LOCUS       NC_008490               8985 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pMG828-5, complete sequence.
ACCESSION   NC_008490
VERSION     NC_008490.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225668
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Shu,H.-Y. and Lin,G.-H.
  TITLE     Comparative analysis of five indigenous plasmids from a clinical
            strain of Escherichia coli with biofilm formation ability
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8985)
  AUTHORS   Shu,H.-Y. and Lin,G.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2006) Sequencing Core, Genome Research Center,
            National Yang Ming University, 155 Li-Nong St., Sec. 2, Peitou,
            Taipei 112, Taiwan
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009951315.2-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 02:53:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 8
            CDSs (with protein)               :: 8
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            DQ995355.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MG828"
                     /db_xref="taxon:562"
                     /plasmid="pMG828-5"
                     /note="clinical isolate"
     gene            join(8666..8985,1..1174)
                     /locus_tag="HS447_RS00005"
     CDS             join(8666..8985,1..1174)
                     /locus_tag="HS447_RS00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WP_001120888.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            complement(<1410..1652)
                     /gene="glmM"
                     /locus_tag="HS447_RS00015"
                     /pseudo=""
     CDS             complement(<1410..1652)
                     /gene="glmM"
                     /locus_tag="HS447_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(1739..2554)
                     /gene="sul2"
                     /locus_tag="HS447_RS00020"
                     /old_locus_tag="pMG8285_02"
     ARG             complement(1743..2554)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            <2717..2839
                     /gene="sugE"
                     /locus_tag="HS447_RS00025"
                     /pseudo=""
     CDS             <2717..2839
                     /gene="sugE"
                     /locus_tag="HS447_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /name="sugE"
     gene            complement(2836..3369)
                     /gene="blc"
                     /locus_tag="HS447_RS00030"
                     /old_locus_tag="pMG8285_03"
     CDS             complement(2836..3369)
                     /gene="blc"
                     /locus_tag="HS447_RS00030"
                     /old_locus_tag="pMG8285_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="WP_011666806.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWYE
                     IARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRAAL
                     KVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVATREG
                     FDISKLIWVQQPGS"
                     /name="blc"
     gene            complement(3458..4600)
                     /gene="ampC"
                     /locus_tag="HS447_RS00035"
                     /old_locus_tag="pMG8285_04"
     CDS             complement(3458..4600)
                     /gene="ampC"
                     /locus_tag="HS447_RS00035"
                     /old_locus_tag="pMG8285_04"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /GO_component="GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /GO_function="GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMY2/MIR/ACT/EC family class C beta-lactamase"
                     /protein_id="WP_011666807.1"
                     /translation="MLKTTLCALLITASCSTLAASFSEQQINDIVHRTITPLVEQQKIP
                     GIAVAVIYQGKPYYFTWGYADVTQKRPVTQQTLFELGSVSKTFTGVLSGDAIARGEINL
                     SDHVTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSATDLLRFYQNWQPEWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLTHTWINVPPAEEKNYAWGY
                     REGKAVHVSPGMLDAEAYGVKSTIEDMARWVRSNINPRDINDKTLQQGIQLAQSRYWQT
                     GDMYQGLGWEMLDWPVDPDSIINGSDSKIALAARPVKAMMPPTPAVRSSWVHKTGATGG
                     FGSYIAFIPEKELGIVMLANKNYPNPARVTAAWQILNALQ"
                     /name="ampC"
     gene            4732..>5649
