LOCUS       NC_010898               4995 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pIGRW12, complete sequence.
ACCESSION   NC_010898
VERSION     NC_010898.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225719
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Wolinowska,R., Strzezek,K., Lakomy,A.A. and Plucienniczak,A.
  TITLE     pIGRW12, a cryptic plasmid isolated from a clinical strain of
            Escherichia coli
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Wolinowska,R., Strzezek,K., Lakomy,A.A. and Plucienniczak,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2006) Bioengineering, Institute of Biotechnology
            and Antibiotics, Staroscinska 5, Warsaw PL-02-516, Poland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009951385.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 02:53:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            EF088686.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /plasmid="pIGRW12"
     gene            210..533
                     /locus_tag="HS506_RS00005"
                     /old_locus_tag="pIGRW12_p1"
     CDS             210..533
                     /locus_tag="HS506_RS00005"
                     /old_locus_tag="pIGRW12_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_012477689.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            742..>1176
                     /locus_tag="HS506_RS00040"
                     /pseudo=""
     CDS             742..>1176
                     /locus_tag="HS506_RS00040"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1211..1702
                     /locus_tag="HS506_RS00015"
                     /old_locus_tag="pIGRW12_p3"
     CDS             1211..1702
                     /locus_tag="HS506_RS00015"
                     /old_locus_tag="pIGRW12_p3"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000096688.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            1709..1924
                     /locus_tag="HS506_RS00020"
                     /old_locus_tag="pIGRW12_p4"
     CDS             1709..1924
                     /locus_tag="HS506_RS00020"
                     /old_locus_tag="pIGRW12_p4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2411..2638)
                     /locus_tag="HS506_RS00025"
     CDS             complement(2411..2638)
                     /locus_tag="HS506_RS00025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053504437.1"
                     /translation="MGYHYKELTVIELTAEEFELHKEGYSLELLTNMRNEEHTKYWEVC
                     PSCEGKMDPEENQGHEGYCATCWLDNPDWW"
                     /name="hypothetical protein"
     gene            3413..3751
                     /locus_tag="HS506_RS00030"
     CDS             3413..3751
                     /locus_tag="HS506_RS00030"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_176450233.1"
                     /translation="MFMSNDENEYTFKPVNAQAIKALKSLPEWVQAAFVHALDSVFYGV
                     DPGMSIAHLTVIGPGVVELRINGRPAYRCVYRSKAPGQVDLLHAFVKTCQGPDKKNMAT
                     TASRYKLL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3767..3982)
                     /locus_tag="HS506_RS00035"
                     /old_locus_tag="pIGRW12_p5"
     CDS             complement(3767..3982)
                     /locus_tag="HS506_RS00035"
                     /old_locus_tag="pIGRW12_p5"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_242596674.1"
                     /translation="MIDNKWSQSEAAEKLGISQPRVSNMLNGKLDKFSVDTLLEMVFKM
