LOCUS       NC_010686               2412 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Staphylococcus aureus plasmid pKH20, complete sequence.
ACCESSION   NC_010686
VERSION     NC_010686.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225779
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Moon,K.H., Lee,J.Y. and Park,J.H.
  TITLE     Characterization of Antibiotic Resistance Plasmids of Staphylococcus
            aureus by Complete Nucleotide Sequence Determination
  JOURNAL   Yakhak Hoeji 52 (2), 147-150 (2008)
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Moon,K.H., Lee,J.Y. and Park,J.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2007) Pharmacy, Kyungsung University, 110-1,
            Daeyun-dong, Nam-gu, Busan 608-736, Korea
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 09/10/2023 03:43:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            EU350090.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="JY30"
                     /db_xref="taxon:1280"
                     /plasmid="pKH20"
     gene            389..865
                     /locus_tag="HS471_RS00005"
                     /old_locus_tag="pKH20_p1"
     CDS             389..865
                     /locus_tag="HS471_RS00005"
                     /old_locus_tag="pKH20_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            1499..1558
                     /gene="ermCL"
                     /locus_tag="HS471_RS00010"
     CDS             1499..1558
                     /gene="ermCL"
                     /locus_tag="HS471_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_002497446.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     gene            1619..2353
                     /gene="erm(C)"
                     /locus_tag="HS471_RS00015"
                     /old_locus_tag="pKH20_p2"
     ARG             1620..2350
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1620..2353
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          390..875
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atcgattatg tcttttgcgc attcacttct tttctatata aatatgagcg aagcgaataa
       61 gcgtcggaaa agcagcaaaa agtttccttt ttgctgttgg agcatggggg ttcagggggt
      121 gcagtatctg acgtcaatgc cgagcgaaag cgagccgaag ggtagcattt acgttagata
      181 accccctgat atgctccgac gctttatata gaaaagaaga ttcaactagg taaaatctta
      241 atataggttg agatgataag gtttataagg aatttgtttg ttctaatttt tcactcattt
      301 tgttctaatt tcttttaaca aatgttcttt tttttttaga acagttatga tatagttaga
      361 atagtttaaa ataaggagtg agaaaaagat gaaagaaaga tatggaacag tctataaagg
      421 ctctcagagg ctcatagacg aagaaagtgg agaagtcata gaggtagaca agttataccg
      481 taaacaaacg tctggtaact tcgtaaaggc atatatagtg caattaataa gtatgttaga
      541 tatgattggc ggaaaaaaac ttaaaatcgt taactatatc ctagataatg tccacttaag
      601 taacaataca atgatagcta caacaagaga aatagcaaaa gctacaggaa caagtctaca
      661 aacagtaata acaacactta aaatcttaga agaaggaaat attataaaaa gaaaaactgg
      721 agtattaatg ttaaaccctg aactactaat gagaggcgac gaccaaaaac aaaaatacct
      781 cttactcgaa tttgggaact ttgagcaaga ggcaaatgaa aaacaagaaa atgcattatc
      841 tgattattat tctttcaagg actagtataa cataaaatcg tctacaaata gacaaaaaac
      901 ctgcacgctt aatgtagatc aaaagcttaa cgcaaatgaa atagattgac ctcccaatat
      961 aagcgtgcag gtttaaagta cataaaaaat ataatgaaaa aaagcatcat tatactaacg
     1021 ttataccaac attatactct cattatacta attgcttatt ccaatttcct attggttgga
     1081 accaacaggc gttagtgtgt tgttgagttg gtactttcat gggattaatc ccatgaaacc
     1141 cccaaccaac tcgccaaagc tttggctaac acacacgcca ttccaaccaa tagttttctc
     1201 ggcataaagc catgctctga cgcttaaatg cactaatgcc ttaaaaaaac attaaagtct
     1261 aacacactag acttatttac ttcgtaatta agtcgttaaa ccgtgtgctc tacgaccaaa
     1321 agtataaaac ctttaagaac tttctttttt cttgtaaaaa aagaaactag ataaatctct
     1381 catatctttt attcaataat cgcatcagat tgcagtataa atttaacgat cactcatcat
     1441 gttcatattt atcagagctc gtgctataat tatactaatt ttataaggag gaaaaaatat
     1501 gggcattttt agtatttttg taatcagcac agttcattat caaccaaaca aaaaataagt
     1561 ggttataatg aatcgttaat aagcaaaatt cattataacc aaattaaaga gggttataat
     1621 gaacgagaaa aatataaaac acagtcaaaa ctttattact tcaaaacata atatagataa
     1681 aataatgaca aatataagat taaatgaaca tgataatatc tttgaaatcg gctcaggaaa
     1741 agggcatttt acccttgaat tagtacagag gtgtaatttc gtaactgcca ttgaaataga
     1801 ccataaatta tgcaaaacta cagaaaataa acttgttgat cacgataatt tccaagtttt
     1861 aaacaaggat atattgcagt ttaaatttcc taaaaaccaa tcctataaaa tatttggtaa
     1921 tataccttat aacataagta cggatataat acgcaaaatt gtttttgata gtatagctga
     1981 tgagatttat ttaatcgtgg aatacgggtt tgctaaaaga ttattaaata caaaacgctc
     2041 attggcatta tttttaatgg cagaagttga tatttctata ttaagtatgg ttccaagaga
     2101 atattttcat cctaaaccta aagtgaatag ctcacttatc agattaaata gaaaaaaatc
     2161 aagaatatca cacaaagata aacagaagta taattatttc gttatgaaat gggttaacaa
     2221 agaatacaag aaaatattta caaaaaatca atttaacaat tccttaaaac atgcaggaat
     2281 tgacgattta aacaatatta gctttgaaca attcttatct cttttcaata gctataaatt
     2341 atttaataag taagttaagg gatgcataaa ctgcatccct taacttgttt ttcgtgtacc
     2401 tattttttgt ga
//
