LOCUS       NC_010883               6750 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pIGMS5, complete sequence.
ACCESSION   NC_010883
VERSION     NC_010883.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225677
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Smorawinska,M. and Plucienniczak,A.
  TITLE     pIGMS5, a new plasmid from clinical Escherichia coli strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Smorawinska,M. and Plucienniczak,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2006) Bioengineering, Institute of Biotechnology
            and Antibiotics, Staroscinska 5, Warsaw 02-516, Poland
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009951205.1-RS_2024_06_24
            Annotation Date              :: 06/24/2024 02:58:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 6
            CDSs (total)                 :: 6
            Genes (coding)               :: 6
            CDSs (with protein)          :: 6
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            DQ916145.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /plasmid="pIGMS5"
                     /note="clinical strain"
     gene            complement(join(6598..6750,1..123))
                     /locus_tag="HS457_RS00005"
     CDS             complement(join(6598..6750,1..123))
                     /locus_tag="HS457_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091753.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            302..2521
                     /gene="mobQ"
                     /locus_tag="HS457_RS00010"
                     /old_locus_tag="-pIGMS5_p1"
     CDS             302..2521
                     /gene="mobQ"
                     /locus_tag="HS457_RS00010"
                     /old_locus_tag="-pIGMS5_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029327.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAQRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            2754..4499
                     /gene="col"
                     /locus_tag="HS457_RS00015"
                     /old_locus_tag="-pIGMS5_p2"
     CDS             2754..4499
                     /gene="col"
                     /locus_tag="HS457_RS00015"
                     /old_locus_tag="-pIGMS5_p2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_001347430.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFDRFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKA
                     TGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEVSKDPDLSKQ
                     FKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNIRVTTPKRHID
                     IHRGK"
                     /name="col"
     gene            4502..4762
                     /locus_tag="HS457_RS00020"
                     /old_locus_tag="-pIGMS5_p3"
     CDS             4502..4762
                     /locus_tag="HS457_RS00020"
                     /old_locus_tag="-pIGMS5_p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420693.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            4824..4967
                     /locus_tag="HS457_RS00025"
                     /old_locus_tag="-pIGMS5_p4"
     CDS             4824..4967
