LOCUS       NC_009794               5601 bp    DNA     circular CON 22-APR-2024
DEFINITION  Citrobacter koseri ATCC BAA-895 plasmid pCKO2, complete sequence.
ACCESSION   NC_009794
VERSION     NC_009794.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603912
            Assembly: GCF_000018045.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri ATCC BAA-895
  ORGANISM  Citrobacter koseri ATCC BAA-895
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   McClelland,M., Sanderson,E.K., Porwollik,S., Spieth,J.,
            Clifton,W.S., Latreille,P., Courtney,L., Wang,C., Pepin,K.,
            Bhonagiri,V., Nash,W., Johnson,M., Thiruvilangam,P. and Wilson,R.
  CONSRTM   The Citrobacter koseri Genome Sequencing Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2007) Genetics, Genome Sequencing Center, 4444
            Forest Park Parkway, St. Louis, MO 63108, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000018045.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 01:03:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,418
            CDSs (total)                      :: 4,307
            Genes (coding)                    :: 4,254
            CDSs (with protein)               :: 4,254
            Genes (RNA)                       :: 111
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 25 of 53
            Pseudo Genes (incomplete)         :: 26 of 53
            Pseudo Genes (internal stop)      :: 12 of 53
            Pseudo Genes (multiple problems)  :: 9 of 53
            Pseudo Genes (short protein)      :: 1 of 53
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP000824.1.
            Citrobacter (diversus) koseri--Citrobacter cells are isolated from
            water, sewage, soils, and food, as well as from the feces of man
            and other animals, where they may be normal inhabitants. They can
            be found in urine, sputum, and other clinical specimens. They can
            sometimes be opportunistic pathogens particularly in
            immunocompromised patients in hospitals or in infants (Pepperell et
            al., Antimicrob Agents Chemother. 2002 Nov;46(11):3555-60. and
            references therein).
            The strain of Citrobacter koseri being sequenced, strain CDC
            4225-83, was isolated in 1983 in Maryland, where it caused neonatal
            meningitis. It was provided by Caroline Mohr and Melissa Campbell
            of CDC. The strain is available from the American Type Culture
            Collection as ATCC BAA-895 or from the Salmonella Genetic Stock
            Centre as SGSC4696. The genome was sequenced to 8X coverage, using
            plasmid and fosmid libraries and was finished to an error rate of
            less than 1 per 10,000 bases. Automated annotation was performed
            and manual annotation will continue in the labs of Michael
            McClelland and Kenneth Sanderson. The National Institute of Allergy
            and Infectious Diseases (NIAID), National Institutes of Health
            (NIH) has funded this project.
            Coding sequences below are predicted using GeneMark v3.3 and
            Glimmer2 v2.13.Intergenic regions not spanned by GeneMark and
            Glimmer2 were blasted against NCBI's non-redundant (NR) database
            and predictions generated based on protein alignments. RNA genes
            were determined usingtRNAscan-SE 1.23 or Rfam v8.0. This sequence
            was finished as follows unless otherwise noted: all regions were
            double stranded, sequenced with an alternate chemistries or covered
            by high quality data (i.e., phred quality >=30); an attempt was
            made to resolve all sequencing problems, such as compressions and
            repeats; all regions were covered by sequence from more than one
            m13 subclone.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Citrobacter koseri ATCC BAA-895"
                     /mol_type="genomic DNA"
                     /strain="ATCC BAA-895"
                     /db_xref="ATCC:BAA-895"
                     /db_xref="taxon:290338"
                     /plasmid="pCKO2"
     gene            3..179
