LOCUS       NC_010896               8271 bp    DNA     circular CON 30-JAN-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Typhimurium plasmid
            pAnkS, complete sequence.
ACCESSION   NC_010896
VERSION     NC_010896.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225676
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Typhimurium (Salmonella
            typhimurium)
  ORGANISM  Salmonella enterica subsp. enterica serovar Typhimurium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Sahin,F., Karasartova,D., Gerceker,D., Aysev,D.A. and Erdem,B.
  TITLE     Sequence analysis and characterization of plasmid pAnkS from
            Salmonella typhimurium
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Sahin,F., Karasartova,D., Gerceker,D., Aysev,D.A. and Erdem,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2006) Microbiology, Universty of Ankara, Sihhiye,
            Ankara 06100, Turkey
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 05:05:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9
            CDSs (total)                      :: 9
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            DQ916413.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Salmonella enterica subsp. enterica serovar
                     Typhimurium"
                     /mol_type="genomic DNA"
                     /serovar="Typhimurium"
                     /sub_species="enterica"
                     /db_xref="taxon:90371"
                     /plasmid="pAnkS"
                     /note="clinical isolate"
     gene            complement(join(8256..8271,1..134))
                     /locus_tag="HS445_RS00005"
     CDS             complement(join(8256..8271,1..134))
                     /locus_tag="HS445_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001677812.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EEGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            417..737
                     /gene="mobA"
                     /locus_tag="HS445_RS00010"
                     /old_locus_tag="pAnkS_p1"
     CDS             417..737
                     /gene="mobA"
                     /locus_tag="HS445_RS00010"
                     /old_locus_tag="pAnkS_p1"
                     /function="plasmid mobilisation"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_010891087.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAECGKTVSGFLRAA
                     ALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRLM
                     AD"
                     /name="mobA"
     gene            complement(972..1352)
                     /gene="lsoB"
                     /locus_tag="HS445_RS00015"
     CDS             complement(972..1352)
                     /gene="lsoB"
                     /locus_tag="HS445_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="WP_000710826.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(1349..2389)
                     /gene="lsoA"
                     /locus_tag="HS445_RS00020"
                     /pseudo=""
     CDS             complement(1349..2389)
                     /gene="lsoA"
                     /locus_tag="HS445_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /name="lsoA"
     gene            complement(2654..2767)
                     /locus_tag="HS445_RS00045"
     CDS             complement(2654..2767)
                     /locus_tag="HS445_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001459398.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
                     /name="hypothetical protein"
     gene            2973..3128
                     /locus_tag="HS445_RS00025"
     CDS             2973..3128
                     /locus_tag="HS445_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001490772.1"