                     /locus_tag="HS457_RS00025"
                     /old_locus_tag="-pIGMS5_p4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            5497..6390
                     /locus_tag="HS457_RS00030"
                     /old_locus_tag="-pIGMS5_p5"
     CDS             5497..6390
                     /locus_tag="HS457_RS00030"
                     /old_locus_tag="-pIGMS5_p5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277131.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRMGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     BGC             1..6750
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(128..301)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(302..660)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5498..6390
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.387"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          303..2518
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.865"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctcgccagc atggcagccc ccacaatgac tttttgcgcc gtgtccaggc tccggctctt
       61 cgccttcagg cgctgtaatc tggcctcagc ttcggcaatc ttctgttcga gtgttctgct
      121 catttcgtga ctccgtgcgc ggtgaaaaat cgcattttag cgcgtcactg gtagtttaaa
      181 aactaaactg gcataatgca cggcacatca cgaagtgcgc acttatacaa tctccacttc
      241 gtttcgattg tgtgcggtct gcgacgctaa aagaaaacgg caaaaaggca ttacggcaga
      301 aatggcgatt tatcatctca gcatgaaaat catttcgcga aaaaacggct acagtgctgt
      361 tgcttctgct gcctaccgtt ccggctctgt catacccgat gaccgtaccg gattaatcca
      421 cgattacacc cgtaaacgcg gcgttgatga tgcggtcatt ctcacccctg cgaatgcacc
      481 gtcctggtgt ggtgaccgtt ccgttctctg gaatgcggtc gagaaagccg aacagcgacg
      541 gaactcccag ctggcaaggg agattgaact cgccattccc cgtgagattt cccgcgaggc
      601 cgcacgggag accgttctcg cttttgtccg ggaaaacttt gtcagtcggg gcatgattgc
      661 cgatgtggcg ttccatcaca tggaccggac caatccccat gcgcacatca tgctgaccac
      721 gagagctgtc ggggaaacgg gattcgcagg aaaggtcagg gactggaacg accgggcact
      781 cgccgagacg tggcgcgcat catgggctga ccatgcgaac agagcgcttg cgaacgccgg
      841 ctaccaggaa gagatagacc atcgttcata cgagcgtcag ggactggaga aagcgccggg
      901 ccttcacctc ggaaaggctg cctgtgcgat ggaaaaacgg ggaatggaaa cagaacgcgg
      961 tgagcagaac cgtctgatta acagccttaa cctggaaata caggtttccc gcacgcagct
     1021 tgctctcagg acggttcagg aagcgcagcg taagcgggaa ctcagcgatg ctgcacgtcg
     1081 tgcagcggaa gcccttaacc tgaccattcc cgctgcgaat gcctcagcgg ataccctgcg
     1141 ggaattcatt gccacgctgc cgcaggaatg cgggaacgcg tgggagatga ccccggagtt
     1201 cctggcgatg agcgggaagg tgaacgacat cgaacgtgag gggaatgcgc tgctgaaaga
     1261 gcaggccatt ctcgaaaagg agatgaccgg actcaaaaaa gcacgccctg tcgcgtccct
     1321 tctgtcagag attcccctga tgacatgggc tgaaccggaa taccgcaaaa gacaactccg
     1381 tttctggaaa ctcgggaaac agattgaatc tcttcgccgc acctacaggg ccgtgaaaga
     1441 acgggacatt cccgcccgtc gtcaggcctt tgaaacgcag tggaatacgt ggattgcgcc
     1501 gggaatggca gagctgaaag aaaaactgtc agcacgggaa gcggagcggc gcagggagga
     1561 agccgaagcg gaagcgcgcc ggaaggaaca ggagcatgag gcgcggctga aacgtcatga
     1621 taaccaccgt ctgagccgtg aaacggcatt agtcggggtt attacggagc tggggcgtgc
     1681 cagagagccg ggaacgggca ggataacccg ctacatgatg ctgagtaaca gagccggaga
