LOCUS       NC_008489               7462 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid pMG828-4, complete sequence.
ACCESSION   NC_008489
VERSION     NC_008489.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225669
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Shu,H.-Y. and Lin,G.-H.
  TITLE     Comparative analysis of five indigenous plasmids from a clinical
            strain of Escherichia coli with biofilm formation ability
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Shu,H.-Y. and Lin,G.-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-SEP-2006) Sequencing Core, Genome Research Center,
            National Yang Ming University, 155 Li-Nong St., Sec. 2, Peitou,
            Taipei 112, Taiwan
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009951295.2-RS_2024_06_24
            Annotation Date              :: 06/24/2024 02:52:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 8
            CDSs (total)                 :: 8
            Genes (coding)               :: 8
            CDSs (with protein)          :: 8
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            DQ995354.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MG828"
                     /db_xref="taxon:562"
                     /plasmid="pMG828-4"
                     /note="clinical isolate"
     gene            join(7436..7462,1..420)
                     /locus_tag="HS443_RS00005"
     CDS             join(7436..7462,1..420)
                     /locus_tag="HS443_RS00005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_137508464.1"
                     /translation="MEGSARRFSVSELHVSPGQKKTDQGSGQESESVRNFFSCGMRQCS
                     QPPQNTLRLIKNNWHYPVPDKKTTKVGNPRETIGVRLSQNIDNEIVTQINLAASLILIQ
                     YSIKNARIINHIYKDIYQTKQPSSQLRIRIFLYVIDKTQFFYKL"
                     /name="hypothetical protein"
     gene            complement(912..2570)
                     /locus_tag="HS443_RS00010"
                     /old_locus_tag="pMG8284_01"
     CDS             complement(912..2570)
                     /locus_tag="HS443_RS00010"
                     /old_locus_tag="pMG8284_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007372656.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_001529697.1"
                     /translation="MKIVIVGGVAGGASAAARARRLSEDVSIVVFERGSDVSFANCGLP
                     YHIGGKIPLRQSLILKTPEDFKSRFNIDVRIRHEVMSVDPVNKMVTVKNLTSGEVYTEE
                     WDRLLLSTGAAPVVPPLPGLQEAGVFTLRNLTDMDAIQAWIEQHNIAHTTLVGGGFIGL
                     EVMEALSERGISVTLLEMGEQVMAPVDPEMASALHQEIRSHGVDLRLRTALTEVLRTET
                     GFRVALSDGGFLQTDMVILAIGVKPESSLATGAGLAVGKRGGISVNACMQTSIPDIYAV
                     GDAVETPDLVFQEPANVPLAGPANRQGRIAADNMLDRHSLYHGSQGTSICKVFSLSIGS
                     VGANEKQLKAHGTRYEKVYVHAADHASYYPGATMISLKLLFSPDTGKILGAQASGKKGV
                     DKRIDVLAVVQRAGLTVNDLEHLELTYAPPFNSARDVVNQAGMVAANVIKGDTVICHVR
                     DVLQREPGSYCLLDIRSPAELKQFGEYPDALSIPLDSLRENLDKLPEDKEIIIGCQSGL
                     RGHVAYRMLQSHGFRTYNLSGGFITWQAVTESMSQ"
                     /name="FAD-dependent oxidoreductase"
