LOCUS       NC_008597               6222 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pVI678, complete sequence.
ACCESSION   NC_008597
VERSION     NC_008597.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225718
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Slettemeas,J.S. and Sunde,M.
  TITLE     Resistance plasmid from wild animals
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Slettemeas,J.S. and Sunde,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Section of Bacteriology, National Veterinary
            Institute, Ullevalsveien 68, Oslo 0454, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009951395.2-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 02:53:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            EF090911.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /isolate="2001-10-678"
                     /host="Cervus elaphus (wild-living)"
                     /db_xref="taxon:562"
                     /plasmid="pVI678"
     gene            141..956
                     /gene="sul2"
                     /locus_tag="HS439_RS00005"
                     /old_locus_tag="pVI678_p1"
     ARG             142..953
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1017..1820
                     /gene="aph(3'')-Ib"
                     /locus_tag="HS439_RS00010"
                     /old_locus_tag="pVI678_p2"
     ARG             1018..1817
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1820..2656
                     /locus_tag="HS439_RS00015"
                     /old_locus_tag="pVI678_p3"
     CDS             1820..2656
                     /locus_tag="HS439_RS00015"
                     /old_locus_tag="pVI678_p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<2763..3167)
                     /locus_tag="HS439_RS00020"
                     /old_locus_tag="pVI678_p4"
                     /pseudo=""
     CDS             complement(<2763..3167)
                     /locus_tag="HS439_RS00020"
                     /old_locus_tag="pVI678_p4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(3211..3633)
                     /locus_tag="HS439_RS00025"
                     /old_locus_tag="pVI678_p5"
     CDS             complement(3211..3633)
                     /locus_tag="HS439_RS00025"
                     /old_locus_tag="pVI678_p5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3818..4258
                     /locus_tag="HS439_RS00030"
                     /old_locus_tag="pVI678_p6"
     CDS             3818..4258
                     /locus_tag="HS439_RS00030"
                     /old_locus_tag="pVI678_p6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(5025..5174)
                     /locus_tag="HS439_RS00035"
                     /old_locus_tag="pVI678_p8"
     CDS             complement(5025..5174)
                     /locus_tag="HS439_RS00035"
                     /old_locus_tag="pVI678_p8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5745..5903
                     /locus_tag="HS439_RS00040"
     CDS             5745..5903
                     /locus_tag="HS439_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             1821..2653
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(5210..5383)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          5307..5912
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gatatattta tttccaaaat gctacagata catcatcatg aaacggatca ccgcaaacag
       61 gttactcgcc tggggattcc ctttcgaccc gagcatccgt atgagactca tgctcgatta
      121 ttattattat agaagccccc atgaataaat cgctcatcat tttcggcatc gtcaacataa
      181 cctcggacag tttctccgat ggaggccggt atctggcgcc agacgcagcc attgcgcagg
      241 cgcgtaagct gatggccgag ggggcagatg tgatcgacct cggtccggca tccagcaatc
