgaac accgcaaaaa
     2941 gagatgtttt accaagcgaa aaccggaaaa atgcgtcagg atcgctttca gctcactgca
     3001 tagctatgca tgaaagtgaa tggcgatcgg tttgggcgca tacggtgccc atgagacaca
     3061 ttttcggaca ttttcagttc gtgaatccct gactcattgc tacatatctg aacagacacc
     3121 cctagcagcg cccccagggg cgtcctgtaa aaaaacacac cgcgccgcta gcagcacccc
     3181 taatataaaa tgatgttttt tatgaaaata gtcagtacca cccctaaaaa gcggtgttgg
     3241 cgcagctaca acaccgcgcc gacaccgctt ttttaaatat cacaaagaga gcaagaagaa
     3301 ctaatttgtc ataactctct atttataaag aaaaatcagc aaaaaattgt ttttgcgtgg
     3361 ggtgtgggca tttcaggcat gagaacagac tcgcgttttt ccggagtatc agcggaatta
     3421 ttgattaaga aacactcgac gcagagcgaa attttatgca acagcggctg cactgcacag
     3481 ggattattta tcagttgata tgctgtgcca tgatacgtcc taaaaaaaca ggacaggatg
     3541 tgcaattgta ataccgtcgc ccgcaacgtc ttacaattac cacctggtca gggtccaccc
     3601 gacacccggt actgacggag aacaacggaa ggtgaacaaa cagcaacaaa ctgcgctgaa
     3661 tatggcgcga tttatcaaaa gccagagcct gacactgctt gaaaaactgg atgctctgga
     3721 tgccgacgaa caggcggcca tgtgcgaacg actgcacgaa ctcgcggaag aactccagaa
     3781 cagcatccag gttcgctttg agaccgaaag tgaaacagga acataacgaa gctgacggag
     3841 gcgctcacgg cttgtccgcg agcgttttcg gcagcagaca agcccgccag ggcgcgtcag
     3901 cgggtgtcgg ggcgaagccc tgacccagtc acgtagcgat agcggagtgt atactggctt
     3961 aactatgcgg catcagtgca gattgtatga aaagtgcccc atgccgggtg tgaaattccg
     4021 cacagatgcg taaggagaaa atgcgcgtcc aggcgctttt ccgcttcctc gctcactgac
     4081 tcgctacgct cggtcgttcg gctgcggcga gcggtactga cttacacaaa aacggcagca
     4141 cggttatcca cagaatcagg ggataaggcc ggaaagaaca tgtgagcaaa agaccaagaa
     4201 caggaagaag gccgcgccgc aggcgttttt ccataggctc cgcccccctg acgagcatca
     4261 cagaaatcga cgctcaagtc agaggtggcg aaacccgaca ggacttaaag ataccagacg
     4321 tttccccctg gtggctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4381 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggatc
     4441 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4501 ccgactgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgcct
     4561 taacgccact ggcagcagcc actggtaact gatttagagg agttagtctt gaagtcatgc
     4621 gccagttaag gctaaactga aagaacaggt tttggtgact gcgctcctcc aagccagtta
     4681 ccttggttct tcgagccgca aactctttga accta
//
