LOCUS       NC_007768               2361 bp    DNA     circular CON 05-MAY-2024
DEFINITION  Staphylococcus chromogenes plasmid pLNU1, complete sequence.
ACCESSION   NC_007768
VERSION     NC_007768.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14229156
KEYWORDS    RefSeq; lincosamide nucleotidyltransferase; lnuA gene; rep gene;
            replication protein.
SOURCE      Staphylococcus chromogenes
  ORGANISM  Staphylococcus chromogenes
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1
  AUTHORS   Luthje,P., von Kockritz-Blickwede,M. and Schwarz,S.
  TITLE     Identification and characterization of nine novel types of small
            staphylococcal plasmids carrying the lincosamide
            nucleotidyltransferase gene lnu(A)
  JOURNAL   J Antimicrob Chemother 59 (4), 600-606 (2007)
   PUBMED   17329268
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Schwarz,S.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2006) Schwarz S.P., Molecular Microbiology and
            Diagnostics, Institute for Animal Science (FAL), Hoeltystr. 10,
            Neustadt-Mariensee, 31535, GERMANY
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009944555.2-RS_2024_05_05
            Annotation Date              :: 05/05/2024 01:19:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 2
            CDSs (total)                 :: 2
            Genes (coding)               :: 2
            CDSs (with protein)          :: 2
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AM184099.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Staphylococcus chromogenes"
                     /mol_type="genomic DNA"
                     /isolate="TS1"
                     /isolation_source="dairy cattle subclinical mastitis"
                     /db_xref="taxon:46126"
                     /plasmid="pLNU1"
                     /geo_loc_name="Germany"
     gene            91..1014
                     /locus_tag="HXE79_RS00005"
                     /old_locus_tag="pLNU1_01"
     CDS             91..1014
                     /locus_tag="HXE79_RS00005"
                     /old_locus_tag="pLNU1_01"
                     /function="essential for plasmid replication"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002501032.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_011428876.1"
                     /translation="MDKYTEKKRRNQVFQKFIKRHVGENQMTLVRECNTFLSFVTDKSL
                     EKNKLYKANSCKNRFCPVCAWRKARKDALGLSLMMQYVQKEHEKEFIFLTLTTPNVSKN
                     KLENEIKHYNQSFRRLSNRTKFKKVVKGYVRKLEITYNKERDDYNPHFHVLIAVNKSYF
                     KDTKSYISQKEWLNLWRDVTGMPEITQVHVEKVKANNDKELYEMAKYSGKDSDYLSNQK
                     VFDAYYRSLKGKQVLVYSGLFKEARKLLKNGDLDYLKEIDPTEYIYQIFYIWKQKEYLA
                     SELYDLTEQEKRELNHQMIDEIEEET"
                     /name="protein rep"
     gene            complement(1701..2186)
                     /gene="lnu(A)"
                     /locus_tag="HXE79_RS00010"
                     /old_locus_tag="pLNU1_02"
     ARG             complement(1705..2186)
                     /name="lnu(A)"
                     /gene="lnu(A)"
                     /gene_name="lincosamide nucleotidyltransferase Lnu(A)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1702..2186)
                     /name="lnuA"
                     /gene="lnuA"
