LOCUS       NC_010262               4442 bp    DNA     circular CON 10-SEP-2023
DEFINITION  Staphylococcus aureus plasmid pKH16, complete sequence.
ACCESSION   NC_010262
VERSION     NC_010262.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225774
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Moon,K.H., Park,J.H. and Lee,J.Y.
  TITLE     Characterization of Tetracycline Resistance Plasmids of
            Staphylococcus aureus
  JOURNAL   Yakhakhoe Chi 52 (4), 279-282 (2008)
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Moon,K.H., Park,J.H. and Lee,J.Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-DEC-2007) Pharmacy, Kyungsung University, 110-1,
            Daeyun-dong, Nam-gu, Busan 608-736, Korea
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 09/10/2023 03:39:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            EU365621.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="JY10"
                     /db_xref="taxon:1280"
                     /plasmid="pKH16"
                     /note="pT181 family plasmid"
     gene            join(3657..4442,1..159)
                     /locus_tag="HS472_RS00005"
                     /old_locus_tag="pKH16_01"
     CDS             join(3657..4442,1..159)
                     /locus_tag="HS472_RS00005"
                     /old_locus_tag="pKH16_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            321..1700
                     /gene="tet(K)"
                     /locus_tag="HS472_RS00010"
                     /old_locus_tag="pKH16_02"
     ARG             322..1697
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1889..3130
                     /gene="mobV"
                     /locus_tag="HS472_RS00015"
                     /old_locus_tag="pKH16_03"
     CDS             1889..3130
                     /gene="mobV"
                     /locus_tag="HS472_RS00015"
                     /old_locus_tag="pKH16_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000122374.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQETGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     Typing          3658..4442
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1890..3127
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcttcaca gaaattctag aacaaaatat aagaatttga taaaagaaat ttcgccagtc
       61 gatttaacgg acttaatgaa atcgacttta aaagcgaacg aaaaacaatt gcaaaaacaa
      121 atcgattttt ggcaacatga atttaaattt tggaaatagt gtacatatta atattactga
      181 acaaaaatga tatatttaaa ctattctaat ttaggaggat ttttttatga agtgtctatt
      241 taaaaatttg gggaatttat atgaggtgaa agaataattt acccctataa actttagtca
      301 cctcaagtaa agaggtaaaa ttgtttagtt tatataaaaa atttaaaggt ttgttttata
      361 gcgttttatt ttggctttgt attctttcat tttttagtgt attaaatgaa atggttttaa
      421 atgtttcttt acctgatatt gcaaatcatt ttaatactac tcctggaatt acaaactggg
      481 taaacactgc atatatgtta actttttcga taggaacagc agtatatgga aaattatctg
      541 attatataaa tataaaaaaa ttgttaatta ttggtattag tttgagctgt cttggttcat
      601 tgattgcttt tattggtcac aatcactttt ttattttgat ttttggtagg ttagtacaag
      661 gagtaggatc tgctgcattc ccttcactga ttatggtggt tgtagctaga aatattacaa
      721 gaaaaaaaca aggcaaagcc tttggtttta taggatcaat tgtagcttta ggtgaagggt
      781 taggtccttc aataggggga ataatagcac attatattca ttggtcttac ctacttatac
      841 ttcctatgat tacaatagta actatacctt ttcttattaa agtaatggta cctggtaaat
      901 caacaaaaaa tacattagat atcgtaggta ttgttttaat gtctataagt attatatgtt
      961 ttatgttatt tacgacaaat tataattgga cttttttaat actcttcaca atcttttttg
     1021 tgatttttat taaacatatt tcaagagttt ctaacccttt tattaatcct aaactaggga
     1081 aaaacattcc gtttatgctt ggtttgtttt ctggtgggct aatattttct atagtagctg
     1141 gttttatatc aatggtgcct tatatgatga aaactattta tcatgtaaat gtagcgacaa
     1201 taggtaatag tgttattttt cctggaacca tgagtgttat tgtttttggt tattttggtg
     1261 gttttttagt ggatagaaaa ggatcattat ttgtttttat tttaggatca ttgtctatct