                     /locus_tag="CKO_RS23535"
                     /old_locus_tag="CKO_pCKO2p07156"
                     /db_xref="GeneID:64123168"
     CDS             3..179
                     /locus_tag="CKO_RS23535"
                     /old_locus_tag="CKO_pCKO2p07156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /db_xref="GeneID:64123168"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            169..513
                     /locus_tag="CKO_RS21960"
                     /old_locus_tag="CKO_pCKO2p07157"
                     /db_xref="GeneID:45138599"
     CDS             169..513
                     /locus_tag="CKO_RS21960"
                     /old_locus_tag="CKO_pCKO2p07157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004197764.1"
                     /db_xref="GeneID:45138599"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            722..>1156
                     /locus_tag="CKO_RS23680"
                     /pseudo=""
                     /db_xref="GeneID:71692165"
     CDS             722..>1156
                     /locus_tag="CKO_RS23680"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /db_xref="GeneID:71692165"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1191..1700
                     /locus_tag="CKO_RS23205"
                     /db_xref="GeneID:45138660"
     CDS             1191..1700
                     /locus_tag="CKO_RS23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_024131073.1"
                     /db_xref="GeneID:45138660"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGLSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            1707..1919
                     /locus_tag="CKO_RS23210"
                     /db_xref="GeneID:45138661"
     CDS             1707..1919
                     /locus_tag="CKO_RS23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /db_xref="GeneID:45138661"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2296..2424)
                     /locus_tag="CKO_RS23710"
                     /db_xref="GeneID:80258105"
     CDS             complement(2296..2424)
                     /locus_tag="CKO_RS23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /db_xref="GeneID:80258105"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            2587..2811
                     /locus_tag="CKO_RS22930"
                     /old_locus_tag="CKO_pCKO2p07160"
                     /db_xref="GeneID:45138643"
     CDS             2587..2811
                     /locus_tag="CKO_RS22930"
                     /old_locus_tag="CKO_pCKO2p07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /db_xref="GeneID:45138643"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            2795..3061
                     /locus_tag="CKO_RS21980"
                     /old_locus_tag="CKO_pCKO2p07161"
                     /db_xref="GeneID:45138600"
     CDS             2795..3061
                     /locus_tag="CKO_RS21980"
                     /old_locus_tag="CKO_pCKO2p07161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /db_xref="GeneID:45138600"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3131..3481)
                     /locus_tag="CKO_RS21985"
                     /old_locus_tag="CKO_pCKO2p07163"
                     /db_xref="GeneID:45138601"
     CDS             complement(3131..3481)
                     /locus_tag="CKO_RS21985"
                     /old_locus_tag="CKO_pCKO2p07163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /db_xref="GeneID:45138601"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3547..4092)
                     /locus_tag="CKO_RS21990"
                     /old_locus_tag="CKO_pCKO2p07164"
                     /db_xref="GeneID:45138602"
     CDS             complement(3547..4092)
                     /locus_tag="CKO_RS21990"
                     /old_locus_tag="CKO_pCKO2p07164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197758.1"
                     /db_xref="GeneID:45138602"
                     /translation="MFFKRHLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(4118..4483)
                     /locus_tag="CKO_RS21995"
                     /old_locus_tag="CKO_pCKO2p07165"
                     /db_xref="GeneID:45138603"