                     GYKLDMDFTPLNTESPLTMVVKKAMV"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4425..4556)
                     /locus_tag="HS506_RS00045"
     CDS             complement(4425..4556)
                     /locus_tag="HS506_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_058645281.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPEKHDLTPLAAATGKGCTRDN"
                     /name="hypothetical protein"
     Typing          2..97
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.938"
                     /coverage="100.0"
                     /evalue="1.27e-39"
     Typing          complement(4426..4952)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          524..2016
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.8"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaagcacttc actgacatcc gcattcgtgc caacatagta agccagtata cactccgcta
       61 gcgctacgtg actggttcag ggctgcgccc cgaaacccgc caaaagcctg acgcgcctgc
      121 ggcttgtcca accccgcgcc gaccgggaaa ggctttcccg cccgtcccgg cgttatcagg
      181 aggccggttt gtcagagaaa cggagcaaga tgctcaccat gtgggtgacg gaggacgagc
      241 accggcggct gctcgagcgc tgtgacggca ggcagctggc ggcgtggatg cggcagatct
      301 gcctggacga aaagccggca cgttccggaa agctcccctc gatctcgccg gcgctgctgc
      361 gccagcttgc cggcatgggc aataacctga accagatagc ccgccgtgtt aacgccggcg
      421 gcggcaccgg ccatgacagg gtacagattg tggcggcgct gatggccatc gatgccggcc
      481 ttgagcggct gcggcacgcc gttcttgaga aaggaacgga cgatgatcgt taaatttcat
      541 ccgcgcggcc gcggcggtgg cgccgggccg gtggactatc tgctgggtaa agatcggcag
      601 cgtgaaggcg ccaccgtcct gcaggggaag ccggaagaag tccgggagct gattgacgcg
      661 tcgccttacg ttaagaaata cacctccggc gttctgtcgt ttgcggaggc cgatctgcct
      721 cccggccagc gggaaaaact gatggccagc ttcgagcggg ttctgatgcc cggactcgat
      781 aaagatcagt acagcattct gtgggtggag cacgcggaca aggggcgtct ggagctgaac
      841 tttctgatcc ccaataccga gctgctgacc ggcaggcggc tccagcctta ctacgaccgc
      901 gccgaccggc cgcgcatcga tgcctggcag actatcgtga acggccgact ggggctgcac
      961 gacccgaacg cgccggaaaa ccgccgggcg ctggtcacgc cgtccgggct gccgaaaacg
     1021 aagcaggagg ccgcagaagc cattacccgg ggactgctga cccttgcttc gtccggggag
     1081 ctgaaaagcc gtcaggatgt cactgacact cttgaaggtg caggttttga ggtggtgcgc
     1141 accacgaaaa gcagtatcag cattgccgac ccggacgggg ggcgaaacat ccgactgaag
     1201 ggagctatct atgagcagtc ttttaacgct ggcgaaggac ttagagcagc aatcgaaagc
     1261 gcagcagcag agtaccggcg agatgctgaa agccgcattc agcgagcacg agcagtctgt
     1321 cagagcggaa ctgagcgcaa gcgcgaggag aatcagcgac gccatcctcg cccacgagca
     1381 gagtatgagc gaggcgatgg agaaaaaccg gcggagcgta ctgcggactg ccgggcggac
     1441 atggctcacc atcctgatgg tgtcagcgct gctgatcggc acgagcggga gcattctgtg
     1501 gtggcagggg cagcagataa ccgacaatta cacgcatctt cgtcagcagg aagataccct
     1561 ggcgaaaatg accgcccgga cgtggggagt gcgctatcag gagagcagcg acggccggag
     1621 gtttcttatc ctgccgccgg ggatgcaaac cgaagccatc ccctacgacg ggacgacatg
     1681 gatccgcctg aaacaggagt agcggaaaat gacagagctg gaaaagcagt tgctgagcgc
     1741 attagagcag ctacagcagg actactcgca aaggctggac gaatgggaga gcgccttcgc
     1801 ggaatggcgg agcatgtgtg ggcttatgca acgggagaac gcggtgctga acgagcgcgt
     1861 gtcggacttg agcacgcagg tgctgagttt aagcgagcag ctgcgccgct tgtccgggaa
     1921 ctgaatgcca ccgagttccg gcagagggag aatgagtatc agaaaacact ggagctggag
     1981 cgccagccgc agaaaaccta ccgcgggccg acactttaaa ccatccaggc agctgacaac
     2041 ccgcacgaag aggggctctt atttctcccg ctaaataggt tcgtgatcaa aaaggtcacg
     2101 acttattcag gagcgggaaa tgaagctgtt aaatcttatt gaatcattgc ttaactgcct
     2161 gtatctggcg gcggagctgt gggactggct ggagaagcat ttgtcggttt ttaaggcatt
