LOCUS       NC_010485               8909 bp    DNA     circular CON 01-JUN-2024
DEFINITION  Escherichia coli SMS-3-5 plasmid pSMS35_8, complete sequence.
ACCESSION   NC_010485
VERSION     NC_010485.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02604066
            Assembly: GCF_000019645.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli SMS-3-5
  ORGANISM  Escherichia coli SMS-3-5
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Fricke,W.F., Wright,M.S., Lindell,A.H., Harkins,D.M.,
            Baker-Austin,C., Ravel,J. and Stepanauskas,R.
  TITLE     Insights into the environmental resistance gene pool from the genome
            sequence of the multidrug-resistant environmental isolate
            Escherichia coli SMS-3-5
  JOURNAL   J Bacteriol 190 (20), 6779-6794 (2008)
   PUBMED   18708504
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Fricke,W.F., Wright,M.S., Lindell,A.H., Harkins,D.M.,
            Baker-Austin,C., Ravel,J. and Stepanauskas,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-MAR-2008) J. Craig Venter Institute, 9712 Medical
            Center Dr, Rockville, MD 20850, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000019645.1-RS_2024_06_01
            Annotation Date                   :: 06/01/2024 01:33:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,037
            CDSs (total)                      :: 4,920
            Genes (coding)                    :: 4,705
            CDSs (with protein)               :: 4,705
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 155 of 215
            Pseudo Genes (internal stop)      :: 48 of 215
            Pseudo Genes (multiple problems)  :: 64 of 215
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP000972.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Escherichia coli SMS-3-5"
                     /mol_type="genomic DNA"
                     /strain="SMS-3-5"
                     /db_xref="taxon:439855"
                     /plasmid="pSMS35_8"
     gene            complement(768..1001)
                     /locus_tag="ECSMS35_RS29405"
     CDS             complement(768..1001)
                     /locus_tag="ECSMS35_RS29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_029400396.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTEHLSQLYSESRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1008..1526)
                     /locus_tag="ECSMS35_RS29410"
     CDS             complement(1008..1526)
                     /locus_tag="ECSMS35_RS29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_029400393.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKVLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMRQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1564..2214)
                     /locus_tag="ECSMS35_RS30035"
                     /pseudo=""
     CDS             complement(<1564..2214)
                     /locus_tag="ECSMS35_RS30035"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2204..2527)
                     /locus_tag="ECSMS35_RS25750"
                     /old_locus_tag="EcSMS35_B0003"
     CDS             complement(2204..2527)
                     /locus_tag="ECSMS35_RS25750"
                     /old_locus_tag="EcSMS35_B0003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955996.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2591..2782
                     /locus_tag="ECSMS35_RS25755"
                     /old_locus_tag="EcSMS35_B0004"
     CDS             2591..2782
                     /locus_tag="ECSMS35_RS25755"
                     /old_locus_tag="EcSMS35_B0004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3452..3610
