LOCUS       NC_009793               9294 bp    DNA     circular CON 22-APR-2024
DEFINITION  Citrobacter koseri ATCC BAA-895 plasmid pCKO3, complete sequence.
ACCESSION   NC_009793
VERSION     NC_009793.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN02603912
            Assembly: GCF_000018045.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter koseri ATCC BAA-895
  ORGANISM  Citrobacter koseri ATCC BAA-895
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   McClelland,M., Sanderson,E.K., Porwollik,S., Spieth,J.,
            Clifton,W.S., Latreille,P., Courtney,L., Wang,C., Pepin,K.,
            Bhonagiri,V., Nash,W., Johnson,M., Thiruvilangam,P. and Wilson,R.
  CONSRTM   The Citrobacter koseri Genome Sequencing Project
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2007) Genetics, Genome Sequencing Center, 4444
            Forest Park Parkway, St. Louis, MO 63108, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000018045.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 01:03:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,418
            CDSs (total)                      :: 4,307
            Genes (coding)                    :: 4,254
            CDSs (with protein)               :: 4,254
            Genes (RNA)                       :: 111
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 53
            CDSs (without protein)            :: 53
            Pseudo Genes (ambiguous residues) :: 0 of 53
            Pseudo Genes (frameshifted)       :: 25 of 53
            Pseudo Genes (incomplete)         :: 26 of 53
            Pseudo Genes (internal stop)      :: 12 of 53
            Pseudo Genes (multiple problems)  :: 9 of 53
            Pseudo Genes (short protein)      :: 1 of 53
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP000823.1.
            Citrobacter (diversus) koseri--Citrobacter cells are isolated from
            water, sewage, soils, and food, as well as from the feces of man
            and other animals, where they may be normal inhabitants. They can
            be found in urine, sputum, and other clinical specimens. They can
            sometimes be opportunistic pathogens particularly in
            immunocompromised patients in hospitals or in infants (Pepperell et
            al., Antimicrob Agents Chemother. 2002 Nov;46(11):3555-60. and
            references therein).
            The strain of Citrobacter koseri being sequenced, strain CDC
            4225-83, was isolated in 1983 in Maryland, where it caused neonatal
            meningitis. It was provided by Caroline Mohr and Melissa Campbell
            of CDC. The strain is available from the American Type Culture
            Collection as ATCC BAA-895 or from the Salmonella Genetic Stock
            Centre as SGSC4696. The genome was sequenced to 8X coverage, using
            plasmid and fosmid libraries and was finished to an error rate of
            less than 1 per 10,000 bases. Automated annotation was performed
            and manual annotation will continue in the labs of Michael
            McClelland and Kenneth Sanderson. The National Institute of Allergy
            and Infectious Diseases (NIAID), National Institutes of Health
            (NIH) has funded this project.
            Coding sequences below are predicted using GeneMark v3.3 and
            Glimmer2 v2.13.Intergenic regions not spanned by GeneMark and
            Glimmer2 were blasted against NCBI's non-redundant (NR) database
            and predictions generated based on protein alignments. RNA genes