     1321 ctataagttt tttaactatt gcattttttg ttgagtttag tatgtggttg actactttta
     1381 tgtttatatt tgttatgggc ggattatctt ttactaaaac agttatatca aaaatagtat
     1441 caagtagtct ttctgaagaa gaagttgctt ctggaatgag tttgctaaat ttcacaagtt
     1501 ttttatcaga gggaacaggt atagcaattg taggaggttt attgtcacta caattgatta
     1561 atcgtaaact agttctggaa tttataaatt attcttctgg agtgtatagt aatattcttg
     1621 tagccatggc tatccttatt attttatgtt gtcttttgac gattattgta tttaaacgtt
     1681 ctgaaaagca gtttgaatag ttatattata ttttggttta gaactatgag tggctagcat
     1741 tttgccactc attttttgcg ttagcaaaaa caagtttaag cctcgcagag cacacgtatt
     1801 aacgacttat taaaaataag tctagtgtgt tattagactt aaactattaa atacacatga
     1861 aacctttgtg cttaggagtg atttttatat gtcttattcc attgttagag tttcaaaagt
     1921 taaatctgga acaaatacaa cgggcataca aaaacatgtt caaagagaaa ataataatta
     1981 tgaaaatgaa gatatagacc atagtaaaac ttacttaaat tatgatttgg taaatgctaa
     2041 taaacagaat tttaataact tgattgatga aaaaatcgaa cagaattata caggcaaaag
     2101 aaaaattaga acagacgcga ttaaacacat tgatggttta attacatcag acaatgattt
     2161 ctttgataat caaacgccag aagatacaaa gcagtttttt gaatatgcta aagagttttt
     2221 agaacaagaa tacggtaaag ataatttatt atatgcaaca gttcacatgg acgaaaaaac
     2281 accacatatg cattatggcg ttgttccaat aactgatgat ggtcgtttaa gtgctaaaga
     2341 agttgtaggt aataaaaaag ctttaacagc gtttcaagat agatttaatg agcatgttaa
     2401 acaacgagga tatgatttag aacgtgggca atcaagacaa gtaacaaatg ctaaacatga
     2461 gcaaataagt cagtataaac aaaaaacaga atatcataag caagaatatg aacgtgagag
     2521 ccaaaaaaca gaccatataa agcaaaagaa cgataaatta atgcaagagt accaaaaatc
     2581 gttaaatacg cttaaaaagc ctataaatgt tccgtatgag caagaaactg aaaaagtagg
     2641 tggtttattt agcaaagaaa tacaagaaac tggaaatgtt gtaataagcc aaaaagattt
     2701 caatgaattt cagaaacaga taaaagctgc tcaagatatt tcggaagatt acgagtatat
     2761 aaagtctggt agagccttag atgataaaga taaggaaata cgagagaaag atgatttatt
     2821 aaataaagca gttgagcgta ttgaaaacgc agacgataat tttaaccaac tttacgaaaa
     2881 tgcaaagcca cttaaagaga atatagaaat agcgttaaag cttttaaaaa tcttactaaa
     2941 agagttagaa cgagttttag gaagaaatac ctttgcggaa agagttaata agttaacaga
     3001 agatgaacca aaactaaatg gtttagcagg aaacttagat aaaaaaatga atccagaatt
     3061 atattcagaa caggaacagc aacaagaaca acaaaagaat caaaaacgag atagaggtat
     3121 gcacttatag aacatgcatt tatgccgaga aaacttattg gttggaatgg gctatgtgtt
     3181 agctaacttg ttagcgagtt ggttggactt gaattgggat taatcccaag aaagtaccaa
     3241 ctcaacaaca cataaagccc tgtaggttcc gaccaataag gaaattggaa taaagcaata
     3301 aaaggagttg aagaaatgaa attcagagaa gcctttgaga attttataac aagtaagtat
     3361 gtacttggtg ttttagtagt tttaactgtt taccagataa tacaaatgct taaataaaaa
     3421 aagacttgat ctgattagac caagtctttt gatagtgtta tattaataac aaaataaaaa
     3481 ggagtcgctc acgccctgac caaagtttgt gaacgacatc attcaaagaa aaaaacactg
     3541 agttgttttt ataatcttgt atatttagat attaaacgat atttaaatat gcatcaagat
     3601 atatatttgg gtgagcgatt ccttaaacga aattgagatt aaggagtcga ttttttatgt
     3661 ataaaaacaa tcatgcaaat cattcaaatc atttggaaaa tcacgattta gacaattttt
     3721 ctaaaaccgg ctactctaat agccggttgg acgcacatac tgtgtgcata tctgatccaa
     3781 aattaagttt tgatgcaatg acgatcgttg gaaatctcaa ccgagacaac gctcaagccc
     3841 tttctaaatt tatgagtgta gagccccaaa taagactttg ggatattctt caaacaaagt
     3901 ttaaagctaa agcacttcaa gaaaaagttt atattgaata tgacaaagtg aaagcagata
     3961 gttgggatag acgtaatatg cgtattgaat ttaatccaaa caaacttaca cgagatgaaa
     4021 tgatttggtt aaaacaaaat ataataagct acatggaaga tgacggtttt acaagattag
     4081 atttagcctt tgattttgaa gatgatttga gtgactacta tgcaatgtct gataaagcag
     4141 ttaagaaaac tattttttat ggtcgtaatg gtaagccaga aacaaaatat tttggcgtga
     4201 gagatagtaa tagatttatt agaatttata ataaaaagca agaacgtaaa gataatgcag
     4261 atgctgaagt tatgtctgaa catttatggc gtgtagaaat cgaacttaaa agagatatgg
     4321 tggattactg gaatgattgc tttagtgatt tacatatctt gcaaccagat tggaaaacta
     4381 tccaacgcac tgcggataga gcaatagttt ttatgttatt gagtgatgaa gaagaatggg
     4441 ga
//