                     /locus_tag="ECSMS35_RS29590"
     CDS             3452..3610
                     /locus_tag="ECSMS35_RS29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354639.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAAVGNLVSRAMMA
                     QMVQQSS"
                     /name="hypothetical protein"
     gene            3992..4333
                     /locus_tag="ECSMS35_RS25760"
                     /old_locus_tag="EcSMS35_B0008"
     CDS             3992..4333
                     /locus_tag="ECSMS35_RS25760"
                     /old_locus_tag="EcSMS35_B0008"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="WP_000058758.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNNEGYYFLASDKMLYAIVI
                     STILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 family microcin immunity protein"
     gene            complement(4330..5895)
                     /locus_tag="ECSMS35_RS25765"
                     /old_locus_tag="EcSMS35_B0007"
     CDS             complement(4330..5895)
                     /locus_tag="ECSMS35_RS25765"
                     /old_locus_tag="EcSMS35_B0007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_000447001.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEAEEKRLAALNEEARAVEVAQKNLAAAQSELAKVDEEIKTLNTRLSSSI
                     HARDAEMNTLSGKRNELAQASAKYKELDELVKKLSPRANDPLQSRPFFYATSRRTGAGK
                     IMEEKQKQVTASETRINQLNAEINGIQGAMSQANNNRNTAVLRVHESEENLKTAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKTVDAGVSYIVVLLFSVLAGTTLGIWGIAIVTGILCAFIDKNKLNTIND
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            6173..6592
                     /locus_tag="ECSMS35_RS25770"
                     /old_locus_tag="EcSMS35_B0009"
     CDS             6173..6592
                     /locus_tag="ECSMS35_RS25770"
                     /old_locus_tag="EcSMS35_B0009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312320.1"
                     /translation="MAWHTLQNALKMTGRSRSQFYRDMRAGLVSYRTGQDGRREFETSE
                     LIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELRQCLTLMLEDKQAQATERR
                     RQEAEREQLHKEIEQLRQALEQEKKRGFWARLFSR"
                     /name="hypothetical protein"
     gene            6676..7095
                     /locus_tag="ECSMS35_RS25775"
                     /old_locus_tag="EcSMS35_B0010"
     CDS             6676..7095
                     /locus_tag="ECSMS35_RS25775"
                     /old_locus_tag="EcSMS35_B0010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254598.1"
                     /translation="MRRSGGTLYLIALLTAATVLTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7621..>7797
                     /locus_tag="ECSMS35_RS25785"
                     /old_locus_tag="EcSMS35_B0011"
                     /pseudo=""
     CDS             7621..>7797
                     /locus_tag="ECSMS35_RS25785"
                     /old_locus_tag="EcSMS35_B0011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     Typing          complement(2868..2949)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          2978..3524
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.841"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(665..2214)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.893"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaaagaata atattctaaa ctacagttat ttatgcagaa cataacatca aactcacaat
       61 aaatataaaa caattatttt ataactgttc acttattata ttcagacata tcagaaacac
      121 gactcaattc ttttttattt aacaaaatcc ataaaaaact tatggttgaa atcacattta
      181 aactgtgaag gatatcatcc ggagaaattt ttcaatccga cactccatag aatggacctt