                     /gene_name="lincosamide nucleotidyltransferase (LNU)"
                     /tool="rgi"
                     /drug_class="lincosamide antibiotic"
                     /identity="93.79"
                     /coverage="100.0"
     Typing          92..1014
                     /name="rep_cluster_1230"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tagtctaaaa acattatgag aaaggagaaa aagcaacttt ttttattgtc atagtttgtg
       61 aaaactaagt tgttttttaa gttatataac atggataagt atactgagaa gaaacgaaga
      121 aatcaagtat ttcagaaatt tataaagcga catgtggggg aaaatcaaat gacattggta
      181 cgggaatgta ataccttttt gtcttttgtg acagataagt cgttagaaaa aaataaatta
      241 tataaagcaa attcatgtaa aaatcgtttt tgtccggttt gtgcatggcg taaagcgaga
      301 aaagatgcgt tgggtttatc tttgatgatg cagtatgtac aaaaagaaca tgaaaaagaa
      361 tttatatttt taactttaac gacgcctaat gtgtctaaaa ataaattaga aaatgagatt
      421 aagcattata atcaatcatt tagacgatta agtaatcgaa caaaatttaa aaaggttgtt
      481 aagggttatg taagaaaatt agaaattaca tacaacaaag agcgggatga ttataaccca
      541 cattttcacg ttttaattgc ggttaacaaa tcttatttca aagatactaa atcttatatt
      601 agccaaaaag aatggttgaa tttatggcga gatgtgacgg gaatgccaga aattacacaa
      661 gttcatgtcg agaaagttaa ggctaataat gacaaagaac tttatgaaat ggctaaatat
      721 tctggtaaag atagtgatta tttaagtaat caaaaagtat tcgacgcgta ttatcgttct
      781 ttaaaaggta agcaggtatt agtgtattcg ggattattta aagaggcgag aaagttatta
      841 aaaaatggcg atttagatta tttaaaagag attgatccaa ctgaatatat ctatcaaatt
      901 ttttatattt ggaaacaaaa agaatattta gcgagtgaat tgtatgattt aacagaacaa
      961 gaaaaaagag aattaaatca tcaaatgatt gatgaaattg aagaagaaac ataaagtgtt
     1021 ggtcttagac tgacactttt ttattgttaa atatatggtc gcctgcctct gtgggaacta
     1081 taaaaaatgt atgcaaatta atttgcatgt agatgggcag tattgaaact aatactgata
     1141 agttacaacg atattaggtt gattttaaaa ttgttgaacg tgtccgattt ctacaagcta
     1201 agagtaaata cagaaaaggc tacatttaag gtacaaaaag cttacaagtg atttacgatt
     1261 taaatgtagt taagttgtat cgttttggtt aataaaaatg tagtgttata acatgctttt
     1321 atttggctca tatttgcgtt ttaagagctt atgtatgttt ttaggtataa aactatatga
     1381 tttacactta aaactttaaa ataccccttt agaattaaaa ttaagggcat ttaagattta
     1441 acaaaataaa aaaagccacc aataaaattt ggtggctttt tttatttttg gaagtgaaca
     1501 gtgaaaacaa aacagaacgt taattaaatt ataacacgct tttatgccga gaaaatttat
     1561 tgatcgttga gaagaaccct taactaaact tgtagacgaa tgtcggcata gcgtgagcta
     1621 ttaagccgac cattcgacaa gttttgggat tgttaagggt tccgaggctc aacgtcaata
     1681 aagcaattgg aataaagcaa ttatgttatt gattttaaat tttttatatc aaaatggtct
     1741 ttttctgtta agtcataacc agaatgaaaa agaagctgag cttcttttga aatacatggt
     1801 atttttcgac ctttataatt agtttctgaa aaccattcat tttgaaaaac gtaattgcca
     1861 ccttctggat ttgcttgagt aatggaacca tcatcattta aatttatggg atgaatatct
     1921 aaatatccat attttttatg tttaagttcc atgcgtgaag gcatccaatc aacttctatt
     1981 ttatatccta tatcttctaa tttttgtata actttttgag tgtgttgggc gtcaaaatct
     2041 atatctatat ctctgtgttc tctttgttgt tttccagtta atacatctac accccaacct
     2101 ccatctaacc aataagttac ttccattttt tcaaataagt ctaaaatata aaataaatcc
     2161 ttttctgtta cattattaat tttcatttta tccctcctaa tttaggaatt tgaaacgagt
     2221 tgaaaacgag tttcttcttg tcttgataat aagggtaact attacagagt agaatagtta
     2281 cccttaaagt gttggtatat ctgggttgaa acacaaaaaa agttgctttt tttagcatac
     2341 tcatgtagtg ttttaggtgt c
//
