LOCUS       NC_005951              20652 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Staphylococcus aureus subsp. aureus MSSA476 plasmid pSAS, complete
            sequence.
ACCESSION   NC_005951
VERSION     NC_005951.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMEA1705922
            Assembly: GCF_000011525.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus subsp. aureus MSSA476
  ORGANISM  Staphylococcus aureus subsp. aureus MSSA476
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20652)
  AUTHORS   Holden,M.T., Feil,E.J., Lindsay,J.A., Peacock,S.J., Day,N.P.,
            Enright,M.C., Foster,T.J., Moore,C.E., Hurst,L., Atkin,R.,
            Barron,A., Bason,N., Bentley,S.D., Chillingworth,C.,
            Chillingworth,T., Churcher,C., Clark,L., Corton,C., Cronin,A.,
            Doggett,J., Dowd,L., Feltwell,T., Hance,Z., Harris,B., Hauser,H.,
            Holroyd,S., Jagels,K., James,K.D., Lennard,N., Line,A., Mayes,R.,
            Moule,S., Mungall,K., Ormond,D., Quail,M.A., Rabbinowitsch,E.,
            Rutherford,K., Sanders,M., Sharp,S., Simmonds,M., Stevens,K.,
            Whitehead,S., Barrell,B.G., Spratt,B.G. and Parkhill,J.
  TITLE     Complete genomes of two clinical Staphylococcus aureus strains:
            evidence for the rapid evolution of virulence and drug resistance
  JOURNAL   Proc Natl Acad Sci U S A 101 (26), 9786-9791 (2004)
   PUBMED   15213324
REFERENCE   2  (bases 1 to 20652)
  AUTHORS   Holden,M.T.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUN-2004) Submitted on behalf of the Pathogen
            Sequencing Unit, Sanger Institute, Wellcome Trust Genome Campus,
            Hinxton, Cambridge CB10 1SA, E-mail: mh3@sanger.ac.uk
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 01:05:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,788
            CDSs (total)                      :: 2,707
            Genes (coding)                    :: 2,644
            CDSs (with protein)               :: 2,644
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 43 of 63
            Pseudo Genes (incomplete)         :: 27 of 63
            Pseudo Genes (internal stop)      :: 13 of 63
            Pseudo Genes (multiple problems)  :: 17 of 63
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BX571858.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20652
                     /organism="Staphylococcus aureus subsp. aureus MSSA476"
                     /mol_type="genomic DNA"
                     /strain="MSSA476"
                     /sub_species="aureus"
                     /db_xref="taxon:282459"
                     /plasmid="pSAS"
     gene            12..638
                     /locus_tag="SAS_RS14135"
                     /old_locus_tag="pSAS01"
     CDS             12..638
                     /locus_tag="SAS_RS14135"
                     /old_locus_tag="pSAS01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            876..1505
                     /gene="mobV"
                     /locus_tag="SAS_RS15260"
                     /pseudo=""
     CDS             876..1505
                     /gene="mobV"
                     /locus_tag="SAS_RS15260"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            1599..2144
                     /locus_tag="SAS_RS14150"
     CDS             1599..2144
                     /locus_tag="SAS_RS14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000273005.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     gene            complement(2179..2487)
                     /locus_tag="SAS_RS14155"
                     /old_locus_tag="pSAS03"
     CDS             complement(2179..2487)