            were determined usingtRNAscan-SE 1.23 or Rfam v8.0. This sequence
            was finished as follows unless otherwise noted: all regions were
            double stranded, sequenced with an alternate chemistries or covered
            by high quality data (i.e., phred quality >=30); an attempt was
            made to resolve all sequencing problems, such as compressions and
            repeats; all regions were covered by sequence from more than one
            m13 subclone.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Citrobacter koseri ATCC BAA-895"
                     /mol_type="genomic DNA"
                     /strain="ATCC BAA-895"
                     /db_xref="ATCC:BAA-895"
                     /db_xref="taxon:290338"
                     /plasmid="pCKO3"
     gene            complement(join(8993..9294,1..76))
                     /locus_tag="CKO_RS21905"
                     /db_xref="GeneID:45138588"
     CDS             complement(join(8993..9294,1..76))
                     /locus_tag="CKO_RS21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /db_xref="GeneID:45138588"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            145..324
                     /locus_tag="CKO_RS21910"
                     /old_locus_tag="CKO_pCKO3p06141"
                     /db_xref="GeneID:45138589"
     CDS             145..324
                     /locus_tag="CKO_RS21910"
                     /old_locus_tag="CKO_pCKO3p06141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /db_xref="GeneID:45138589"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(352..882)
                     /locus_tag="CKO_RS21915"
                     /old_locus_tag="CKO_pCKO3p06142"
                     /db_xref="GeneID:45138590"
     CDS             complement(352..882)
                     /locus_tag="CKO_RS21915"
                     /old_locus_tag="CKO_pCKO3p06142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /db_xref="GeneID:45138590"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(889..1620)
                     /locus_tag="CKO_RS21920"
                     /old_locus_tag="CKO_pCKO3p06143"
                     /db_xref="GeneID:45138591"
     CDS             complement(889..1620)
                     /locus_tag="CKO_RS21920"
                     /old_locus_tag="CKO_pCKO3p06143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /db_xref="GeneID:45138591"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(1620..3584)
                     /locus_tag="CKO_RS21925"
                     /old_locus_tag="CKO_pCKO3p06144"
                     /db_xref="GeneID:45138592"
     CDS             complement(1620..3584)
                     /locus_tag="CKO_RS21925"
                     /old_locus_tag="CKO_pCKO3p06144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_012000836.1"
                     /db_xref="GeneID:45138592"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAACLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5065..5214)
                     /locus_tag="CKO_RS21930"
                     /old_locus_tag="CKO_pCKO3p06148"
                     /db_xref="GeneID:45138593"
     CDS             complement(5065..5214)
                     /locus_tag="CKO_RS21930"
                     /old_locus_tag="CKO_pCKO3p06148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /db_xref="GeneID:45138593"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5299..5556)
                     /locus_tag="CKO_RS21935"