     gene            2731..3078
                     /locus_tag="HS443_RS00015"
     CDS             2731..3078
                     /locus_tag="HS443_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329508.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_023144239.1"
                     /translation="MPMNEDTKRQTDMKNAALGAADVLRGLANSDRLLLMCQLSQGEAN
                     VTELEAAVGITQPTLSQQLGVMRRLKLVETRRAGKMVYYRIGDNRIMTLLNTLYDLYCP
                     QADPDKVKKQP"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3622..3843
                     /locus_tag="HS443_RS00020"
     CDS             3622..3843
                     /locus_tag="HS443_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023144240.1"
                     /translation="MTKLDENIPHIIDAAVDFMASSQAFREYLKKLPPDNTIPPDLTDE
                     HVPLYIRRLEYYRLLYRPKQTESYWKKR"
                     /name="hypothetical protein"
     gene            complement(3897..4430)
                     /locus_tag="HS443_RS00025"
                     /old_locus_tag="pMG8284_02"
     CDS             complement(3897..4430)
                     /locus_tag="HS443_RS00025"
                     /old_locus_tag="pMG8284_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_011666803.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4433..5359)
                     /locus_tag="HS443_RS00030"
                     /old_locus_tag="pMG8284_03"
     CDS             complement(4433..5359)
                     /locus_tag="HS443_RS00030"
                     /old_locus_tag="pMG8284_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_011666804.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAREHCFD
                     MRDGVSRENRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            5536..5934
                     /locus_tag="HS443_RS00035"
     CDS             5536..5934
                     /locus_tag="HS443_RS00035"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249654.1"
                     /translation="MRISNVEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQTIQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQELKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(6128..7063)
                     /locus_tag="HS443_RS00040"
                     /old_locus_tag="pMG8284_04"
     CDS             complement(6128..7063)
                     /locus_tag="HS443_RS00040"
                     /old_locus_tag="pMG8284_04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011666805.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPVPLSP
                     PECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAKRGRKGGIAKGEAYDDKRFMAL
                     CMLENGYSQKAIAAMLEVSTRTIRNWKSGK"
                     /name="replication initiation protein"
     Typing          complement(6129..7063)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.902"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4437..5359)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.078"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtttcagagc ttcacgtttc gccaggacaa aaaaaaaccg accaagggtc ggggcaagaa