     1741 attcacggta tggggtgatg agctggcgca ttacccccag agtgttcatg acccggtgaa
     1801 tgtttacctg tcgccaggcg gggctgtgat ggtctcggat atacgtgagg gaatgccaga
     1861 atctcatgag acgatggcgc ggcctgagcg tgtgagaatg tattccggtg cgacggtccg
     1921 gcatgtactg gaacagatgc gccaggggtg gccctcttac ggttttccgg cgctgccgca
     1981 tcactggccg gataattttt atttcagcga cgaccgcagg cccgtagcct ctccgctgcc
     2041 gtcagcgcag cgggtggata tcaccgctga tgctgtaccg gaacaactca tgcctgttgt
     2101 attttcgaga gagggaaaca gcaggacgct gaatctgttg ttatgcaaag ggccggagga
     2161 aatgcttgtc gggtttgttc gccgggaagg agggttttgc cccgttcttg tgcttccgtc
     2221 gccggattac agtcatctga tagtcagcac catcacggag aacggggtat gcctggcagg
     2281 ttacggagaa gctataaacc atgatgagga tactccgtac ccaccggaac cgcacctgat
     2341 gcagttccgg ctcaaaggcc atcatgacag gcttttggct gctgtccaca aaccggaaga
     2401 gatgccggat tatctcttcc gtcaactcgg ttttaatcag acctggcatg agtggaagcg
     2461 ggacgaacag cacaggcaac aacaacgccg ccccgggcac ttccggggca tgagtatgtg
     2521 atgtccgggg ctgcaccccg gaccccgcca acacatcacg ggccacaaaa ttttttgtgg
     2581 cccgctctgc gttttctaag tgttatccct cctgatttct aaaaaatttt ccacctgagc
     2641 ttgacagaaa aaacgatgac gggtactttt tgatctgtac ataaaaccag tggttttatg
     2701 tacagtatta atcatgtaat taattgtttt aacgcttaaa agagggaatt tttatgagcg
     2761 gtggcgatgg acgcggccat aacacgggcg cgcatagcac aagtggtaac attaatggtg
     2821 gcccgaccgg gcttggtgta ggtggtggtg cttctgatgg ctccggatgg agttcggaaa
     2881 ataacccgtg gggtggtggt tccggtagcg gcattcactg gggtggtggt tccggtcatg
     2941 gtaatggcgg ggggaatggt aattccggtg gtggttcggg aacaggcggt aatctgtcag
     3001 cagtagctgc gccagtggca tttggttttc cggcactttc cactccagga gctggcggtc
     3061 tggcggtcag tatttcagcg ggagcattat cggcagctat tgctgatatt atggctgccc
     3121 tgaaaggacc gtttaaattt ggtctttggg gggtggcttt atatggtgta ttgccatcac
     3181 aaatagcgaa agatgacccc aatatgatgt caaagattgt gacgtcatta cccgcagatg
     3241 atattactga atcacctgtc agttcattac ctctcgataa ggcaacagta aacgtaaatg
     3301 ttcgtgttgt tgatgatgta aaagacgaac gacagaatat ttcggttgtt tcaggtgttc
     3361 cgatgagtgt tccggtggtt gatgcaaaac ctaccgaacg tccaggtgtt tttacggcat
     3421 caattccagg tgcacctgtt ctgaatattt cagttaataa cagtacgcca gcagtacaga
     3481 cattaagccc aggtgttaca aataatactg ataaggatgt tcgcccggca ggatttactc
     3541 agggtggtaa taccagggat gcagttattc gattcccgaa ggacagcggt cataatgccg
     3601 tatatgtttc agtgagtgat gttcttagcc ctgaccaggt aaaacaacgt caggatgaag
     3661 aaaatcgccg tcagcaggaa tgggatgcta cgcatccggt tgaagcggct gagcgaaatt
     3721 atgaacgcgc gcgtgcagag ctgaatcagg caaatgaaga tgttgccaga aatcaggagc
     3781 gacaggctaa agctgttcag gtttataatt cgcgtaaaag cgaacttgat gcagcgaata
     3841 aaactcttgc tgatgcaata gctgaaataa aacaatttga tcgatttgcc catgacccaa
     3901 tggctggcgg tcacagaatg tggcaaatgg ccgggcttaa agcccagcgg gcgcagacgg
     3961 atgtaaataa taagcaggct gcatttgatg ctgctgcaaa agagaagtca gatgctgatg
     4021 ctgcattaag tgccgcgcag gagcgccgca aacagaagga aaataaagaa aaggacgcta
     4081 aggataaatt agataaggag agtaaacgga ataagccagg gaaggcgaca ggtaaaggta
     4141 aaccagttgg tgataaatgg ctggatgatg caggtaaaga ttcaggagcg ccaattccag
     4201 atcgcattgc tgataagttg cgtgataaag aatttaaaaa ctttgacgat ttccggaaga