                     /locus_tag="SAS_RS14155"
                     /old_locus_tag="pSAS03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001020850.1"
                     /translation="MNIHSHLKTFIHNKLLYYQCQTIKGVQLFKTLLLCGKNTPFMWQK
                     HSFYVAKTLLLCGKNTPLGIEIPCCSRAEQAPKKVFKKIYKNILKSSRGLPIFHPKK"
                     /name="hypothetical protein"
     gene            2527..3387
                     /locus_tag="SAS_RS14160"
                     /old_locus_tag="pSAS04"
     CDS             2527..3387
                     /locus_tag="SAS_RS14160"
                     /old_locus_tag="pSAS04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000145511.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            3474..3815
                     /locus_tag="SAS_RS14165"
                     /old_locus_tag="pSAS05"
     CDS             3474..3815
                     /locus_tag="SAS_RS14165"
                     /old_locus_tag="pSAS05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(4054..4125)
                     /locus_tag="SAS_RS15085"
     CDS             complement(4054..4125)
                     /locus_tag="SAS_RS15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            4274..4459
                     /locus_tag="SAS_RS14170"
                     /old_locus_tag="pSAS07"
     CDS             4274..4459
                     /locus_tag="SAS_RS14170"
                     /old_locus_tag="pSAS07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(4678..5421)
                     /locus_tag="SAS_RS14175"
                     /old_locus_tag="pSAS08"
     CDS             complement(4678..5421)
                     /locus_tag="SAS_RS14175"
                     /old_locus_tag="pSAS08"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843757.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEAKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     gene            5832..6508
                     /locus_tag="SAS_RS15150"
                     /old_locus_tag="pSAS09"
                     /pseudo=""
     CDS             5832..6508
                     /locus_tag="SAS_RS15150"
                     /old_locus_tag="pSAS09"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            <6519..6801
                     /locus_tag="SAS_RS15265"
                     /old_locus_tag="pSAS10"
                     /pseudo=""
     CDS             <6519..6801
                     /locus_tag="SAS_RS15265"
                     /old_locus_tag="pSAS10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            8010..8627
                     /gene="cadD"
                     /locus_tag="SAS_RS14190"
                     /old_locus_tag="pSAS11"
     ARG             8011..8624
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            8646..8993
                     /locus_tag="SAS_RS14195"
                     /old_locus_tag="pSAS12"
     CDS             8646..8993
                     /locus_tag="SAS_RS14195"
                     /old_locus_tag="pSAS12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            9162..9254
                     /locus_tag="SAS_RS15095"
     CDS             9162..9254
                     /locus_tag="SAS_RS15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            9466..10440
                     /locus_tag="SAS_RS14200"
                     /old_locus_tag="pSAS13"
     CDS             9466..10440
                     /locus_tag="SAS_RS14200"
                     /old_locus_tag="pSAS13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072388.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLIQSVPSEIKENSGWI
                     RVQDCGKNVKKIKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(10560..11375)
                     /locus_tag="SAS_RS14205"
                     /old_locus_tag="pSAS14"
     CDS             complement(10560..11375)
                     /locus_tag="SAS_RS14205"
                     /old_locus_tag="pSAS14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164957.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKCQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(11368..12811)
                     /locus_tag="SAS_RS14210"
                     /old_locus_tag="pSAS15"
                     /pseudo=""
     CDS             complement(11368..12811)
                     /locus_tag="SAS_RS14210"
                     /old_locus_tag="pSAS15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /name="Mu transposase C-terminal domain-containing protein"
     gene            complement(12783..13376)
                     /locus_tag="SAS_RS14215"
                     /old_locus_tag="pSAS16"
     CDS             complement(12783..13376)
                     /locus_tag="SAS_RS14215"
                     /old_locus_tag="pSAS16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(13640..14020)
                     /gene="blaI"
                     /locus_tag="SAS_RS14220"
                     /old_locus_tag="pSAS17"
     ARG             complement(13644..14020)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(14010..15767)
                     /gene="blaR1"
                     /locus_tag="SAS_RS14225"
                     /old_locus_tag="pSAS18"
     ARG             complement(14014..15767)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            15874..16719
                     /gene="blaZ"
                     /locus_tag="SAS_RS14230"
                     /old_locus_tag="pSAS19"
     ARG             15875..16716
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            17088..17621
                     /locus_tag="SAS_RS14235"
                     /old_locus_tag="pSAS20"
     CDS             17088..17621
                     /locus_tag="SAS_RS14235"
                     /old_locus_tag="pSAS20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001823239.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNESK
                     RRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEVNIT
                     RQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            18035..18328
                     /locus_tag="SAS_RS14240"
                     /old_locus_tag="pSAS22"
     CDS             18035..18328
                     /locus_tag="SAS_RS14240"
                     /old_locus_tag="pSAS22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            18403..20337
                     /locus_tag="SAS_RS14245"
                     /old_locus_tag="pSAS23"
     CDS             18403..20337
                     /locus_tag="SAS_RS14245"
                     /old_locus_tag="pSAS23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000713729.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            20341..20646
                     /locus_tag="SAS_RS14250"
                     /old_locus_tag="pSAS24"
     CDS             20341..20646
                     /locus_tag="SAS_RS14250"
                     /old_locus_tag="pSAS24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831611.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKIFLAF"
                     /name="YxeA family protein"
     ARG             15875..16719
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             13035..20652
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6859..7006