                     /locus_tag="HS447_RS00040"
                     /pseudo=""
     CDS             4732..>5649
                     /locus_tag="HS447_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /name="IS4-like element ISVsa5 family transposase"
     gene            complement(5952..6110)
                     /locus_tag="HS447_RS00060"
     CDS             complement(5952..6110)
                     /locus_tag="HS447_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            6681..6830
                     /locus_tag="HS447_RS00045"
     CDS             6681..6830
                     /locus_tag="HS447_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            complement(7575..8015)
                     /locus_tag="HS447_RS00050"
                     /old_locus_tag="pMG8285_05"
     CDS             complement(7575..8015)
                     /locus_tag="HS447_RS00050"
                     /old_locus_tag="pMG8285_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            8200..8622
                     /locus_tag="HS447_RS00055"
     CDS             8200..8622
                     /locus_tag="HS447_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     ARG             complement(3459..4600)
                     /name="Escherichia coli ampC beta-lactamase"
                     /gene="Escherichia coli ampC beta-lactamase"
                     /gene_name="ampC-type beta-lactamase"
                     /tool="rgi"
                     /drug_class="cephalosporin; penam"
                     /identity="91.32"
                     /coverage="100.8"
     Typing          6473..6646
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          complement(5944..6549)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgccttgctg gttgatgaag tactgcctga acaacccatg cgtcagtggg tgttgagctt
       61 cccgtttcag ctgcgtttcc tgtttgccag ccggcccgag atcatggggt gggtgctggg
      121 catcgtttac cgcgtcattg ccacgcacct ggtcaagaaa gcgggccata cccaccaagt
      181 ggccaagacg ggcgcggtca ccctgatcca gcgttttgga tcggcgctca atctgaatgt
      241 tcacttccac atgctgtttc tcgacggtgt gtatgtcgag caatcccacg gctcagcgcg
      301 tttccgctgg gtcaaggcgc cgaccagccc agagctcacc cagctgacgc acaccatcgc
      361 ccaccgggtg ggtcgctatc tggaacggca aggcctgctg gaacgggatg tcgaaaacag
      421 ctatctggcc tcggatgcgg tggatgacga cccgatgaca cccctgctgg ggcactcgat
      481 cacttaccgt atcgctgtcg gttcacaggc ggggcgaaag gtgttcactt tgcaaactct
      541 gccgaccagt ggtgatccgt tcggtgacgg gattggcaag gtagccgggt ccagcctgca
      601 cgccggcgtg gcggccaggg ccgatgaacg caagaagctc gaacggctgt gccggtacat
      661 cagccgcccg gcggtatccg agaagcggct gtcgttaaca cgaggcggca acgtgcgcta
      721 ccagctcaag acgccgtacc gggacggcac cacgcacgtc attttcgaac cattggattt
      781 cattgcaagg ctggccgccc tggtaccgaa gcccagagtc aacctaaccc gcttccacgg
      841 ggtgttcgca cccaacagtc ggcaccgggc gttggtcacg ccggcaaaac ggggcagggg
      901 caacaaggtc agggtggctg atgaaccggc aacaccagca caacggcgag cgtcgatgac
      961 atgggcgcaa cggctcaagc gtgttttcaa tatcgacatc gagacctgca gcggctgcgg
     1021 cggcgccatg aaagtcatcg cctgcattga agaccctata gtgatcaagc agatccttga
     1081 tcacctgaag cacaaagccg aaaccagcgg gaccagggcg ttacccgaaa gccgggcgcc
     1141 accggctgag ctgctcctgg gtctgtttga ctgacgagcc tgaaggccaa cgataccaat
     1201 caaaatgctg cgttcacagc gccgcggcag ggatccgccg tgctggttgt cggaaaagga
     1261 gccgctagtg ggaaagagga gggtaaattt tcagcgttgc tggctccccg tcagccggat
     1321 tgggttgcat cgcaggggtg tcgaaagagt caactgtggt ccaaagctgt tggacttggg
     1381 tgaaaagggc gtttattctt cctatacgtt gtcggcagcg ggccaaaaag gaatacgtcc
     1441 atgcccatcg aggtgaaacc ggctgtgagc gcgggttcaa gcatatagcc cgacaggcgc
     1501 gtatccttgc cgatcacgac acgatggcgg tggtcaccgc gacgaaagac acggccagcc
     1561 gccatgccga cgcgcaaggc ggtttccgcc gtcatcgcgc cttcgttggc tttgccacga
     1621 ataccgtctg tgccgaaata tttgcgcacc ataaggtcga ttatcctgtc gtcgggtcgc
     1681 cctcaaaggg gacatgcctg ctgaaccgcg aatatagaga aatatcccga atgtgcagtt
     1741 aacgaattct tgcggtttct ttcagcgccg ccaataccgc cagcccgtcg cgcaaggggc
     1801 gcggctcgtg tgtgcggatg aagtcagctc cacctgcggc ggcggcaagc tctgcagcga
     1861 gtgtcgcggc cccgacatcc cccggaccac ggcctgtgag cgcgcgcaga aaggatttgc
     1921 gcgaaacaga cagaagcacc ggcaaatcga agcgcagccg caattcatcg aaccgcgcca
     1981 gcaccgagag cgaggtttcg ggagcagccc ccagaaaaaa ccccatgccg ggatcaagga
     2041 caaggcggtt gcgtttgata ccggcacccg tcagcgccgc gatgcgcgcg tcaaagaacg