     CDS             complement(4118..4483)
                     /locus_tag="CKO_RS21995"
                     /old_locus_tag="CKO_pCKO2p07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226331160.1"
                     /db_xref="GeneID:45138603"
                     /translation="MRNKKRNSEGGGVMRERTNIMLDIGLKQSLQRLSNKTKMSLSDLI
                     NQRLSGSLSVEERIGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINE
                     MMVLKEYFRAKFYDEIK"
                     /name="hypothetical protein"
     gene            complement(4806..4964)
                     /locus_tag="CKO_RS23735"
                     /db_xref="GeneID:89764189"
     CDS             complement(4806..4964)
                     /locus_tag="CKO_RS23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /db_xref="GeneID:89764189"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          5438..5518
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.095"
                     /coverage="98.0"
                     /evalue="6.71e-19"
     Typing          complement(4856..5253)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.801"
                     /coverage="91.0"
                     /evalue="6.52e-141"
     Typing          504..2035
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taatgcagaa gccgaagaag aagccgctcg tcgtaaaaaa aagatcctgg catccggcag
       61 tgatggcaaa tctgatgcgt caggcacagg aggaagatta ccgccgggag cagatggcag
      121 agcttgagcg ttcagcccgg tcacattttg cggatatcgg aggagaggat ggcggttaag
      181 cgtacccgtt ttttaggtat ccgggtgacc gatggggagt accagcagct gctggagcgc
      241 tgcaatggaa ggcaactggc agtgtggatg cgggaaacct gtctcgatac gcgcccggcg
      301 cggtcgttgc ggctgccgtc catcgatccg gttttgctgc gccagcttgc cgggatgggg
      361 aacaacctca accagatcgc ccggaaaatt aacggcggcc agtggtcagg cgctgaccgg
      421 gttcaggtgg tggccgcgct gatggccatc gatgccggac tcgagcggct gcggcatgcc
      481 gtgctggaaa agggggcaga cgatgatcgt taagtttcat ccgcgagggc gaggcggcgg
      541 tgccgggccg gtggattatc tgctggggaa agaccgccag cgcgacgggg ccatcgtcct
      601 gcagggaaag ccggaggagg tccgggagct tatcgacgcc tcgccctacg ccaaaaagta
      661 cacctccggc gtcctgtctt ttgctgaaca ggatttaccg cccggccagc gtgaaaagct
      721 gatggcgagc ttcgagcggg ttctgatgcc cggactcgat aaagaccagt acagcgtgct
      781 gtgggttgaa caccgggaca aggggcggct ggaactgaat tttctggtac cgaacacgga
      841 actgctgacc ggcaggcgtc tccagccgta ctacgaccgg gcagaccgtc cgcgcatcga
      901 tgcgtggcag accgtggtga acgggcgact ggggctgcac gacccgaacg caccggagaa
      961 ccggcgcgcg ctggtgacgc catcttcgtt acccaaaacg aagcaggaag ccgcagaggc
     1021 tattacgcgg ggcttactgg ccctcgcctc gtcaggggag cttaaaacgc gtcaggacgt
     1081 cactgaggcg ctggaaagcg caggttttga ggtcgtgcgc accacaaaga gcagcatcag
     1141 cattgccgac ccggacggag ggcgaaacat ccgacttaag ggagccatct atgaacagtc
     1201 ttttaacgct ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag agtaccggcg
     1261 aaatgctgaa agccgcattc agcgagcacg agaagtctgt cagagcggaa ctgagcgaaa
     1321 gcgggaagag aatcagcgcc gccatccgcg cccacgagca gggtatgagc gcggccatgc
     1381 agtcgaaccg cctgagcgtg atgcgtatgg tcgggcggac atggctgacc atcacgatgg
     1441 tgtcaggact gctgttcgcc agcctgagcg gggtgttgtg gtatcagggg agcctgatag
     1501 cgtcaaatct ggccgaaatc gaccggcaga acgcagcgct gtcgaagctg aacgcgaaga
     1561 cctggggcgt gacgtacctg gaagacagca acggacgttt tctggtgctg ccgaagggga
     1621 cggccgtaga caggacgcag agctggacgg tcgggaacgg gctgtcgaag cagaacgcgc
     1681 tgagactggt gaaggagtaa gtgccgatga ccgagctgga aaaacagttg ctgagcgcat
     1741 tagagcagct acagcaggac tactcgaaaa ggctggacga gtgggagagc gccttcgggg
     1801 aatggcggaa aatgtcaggg cttatgcaac gggagaacgc ggcgctgagc gagcgcgtga
     1861 cgcgcttgag tcagcaggtg gaacgcttga gcgggcaact gcagcgtttg agccggtagt
     1921 gcagcgtcat gagatggccg tggccgcaga acgggcgcac gagcaacggc agcacgaaaa
     1981 ggaactgacg gaagcgcgtt cccggcagcg aagctatgac ggtccgtcgc tgggctgaaa
     2041 tgcaggaaaa cagggttctc atcgggcatt tacgctgaaa ccggatgtca ttcacctggc
     2101 tgcatggcta tgcagccagg taaaatttat ctgtcgcgaa cggggcggtt tatcgggtgg
     2161 tttgttgccg gttttacctg actgccaccc cgttcgcggc gaacccgtcc ggggcggtgc
     2221 gggcaacggg tgttatgttt aatcagcgtg aacggcagat acaaaaaaag ccccggaggg