                     /translation="MSVDFCDARQGGGAYGKTPAALASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(3348..6353)
                     /locus_tag="HS445_RS00030"
                     /old_locus_tag="pAnkS_p2"
     CDS             complement(3348..6353)
                     /locus_tag="HS445_RS00030"
                     /old_locus_tag="pAnkS_p2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            6517..7074
                     /locus_tag="HS445_RS00035"
                     /old_locus_tag="pAnkS_p3"
     CDS             6517..7074
                     /locus_tag="HS445_RS00035"
                     /old_locus_tag="pAnkS_p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            7257..8117
                     /locus_tag="HS445_RS00040"
                     /old_locus_tag="pAnkS_p4"
     CDS             7257..8117
                     /locus_tag="HS445_RS00040"
                     /old_locus_tag="pAnkS_p4"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     ARG             7258..8114
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          170..408
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.72e-122"
     Typing          complement(2696..3227)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.434"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          418..734
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="3.63e-60"
ORIGIN
        1 gcgagttcgt gcagcttctc acacatggca gcctgttcgt cagcatcgag tgcgtccagc
       61 ttttcgagca gcgtcaggct ctggcttttt atgaatcctg ccatgttgag tacagcttgc
      121 tgctgcttgt tcatcttttc gttttctccg ttctgtctgt catctgcgtt gtgtgattat
      181 atcgcgtacc acttttcgac tgttttaccg ccggtattct cgcgtctgac gctttgacgg
      241 gtatttctgc ctgacaatac tgtcactgcc aaaaaactgc cgtgcctttg tcggtaattc
      301 gagcttgctg acaggacagg atgtgcaatt gttataccgc gcatacatgc acgctattac
      361 aattaccctg gtccagttcg ccccgacacc ccatgtcaga tacggagcca tgttttatga
      421 caaaacgaag tggaagtaat acgcgcaggc gggctatcag tcgccctgtt cgtctgacgg
      481 cagaagaaga ccaggaaatc agaaaaaggg ctgaatgcgg caagaccgtt tctggttttt
      541 tacgggcggc agctctcggt aagaaagtta actcactgac tgatgaccgg gtgctgaaag
      601 aagttatgcg actgggggcg ttgcagaaaa aactctttat cgacggcaag cgtgtcgggg
      661 acagagagta tgcggaggtg ctgatcgcta ttacggagta tcaccgtgcc ctgttatcca
      721 ggcttatggc agattaactt cccggagaga aactgtcgaa aacagacggt atgaacgccg
      781 taagccccca aaccgatcgc cattcacttt catgcatagc tatgcagtga gctgaaagcg
      841 atcctgacgc atttttccgg tttaccccgg ggaaaacatc tctttttgcg gtgtctgcgt
      901 cagaatcgcg ttcagcgcgt tttggcggtg cgcgtaatga gacgttatgg taaatgtctt
      961 ctggcttgat attatattgg aatgcctttt ttcaaagcaa atgatgtggc tttggataga
     1021 aggtttacgt tgatcttatc aaagtttttt ttaaagaacg aagccgagag ctcagataaa
     1081 tcattatatt catcagtttt cgtaactttg tttaatgtgt aacttgaaaa cttctcgcca
     1141 ttaaatgacg tatagacgta acgatctttt tttccaccgt taggaattat taaatcaaaa
     1201 aaaacatcac ccttgctttt ctttttcttc aagtcggatt cgatttttga gaaaaattcg
     1261 ctcgggctat aaatatcagt agcatagaca ataaataaag ttttatcttt attttttatt
     1321 gcttctattt gatatttttt atcttttttc ataatttcaa cctacgtacg ccaccatcta
     1381 ttaattggca aacggtatcg atgattgcga ttgattctaa tttgttaatt gtcttcgtgt
     1441 cagctattcc tggtttcata tgaaacaaac catgcctgtt ctcatgccag taagtgtagc
     1501 attcacacaa aacttccgct atttcaccat ttatagtttc ttggtgtatt tctctgatta
     1561 tatatttggg tttgttttca gtgaagtatt cgccaaggtt ctttgatgat gatggattgc
     1621 aaacatcatt aagtatttga tatataaagc cttctatggc tcttaatgca gagaagcagt
     1681 atgttgagta atcttccatt tcgacatcta tttttttcat tattagcgag catgatagct
     1741 attttttgat atcttcatgg attttatcga tgctttttgg tagtttgcta tgcaactcgg
     1801 actcaatagt ttcttttttt atgtcaacat taaattcttt atttttttgt tcgacaatct
     1861 ctttcatgtt tagtattgag cacatgaaat cgttaatcaa actcgcgatt tgaaggtatt