      241 taagacttta tcctcatcat gtatgaggat tttttttctg gcaaacagta tctttcagta
      301 atccttgctc agttccgatt ctgacgtatc cccgtgtgga tctgaaaagg agataattac
      361 gctctgtggc caacaggaat aactcaagca gctcctctct gataccatca gaagaagcat
      421 taaacgcatt ctggcgtcga ttacagaaga agcggaacgc gttccaggct cctgttgcta
      481 tacacactac ccacactaac cccatggttt ttgagcgcca gaaacgcgtc cgtatgcgtt
      541 ttaacgcgtt cctgtgtttt cagcaaggcc tgactgaagc tagttcctag tgccctgaac
      601 caaccattat ccaaaaaaat gcaccggaaa cgcgctgaca acactttagc ctgtctttgg
      661 ttaccatccc agcgacgggc catccagtgt ccgttcctgt atttccagat ggcgctcacg
      721 ctctaggaat agctcatgtt cacgttcctg tatcagtagt tcgtccctta tctcctgctc
      781 tcgctgtata actggctcaa gtgttctgtc tgctcgctca agtgctgcac ctgctgactc
      841 aactgcatga cccgctcgtt cagcatcgcg ttgtcccgtt gcgtaagcga aaacatcttc
      901 tgcaattcca cgaaggcgct ctcccattcg ttcagccgct gcatatagtc ctgttgcagc
      961 tgctctaagg cgttcagcaa atgtgtttcc agctctgtca ctctgtgtca ctccttcaga
     1021 tgtacccact ctttcccctg aaagggaatc acctctgctg atttcccgta cggaaggacc
     1081 agaaatttcc tgttcccgtc ctgcacgaac tccacgcccc atgtcttcgc gttcagtttc
     1141 tgcaatgtct cttcctgctt cctgatatct tccaggttcg cctgtatcct cccgccaaga
     1201 taccagagcg tcccgccact cgcggtaaac aggagaaaga ctatccccag taacatcatg
     1261 cccgtattcc ctgccagctt taacacgtcc ttcctgtgct gcctcatcgc ctctttcacc
     1321 ccttcccggt gtttctccag cgattcctct gtcgaggctg tgaacagggc tatagcgtct
     1381 ctgattttcg tctcgtttga tgtcacagcc tcgcttacag attcgccgag cctcctgaac
     1441 tcgttgttca gcactttctc tgtagattcg gctctctctt tcagcttttt ctcgaactcc
     1501 gcgcccgtct gtaaaagatt gctcataaaa cgctcctttc agcctgatat tcttcccgcc
     1561 gttcgggtct gcaatgctga tactgcttcg cgtcaccctg acaacctcaa ctcccacatc
     1621 tgtgagtgcc tgaatcacat cctgacggtc ttttatctct ccggcacggt aaagcacatc
     1681 aagcccccgt gtaacgctct cagcaatcgc ctgtttcgtt tccggcaggt tatcagggag
     1741 tgtcagcgtc ctgcggttct ccggggcatt cgggtcatgc agcccgtaat ggtgattcac
     1801 cagcgtctgc caggcatcaa ttctcggcct gtctgcgcgg tcatagtacg gctggaggcg
     1861 ttttccgctc tgtagctcca tgttcggaat gacaaaattc agctcaagcc gtcccttgtc
     1921 ctggtgctca acccacagga tgctgtactg atttttttcg agaccgggca tcagtacacg
     1981 ctcaaagctc gccatcactt tttcacgtcc tcccggcggc agctccttct ccgcgaacga
     2041 cagaacacct gacgtgtatt tcttcgcaaa tggcgtggca tcgatgagtt cccggacttc
     2101 ttccggatta ccctgaagca ccgttgcccc ttcccggtta cgctcccttc ccagcaggta
     2161 atcaaccgga ccactgccac cgccttttcc cctggcatga aatttaacta tcatcccgcg
     2221 ctccctgttc cctgacgacc tgccgtaagc tgcgcaactc tctctcgatg gccatcagtg
     2281 cggccaccac ctgaacccgg tcaccggaag accactgccc gctgttcacc ttcctcgccg
     2341 tctgattcag gttattcccg atggcagcca gctgacgcag taacggcggt gccagtgtcg
     2401 gcagcttccc ggaacgggca accggctcac ccaggcatac ccgccgcatc cataccgcca
     2461 gttgtttacc ctcacagcgt tcaagtaacc tggcatgttc atcatcagta acccgtatcg
     2521 tgagcatcct ctcgcgtttc atcggtatca ttaccccatg aacagaaatc ccccttacac
     2581 ggaggcatca gtgactaaac aggaaaaaac agcccttaac atggcccgct ttatcagaag
     2641 ccagacatta acgctgctgg aaaagctcaa cgaactggac gcggatgaac aggcagacat
     2701 ctgtgaatcg cttcacgacc acgccgatga gctttaccgc agctgcctcg cacgtttcgg
     2761 ggatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgtg
     2821 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcaggtttta gggggtgtcg
     2881 gggcgcagcc ctgacccagt cacgtagcga tagcggagtg tatactggct tagtcatgcg
     2941 gcatcggtgc ggattgtatg aaaagtgcac catgtacggt gtgaaatgcc gcacagatgc
     3001 gtaaggagaa catgcagatg ccgatgctct tccgcttcct cgctcactga ctcgctgcgc
     3061 tcggtcgttc ggctgtggcg agcggtgtct gctcactcaa aagcggtgat actgttatcc