                     /old_locus_tag="CKO_pCKO3p06149"
                     /db_xref="GeneID:45138594"
     CDS             complement(5299..5556)
                     /locus_tag="CKO_RS21935"
                     /old_locus_tag="CKO_pCKO3p06149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /db_xref="GeneID:45138594"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(5566..7251)
                     /locus_tag="CKO_RS21940"
                     /old_locus_tag="CKO_pCKO3p06150"
                     /db_xref="GeneID:45138595"
     CDS             complement(5566..7251)
                     /locus_tag="CKO_RS21940"
                     /old_locus_tag="CKO_pCKO3p06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /db_xref="GeneID:45138595"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7654..8073
                     /locus_tag="CKO_RS21945"
                     /old_locus_tag="CKO_pCKO3p06152"
                     /db_xref="GeneID:45138596"
     CDS             7654..8073
                     /locus_tag="CKO_RS21945"
                     /old_locus_tag="CKO_pCKO3p06152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /db_xref="GeneID:45138596"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            8379..8642
                     /locus_tag="CKO_RS21950"
                     /old_locus_tag="CKO_pCKO3p06154"
                     /db_xref="GeneID:45138597"
     CDS             8379..8642
                     /locus_tag="CKO_RS21950"
                     /old_locus_tag="CKO_pCKO3p06154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /db_xref="GeneID:45138597"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            8632..8931
                     /locus_tag="CKO_RS21955"
                     /old_locus_tag="CKO_pCKO3p06155"
                     /db_xref="GeneID:45138598"
     CDS             8632..8931
                     /locus_tag="CKO_RS21955"
                     /old_locus_tag="CKO_pCKO3p06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /db_xref="GeneID:45138598"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     BGC             566..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4384..4962
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(893..1620)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 tcgccatatc ccgatacagg gaacttcggg ctttacccgt cagttccgtc gcctgtctca
       61 ccgtcaccca cgccattgtc tcaccccgct atttttgaga cagtatacct gacaggcgca
      121 caccatgccc ggaaggaaag aatcatgaca aaacaggaaa aaacagccct caatatggct
      181 aaatttatta aggagcagtc gctgctattg ctcgataaac tgaatgagct ggatatggat
      241 accccggccg acatgtgcga gcggctacac gaggatgcgg aaacactctg gcaggtgatg
      301 cgggataagc tcggggagga ataacagcac cggcatggcc ggtgctggca tttatttttt
      361 gccgaacggc caccaccggc gggcaggttt gtcctccggc agttcaggta ccggcacgtt
      421 aaaatagcgg ctgttatatt cccgcatcag ctccgctttc agcttctcgt tcaccgcgct
      481 ttccccgtgc cgctcactca ccacgtcaat catcgcgtta atctgctccg gtgagcactt
      541 cccggcatca accacctgca caccgttctc gatggcgaac cgctccacct tgcgatagag
      601 gtaatcacac agcttcattc ctgtgggttt gttccgttcc cattgcggat ttttaccagt
      661 gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg tcatacatcc cgatggattt
      721 catcatcgcc agatgctggg cccggtcgac aaaacagaaa acatacgccc cggtctgcca
      781 ggcgagcacc accatatctt ccagatgctc gctggtcggc atgatgcagc gctcgccgtt
      841 ccggtcctcc gcgtcttcaa taaataccat gtacagcgcc ataatccctc actccgttgt
      901 cataaattta aagcacgcaa acaaccgttc acgatccagc tctgccgttt tgcgggcctc
      961 gccggtctgt gggttcacga tttcatccac cgacaggatt gcccgctgca ccctgacctg