     1921 ttccttggaa ttgaatagag ccgcgcttgt aaatttttgc cctgaccctg tcaccattgc
     1981 tggtggtcat aatatattgg tgtttacaat taggatcgtt attattatct tctgttattg
     2041 ttatcccctc ttcagaaaga aattcaaata gatttgccct gtcatcatca ctgaattttg
     2101 gaatggtgta ttcaaagttc tttgtgtctg aatacaaaca gttttctttt ataatcaagg
     2161 cgatttcatc aaagtaagtg ttattttgcc cagacgctct tccgatagtg gtatttccgc
     2221 ctgatggcat tagttttatt aagaagtcta ttcctttata tgtgccagat atgtgagttt
     2281 ctctttcgtt ttttacatta gaggaatagt ttgtgacgcc attctgcgtc agagcagact
     2341 caatcttgtc aatattgata tttagtgctt taaacgggtt ctgtgccatt gggtcaatcc
     2401 gttgtttttt ttgaatatgt acagatcttg tttttttgtc aacggaatag ctgttcgttg
     2461 acttgataga ccgattgatt catcatctca taaataaaga aaaaccaccg ctaccaacgg
     2521 tggttttctc aaggttcgct gagctaccaa ctctttgaac caaggtaact ggcttggagg
     2581 agcgcagtca ccaaaatctg ttctttcagt ttagccttaa caggtgcata acttcaagac
     2641 aaactcctct aaatcagtta ccaatggctg ctgccagtgg cgataagtcg tgtcttaccg
     2701 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2761 cgtgcacaca gcccagcttg gagcgaacga cctacaccgc actgagataa ccccacagcg
     2821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2941 ttatagtcct gtcgggtttc gccacctctg gcttgagcgt cgatttttgt gatgctcgtc
     3001 aggggggcgg agcctatgga aaaacgcctg cggcgttggc ttcttccggt gctttgcttt
     3061 ttgctcacat gttctttccg gctttatccc ctgattctgt ggataaccgt attaccgctt
     3121 ttgagtgagc tgacaccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg
     3181 aggaagcgga agagcgcctt atgtgacatt ttctccttac gctctgttgt gccgttcggc
     3241 atcctgtcct gagagttatc tctctgtgct attttctact tcaaagcgtg tctggatgct
     3301 gttctggagt tcttggggtc tgacgctcag tggaacgaaa actcacgtta agcaacgttt
     3361 tctgcctctg acgcctcttt taatggtctc agatgtcctt tggtcaccag ttctgccagc
     3421 gtgaaggaat aatggccgag catattgata tgtccgtggc aaagcgggga gaggcgtgcg
     3481 atatcttcat cattcagtgt ttcaccctgc gcccggagat gatccagggc tgcctgcata
     3541 taaatagtgt tccataacac gacggcgtta gtgaccagcc ccagtgtgcc cagttgatct
     3601 tcctgaccgt cggtatatcg ttttcttatc tcaccttttt gaccgtgaca gatggctctg
     3661 gcaacggcat ggcgactttc tccccgatta agctgggtca gaatgcgccg gcggtaatct
     3721 tcatcatcaa tataattaag cagatacagc gttttgttga tgcgccccac ttcaatgatt
     3781 gcctgagtca gtccggaagg acgttcactt ttcagcaatg aacggaccag cactgaaacc
     3841 tgtactttgc ccagcttcag ggagccagcg gtccggatca tttcgtccca ctgaaggact
     3901 atttttcggg gatctgattg ccctctggca atatcattca gcacgccata gtcggcatca
     3961 tggtccattc gccagaaaac cgaagcaccg gcatcagcca ggcgtggaga aaactggtat
     4021 cccagcagcc agaaaaggcc aaagacaagt tcgctggcac ctgctgtatc ggtcataatt
     4081 tcggttggat tcagcccggt ctcctgttcc agaagacctt ccagcacaaa gatagagtcc
     4141 ctcagcgtcc ccggtataac gatgccatga aagccggaat actgatcgga cacaaagttg
     4201 taccaggtga tccctctgtt attaccaaag tatttgcggt tcggtccggc attgattgtt
     4261 ctgactggcg taacaaagcg cattccatct gcagatgcca cttctcctcc accccatatc
     4321 tgtgccagtg gcagcgttgc ctgaaaatca accagtctgg cattagcgct ggtgatagtt
     4381 tcagcccgca gatagttcgc ttttgtccag ttcagccggt gtcgggtcag tgcaggaaca
     4441 tttgatctga tcagtggttc cagaccgata ttgcaggctt cagccatcag cacggcgctg
     4501 atgctgacgg gcagatcatc aactctggca ctggcttcac tagcatggaa aaactcatca
     4561 gcaaatccgg tatgggcgtt aatttcgagc agcaactccg ttaaatccac cggagggagt
     4621 agatcactga tcattttgct cagtcgtttc agactgtccg gctcatcaag actggcgagg
     4681 ggagaaattg tcaaccgggg cttcgggcca gaaacatcga gttcgacagc ctcattttcg
     4741 caaagacgtg cagcaacctg tctgtaacga ctatcaagct gatgacccag agattttatt
     4801 gcttcctgcg ggtctgtcgg gtgccccaaa gaacgataaa ccttaatccg gtttgcctgc
     4861 cagtcagcac cctgtagtaa tcttgcacga ggatctcccc accggttact gccggtaacg
     4921 tagacatccc tccgcctcag actatcctgc agtttactga gaaagcagag cgtgtatccc
     4981 ctgcgggtga tatgtttttc cttgttaatc accagccgtt tccatgaccg actgataatt