     3121 acacaatcag gggataacgc cggaaagaac atgtgagcaa aaaacgaaga ccccagaaaa
     3181 ggccgcgccg gaggcgcttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3241 gacgctcaag tcagaggtgg cgaaacccga caggacttaa agataccagg cgtttccccc
     3301 cggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctctccgc
     3361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcagttc
     3421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acaaagcgcc
     3541 actggcagca gccgttggta acttggttag cagagcgatg atggcgcaaa tggtgcaaca
     3601 gagttcttga agtagtggcc cgactacggc tacactggaa ggacagtatt tggtatctgc
     3661 gctctgctga agccagttac ctcggttaag cagttcccca actgacttaa ccttcgatca
     3721 aaccgcctcc ccaggcggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg
     3781 atctcaagaa gatcctttga tcttttctac tgaacagaga tccccgtcag tttagaagaa
     3841 gatgatggag ccacagtccc tccctgaaca tcacgtatat agttagcctg acatccaaca
     3901 aggaggttta tagctaatat tcccacaaaa aatcttttcc tcataactcg atccttataa
     3961 aatgaaaaga atatataacg aggtttaatt tatgagttta agatactaca taaaaaacat
     4021 tttgtttggc ctatattgca cacttatata tatatacctt ataacaaaaa acaacgaagg
     4081 gtattatttc ctagcgtcag ataagatgct atacgcaatc gtgataagca ctattctatg
     4141 cccatattca aaatatgcta ttgaacacat attttttaag ttcataaaga aagatttttt
     4201 cagaaaaaga aaaaatctaa ataatgcccc cgtggcaaaa ttaaacctat ttatgctata
     4261 caatctactt tgtttggtcc tagcaatccc atttggattg ctaggactgt ttatatcaat
     4321 aaagaataat taaataccca acacatcatt tatagtatta agtttattct tgtcaataaa
     4381 ggcacataga atgcctgtaa caatagcaat cccccagata cctaatgtag ttccagcaag
     4441 cacactaaaa agtaaaacaa caatataact aactccagca tctacagttt tcttctctaa
     4501 tgtaagaaat agtggcttcc agtcacctgt atccttaatt tttaggatat cagataccac
     4561 atcatatcca aaagaaacat gtcccgtaat cttcaagtac ttagcaaact gatctaaatg
     4621 cttagcccag tcttcatact taaccgattc cagtgcattg aaaatcgcat cacggtctgc
     4681 tttgctgaat ttcttattta aaacatcctt gtatttttca aaagcagcta gagcttcatt
     4741 cacattgctg attttcttac ctttagactt atcagcaagt tcctgtgcca tttttgaata
     4801 tttttcacca tatttttcag atagcgtttg ataaaagcta actgttgcat ccacagcatc
     4861 cttaatctgc gagttcagga gattagtctg cgctgttttc aaattttctt cagattcatg
     4921 aacacgtaaa acagctgtat tacgattatt attagcctga gacatagccc cctgaattcc
     4981 atttatctca gcattaagtt ggttaatacg cgtttctgat gctgttacct gtttttgttt
     5041 ttcctccata atcttaccgg ctcccgtccg tcgactggtg gcatagaaaa aaggacgact
     5101 ctgaagcgga tcattagctc ttggtgacaa ttttttgacc agttcatcaa gttccttata
     5161 tttagcagat gcctgagcca gctcatttcg ttttccggac agcgtattca tttctgcatc
     5221 acgggcatga atactggagc ttaaacgggt attgagagtc ttaatctctt catccacttt
     5281 cgccagctca gattgtgcag cagcaagatt tttttgtgcc acctccactg cccgggcctc
     5341 ttcattcaat gctgccagcc gtttctcttc agcttcagcc agtttcaact ggcgttctgt
     5401 ttcagcctgc tccttctcta tctctttacg tcgttgttct gcctcctgaa aagccttttc
     5461 cgcagcttcc gcttctttac gggctttttc ttctgctttt gcaaggcgca accgctctgc
     5521 ttctgcctgc atcgctgcat tattggcatg agccaggtca ataacctccg gatgagtgga
     5581 attatgacga agcgcctcat tcacaatatc cttcagatgt tgagtcagcg catcccggtt
     5641 tgcttttgct ttagcctgtg cttctgcggc agcttttgcc cgggcagcct gttctgcctg
     5701 cgttttcttc aattgagcag tagaccattt agctgtggca tgaatggctg cagaactttc
     5761 acttttgcta cctccagttc caccaccgcc gccagagcca ctcccgtctg gattaccatt
     5821 caaaagagta atgattacct gccccttatc atcataagga acaccatctt tatagtacgc
     5881 tacagctgtt tccattataa aatcctcttt gacattaaaa acaaatagtt aaaagcaata
     5941 tactgtatat ataaccactg gttttatata cagcttaaaa ttacgccgct gcattttccc
     6001 tgtcaaccct gtggattttc atttttgtga aaacgatcaa aaaaacagcc acccacacca
