LOCUS       NC_010885               4072 bp    DNA     circular CON 23-JUN-2024
DEFINITION  Escherichia coli plasmid pIGWZ12, complete sequence.
ACCESSION   NC_010885
VERSION     NC_010885.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224523
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Zaleski,P., Wolinowska,R., Strzezek,K., Lakomy,A. and
            Plucienniczak,A.
  TITLE     The complete sequence and segregational stability analysis of a new
            cryptic plasmid pIGWZ12 from a clinical strain of Escherichia coli
  JOURNAL   Plasmid 56 (3), 228-232 (2006)
   PUBMED   16828160
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Wolinowska,R., Zaleski,P., Strzezek,K. and Plucienniczak,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2005) Bioengineering, Institute of Biotechnology
            and Antibiotics, Staroscinska 5, Warsaw 02-516, Poland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009950625.1-RS_2024_06_23
            Annotation Date              :: 06/23/2024 04:16:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            DQ311641.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /plasmid="pIGWZ12"
     gene            complement(81..2240)
                     /gene="mobQ"
                     /locus_tag="HS384_RS00005"
                     /old_locus_tag="pIGWZ12_p1"
     CDS             complement(81..2240)
                     /gene="mobQ"
                     /locus_tag="HS384_RS00005"
                     /old_locus_tag="pIGWZ12_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029353.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            2419..2673
                     /gene="mobC"
                     /locus_tag="HS384_RS00010"
     CDS             2419..2673
                     /gene="mobC"
                     /locus_tag="HS384_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            3205..3897
                     /locus_tag="HS384_RS00015"
                     /old_locus_tag="pIGWZ12_p2"
     CDS             3205..3897
                     /locus_tag="HS384_RS00015"
                     /old_locus_tag="pIGWZ12_p2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281403.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVDELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(1883..2241)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          2242..2418
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3206..3897
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.965"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(85..2327)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.332"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcattcatcc ctgtcgatga acatagaaga acatcgacga acataacacg atgttaaaca
       61 aaacaaaata ttgttcacat tcactgtgac caaccgcgat catgtctcct ctcggtggtg
      121 tgtgttcttg catcttcctc gcgccgccat tcatcccagg tacgattgaa acccagagag
      181 cggaacagat gatccggctg tttttctggt gtgttgacgg cagcaaaaag cggtgcctca
      241 cgggttttca gatgaaactc cataagttcc ggcgcaggtg atatgtggct gtctgcatcg
      301 tgatttacgg caacaccata ccctgcatac cgtaacccct tatcggtcag tgcactgatg
      361 accagctggc tgttatcggc tgagggaacg gcaaacgcgg gatacagcgt accattaagc
      421 cggacaacac cgcccagaga gtcatccacg cccatgacca gcaaaagctg aagctggcgg
      481 gtttcccggt cgacagaact cattaccggc atcagcgtcg gtagcagacg tccctgcgta
      541 aaatccacct tccttgctgc cagtgatggc atggctggct ggcggtcttc tccaagacag
      601 aaagactccg gccatgtctt cggctggcgg agattgcggg tatattgcgg ccatgtctgg
      661 caaacctgcg tcagaagttg ctcaaaggtc gcagaggaat aaacacgttg cagtggtgga
      721 tgttgaccga tttcgcttcg gtcacagata taaccactcc tgtcacgcat taaatcaaca
      781 cgctcaaaga ttctggtgcg gtatttctcc agctcgttcc cccagagcgt gtattctccg
      841 ttccggttgt gaagcagaag gtaacaggtc atttccccgt taccaggcat gggggcctcg
      901 ccataccccg taacgactgc gctcagtacc cctgaatcca taacggcatt gtcatttttc