       61 tcagagtctg tgagaaattt ttttagttgt ggaatgcgtc aatgttcgca accgccccaa
      121 aataccctga ggctgataaa aaacaactgg cactatcccg ttccggataa aaaaaccaca
      181 aaagtgggga atccaagaga aacgataggt gtcagattga gtcagaatat tgacaatgaa
      241 atagttacgc aaataaattt agctgcatca cttattctga ttcaatactc aataaaaaat
      301 gcccgcataa taaaccacat ttacaaagat atatatcaaa cgaaacaacc aagttcacag
      361 ctcaggataa gaatttttct ctatgtaata gataaaacac agttttttta taaactgtaa
      421 tatatgagct gtaatattca tgtattaatt gtttttttga aaagcatcac aaatttattt
      481 tttacgcata ctgccctatt gcaaccagca atggttttca cttattttca acatctcatt
      541 taatacgagt tatacttcaa cccgtttaaa gtgcaacact aactgagtat catttgtttt
      601 ccttacctac ccctggattc ccaggggttt tttataacta caaaccagca caacaaaaaa
      661 acaaaacaca ataaaaacaa tgaattatat aaaactcaac gatatctttg tacggagatt
      721 tacattcagg aacattgggg tcagtttgga tatagagaat tattgtacgg taagcctgtt
      781 ttttgaacag tccacattat cgcaataggg taccatccag ggtgttaatt atttttgtag
      841 atttctattc catataatac gttttcaatg aatctggaag atgaggtgcc atgtgtatgg
      901 cacctcatct cttattgact cattgattcc gtaaccgcct gccaagttat aaagccacca
      961 ctcagattat atgtccggaa gccgtgagac tgaagcatac ggtatgccac atgcccccgc
     1021 agaccactct gacacccgat aataatctct ttatcttcag gcaacttatc aagattctcc
     1081 cgcagggaat caagagggat ggatagcgcg tcaggatatt caccaaattg tttcagttct
     1141 gcaggagaac gaatatcaag taaacaataa ctgcccggtt cccgttgtaa cacatcgcgg
     1201 acatgacaga tgaccgtatc gcccttgata acatttgccg ccaccattcc agcctggtta
     1261 acgacgtccc ttgcactgtt gaaaggcgga gcataggtca gctccagatg ctcgagatcg
     1321 ttaacggtaa gccccgcgcg ctgtacgaca gccaggacat cgatgcgttt atcaacccct
     1381 tttttccctg acgcctgggc cccgagaatt ttaccggtat cggggctgaa cagcagtttc
     1441 agactgatca ttgttgcccc cgggtagtaa ctggcatgat cagcggcatg gacgtaaacc
     1501 ttctcgtatc gggtaccatg ggctttcagc tgcttttcat tcgccccgac actgccaata
     1561 ctcagcgaaa ataccttaca aatggatgtc ccctgactgc catggtaaag gctgtgacga
     1621 tcgagcatat tatcagcagc aatgcgtccc tggcggttgg ctggccctgc cagaggaaca
     1681 ttagcgggtt cctgaaagac aaggtcaggt gtttcaactg catcccccac ggcatagatg
     1741 tcagggatac ttgtctgcat acaggcattg acacttatgc ctccccgttt acccacggcg
     1801 agcccggctc ctgtggcaag gctgctctca ggttttaccc cgatggcgag aataaccatg
     1861 tcggtctgca gaaacccacc gtcagacagt gcaacgcgga atcccgtctc tgtccgcagt
     1921 acctcggtga gcgccgttct gagacgcaga tccacgccat gactcctgat ttcctgatgc
     1981 agggccgatg ccatttcagg atcaaccgga gccataacct gttcccccat ctccagcagg
     2041 gtcacactga tccccctttc gctcagtgcc tccatcactt cgagcccgat aaatcccccc
     2101 ccgacaagcg tggtgtgagc gatattatgt tgctcaatcc acgcctggat agcatccatg
     2161 tcagtaagat ttcgcagtgt aaagacaccc gcttcctgta gtccgggcaa ggggggaaca
     2221 accggagcgg caccggtact gaggagcaga cgatcccatt cttcggtata gacctccccg
     2281 gaggtcagat ttttaaccgt caccatctta ttaaccgggt ctactgacat cacttcatga
     2341 cggatacgga cgtctatgtt aaaacgggat ttaaaatcct ccggggtttt cagaatcagc
     2401 gactgccgta acggaatttt tccgcctata tgataaggca atccacagtt ggcgaaagag
     2461 acatcgcttc cccgctcaaa cacaacaata ctgacatcct ctgataaccg acgagccctg