     6061 cgtgctattc ccacccgatg ccacaaaaac cagcacaaac attaccgttc tccggcccca
     6121 gtatgtttta ctgaaaccat gatatgagac attaatggga cactgtcact tcatggcatg
     6181 gcacacactc cagaacgcac tgaaaatgac aggcagatcg cgttcacagt tttaccgtga
     6241 tatgcgcgca ggccttgtca gttatcgtac aggccaggac ggacggcggg agtttgaaac
     6301 cagtgaactg atccgggcat acggcgaatt aaagcaggat gagacaccag aaaggcacag
     6361 tgcgggacag gcagaaaatc cacatgatca gcagacagaa cgcattctcc gggagctgaa
     6421 tgagctgaga caatgcctga cactgatgct tgaggacaaa caggcacagg ctacggaacg
     6481 ccggcgccag gaagccgaac gggaacagct acataaagag atagagcagc tcaggcaggc
     6541 actggagcag gaaaaaaaac ggggattctg ggccagactg ttcagccggt gagcaccgac
     6601 tgcaagtgat gataagatag tcagcggtta aatataagtc actgagtaca aagatttaaa
     6661 ggtaaggaga cactcatgag acgttccggc ggcacactgt atcttatcgc cctgctgaca
     6721 gcggcaacgg tcctgacagc ctgcacgcca aagggcagtg tggaacaaca tacccggcat
     6781 tacgtttatg catcagacga tggttttgat cctaactttt ccacccagaa ggccgacaca
     6841 acacgaatga tggtgccttt tttccggcag ttctgggata tgggagcaaa ggataaagcg
     6901 acaggaaaat cacggagtga tgtgcagcaa cgcattcagc agtttcacag ccaggaattt
     6961 ttaaactcac tccggggcac aactcaattt gcgggtactg attaccgcag caaagacctt
     7021 accccgaaaa aatccaggct gctggctgag acgatttctg cggtttatct cgatggctac
     7081 gagggaagac agtaagtgga tttaccataa tcccttaatt gtacgcaccg ctgaaatgca
     7141 ttcagcgcaa tcacggcagc agacaggtaa aaatcgcaac aacccccccg aaaaactacc
     7201 gcgatcgcgc ctgataaatt ttaaccttat gcatacctat gcatccaggt gaatcacaag
     7261 cgaatttcct tactgagacg actgaaacgg ttttttttca caattttcag ctgttttcga
     7321 ttccacaaaa tagaagttac aggataggga ttaatcccct tcgccgccaa ggggagcagg
     7381 atgagtcagg ggaatgtcta ttcacctgcg ctgtacaggc tgccaggtaa aagtattcgc
     7441 tgcggcacga tactcttttg ccctcatagc ctttgcgctt cttacttttc gttcttccag
     7501 aatcattctt aagacatttt cccgcacttt tctctaactt cccgtggcaa tttatctctg
     7561 ccggactgaa ccgccccgga aatcctggag actaaacttc ctgagaaaga tgtaaacagg
     7621 atgactaaaa atactcgttt ttcccccgaa gtccgtcaac gggcagttcg tatggttctg
     7681 gaaagccagg gcgaatatga ctcacaatgg gcggcaattt gttccattgc tccaaagatt
     7741 ggctgtacgc cagagaccct gcgtgtctgg gttcgccagc atgagcggga taccgggtat
     7801 tacctttcga agaaattttt gacatcctct acgccctgaa ggaccgggtt ttacggcgta
     7861 ccagataaaa aaacccctct actgaagagg ggtagtgtaa ggaacttgct gattttcttg
     7921 atattattaa ccatcaataa ctgagcatac aaaaaataac aaaactctga cacagataca
     7981 accatccctc tttgtataag accgatgtgc atctcaaaaa agaagcgtgt ttctggaaca
     8041 cagaacaaca atgcacattg tagccaacaa attcacacca gaaacacgct ctgtttttta
     8101 atttacacaa gaatatatgt ctggaaaatc aggaagaaga aattaatggg cattctgtat
     8161 acgaagggag agcgcgtttc cggtgcccca tattagatat tatccctgca acaaacacca
     8221 gagacgcgct cataattctt ctagcgtaaa tgatcctttc aggcaagaaa aaagccgtga
     8281 ttctgtatca cggccttaat tttatcgaaa acacggattt tagtatgcac atgactgtac
     8341 tcctgtggag tacaaggaaa tcataatcag cgaccaaacc atttatcaac agaaaaccaa
     8401 catattgatg ttatttttgt gacaaatgtt acaaattcga aaccactgta tttttactgc
     8461 aacaaaatct ggttgatcta caagttacac tggggtaaaa agttgtccgg aatgtttctg
     8521 cgtagtaaag cctctagccg gattgccttt gcaaaaacgc aggaacgttc cccccttcat
     8581 cagtactcct gacgctgtaa ggagctgact gatgcagaca gggctgatga tatctaataa
     8641 aagctatctc atttttgcgc gtacataaaa taaatcaggc acttccctac actccccctc
     8701 cggaattgag ggggttttct ttacaatgta aataaattgc ttgcgtaaag ttcaaaaaaa
     8761 ctgcaaattt ttactgtagt gtcgagcttc atgtagctca ccagggcatg gactaaaaag
     8821 aagtaaaccc atcttttacc cccccttata cggggggttc tttatagcac aaagaacact
     8881 tataatttat catctcacag ctataaata
//