     2101 ccgcaatgtg atccatgatg tcgccagcgg gtgcctcgcg ccgatctgcc tgcccgtctt
     2161 gcaccgaatg cataacgacg agtttggcag atgatttcgc caattgcgga tagaacgcag
     2221 cgtctggaaa accgcgaata tcattgagat aggccacacc acgcgacaag gcataggctt
     2281 gcgtcgcggg ttgataactg tcgagcgaga cgggaatgcc atctgccttg agcgcgtcca
     2341 gcaccggcgc gatacgcgcg atttctgtgt cggacgaaac aggcgcggcg tcgggattgc
     2401 tggatgccgg accgaggtcg atcacatctg ccccctcggc catcagctta cgcgcctgcg
     2461 caatggctgc gtctggcgcc agataccggc ctccatcgga gaaactgtcc gaggttatgt
     2521 tgacgatgcc gaaaatgatg agcgatttat tcatgggggc ttctataata ataataatcg
     2581 agcatgagtc tcatacggat gctcgggtcg aaagggaatc cccaggcgag taacctgttt
     2641 gcggtgatcc gtttcatgat gatgtatctg tagcattttg gaaataaata tatcatctct
     2701 caataagtag atatatggcg cagtcggcgc ggccatcacc ggcattgtgc tgctcggcga
     2761 gtccgctaac ccgatgcgcc tggcgagtct ggcgttaatc gtactgggga ttattggtct
     2821 gaaactcagc actcactaac taccgggctg ttgtacccaa atcaacttac tgatatcaaa
     2881 cccttcccgg gtcgcgactg ccagcatctc ctgttttact tcgtcagaaa tagttggcgt
     2941 gcgggagagt atccacaggt agtcgcggtc cgggccgcaa accagcgcat ggcgatactc
     3001 ccgatcgagt gcaatgacgt tataaccgcc atagaaagga ccaaagaatg atactttcag
     3061 cgcagcgcga gttggcgcgc cggtaaagta cgctttccct tcactctgct gccacatttc
     3121 tctatcaggg ttatagcctt tattgatgac attcaggccg ccatcatccc gtaggctgta
     3181 tgttgcggtg actttctcca gtccacgttc aaagcggtga tcaaaacggg caatctcata
     3241 ccaggtaccg agataacgtt tagcatcgaa attattcact acggtcacac cacgcggcgg
     3301 cgtgggagaa ctgcaggcaa cgaccagaaa tgcagctgtt gctgcggcaa cgagagggag
     3361 caggcgcata attgtttcct tactggtttt tttctaagtg tagatgacag caaggaaaag
     3421 cggagaaaag gtccgaaaat tcggacccga tgaaatttta ctgtagagcg ttgagaatct
     3481 gccaggcggc ggtaactctc gctggattgg gatagttttt gttagccagc atcacaatgc
     3541 ccagctcttt ttctgggata aatgcgatgt agctgccaaa tccgccggtc gcgcctgttt
     3601 tatgcaccca tgatgaccgc acagcaggag ttggtggcat catcgctttt actggtcgtg
     3661 ccgccagcgc aattttactg tcgctgccgt taatgatgct gtcaggatct accggccagt
     3721 ccagcatttc ccagcccagg ccctgataca tatcgccggt ctgccagtag cgagattgcg
     3781 ccagttgtat cccttgttga agtgttttgt cgttgatatc acgcggattt atattgctcc
     3841 gcacccagcg agccatatct tcaattgttg atttcacacc gtaagcttca gcatctaaca
     3901 tccctggcga aacatgtact gccttacctt cgcgatatcc ccaggcgtaa ttcttttctt
     3961 ctgcgggcgg cacgttaatc cacgtatggg tgagtttgag tggctggaaa acacgagttt
     4021 gcatcgcctg ttcaaagctc aaaccagacg gcttcacagc cagtgcgcca aacaaaccaa
     4081 tactggagtt cgcgtataaa cgttgtgttc ctggagccca ttcaggctgc cagttttgat
     4141 agaaacgtag caggtcagtc gctgacttca cttcatccgg cacctgcaat ggcagaccgc
     4201 cagcggtgta ggttgcgaga tgtaatagcg tgatcccatt ccactgttta gcggtaagtt
     4261 caggccagta ttttgtgacg tgatcgctta ggttgatttc ccctcgtgca atggcgtcgc
     4321 cactaagcac gccggtaaat gttttactga ccgaacctaa ctcaaacaac gtttgctgcg
     4381 tgacgggccg cttttgtgtc acgtccgcat agccccaggt aaagtaataa ggcttgccct
     4441 gataaatcac agcaacggct atgccgggga ttttttgttg ctcaacaagt ggggtaatcg
     4501 tgcgatgcac aatatcgttg atttgttgtt cactgaagga ggcggcaagt gttgagcaag
     4561 aggcagtaat cagtaaggcg cagagcgtcg ttttgagcat aaggtctgat ttccatacaa
     4621 aacgctgatg aatcccctaa tgattttggt aaaaatcatt aagttaaggt ggatacacat
     4681 cttgtcatat gatcaaatgg tttcgcgaaa aatcaataat cagacaacaa gatgtgcgaa
     4741 ctcgatattt tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc
     4801 aacagcttaa cgttggcttg ccacgcctta cttgactgta aaactctcac tcttaccgaa
     4861 cttggccgta acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga
     4921 ttgttaggta atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc
     4981 tttatctgtt cgggcaatac gatgcccatt gtacttgttg actggtctga tattcgtgag
     5041 caaaaacgac ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt
     5101 tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta
     5161 gccgaccttg cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc
     5221 tttaaagtgc catggtataa atccgttgag aagctgggtt ggtactggtt aagtcgagta
     5281 agaggaaaag tacaatatgc agacctagga gcggaaaact ggaaacctat cagcaactta
     5341 catgatatgt catctagtca ctcaaagact ttaggctata agaggctgac taaaagcaat
     5401 ccaatctcat gccaaattct attgtataaa tctcgctcta aaggccgaaa aaatcagcgc
     5461 tcgacacgga ctcattgtca ccacccgtca cctaaaatct actcagcgtc ggcaaaggag
     5521 ccatgggttc tagcaactaa cttacctgtt gaaattcgaa cacccaaaca acttgttaat