      301 ccgacgccgc gcctgtttcg tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg
      361 cgctcaaggc agatggcatt cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct
      421 atgccttgtc gcgtggtgtg gcctatctca atgatattcg cggttttcca gacgctgcgt
      481 tctatccgca attggcgaaa tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg
      541 ggcaggcaga tcggcgcgag gcacccgctg gcgacatcat ggatcacatt gcggcgttct
      601 ttgacgcgcg catcgcggcg ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg
      661 atcccggcat gggttttttt ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc
      721 ggttcgatga attgcggctg cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat
      781 cctttctgcg cgcgctcaca ggccgtggtc cgggggatgt cggggccgcg acactcgctg
      841 cagagcttgc cgccgccgca ggtggagctg acttcatccg cacacacgag ccgcgcccct
      901 tgcgcgacgg gctggcggta ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc
      961 acattcggga tatttctcta tattcgcgct tcatcagaaa actgaaggaa cctccattga
     1021 atcgaactaa tatttttttt ggtgaatcgc attctgactg gttgcctgtc agaggcggag
     1081 aatctggtga ttttgttttt cgacgtggtg acgggcatgc cttcgcgaaa atcgcacctg
     1141 cttcccgccg cggtgagctc gctggagagc gtgaccgcct catttggctc aaaggtcgag
     1201 gtgtggcttg ccccgaggtc atcaactggc aggaggaaca ggagggtgca tgcttggtga
     1261 taacggcaat tccgggagta ccggcggctg atctgtctgg agcggatttg ctcaaagcgt
     1321 ggccgtcaat ggggcagcaa cttggcgctg ttcacagcct atcggttgat caatgtccgt
     1381 ttgagcgcag gctgtcgcga atgttcggac gcgccgttga tgtggtgtcc cgcaatgccg
     1441 tcaatcccga cttcttaccg gacgaggaca agagtacgcc gcagctcgat cttttggctc
     1501 gtgtcgaacg agagctaccg gtgcggctcg accaagagcg caccgatatg gttgtttgcc
     1561 atggtgatcc ctgcatgccg aacttcatgg tggaccctaa aactcttcaa tgcacgggtc
     1621 tgatcgacct tgggcggctc ggaacagcag atcgctatgc cgatttggca ctcatgattg
     1681 ctaacgccga agagaactgg gcagcgccag atgaagcaga gcgcgccttc gctgtcctat
     1741 tcaatgtatt ggggatcgaa gcccccgacc gcgaacgcct tgccttctat ctgcgattgg
     1801 accctctgac ttggggttga tgttcatgcc gcctgttttt cctgctcatt ggcacgtttc
     1861 gcaacctgtt ctcattgcgg acaccttttc cagcctcgtt tggaaagttt cattgccaga
     1921 cgggactcct gcaatcgtca agggattgaa acctatagaa gacattgctg atgaactgcg
     1981 cggggccgac tatctggtat ggcgcaatgg gaggggagca gtccggttgc tcggtcgtga
     2041 gaacaatctg atgttgctcg aatatgccgg ggagcgaatg ctctctcaca tcgttgccga
     2101 gcacggcgac taccaggcga ccgaaattgc agcggaacta atggcgaagc tgtatgccgc
     2161 atctgaggaa cccctgcctt ctgcccttct cccgatccgg gatcgctttg cagctttgtt
     2221 tcagcgggcg cgcgatgatc aaaacgcagg ttgtcaaact gactacgtcc acgcggcgat
     2281 tatagccgat caaatgatga gcaatgcctc ggaactgcgt gggctacatg gcgatctgca
     2341 tcatgaaaac atcatgttct ccagtcgcgg ctggctggtg atagatcccg tcggtctggt
     2401 cggtgaagtg ggctttggcg ccgccaatat gttctacgat ccggctgaca gagacgacct
     2461 ttgtctcgat cctagacgca ttgcacagat ggcggacgca ttctctcgtg cgctggacgt
     2521 cgatccgcgt cgcctgctcg accaggcgta cgcttatggg tgcctttccg cagcttggaa
     2581 cgcggatgga gaagaggagc aacgcgatct agctatcgcg gccgcgatca agcaggtgcg
     2641 acagacgtca tactagatat caagcgactt ctcctatccc ctgggaacac atcaatctca
     2701 ccggagaata tcgctggcca aagccttagc gtaggattcc gccccttccc gcaaacgacc
     2761 ccaaacagga aacgcagctg aaacgggaag ctcaacaccc actgacgcat gggttgttca
     2821 ggcagtactt catcaaccag caaggcggca ctttcggcca tccgccgcgc cccacagctc
     2881 gggcagaaac cgcgacgctt acagctgaaa gcgaccaggt gctcggcgtg gcaagactcg
     2941 cagcgaaccc gtagaaagcc atgctccagc cgcccgcatt ggagaaattc ttcaaattcc
     3001 cgttgcacat agcccggcaa ttcctttccc tgctctgcca taagcgcagc gaatgccggg
     3061 taatactcgt caacgatctg atagagaagg gtttgctcgg gtcggtggct ctggtaacga
     3121 ccagtatccc gatcccggct ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt
     3181 gcaatactgt gtttacatac agtctatcgc ttagcggaaa gttcttttac cctcagccga