     2521 gctgcagcgg atgctccccc ggcaacacca ccaacaatca ctattttcat tacatcctct
     2581 ctgttcactc ggggcaaaat atctgccata tataattagt tacccggtcc agctggacgc
     2641 tgcagacctg aagatcacgg tggcaaaatt acgccactat cattatactg aacaatatta
     2701 tataaaaata cataatataa gaaaccagca atgcccatga atgaagacac aaaacgtcag
     2761 actgatatga aaaatgcagc cctgggggca gcagatgtcc tacggggact ggccaacagc
     2821 gaccggctat tgttgatgtg tcagttaagc cagggggaag cgaatgtgac cgagctggaa
     2881 gcggcggtcg gtatcactca gccgaccctg tctcagcagt tgggtgtcat gcgtcgcctg
     2941 aagctggtcg agacccgacg ggcaggtaaa atggtatatt accgtatcgg ggataaccgc
     3001 atcatgacgt tattgaatac cttatatgat ctctactgcc cgcaggcgga ccccgacaag
     3061 gtcaaaaaac aaccttaacg tacaataatt ttcgatatcc aaactgcccc caacattcac
     3121 cagcacagtt agacattaac aatgcaaaat caatgcgatt ccggtgccgt atgtcaggga
     3181 gaattatgta aacggggggg ggccggaatc gcattccagc tcatatcatt aaatacagaa
     3241 aaaaacctct gagaacacag aggggataag gaatatgaat actccggtca ggatattatc
     3301 ctagctttta tatacaacat gctcacaagg acaacaacta gctagttaat agatattaaa
     3361 aattttagag ctgaaacctt aaagcttgct tccattccat gttttattat aaaccttatc
     3421 ttgaaaactg aaaggaagta cggcagatta attaagttgc gtgcattctt ttcacgttat
     3481 aatacaaaca agaacacctg cgaagttatt tctccttaca tatcccccta taccaacggg
     3541 ggattttttc tggttcacta acgcataaat aatagtgaca tactataatt cacgaaccgt
     3601 gataatatca gatcaatcat catgacaaaa ttagatgaaa atatcccaca tattatcgat
     3661 gcagctgtag attttatggc atcaagccag gcatttcgtg agtatctgaa aaaactcccc
     3721 cctgataaca caataccgcc agatcttact gatgagcacg taccgttata tatacgaagg
     3781 ctggaatact atcgactgct ttatcgacca aagcagacag aatcatattg gaaaaaacgg
     3841 tgaacggttc gcgaaatcct ttcgcgctcc gtgaggcgtt ggacaagcga agcgcgtcag
     3901 tttttccggc tcttccccgt gacaggtttc tccaccagtt tatcttccag tgtcacaact
     3961 ttctgataca ccgccgctgt ggcgttgtag ttcctgtcga gtattgtttt cattccccat
     4021 ccgagcacac agaacataac cagaaaagcc actgacaccc cacccagcca tttccagcta
     4081 taccctttaa gggtgttgta ttcctgcacg atattgctgt gaattgtcgc aatatccctg
     4141 ttcagggcat ccgcgtttgc cttcatgttt gcgactgact cttccagccg ctgaaaatcc
     4201 tggctgatgc gctcacctat cagtcctgcc acgtccagct tctgcacctc gtttctgact
     4261 tcatccctga cctgttttgc cacaccgtca gaaatatccc gatatccgcg aagagtttcc
     4321 cttgccacag ccagagaatc agcaacagct ttctgttgct ctattgcggt atgttgcaga
     4381 tgctccaccg ataacaggag cgtgagtaat ttttcttctg tgctggccat gttcagaatc
     4441 ccatagaaaa tccacgtgac ggtgatttaa cccgttcagg ttcagttcgg ttctctctgc
     4501 tcactccgtc tctcatgtca aagcaatgct cccgcgcaac atcatgttgc agccttctga
     4561 ccagttcatg ttgcttaacc ggtgtatcca gccattccct gatgtcatgc ccgaccattc
     4621 gcgcctgttc aagatggcgg gaatagtccg gttcagaccg tttttgcatg atgtcatgac
     4681 gctgggcatc atcaagtcgt ccggcagctt caaaacccag atgcacttcg ggacgtcgtt
     4741 caatgccctg ttctttcagt gtccggcagt caatgaagcc cctttcaaag ccatgctttc
     4801 tcatatgttc gttgtgcttc acctcccagc gttttcgcaa ttcccgcaac tgctcttttt
     4861 gctgagtggg agtcaggctt ccggaatcct ttttcgcacc tccccgctcg ggatacctgg
     4921 cgttatagcg ccggaaatac tgttccggtg tacggtcaat gccatcattc acacgctgac
     4981 tcatcatgat atgcgcatga ggctgttcgc cgccgtcaat gctcgctttc ggattgtgaa