     2221 ggcgcggatt ttagtgtgac tttaacatcc atgcatctgg ctgcatgact atgcaccagt
     2281 gtaatattca gcggctgcgt ttctggtgtt ttttcgttat gtctgtcgcc actttctcgc
     2341 gtctggcacc ggaaacgcag ccaaccccgt ttcagcggtg cgggcaacgg atgttatggt
     2401 aaattattaa ctaccaccag tcagggttat cgagccagca tgtggcacag taaccttcat
     2461 gcccttgatt ttcttctgga tccatttttc cttcgcagga agggcaaact tcccaatact
     2521 tggtgtgttc ttcatttctc atgttagtta aaagctctaa agagtatccc tctttatgaa
     2581 gctcaaactc ttccgcagtt aattcgatga cggttaattc cttgtaatgg taacccattc
     2641 gcttgcctcg ctttatgcag ctcaatacta agatactgaa aagcattaaa tgtcagggtt
     2701 ttcagtatct tacaatgata atcctatccg gtttatacaa gggatattac cgctttacta
     2761 agcagaagat gaattactct gtgttatctg gcgtgcggaa aatttgcagc caatggcggc
     2821 ctggaacgct ggttggaggg gcgtactcat tatcgcggat tgtgcagtag cggccatatt
     2881 ttcgggttac cgtatgattc gttgtgttga tatcacattc ctggaatgaa ccaccattac
     2941 gacctacttt gctgtgagga ccaacataaa tagccattct aagctccttc gtcagatagt
     3001 tgattaccag tatctaagta gattttatta atttctatat ttatcaatat catagtgtgc
     3061 tttgttggct ttccacattg cgtcgataaa atcctcatat cttgatgtaa ggaaaaccaa
     3121 cacatgtaac tgttaggtct tctgcgccta agtcaaattg attttttttt attcaaacag
     3181 acgtttgaag cactaataag aattgatgac atataagaat ttatatacta tggcatcgtt
     3241 ggcgagattg ccaatgaatt gttggttcaa cgagcaataa ccatgcgcgc actttcctaa
     3301 atatagggcg gtgtggtttt tttatgctca taaatcaatc aatcaatcgg agtttatatg
     3361 cgaaacgttt taacaatagt taagttgcta aaaatcaata acctagatga ggatgtttat
     3421 gtcaaacgac gaaaatgaat acacgttcaa acctgttaat gcccaggcta tcaaggcatt
     3481 aaaatcactc ccagaatggg ttcaggctgc attcgtgcac gcattggatt cggtgttcta
     3541 cggggtggat ccggggatgt ccatcgcaca cttgacggtc atcggccccg gcgttgttga
     3601 gttgagaatc aatggtcgcc cagcttatcg ctgtgtatac cgcagtaagg cacccggcca
     3661 ggtggactta ctgcatgcgt ttgtgaaaac gtgccaagga ccagataaga aaaacatggc
     3721 aaccacagct tcgcgctaca agttgctcta atcaaattgg cgatggttac accatcgcct
     3781 tttttacaac catagtcagg ggcgactctg tgtttaatgg agtgaagtcc atatccagct
     3841 tgtaccccat cttgaaaacc atttccaaca gcgtatcgac ggagaatttg tccagttttc
     3901 cgttcagcat attgcttact cttggttgac tgatacctaa cttttctgcc gcttctgatt
     3961 gcgaccattt gttatcaatc attagttttc taattatgat aatgagtttt gacttgatcg
     4021 accatgtatt gaactttact gggtctgtac tgatcagctc aaatgaattt ttcatagaac
     4081 agctccactt cggattacct gtatcgcaat gataaaagtt ccggcaagga actccttata
     4141 tatatatacg ggcggggcat tatacaagta atgatattgc tttcaagagg aaaaagtgta
     4201 aagacatttt gatcttttaa gtaaagctta ttgtgttgac gtatcaatcc agcaaagagt
     4261 agtctttgcc cataaacgaa aaaaccaccc ggcaaggtgg tttttcgaag gttcattaaa
     4321 ccggcaagtt tatgaaccgt ggtaacaggg tgtacaagac cgctgccacc taattcgtcc
     4381 ttccagttta gccgtagttg gactgatact tcaagaactc tgcatcagtt atctcttgta
     4441 caccctttac cagtggctgc cgccagtggc gttaagtcgt gcttttccgg gttggactca
     4501 agtcgatagt taccggaaca ggcgcagcag tcggactgaa cggggggttc gtgcatacag
     4561 tccagcttgg agcgaactgc cttcccggaa ccgagtgtca ggcgtggaat gagataaacg
     4621 cggccataac agcggagtga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag
     4681 ggagccgcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg
     4741 atttgagcgt cagattttgt gatgcttgtc aggggggcgg agcctatgga aaaacggctt
     4801 cgccgcggcc ttattgcttc cctgctaagt ttcttcctgg catcttccag gacatttccg
     4861 ccccgccagt aagccatttc cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4921 agcgaggaag cggaatatat cctgtatcgc atattctgct gacgcgccgg tgccgtattt
     4981 tttctccttc cacat
//