     1021 cgtcgcccgg tccgggcaga tgtaccagat ttcatcgatg ccgttagctt tgcggttgaa
     1081 cagatacccg gacaccactt cctgataccg gcgacggctc ttgacggtgc gctccacctc
     1141 aaacgccacc gttttaccgt ccagctccgc cagggcgtcc ggcactttca tgcctttaag
     1201 actcagacgg tgcagaatgc gccagccggt ggcccctctg gcttccatat caaggcgcag
     1261 ggattgcacg gccagccggt gcggcagatg tgctgccgac acccttgacg gctgaaacgg
     1321 aataacatcc gccagcggtt cgtcctcgtc gaacgagaac gccacgccat gcggcgtcag
     1381 cccccacacg ctgacctgac ggccgtccac cggatatttg gtccggacca gcagcccttt
     1441 cgccaccagg ctgtcgagga agcggtagac cggctgcacc gccgcaatcc ccagccgccg
     1501 cgcaaccagc tccgccgtgg aacacagctc ttcccgcaac cagcgcaggg tcagccgggt
     1561 tttctccgcg ttccgcgcct gtcgtttcgc gtggctgacg ttgtacagct ccagcgccat
     1621 cagtaaatcc ccgcaaaatc atcatccgct ttcggcccgc cgccgtcggc cgggggccgg
     1681 aactccgcca ggggatcggt tttcgccagc accgggaaac gctgcaacgg gatttcccgg
     1741 cgctcaaccc gcagcggact gctgaacgcc atccgggcca cacccagccc ggtcatgacc
     1801 gcgaacccgt ccggcaggtg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc
     1861 tggatgaagc tgtccccgct gatatgctcc acgttccccg cctcccggct ggcacggcgg
     1921 cgctccacgt ccaccctgat ttccccggtc tgccccgccg cccactgcgc ggactccagg
     1981 caggccgccc ggtaaaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaat
     2041 tcagcgggca gatcctgccc gcactgcccg aaatcgccca gcgactggtg cgcgagcaac
     2101 agattgcagt cccggctgcg cagcgtcccc agtgcgttca gtacatactt cgacagcaga
     2161 tatttaattt catcgagcat cacgctgacg tgtggccact cccggaactc gtcccgcgca
     2221 atgatgattt gcgcgcagcg ggcaaacagc attttttgca cacggatcac cgcctcgtcg
     2281 tccatcgagc cgataacgta caggcagccg ccgccgttga taatccccgc cacatcgatg
     2341 ccactgtccg tctgcaggac gctcaggtca gcgacgtttt ccagcctagt gatcaacccc
     2401 ttcacgtcct ttttcagggc ctccggcagc gcgtacgccg ccgccagcac ctgctgaatg
     2461 tttgccccct gcggaaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg
     2521 tagtggtccg ccacgtcgcc ggtctcgccc aggttaagcc cggcaatcag caggtttttc
     2581 agcgcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga
     2641 tccagcaggg tgaagtccgg acagtgcgcc cggaagacgt gcggcgccca tgcgtcccct
     2701 ttggggtcga agtacaccac cgcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg
     2761 gtggccatca cgctcttgcc gctcccgggc aggccgagga tctgaatgtt ggtcttgcgc
     2821 caggtatcac gcggaaccag caccggcttg ccctgctcat ccttacccag aaacaccgcc
     2881 tgctcctgcc gggcctgctc cgaggaaaaa tcggccagcg cattaaccgg taccggctcc
     2941 ccgaaatgca ggtggcgcac gtcggtcatc tctcctgtcc gctggctgct gcgccggctc
     3001 tcctgattca gtttcgcgtt caccgcctcg tcagcagccg caaaatgacc gtcaagcagc
     3061 aggaacgcca gcagccgcgc caacacaatc ccgacagcca gcccgcccgc gcaccaggca
     3121 atcccgaaca ggttagccgg ctgcagcagg taataatctg cgtttgcgta gaggtccgga
     3181 cgcaggacgg tttctcccca gctctcgata aacacggcga caaccagccc ccccagaagg
     3241 gccagaccgg ccgcgccggt cagccagtac gcgtagttcc agacatgtcg acgtgccctg
     3301 accatccgcc cgggcgaatc cagcgaggcc agcaccgaaa atggcgctgg ccgtttatta
     3361 tcgcgcttat agcgatcggc atactcccgt cgctccttat gactcagccc cacaaaacgc
     3421 atccgtccgg cccaaagttc agccagaccg aaaaacaggc cgataactag gaaagccccg
     3481 gccagaccgt aatactcctg gtacagccag ctgacccacg gataaatctg ccgggccagc
     3541 tccggaatgt gccacttcca ggcaagcgaa tccgtattaa acatgttctc tctccttcag
     3601 acgatggttt gccggcgtga tgtgcagccc ccgctgcaca agccgcggac atgccccaag
     3661 gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag ctttcaccac ggaggccagc
     3721 ctctttccgc cccatgcgga aaaggcgcag ccccggagga acaacgagca cgccccagcg