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          2528..3387
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4679..5421)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          877..1151
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 ggtgaccgat agtgataaaa cttgaaaatg tttttgtaaa aaaaggtaat aaaaacattt
       61 tagacggatg taactttaat tttgaaaaag gaaaatcata tgctttagtt ggagaaagtg
      121 gcgctggaaa atctacttta cttaacataa tcgctgggtt cgaagatgta tcacaaggaa
      181 gcatatatat tgaagataag ttactaaaaa agaaagtcga tttttataga tatacgttag
      241 ggtatttgtt tcaaaatttt gctttacttg aaaatcaaac aatcagtcaa aatttagatt
      301 tagccctaaa gttcaaaaag aataaaaaag ataatatgaa cttaaagaaa gaagtattaa
      361 aaaaggtcgg cctagattta gatatcaaga gaattgttag ttctctaagc ggtggtgaac
      421 aacaaagagt agcattggca agattaatat taaaagatcc gaaaatcatt ttagctgatg
      481 aacctactgg gtcactagat acgaaaaatg gaaaaatagt tattgattta ttattgaagc
      541 tacttgatga aaataaaact atgattgtcg taacgcatga tttagagctt gcgaaacgat
      601 ttgatgtcat agtcaatatt tctgagctta gaagttaaga aatattgata ggtatagcca
      661 tacatagaga aaaaacaata tgaaatatga ttagtcatta cttttgagtt ttttaattta
      721 aggactggat tttccagtcc ttttttatga tgataaaaaa caagcctcgc agagcacacg
      781 tttttaatga cttatttggt ttttaataag tctagtatgt taaaactttt ctgtgtttag
      841 gtgttataat catatgcagt ggaagttgat ttattatgtc ttattcaatt attcgagttg
      901 ttaaagtgaa atcaaaaaca aatacaagag gtattcaaag acatattcaa agagaaaata
      961 aaaattatga aaatatagat attgatttaa gtaagtcata tttaaactat gatttagtca
     1021 atgatactaa gtttgatttt aataaaaaaa ttgatgagaa aatcgaaaaa aattataaag
     1081 ggaaacgcaa aattagaaca gatgccatta aacatattga tggattaatt acatcagata
     1141 atgtgttttt taatcaatta tctgaagaag agacaaaata gttttttaag catgctaaag
     1201 cgttcttgga agaagaatac ggtaaagaaa atcttttata tgcgacagta catatggatg
     1261 agataacacc acatatgcat tatggcgtag ttcctattac taaagatgga cgtttaagtg
     1321 cgaaagaagt tgtaggtaat aaaaaagcat taaccgaatt ccaagataga ttcaatacgt
     1381 atattaataa gcaaggctat gatttaaaac gaggaatttc tagacaactt acaaaagaaa
     1441 aacatgatca agttagccgt tataaacaaa aaacagaata tcataaacaa atgcatatgc
     1501 gctagaagca aatagaggat tatttaaagc aagaaaatga taaattgata caaaactacc
     1561 aaaaatcatt agatacgctt aaaaagcttc tgagtgtcat gtatgaaatt aaaactaaaa
     1621 atgtaggtgg atggtttcat aaagagaagc aagaaacagg caatattgta attactaaaa
     1681 ctgattttga aaaatacact aaacaaatta aagcagcaca gatgatatta gatgattatg
     1741 aatgtattaa aagtgggaag tcattaaaaa aagcagagaa acaaaatgaa agtttagtta
     1801 atgaattgac tagtgtccat atggaaaatg aaaagctggt tgaagaattt aatgatttag
     1861 cacaacgtta taattatttg ttgtcagaga atgaaaagaa agataaagaa ctaaattata
     1921 cgcttaagtt attcaatcaa gtttttaaaa tcattaaaag tatgatgaaa gaagagcgtt
     1981 atcatacact aataaatcat attgataatc atttagacaa ttctaaaata cgtgaagtaa
     2041 tgacgataga taacaatgat gaacagttct ttaagaagaa atatcaagca caagaacgag
     2101 aaatcatttt taaagaagat agagaagatg gttatacatt ataaaaatag atatttaaat
     2161 gcatttgttt gaggttaatt attttttagg atgaaaaata ggtagccccc tcgaactttt
     2221 taatatattt ttataaatct ttttaaaaac ctttttaggg gcttgttcag ccctagagca
     2281 acaagggatt tcaatgccta aaggagtgtt tttgccacat aaaaggagtg tttttgccac
     2341 ataaaaggag tgtttttgcc acataaaagg agtgtttttg ccacataaaa ggagtgtttt
     2401 aaaaagttgt actcctttta tggtttgaca ttgataataa agtagtttat tgtgaataaa
     2461 agttttcaaa tgggagtgta tattcaaacg atactccttt aggttcgtaa taaagggggc
     2521 tgtatcatga caggagagac agttgtatat aaaaatgaaa tgaatttagt tccattaagg
     2581 cgatttacag ctactgaaat taatttattt tttgcgatgt gtaataaatt aaaagagcaa
     2641 gatactaata cattacgttt gtcttttgat gaattaaaaa aattaagtaa ttatagtcca