      961 ctgagctgtt ccgctcgtcg ctccgcgcga ctacgttctg tatcctcctg ctccttccgg
     1021 cgcaattcct cttcacgttt aagcaactgg actctgagag ctgcaaggcc ggatgccccc
     1081 cactgctgcc actgtttttc aaatgccgtc tgactcgcgg gaatgtcctg tttctccacc
     1141 ttccggtaat gctgtcgcaa tttttccatt tgctggctca gcgactggat ttttccctgt
     1201 tcgctacggt attccggtgc cgcccattgc atcaggggaa tacgggaaaa accggacgaa
     1261 aacggcgatg attttttcag gctggcagca tggctcatcg cttcctttcg ttcggtgttc
     1321 agctcttccc agcgtctgcg cgtcgcctgc atatctgcca tcatcgacat ggcattactg
     1381 gtgagtttcc atgccgcatt actgtccttc ggcaacgctt cgatgatgtc acgtagtgtg
     1441 tacgaagttg cgttgtcagg aagcacgata ttcagaaggt cagccacacg gcgggcaggc
     1501 tctgtcagcc cgctgttcct gagttcagtg cgggaaatct gtatttccag gttaagcctg
     1561 ttaatcagac gattctgttc gccgcgttct gtttcgatac cgcgtgtttc catcgcacag
     1621 gcagactttc cgaggtgaat accaggcgtt ttctcaagcc cctgtcgctc gtatgaacga
     1681 tggtctatct cttcctgata accggcgttc gccagtgctc tgtttgcatg gtcagcccat
     1741 gatgcacgcc acgtctcggc atgtgtccgg tcgttccagt ccctgacctt tccgccgaac
     1801 ccagcaggtc cgacagccct cgtggtcagc atgatgtgcg catggggatt tgttttatcc
     1861 atgtgatgga acgccacatc agcaatcatg ccctgactga caaagttttc tctgacaaag
     1921 gcgagaacgg tttcccgtgc ggcatcctgc ggcagttcac gggggatagc gagttcaatt
     1981 tcccgcgcaa gttgtgagtt cttccggcat tcggccttct caacggcatt ccagagttcg
     2041 gcacggttcg tacaccaggc aggggctgtc gcgggggtca gaatcacagc ttcagccaca
     2101 ccgcttttgc gggtgtagtc gtgagtcagt ccggtacgct catcaagcat cagtgaaccg
     2161 gaacgatagg cagcggaagc tacggcgctg tagccgctgc ttctggagat aattttcatg
     2221 ctgagatgat aaatcgccat ttctgccgta atgccttttt gtagttttct ttcagccccg
     2281 cagggcgcac acaatcgaaa cggagtggag attgtataag tgcgcacttt gtgatttgtc
     2341 gcgcattatg ccagctcagt ttttaaacta ccagtgacgc gctaaaatgc gatttttcat
     2401 cgcgcacgga gttacatcat gagcagaacg ctcgaacaga agattgctga tgctgaggcg
     2461 agattacagc gcctgaaggc gaagagccgg agcctggaca cagcgcagaa ggttgttgtg
     2521 ggtgcagcat tgctggcaaa ggtcagaaag ccggaggagg tgcagttgcg tgcctggtta
     2581 ctccagttcc tgaaggcaga ggtgacacga caggctgatg tgacgcgcat actgccgctg
     2641 attaacgagc tggaggcgct gccaggacag tgattgtatc gtccgctcgc cgcagcccca
     2701 gcgaccgagc gttagcgagc gagtgggcga ggaagcggaa gtgaatccgt cgccaggttg
     2761 agcacatacg cacggttttt cggtaggtga tatgcgctga acccgcatga atactggatt
     2821 tccacagggt tggaagggga gtattcggaa cttgcaattc tgctttcttg caacacattg
     2881 tttttattgg ttttttgttt tcagcgcccc aagttatcca caggctcgcc gttaagtttt
     2941 tagatcttgt ttttaagatc ttgtttttaa tacttgtttt agggtcaaaa ttcattaata
     3001 aaatcaatat gttatagagg tatatgtgga ggaatttaag gtaaaggtgg aggaatttaa
     3061 ggtgtttagg tggaggaatt taaggtaaag gtggaggaat ttaaggtgtt attcacagaa
     3121 atgtgaggat ataaaaaaaa atattccaca tttcttattt gtggggcaca ttaaagcatc
     3181 accacatttg aacgggatct ttgtatgtcg gacttgattg cctacaagtc gaatgctttg
     3241 gttgaggcca gctacaagtt gaccctacag gaacaacgtt ttctgttgct gtgtatcagt
     3301 cgattgaaat ctggtgctga tgctgaattg caaaagacaa tgaccattac cgctgcggaa
     3361 tattttgatt cattccctga catggggcgg aaaaatgccg aggttcagtt acaggaagcg
     3421 atcgatcgtt tatgggacag gtcgatcatt ctcaagaatg acgagaagcg tgaggagttc
     3481 cgctggatcc agtatcgggc acagtacgct aaaggtgagg ccaaggctcg aataaccttt
     3541 tctgacgcgg taatgcctta tctgacgcaa ctgaaggggc aattcacgcg cgttgttata
     3601 aaaaacatat caggtttatc aagtagttac agtataagaa tatacgagtt cctccagcaa
     3661 ttccgcagta cgggagagag aattattgct cttaatgact ttcgttcaat gttagggatt
     3721 gaaaataaat acaaacaatt cagagactta aataaaatac taataaaacc ctgtgttgac
     3781 gaactgaaca aaaaaagcga cctggctgta acggttgaga cgatcaaaaa ggggcgcacg
     3841 gttgtagccc tgcattttcg cttcaaggaa gacaaacaga tcaagatgac gatctaaaat
     3901 cgattttaag gcacctaaaa cgctccctgc tgcgtttttt ttgtgttagt agtgcgaagg
     3961 tactattgaa gtgaaaaatt gcgtcaaaag cgctctaatg cagcgaaaaa agaaccccca
     4021 atttttgggg gcggaaaaac aaaactatgc ttgcattatg tgtgtgcttc tt
//