     3781 tgtgagagga aaaggcggca ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga
     3841 gttcgccata gcgagcggct gaccacccgc aaaatcagga gcagctttgc tgcttcgcca
     3901 ggtgcgtcca gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac gggggccggg
     3961 tgaatttctg gcgtggccgg cgtccagccg gacggagcca gaaagagggg gagagaccgt
     4021 gcgtcggtgg cgtccagcca gcgtaagcag gggatatccc cctgaggtca gccacgccct
     4081 cgcggggatg acctgtattt ctccgtaagg agaaaaaagc aacagagata ggttaacgta
     4141 aaatgttcag cgaaaaaccg atatgattca cctatcctgg taatttatta tcagggaata
     4201 taaagactta atcaggggac aatatgccac atcagcgcgt ccggtgtccg tcttgtaggt
     4261 gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg ctccggccgt gagactgcgg
     4321 cgggcgctgc ggacacatgc aaagttaccc acagattccg tgaataagca ggggactaac
     4381 atgtgaggca aaacggcaag gccacgcagg tggcgttttt ccataggctc cgccctcctg
     4441 actgagttca cataaacaga cgctttttcg gtgcatctgt gggagccggt ggctcaacca
     4501 tgaatcagtc aggagtggcg aaacccgaca ggacttaaag atcatcaccg tttccgatga
     4561 atcgctccct cttgcgctct cctgttccaa ccctgccgtt tcccggatac ctgttccgcc
     4621 tttctccctt gcgggaagtg tggcgctttc tcatagctca cactctggta tctcggctcg
     4681 gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac cccccgttca gcccgactgc
     4741 tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgc aaaacgccac
     4801 tggcagcagc cattggtaac tgggagttcg cagaggattt gtttagctaa acacgcggtt
     4861 gctcttgaag tgtgcgccaa agtccggcta cactggaagg acagatttgg ttgctgtgct
     4921 ctgcgaaagc cagttaccac ggttaagcag ttccccaact gacttaacct tcgatcaaac
     4981 cacctcccca ggtggttttt tcgtttacag ggcaaaagat tacgcgcaga aaaaaaggat
     5041 ctcaagaaga tcctttgatc ttttctactg aaccgcgatc cccgtcagtt cagaggagga
     5101 cgatggtgcc accgttccac cctgaacatc ccggatatag tttgcctgac atgcgaccag
     5161 aagaaagcct gaaaaaatca atataaaaag aaaaacaacc tttacctttt tcatatacct
     5221 tccctgtatt taaaaacccc ggtattcatc cctgaacacc ggaagtctgt atttcaaatc
     5281 atccttgata acttttgctt accagttacc atcccggtaa acaaaggata caaagtaatc
     5341 aaatttacta atatcgatta catttttaaa gtgtggctgt aatatcgaaa cccatgattt
     5401 ttcaacatca aaccaaccat tattaatatt atcctttaaa ggcatcccca gactttcaat
     5461 gacagaacca tcatcaccga agtcttttga gtattcacca cctttaaact cttcggtttt
     5521 cttatcaaac caatgaatat gtaatttaag ccccatattt acctcttaaa gatatttttt
     5581 aatgtttcgt tttggatccg gccctttaac ctgcttaccc gtttttggat cgaatgcccc
     5641 gaggtgttcg ccatcactgg cgcggtaccc ttcaagctca ccgtgctggg aatcccattc
     5701 ataaatttta cgctttttat caccatacca tcgggcccgc ttaccaccac caccttgttt
     5761 aggggtttta ggtttaccct ctttcaactc tcccaacccc ttaatatctt cagtcttggg
     5821 atcaggaaaa taatcatggc cgtaatcttt agttccctta cgaggttttt tcttttcctc
     5881 atttaattta ttttctgcat ccttcgcttt tttctccttt tgtttccggc tctccatcgc
     5941 cccggaaagc gcagccttat ccctggataa ggattcattc atttcgttta aagatgtctg
     6001 ggcagctttt aatttctcat tctgattctg aatatcctta tcaagatagc gaacctgatc
     6061 ctgccacccg gagccatatt catgaggcgg gttataagtc acaaaatcat ctttatttgc
     6121 ctctgcctct tttacctttt tatcggcttc ctgaacagca gcccttgcac cggggatcgc
     6181 cttctctgtc gatgcaatgc gactgtttaa ggtcgcaata tttttatctt cagcatccag
     6241 ctcagctttc gctttatcat actctctttt cagcccctct tccgggtgag cagcgtccca
     6301 tgcctgctgg cgacgctttt cttcctccag acgctgtttt acctgtgccg gagtaagaac
     6361 atccgtcacc gacacataga ccggtttctg tccgctctct ttcgggaaac gaataacggc
     6421 ctcacgggag ttaccaccgg ccgtaaaacc agccggacgt gaatcacctt tttcagcaat