     2701 gaaacacgta atattaatag atttgctaat gatttagata atgtgtataa aaagatgttg
     2761 aatttaacca ttagatatga agatgatgat gtaatagaaa gattcgtttt attcaatcat
     2821 tatagaatcc ataagcgaga acaatatcta gaaatatcaa cttcttcaaa tttaaaacac
     2881 atattgaatt caattactaa taattttaca aaatttgaat taaaagaaat gactaggctt
     2941 aaatctacat attctaaaaa tatgtttaga ttacttaaac aatacaaaca tacaggctat
     3001 ttaaaaattc acatcgatga ttttaaaaat cgtttagata tacctaaatc ttatcgtatg
     3061 actgatatta acaaaaatgt ttttaaacct attattattg aattaggttc tatatttaat
     3121 aatcttacta tcaataaaat caaagcgaaa aaagggcgta aaattgaatg gatagagttc
     3181 acatttgacg ctgagaaacg cattcataac aaacgacaac ctcaaatgtc caagatagat
     3241 aaatcaagac aatatgtgag gcgtgaaaag acgcctaaat ggttagaaga acggtcatat
     3301 gaaaagcaac ctcaaaaaga ttacgaccca caattagaga aagaacgaga ggatttttta
     3361 aaacaacttg aactgaattg ggaataacgc aacttttcgt tgcgagttgg ttaactaaaa
     3421 gtatcgtgac ttttcaatta ttttaattat agtttgagtt aaggagtgat tttatgaaat
     3481 tagctgaagc tataaaagaa cagagagaat taaaaggatg gtctcaagag gaattagcca
     3541 atatattaaa ggtttccaga caaagtgttt ctaaatggga aagtgccaaa aactatcctt
     3601 ccttagatat tttgattgct atgagcgatt tattcggaat ctctttagag catttaatta
     3661 aaggagatgc acgttttaaa aaagttattt tagagggaaa ttatagagag agtaacaggg
     3721 taccttccat agttgatttt ctttatgatt tttggtggct tttctttcct gttgccggat
     3781 ttttagtttg ggccattcat tcgttcatgg gttgatttta aaatcaaaat ttaatgcaca
     3841 tcaggcaata tgaaaaagga aagtattttt acgacacctt gaaatgtatt gataagaata
     3901 gaagagttta caaaaggcaa tatgaaatat atactaaaag taacaagaat ttcgtttgta
     3961 aacgcaccaa accccgaact attcgccgta gttcggggtt ttttggtgaa ttggctttaa
     4021 tcgtctatat tattttttat cacgtttatg gagctaataa ccaaataacg taacgagaca
     4081 accactgatt gccgttgtca tgatgtgaac aagaatttca ttcataaatc gcacctcctc
     4141 tctacgccga attgacatct gagagataga cgaccttcac attataacaa atctctcctt
     4201 ccatgaaata acatgaatat taataaatgt tgttatatat taaagataaa taaatataag
     4261 ggtgggattg ataatgtttg gcgatcaaag acaagaagct acaaaatatg tcattaaaga
     4321 aggttatcaa gatatttatt tcctcaacaa aaacggggaa tggtattatt tcgaagtaag
     4381 aagtgtctgg cgtggcaaac atattattag agttaaagat ggtttgttag gttggagaaa
     4441 agaaatagta actgaataaa gatacaaaat acttgccaat gcaaaagaat gaaaacgcgt
     4501 caaatcaacg atgtataaaa gatgcataaa agataatgaa agtaaaaagc cataaatgcc
     4561 gtcatatagg catttatggc tttttgtttg gttaatataa tcgatgatta tcagaagttc
     4621 aattcacata aaaaagcacg gctaatttat gaacttgtcg taccttttta attaaaatta
     4681 gttaccaaat agacgactcc aaaacccctt tttagcttcc gtttcttcac tcttttcttc
     4741 tatatcacta tcattgtcat ttggattagc tgaaacgtct tctctttgtg cttcttcacc
     4801 ttgtgtactt tcgtcgcttt gacttccaga tatatcctta tattgaatct ctgtttcttt
     4861 agtttcttcc tgatctcggt taatatttac aggactagta gtatatgttt tttcttgtgc
     4921 atcaacgttt tgtctagcgt tagtagatgt atcaaaggga taattaaggt gtttttcttt
     4981 ttctaattga ttttctaatt tttgaatctt tttattactc tctaatgcca gaacttgctg
     5041 attctcaagc aattttgatc tttcttgcaa ctcttttctg taaaattcaa ttgttgtttc
     5101 atgacgactt tcttgttttt caatttgatt atttaaagta ttaatctgat ttttaagtat
     5161 ttctactatt tgagcatcgc ttttagattc aaaattaata ttatccgact cttttttttg
     5221 tttgtcttca gtagcacttt caaaaccata tttcttttta ttttcattta ctctttgaat
     5281 gattttttct atatcggtat cttctttaat aaacgaagtg ttttcctgtt ttattgtctc
     5341 tatattcaat ctcttaatat tattaaaaat cgtttgctta ctaaccccta attcttcact
     5401 caaccttttt acacttttca tatcatcaac cctttataaa atgtttataa acccttagta
     5461 aaatattata agcttataac gattaataat aaagtaatcg acaaataaaa atataaattc
     5521 gccaaccttt ttatcaaggg tttgtaaagg gtttatggtg tttatcaaag gtttataaac
     5581 ctatattttc ccttttttaa gtttattcaa agtgctataa taaccttaat ataacaaaaa