     3241 aatgcctgcc gttgctagac attgccagcc agtgcccgtc actcccactc ttacattgtt
     3301 cgcagtatct aggtacatgt tattgcgtgc accttcatga tgtataatgt tattaatgac
     3361 agcagaaaga tcgacagaat taatattctt cgtacctggg acagttatcg ggtgtcggcc
     3421 agtgatgaga ccaaaggatg cagtataaga tgaaccacta ctattatcat ggccaccatt
     3481 accaccaccg ttaaaactac ctcccgaact tccagagccg ctatgaccat cattgtaggg
     3541 gtcatttggt cccatgtgac caaaaccacc gacagtaccg ctatttaact ctgtactcat
     3601 aaaattatcc tctttgactt ttaaaaacag caaaaactgt atttataaca atgtgtttat
     3661 atacagtatt cgaaatacta cgcccgtcaa aaccacaccg tcaacaggtt ttttgtgtat
     3721 ttttcacgcc tcacgggcag cctcagacct tttgtgttaa aacagtaata tgttctgctg
     3781 ttacaatacg gtacacacag gagacactgt cacttttatg gcatggcaca cagttcaaga
     3841 gacactgaaa ttaacaggaa agtcgcgctc gcaattatat cgtgacatgg ctaaaggcct
     3901 tgtcagctat cgcacaggaa aaaatgatcg ccgggagttc gagaccagtg aactgattcg
     3961 ggcctatggc gagctgcgca cttctgagac accaaaaagg cacaaggagg gacactccaa
     4021 atctgtcgtt agccaggcgg aaaatgctca gctagacgtc attcagcaga aactggacgc
     4081 tctgcagcag actgttaccc taatgcttga agacaagatg gcgcgggaag cagaaaaccg
     4141 cctgcgaaat gaggagaggg aacaactaca ggccgaaatt agccgtctgg ctgacgcgct
     4201 ggcacaggaa aaaaagcggg gattctggtc gcggctgttt cgctccggtt cagagtaaca
     4261 gtgcaggtga ctctgattta ccataacatc tgttatcagc accgcctgag aggccctcag
     4321 ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa
     4381 cgcgcaggct ctgtttttac ggcatgcatg agtatgcgca caagtgcata gcgcggaaat
     4441 tcacaccatt cagccggggt aagtcctgtt tccggcgttc ttcccatccg aaaacggtga
     4501 acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg aaaacccgct
     4561 gtcagcacgt tcaggaaggg ggtgatgtac aaatggcaca gaggatgaaa gtgacgtatg
     4621 agctgaagga atgatgtaaa agtgatgtat aagtgatgta aaagtgatgt ataagtgatg
     4681 taaaagtgat gtatgaactg aaggagcgat gtaattctgt agtcaggaag aataacccgt
     4741 tcatgcaggc atgaacgata cagtgaggaa cgcttttttt gtcacgatct ccccttaacc
     4801 tgcgtaaatc acccgccatg gttgattatt tttcaacctc aaaatggtca tacacctaac
     4861 gcagccgcga ttttcatcgc aactgcatga attaaaagaa aaaaaaggaa tatttcgttt
     4921 tcacgagtaa tacccgggta ttaaaaatcc actgtaatta catgaaaata aagaaataaa
     4981 aggtaatacc ggagtattac cacgtacgcg ggcaagcgaa aaagctataa cgcttcccgg
     5041 ccgacaggcg acccaaaatc cacaggggca tgcaggttct cttcagtaac ctgcgccatt
     5101 aaatcaaaaa ggtcatattc gccgggaagc tgcgtatcag tcgaatccag ttgttcatca
     5161 tattcagatg gcatggggtg atatccagta gctgagaggt tgttagcggg tttcggggcg
     5221 cagccctgaa ccagtcatgt agcgctagcg gagtgtatac tggcttacta tgttgccacg
     5281 attgcgaaaa tcagtgaagt gcttcatgtg gcaggagaaa aaaggcagca ccggcgcgtc
     5341 agcagaatat gcgatacagg agatattccg cttcctcgct cactgactcg ctacgctcgg
     5401 tcgttcggct gcggcgagcg gtaccggctt acggacgggg acggaaattt cctggaagat
     5461 accaggaaga cacttagcaa agaagagata aggccacggc gaagccgttt ttccataggc
     5521 tccgcccccc tgacaagcat cgcaaaatct gacgctcaaa tcagtggtgg cgaaacccga
     5581 caggactata aagatcccag gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc
     5641 ctgcctttcg gtttaccggt gtcattccgc tgttatggcc gcggtttctc attccacgcc
     5701 tgacactcag ttccgtgaag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg
     5761 ttcagtccga ccgctgcgcc ttgtccggta actatcaact tgagtccaac ccggaaagac
     5821 acgacaaaac gccactggca gcagccatta gtaacaggag tgcacgagaa gatagattaa
     5881 gaggtcttga agtagaggcc tgactgcggc tacactgaaa gaacagtttt ggtaatggcg
     5941 gtctcgtgaa ccctgttacc gggttaagca gttccccaac tgacttaacc ttcgaacaaa
     6001 ccgcctcccc aggcggtttt ttcgtttaca gagcaagaga ttacgattca ttgaaaagga
     6061 tctcaagaag atcctttgta gattactatc aattaatctt ccgaacatga caccagtcac
     6121 tgttgattac ggttaaaccg ctccgtaaca ggtttccctc gtattggtga cttgcagcac
     6181 caaagaagca aaaaagataa atatatctac ttattgagag at
//