     5041 tggcaaaact ccaggcgtgc cgttcgccgg cttcctgacg tacaaaatcc tgcaccagtt
     5101 caaggcgctg ttccggtgtc agttcacggg gtaatgcaat ttcaagttcc ctataggtcg
     5161 aaccgttcgc acgttcaaac tggtcagccg cctgccagaa atgggaagga ttatccctgg
     5221 cccattccgg catgtttccg tgtgcggaat attccaaatc ctgacggtcg cggtatttct
     5281 ctttgcgctc gatgtaatcg gcgtggtttg cagcctgccc cttgccacca aatttaacgc
     5341 tgaggtgata ggttgccacg gtttcagccc tccgcaggag cgcactgttt ctcgacgaag
     5401 gagagaaaca tataagtgcg cttccttcta attattcgaa ttagcacgga agataccccc
     5461 tgcacccgcc gaccgcaagc gatatactgc acgaccacaa accgccgtaa aaaaacacca
     5521 gggagtacca caaacatgcg catcagcaac gtcgaatggc tgaagaaacg gatcggattc
     5581 atcaggaagc tcggagaaca gaccgcgcga cagagacaga ttattgacct gctcgacaac
     5641 gaagccggac tcacagaaca ggaacgcaaa cttctccacg tgctggcaac agcggaaaaa
     5701 aacgacttac aggcacagga gaacgagcga aaacagacga tccagaaacg aatcgaggga
     5761 aaaaaacaac gacgggaacg caatcaccga ctctttctcg cggctgggct actgatcgaa
     5821 gcaggactgg tagacacaaa aacaggcgag ctatgctata aaaaagacag gatattacag
     5881 gaacttaagg aaattaagta cgatctggag acatccccaa acccagacgc gtaagtgcct
     5941 ccatccgtgt tccggttccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg
     6001 agactgcggc gagcactacc agcacaatca tgcggaaaac agcaccattg cagggctgaa
     6061 cggggaaaaa cggcaaaaga cctgctgctc aaaagaacgc cagaaaggcg ctgttatctg
     6121 atataggcta ttttccgctt ttccagtttc gaatggttcg agtagaaacc tccaacatcg
     6181 ccgcaatagc tttctgagaa tatccattct ccagcataca tagcgccatg aaacgcttgt
     6241 catcgtaggc ttcgccttta gcgatgccac ctttcctgcc tctcttggcc tgtatttctg
     6301 gcgtgtgcgt atcagccaca tactgtgcga aagtttccgg cgtgaagttc ctgtgcgtgt
     6361 atttcgcaat actcttgcca atagcccgac attcaggagg tgaaagcgga acgggaagcg
     6421 atgcgttgta catttcgaca cgctgaatca cggcatcaag ccactgtgag aatgctggcc
     6481 agccctgacg aatcgccctg taagcccatt tgcgcgtcat ttcgaacagg tggcagtttc
     6541 gccccatccc gtaatgttta tcgatgctac gacgcgctga ggcgctcaaa tcgagataat
     6601 cagccagttc atcgagggta taggcttcct cgcgccactc catcaccagc cattccaggt
     6661 ggaacggatt tttgcaaatc aggccgctgt agttcacatc cgcacaaagt ttctcacaca
     6721 gcgaacgttc aaccgctgcg gcatatttca gcgccttaac cgaggcatca ggcgcggttc
     6781 tcacggcgat gttgagtgcg tagagcaggt gagcgtgtcc gttcacagga tttttaacgg
     6841 tgatgttcgg cgcgggagcg ttccggtcgc tccagtcaat cgcagccccc tcgcggtcaa
     6901 cgtcaaaaac cagccagaac tgtgcatgag gctggttctg ctggatatac cgtgcaagaa
     6961 gcgcgcgccc tttgccggag atacgaaggc cgaaagcgag atcatccgtg tgatagggac
     7021 ggtggggtaa attttcttcg aagtattgaa gcgcggcgct catgtgttac gattccttcc
     7081 aggttatcga ccgacatcgc ccctgtattt tcgtgaaagt cttggcggat agacacgaat
     7141 ttcaggggcg tttgtttttc agtcgcttac actacaccca acggcaaatc ctgtcgatcc
     7201 ttcccgaaaa accgatccat ttaccataaa tcccgttgta cgcccccctt aaaacgccct
     7261 cagtcgcttt ctggcgcaaa aaacgcaaaa agacaagaaa acaccaggca aaaccagaaa
     7321 aacgcgtcag aatcgttttc agggcgttgc ataactatgc acgaaagcga tgtgcgatcg
     7381 gtttcgtgcg ttctgaacac cgaacggctc aaaacgaaaa gttttctccc cccggatgga
     7441 aggttctgcc cggagatttt ct
//