     5581 atctattcga agcgaatgca gattgaagaa accttccgag acttgaaaag tcctgcctac
     5641 ggactaggct atatttatct tttttgcttc tttggtgctg caagtcacca atacgaggga
     5701 aacctgttac ggagcggttt aaccgtaatc aacagtgact ggtgtcatgt tcggaagatt
     5761 aattgatagt aatctacaaa ggatcttctt gagatccttt tcaatgaatc gtaatctctt
     5821 gctctgtaaa cgaaaaaacc gcctggggag gcggtttttt cgaaggttaa gtcagttggg
     5881 gaactgctta acccggtaac agggttcacg agaccgccat taccaaaact gttctttcag
     5941 tgtagccgca gtcaggcctc tacttcaaga cctcttaatc tatcttctcg tgcactcctg
     6001 ttactaatgg ctgctgccag tggcgttttg tcgtgtcttt ccgggttgga ctcaagttga
     6061 tagttaccgg acaaggcgca gcggtcggac tgaacggggg gttcgtgcat acagtccagc
     6121 ttggagcgaa ctgccttcac ggaactgagt gtcaggcgtg gaatgagaaa ccgcggccat
     6181 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcta
     6241 ccagggggaa acgcctggga tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     6301 cgtcagattt tgcgatgctt gtcagggggg cggagcctat ggaaaaacgg cttcgccgtg
     6361 gccttatctc ttctttgcta agtgtcttcc tggtatcttc caggaaattt ccgtccccgt
     6421 ccgtaagccg gtaccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag
     6481 gaagcggaat atctcctgta tcgcatattc tgctgacgcg ccggtgctgc cttttttctc
     6541 ctgccacatg aagcacttca ctgattttcg caatcgtggc aacatagtaa gccagtatac
     6601 actccgctag cgctacatga ctggttcagg gctgcgcccc gaaacccgct aacaacctct
     6661 cagctactgg atatcacccc atgccatctg aatatgatga acaactggat tcgactgata
     6721 cgcagcttcc cggcgaatat gacctttttg atttaatggc gcaggttact gaagagaacc
     6781 tgcatgcccc tgtggatttt gggtcgcctg tcggccggga agcgttatag ctttttcgct
     6841 tgcccgcgta cgtggtaata ctccggtatt accttttatt tctttatttt catgtaatta
     6901 cagtggattt ttaatacccg ggtattactc gtgaaaacga aatattcctt ttttttcttt
     6961 taattcatgc agttgcgatg aaaatcgcgg ctgcgttagg tgtatgacca ttttgaggtt
     7021 gaaaaataat caaccatggc gggtgattta cgcaggttaa ggggagatcg tgacaaaaaa
     7081 agcgttcctc actgtatcgt tcatgcctgc atgaacgggt tattcttcct gactacagaa
     7141 ttacatcgct ccttcagttc atacatcact tatacatcac ttttacatca cttttacatc
     7201 attccttcag ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct
     7261 gaacgtgctg acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa
     7321 ttgcgtcccc gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc
     7381 tgaatggtgt gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac
     7441 agagcctgcg cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc
     7501 gtcagaaacg ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag
     7561 agtcacctgc actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt
     7621 ttttcctgtg ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc
     7681 tcatttcgca ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca
     7741 gtctgctgca gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg
     7801 ctaacgacag atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc
     7861 tcgccatagg cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg
     7921 cgatagctga caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt
     7981 aatttcagtg tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt
     8041 accgtattgt aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt
     8101 gaggcgtgaa aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt
     8161 cgaatactgt atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa
     8221 gaggataatt ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg
     8281 ggaccaaatg acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt
     8341 aacggtggtg gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt
     8401 ggtctcatca ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc
     8461 gatctttctg ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac
     8521 ctagatactg cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca
     8581 acggcaggca tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa
     8641 acacagtatt gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga
     8701 tcgggatact ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt
     8761 tgacgagtat tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg
     8821 ctatgtgcaa cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct
     8881 acgggttcgc tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg
     8941 cggtttctgc ccgagctgtg gggcgcggcg gatggccgaa agtgc
//