     4261 aattctggga agaagtgtca aaagatcccg atcttagtaa gcaatttaaa ggcagtaata
     4321 agacgaacat tcaaaaggga aaagcacctt ttgcaaggaa gaaagaccaa gtaggtggta
     4381 gggaacgctt tgaattacat catgataaac caatcagtca ggatggtggt gtctatgata
     4441 tgaataatat cagagtgacc acacctaagc gacatattga tattcatcgg ggtaagtaaa
     4501 aatggaactg aaacatagta ttagtgatta taccgaggct gaatttctgg agtttgtaaa
     4561 aaaaatatgt agagctgaag gtgctactga agaggatgac aataaattag tgagagagtt
     4621 tgagcgatta actgagcacc cagatggttc agatctgatt tattatcctc gcgatgacag
     4681 ggaagatagt cctgaaggga ttgtcaagga aattaaagaa tggcgagctg ctaacggtaa
     4741 gtcaggattt aaacagggct gaaatatgaa tgccggttgt ttatggatga atggctggca
     4801 ttctttcaca acaaggagtc gttatgaaaa aaataacagg gattatttta ttgcttcttg
     4861 cagtcattat tctgtctgca tgtcaggcaa actatatccg ggatgttcag ggcgggaccg
     4921 tatctccgtc atcaacagct gaagtgaccg gattagcaac gcagtaaccc gaaatcctct
     4981 ttgacaaaaa caaagcgtgt caggctgatt ctgatgcgct ttttttttga aatgtcacaa
     5041 aaattccatg tgggagatgg gatctaaaat ccccgtgcag aactttccat ccagggggag
     5101 aaaacttgtc gttttgagcc gttcggtgtt cagaacgcac gaaaccgatc gcgcatcgct
     5161 ttcgtgcata gttatgcaac gcgctaaaaa cgattctgac gcatttttta tgtttaccct
     5221 ggtgtttcct tgtctttttg cgttttttgc gtcagaaagc gattgagggc gttttaaggg
     5281 gggcgtacaa cgggagttat ggtaaatgga tcgggttttc gggaaggatc gacaggattt
     5341 gccgttgggt gtagtgtaag cgactgaaaa acaaacgccc cgtaaatcgt gctctcaccg
     5401 ccaagattga tcacgaaatt acagggcgcc gggttccgcg tttcccgatg ggaaagcgcg
     5461 gttagttaaa ctgtgtaccg agagaaatcg tatcacatga gcgccgtact tcaacgcttc
     5521 agggaaaaat taccgcacaa accgtactgt acgaacgatt tcgcgtacgg cgttcgcatt
     5581 ctgccgaaaa acattgccat tcttgcccgt ttcatccagc agaaccagcc acatgcactg
     5641 tactggcttc cctttgacgt ggaccggatg ggggcatcaa tcgactggag cgaccggaat
     5701 tgtccggccc cgaacatcac cgtaaaaaat ccccgtaacg ggcacgcgca tctgctctac
     5761 gcgctcgccc ttcctgtgag aactgcgccg gatgcatcgg cttcggcgct cagatacgct
     5821 gccgctattg agcgtgcgtt gtgtgaaaaa ctgggcgcgg atgtgaatta cagcggcctg
     5881 atctgcaaaa atccgtgcca ccctgaatgg caggaagtgg aatggcgcga ggaaccctac
     5941 actctcgacg aactggctga ttatctcgat ttgagcgcct cagcgcgccg tagcgtcgat
     6001 aaaaattacg ggctggggcg aaactaccat ctgttcgaaa aggtccgtaa atgggcctac
     6061 agagcgattc gtcagggctg gcctgtattc tcacaatggc ttgatgccgt gatccagcgt
     6121 gtcgaaatgt acaacgcatc gcttcccgtt ccgctttctc cggctgaatg tcgggctatt
     6181 ggcaagagca ttgcgaaata tacacacagg aaattctcac cagagggatt ttccgctgta
     6241 caggccgctc gaggtcgcaa gggcggaact aaatctaagc gcgcagcagt tcctacatca
     6301 gcacgttcgc tgaagccgtg ggaggcatta gggatcagtc gagcgacgta ctaccgaaaa
     6361 ttaaaatgtg atccagatct cgcaaaatga gaccagataa gccttatcag ataacagcgc
     6421 ccttttggcg tctttttgag cagtgatctt tcttgcgatt ttctccgttc tgccccgtaa
     6481 tggcgtggct ttccgcatga ttgaggtggt agcgctcgcc gcagtctcat gaccgagcgt
     6541 agcgagcgag gaagcgcaaa ggcgtccggt ggtgcatggg cacttacgcg ccggggctta
     6601 gtggttctgc ggtttcgccg gtggtctggg tagcatctcc agctcgttaa tcagcggttg
     6661 tagtcggttc acatccgcct gtcttgtgac ttcctttcgc agaaactgga gcaggaacgc
     6721 acgcagttgc gcttcttccg gcctccgtac
//