     6481 aatgcctttt ggcggggctt tcgcggccgg aacaccttta ggtacgctca cctgcagaga
     6541 cgggagaccc ggaatcgaca cactgaatac ccccggacgt tttgtcggtt tcgcatccac
     6601 cacaggaaca ctcattggcc ggccggtgac aaccgcaata tgctgccgct cgtccttcac
     6661 aatatccgcc acacgtttag tgacgctgac cgtcgcctgg tccagcggca gggagctgac
     6721 cggcgtctcc gttaccgtgt ctgccggcaa tgacgtcacg atttttgaca tcattttcgg
     6781 atcatctttt gctatctcag aaggcagcac tccgtaaatc gcaatccccc acagaccaaa
     6841 cttaaacgga cctttcaggg cagccagcac atcagcaaca gcggccgaca acgcatcgcc
     6901 ggaaacggat aaagccagtc cttcagcccc cggagtggcc agggcaggca gaccgaaggc
     6961 catcgcggtg gccgaagact gtccgccacc agagctcccg ctatgattgc cgctgttacc
     7021 gccattacca ccgggtttac tcccgccgtt accaaactcc cccgggttat agttatgaat
     7081 atccccgtta ggtgtatgcg tcgtacccca gcccggcccg ctgtttggat ccgtaccacc
     7141 acctgatgaa gggccacctt tacctgatgt accgttcaca ttcccactgg cctgaccacc
     7201 attgtgtccc agacctgaat tacccggacc tcgtccgtct ccgccactca tatacttttc
     7261 ctctttgact tttaaaacaa taaactaaaa taaaatactg tacatataac cactggtttt
     7321 atatacagta taaaaattac gctgccttgt ttttcatgtc aagtctgtaa atttgcgttt
     7381 ttgtgaaaat gatcaaaaaa acagccactc acaccatgcg caatcccctc cagatgcaac
     7441 aaaaaccagc acaaacggcg ccgttctccg gcctcagtat gttttactga aatcattata
     7501 tgagatacca gaaagccccc tccgggacag gaaaagaaac ggggggtctg gttcgggttg
     7561 ttcggtcact gaacacggtc agaaactgat gataagatag cctgcggata tataactcaa
     7621 tgagaaaaaa gaattaaagg taaggagacg ctcatgagac gttccggcag caaactgtgt
     7681 cttatcgcca tgataacagc gacagcaact ctcactgcct gtgctccaaa gggcagcgtg
     7741 gaacagcata cccggcatta cgtatatgct tcagatgacg gttttgatcc caatttttcc
     7801 acccagaaag ctgacacaac acgaatgatg gtgccttttt tccggcagtt ctgggatatg
     7861 ggcgcaaagg ataaagcgac cggaaaatca cggagtgatg tgcagcaacg cattcagcag
     7921 tttcacagtc aggaattttt aaactcactc caaggcacaa cacaatttgc gggtaccgat
     7981 taccgcagca aagacctcac accgaaaaaa tcaaggctac tggctgacac catttcagct
     8041 gtttatctcg atggctatga aggcagacag taagtggatt taccataacg cgtcattacg
     8101 cgcaccgctc agacgggctc agggcgtttc cggcggctac gggataaaac aggcaacaac
     8161 ccccccggaa aactgccgga aacgcgcccg ataaatttta tcctaatgca tgcctatgca
     8221 cgcaactgca tggcatatgt tttgacgtaa aatggatgcc gtaagccctt ttttaccgac
     8281 tttctgcttc atcagcctga ctgacacgac catattcttt cagaccgccc cctgctaaac
     8341 ttccttcata ttgccgcatt aacccgggag gctccgcaat gagcaacact acacactacg
     8401 aaaacgccaa ctttcttcgg gaactggctg aaagcctgcc ccgcatactg ccggaaggtg
     8461 gcccggacaa agctgcgctg ttgcagcgac tggctaacga agaactggcc caggcagagt
     8521 atgaagatca ggttcgcgcc aaagtgaccg ccgcccgtgc tgacacgcgt ccgggtatga
     8581 ccacggaaca actccgccag cgcctgcatg gccgttatca ggaactgcgc gatgccgtat
     8641 gacgtggaat ggaagcaggg agccatcgag gatgtaacga cgctgtttga ctatatcgcg
     8701 gaaaattcat ccctgtggga tgcgcagaac gttaccgacc gggttctcgc tgcggcggac
     8761 aaactggccg acttcccgcg actctatgaa gtggactcac gctatggcga ggacgttcgc
     8821 cggatcagtc tgatgggaca gcatgtcctc tatgatgttg atgaccagac cagaaaagtc
     8881 cgggttctgg ccgtggtcgg ccagcgccag aatccccata ttgttctcta ataaccggtg
     8941 acggggacgc aattcattgc gtggccgtcg ccggttggac aagcacagcg cgtcagcgtt
     9001 ttccccacgg ccaccaccgg cgcggtgttc cggccggggt ctctgctgtc tccggtttgt
     9061 gttccagcag tctcatggac tgcatttcct gccgtagccc ggccacctcc tccctgagtg
     9121 cttttatctc cgcgatcaac gcgtcggagg ccattttttc agtctcaccc gacagtctca
     9181 actcgtcccg ctcgtgagtc tcatcctgtc ttaactcacc gtacacccgg ataagctcgc
     9241 tggtatccac cacccggccg ccatcagctt ccgtacggta tgatacgcgg cctt
//