     5041 tccgttggtg cgtcgtcaaa aaactgccgc cgtgagctga actcccggct gaggtagtca
     5101 caggcattca gagtggtaac cccggcaggt gcggatgaaa atttaacggt attcagcaga
     5161 tggggcagga aacgacgaac gcgcccgtac tgctccacca tttcttcatg aaaattatcg
     5221 tctgagggcc gggcaatttc acggacaagc gtgatgattt cagccagctt ttgccttggg
     5281 atgtagctga acacctcagc acgaatcgat tcgtccggtg tttcttcttt cagcaggtac
     5341 gaacatgcgc tggcgagcgc caatgcagat ttatccagat ccttcagcga gcggagccgt
     5401 tttttctgcc caatctttct ggcgtcacgg atgataacgg ccagcatggc gtccagaacg
     5461 tccaatgcat catccagcgc cagcgtttcc catgcaagga caaaggcaac cagaaccgcc
     5521 atccttttct gcggtgacat cctggcaata ttgaacaccg aagtcatacc agcataacgt
     5581 gcgagatttt tcaggcgcac agccgggagt gtactcaggt tttcagcatg caggccaaaa
     5641 tcgttcagag ttttccagcg ttcaattgct tcattaaacg ccggaccact gatggtcaca
     5701 gggccctttt tcagtgattc cagtaaagac aggcggctgc aatcagttgg ccccagcagc
     5761 atctccagct gtgaacgctg ttcggctgac ggtatcagtg ccagtttgtt ccacaggcgc
     5821 aacgtcgcct tttcccttac ctctgaaatc aaccgggtca gcgtagtggc tccggggaga
     5881 ataatacgat gttgcataag ccaccctgtc gccagatcga aaagcaggcc aggacgttcg
     5941 ttgcttatcc agctccgggt atataaaaga cgggtaaggc gaaatgtcca gggccaggca
     6001 aattcacgat actgatagtg ctgacgtatc agcgctgcat gctcacggcg ggtattttcc
     6061 ctctgaccgt attctgcaag aacggtgata tcacgaatcc cgagctgtct ggcggtaaaa
     6121 tgccggacgc cggaaggaat atgattcata tcggtgagga aggtgcccag aaaacggaca
     6181 catccaattt gcagggcaat gcccagacgg ttgtgatcac ctctgctttt tccgataaat
     6241 tccttgtctg cttcatcaag gtgaaaatat cgtgccagct gaagctcatc cggttcaccg
     6301 gtgaatctgc catagctttc agtctgctca gtggtcagaa agtcaacggg catatcggcc
     6361 tccctgcctg acgggcattt agtaacattt ttccaaccgt acgaaatgtt ataaattatc
     6421 ggacatcgta aaactgttac attaatatgt ctattaaatc gtaaatttgt aataatagac
     6481 atgagttgtc cgatattcga tttaaggtac atttttatgc gactttttgg ttacgctcgg
     6541 gtctcaacca gtcagcagtc tcttgatctt caggtcagag cactcaaaga cgcaggtgtg
     6601 aaagcaaacc gtatatttac cgataaggca tccggcagtt caacagaccg ggaagggctg
     6661 gatttgctga ggatgaaggt ggaggaaggt gatgtcattc tggttaagaa gctcgaccgt
     6721 cttggccgcg acactgccga tatgatccaa ctgataaagg aatttgacgc tcagggcgtg
     6781 gcagtccggt tcattgatga cgggatcagt accgacggtg atatggggca aatggtggtc
     6841 accatcctgt cggctgtggc acaggctgaa cgccggagga tcctagaacg cacgaatgag
     6901 ggccgacagg aagcaaagct gaaaggaatc aaatttggcc gcaggcgtac cgtggacagg
     6961 aacgtcgtgc tgacgcttca tcagaagggc actggtgcaa cggaaattgc tcatcagctc
     7021 agtattgccc gctccacggt ttataaaatt cttgaagacg aaagggcctc gtgatacgcc
     7081 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     7141 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7201 cgctcatgag acaataaccc tggtaaatgc ttcaataata ttgaaaaagg aagagtatga
     7261 gtattcaaca ttttcgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7321 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7381 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7441 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggtgcggta ttatcccgtg
     7501 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7561 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7621 gtgctgccat aaccatgagt gataacactg ctgccaactt acttctgaca acgatcggag
     7681 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7741 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7801 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     7861 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     7921 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     7981 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8041 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8101 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8161 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8221 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccttcttc t
//