     2281 cttttaacta ctgacttatt tttttgaaga tttgctgggt ttcaatttgt cagtatttcc
     2341 cggcgttttt ttattgtcac gctggcgttt atcaggttcc cctgttgaat cggcaattcg
     2401 tttcggaccc ggcttaagac ccataatcgt tatcctttca atagttaaga ggaaagccct
     2461 caagctagat atattgaccg cgttacggat tatcaagagg ggctaacaag attatttttt
     2521 gttcttttag gttgattgtg gtactgttgc tatacatgta tagcaaatgg tgttcgggag
     2581 gtttatatgc ttgctatccg gttatctgat gagattgagt cccgtctgga ctcgctggcg
     2641 aagcaaaccg gcagaacaaa gacgttttat gcgcgggaag caatactggc gcatctggaa
     2701 gacctggagg attattatct ttcagcagaa actgctgcac gcgttcgccg tggtgatgaa
     2761 gcagtgcatt cgtctgaaga cgtgaggaag tcacttggtc tggacgatta actattccga
     2821 tcgggcgctc aaatcgttac gcaagatgga caaacagaac gcacgacgga ttgtggattt
     2881 tatgagttta cgcattgcag ttgctgccga tcctcgccag tcagggaagc cgctcaaagg
     2941 tgagctgggc gagttctggc gctatcgggt gggagattat cgcgttctgt gtgagatccg
     3001 agatgacgag cttgttatcc ttgccgccac gattggacat cgccgcgaag tttacgactg
     3061 aagcccgcta tgcgggcttt tttgatgccg acaacaggta ttatggtaaa tactcgacta
     3121 actacgtgtt ctactgtttg tcggttttag ttaaccagca ggttgtctta acgataatgt
     3181 agccgttatt tttaactaat aaggtcatat tgttaacgtc aggcataata aaagacatta
     3241 tggcctcaga gttatcatca gaagcagctc tctgcattac tacttcgatt cccttttctt
     3301 tgaatctata agtatccagt tgcctattaa acttagaaag acctacaacc atacttagtt
     3361 tgttatcttt aacagtaaga gatgcttcct gagtaccatt acatcggtag taagcggtgt
     3421 aaccgtcagc aaaagatcga ccggggaata taaatgctag gaacgcaatt aaccaataca
     3481 tagttgagaa tcttggtatt acacatttca ctatcctctc tttcattttt cgttcaccat
     3541 actttgttat ctgattttta atttttctat tttataaacc aagtccctac caaaaaggcg
     3601 aaaattatcc tcaaataata ataattgttt aagaacatcc atgaaagcag ctgcttcacc
     3661 ctcagttatg ggagctactc tatataacat ataacctgag ttaagggttt cttttatact
     3721 ttcttcaatg ctcattataa atattagtgg attatcattt ttcttccccc atgggtttct
     3781 aattaatcct tcgatgtact caggtccttt gcgtcctatt gcctgcgcaa tattttttat
     3841 ttcatctgcg tgagggcttt gttgtacagc tttgtttatg cctgcttgag tgattttcag
     3901 agactctaca ccgtgtatgt tgtatgagat aaaatgtaat aaattaagat gtaatgcaat
     3961 taatccattt ttgtttacat ctgagtattt ggataaaaaa cgtaatgaag tgttaactct
     4021 cccttgaagc ctaacaattg ggagtgctgc cttgtatatt tccttctccg ttaaatgccg
     4081 tttaaaaaac ataattctcc gcctccgtat agcgtgtcta ttttatttcg tcgtaaaact
     4141 tagctctgaa atattccttt agaaccatca tttcattaat aattgaatct atctcatcca
     4201 ttttttcttg gtattctttt aatggtgaag ggagcggtaa ctcaatattt gaaaagtgat
     4261 tggcgagata gggatcaagg tttctcatcc attcaggctt ttcgccaatg cgttcttcaa
     4321 ctgaaagtga acctgataat ctctggttaa taagatcact tagactcatt ttggttttat
     4381 tcgaaagccg ttgtaaggat tgctttaatc ctatatctag catgatattt gttctttctc
     4441 tcattacccc tcccccctcg ctattgcgtt ttttgttgcg cacaaagcta ccattgcgca
     4501 ttaagaaacg caatgaaaaa ggagggaggg agtggtttgt acatgatgtg cagtgcgctg
     4561 agggcatcag tggcgggtgt ttttccgtgg gatggatgga gctccgggga taacagaact
     4621 gacgatcatt taaaggatct tcttgagatc ctttttttct gcgcgtaatc tcttgcactg
     4681 taaacgaaaa aaccgcctgg ggaggcggtt tgatcgaagg ttaagtcagt tggggaactg
     4741 cttaaccggg taactgggta tacgagaccg cagataccaa aactgttctt tcagtgtagc
     4801 cgcagttagg cacccacttc aagacctctc aatatctctc tcgtatatat ccagttacca
     4861 atggctgctg ccagtggcgt ttttgcgtgt ccgtccgggt tggactcaag atgatagtta
     4921 ccggaaaggg cgcagcagtc gggctgaacg gggggttcgt gcatacagcc cagcttggag
     4981 cgaacgacct aaaccgagcc gagataccag cagcgtgagc tatgagaaag cgccacgctt
     5041 cccgaaaggg aaaaaggcgg aacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5101 gcacgaggga gccgccggag agaaatcacc ggcatcttta agtcctgtcg ggtttcgtcc
     5161 tcctctgact tgagcgtcca tttctgtgat gctcgtcagg ggaggcggag cctatggaaa
     5221 aacggccacg ccgcatcctt ttctccggca ctttctgatt tgagcgcctt tgctttatcc
     5281 cgttatccgt gagccggtca cgcccgccgc agccgaacgg caggagcgca gcgaccgagt
     5341 gagcgaggag gcgtcatttc atctgtccgt gcatggtatt gcggacgctc ctgagccaca
     5401 tatcacacct gccacatgag gcactttcat gaatcgcgct ctcatgtcag catagtaagc
     5461 cagtatacac agcgtaaacg ctgtgactgg ttcagggctg cgccccgaaa cccgctaaca
     5521 ccactgacgc gctggcgctt gtccaacgcc ggagcgaccc ggaaaacatt tccgtgccgt
     5581 ccggcgtttg cgaaggaggg a
//