     5641 ccccaactac tggtaatagt cggggtttgg taataaaacg tttagaaact gtttatttat
     5701 ataattttcg atacttagat tatataatga ataatatctt agtgcaagca cctagattat
     5761 taaataaaca caaaaaatgt aagcgtttta ttacctctta ccaaactaaa attcggaaag
     5821 aggtttttat tatgtcaatt aaactttaca acataaatga ggaatacaga gaaaagtttt
     5881 atcaaatacc gaaagtattt tttacaaatc ctaaatatat caagttatcc aatgatgcaa
     5941 aaatggcatg gggtattctt agagatcgtt tagatttatc aattaaaaat ggttgggttg
     6001 attctaaaac aggtaatatt tatttttatt acaaaaatga aaacccacaa aatatattaa
     6061 attgtaaaaa agataaagta attaaaataa agaaagaact taattgaagc cgaattacta
     6121 ctacaagaaa gacaaggttt aaataaaaca aataaacttt atttagttaa accagaagta
     6181 acagaagatg atatttataa aattcaaaag caagaaaaca ctgttgagag cagcaatggc
     6241 aaggaagtcg gaaaaaccga cttcaagaag tcgataaaac agaatctaat gatactgaac
     6301 ttagctatac tgaatctaac aatactgaaa taaatgatat gaatgacttg aatgatatag
     6361 agtccgaaaa tgaaattagt aatcattcaa gtcaatgcaa ggttatttaa tgcaaacaat
     6421 tttaactgaa tttgaagaaa cacatgcact ttatatgcgc cgtaaaaaca tgaaacaata
     6481 taatatcttt aatcaatcat tcaaatgatt ttataacaat caaagataca tcaaaatatt
     6541 tcgttaaatc atctgacttt ttaactgata gcgatgaaaa aatcaagaaa ttgtcagtga
     6601 tctagaaaaa gatttgtatc gaaaacgtat gttaagttat ggtggattgc ttaaataaaa
     6661 acataaaatt ttaaacttag acgatgctga agatggcaat ttaattaata caagtgatga
     6721 agataaaata gcaaatgaag aagaaaaggc acattcgatt acggcaattt tgaaaagcaa
     6781 aattattatt taaggcatta gtgataactg atgtcttttt ttattacttt tttgtaaaaa
     6841 aagtgctgtc ttatttttgt gacaaatgct gtatgtagtg tcacaaaagt gtgacacttg
     6901 agctttttat gaccccacac aaaaacattt tcgaagcctt gaaatatctg gctttgccag
     6961 atcccccatt cattgatggg gacaaatttc ccttatgctc ttacggagat tttagagtta
     7021 aattaaaatt tctaagtttt tgataagaaa agccctgcag gaatttagaa gaacgtgggg
     7081 aagttttaaa gaattttatg ctaattttta atttgcatgt aaatgggcag tgtctaaaaa
     7141 atagttattg tttttgctca aatttttgtt tttgaaaata gcatatattt atttggctca
     7201 tatttgcgtt ttaaaagcgt atataagttt tagataaaac tatatgaatc acccctaaat
     7261 ctttaaaatg ccctcttaaa attcaaaata agggcataaa aaataaaagc ccccccagat
     7321 aaagtgaggt agctttttct ttcgacgccg tatcgtcgat gtaattcaaa aaagttatgg
     7381 gctataaatc tacatcactt tttcaagaat gtagtagcaa tattcaacgc aaattaattt
     7441 ttataactgc tcggaatatc tcaagccgtt tctttaattt tgaaataaaa aaatcgacga
     7501 aggtcgatta cgtttttgca catgtcgatt tatccgacgt ataagtgcgc ccttacggga
     7561 tttaactaga ttatgacgac gaattttaga cctgtaaagc aataacaatt aggtctacca
     7621 ggtagataaa gtgtacaaag tagaaccgaa aaagttattt attttgaaga tttttcgtag
     7681 atataatttg ctaataaact tcctaaaact gaataaagaa aaatagttaa actcatgtaa
     7741 tcactccata attctgaagt gtctccatgt ctacgcatag acacgcattt atgccgagaa
     7801 aatttattgt gcgttgagaa gaacccttaa ctaaacttgc agacgaatgt cggcatagcg
     7861 tgagctatta agccgaccat tcgacaagtt ttgggattgt taagggttcc gaggctcaac
     7921 gtcaataaag caattggaat aaagcaccta tgattattta aaaatataag ttataataca
     7981 ttcaaaaata tttttgaatg aggtgtatta tgattcaaac ggttgttgct gctgctgttc
     8041 tttatattgc tacagcagtt gatttattag taattttatt aatatttttt gctagagcaa
     8101 agactagaaa agaatataga aatatttatg ttggtcaata tttagggtct attattttaa
     8161 tattagttag tttgttttta gcttttgtat taaattatgt tccagaaaag tggatattag
     8221 gtttattggg tttaatacca atttatcttg gaattaaagt ggctatttat gatgattgtg
     8281 aaggagaaaa gagagctaaa aaagaattga atgaaaaagg attgtctaaa ttagttggta
     8341 cggttgcaat tgttacgata gcaagttgtg gcgccgataa tattggttta tttgttcctt
     8401 attttgtgac attaagtgtt actaatttat tacttactct gtttgtcttt ttaattttaa
     8461 ttttcttctt ggtgtttact gcacaaaaat tagctaatat cccaggaatt ggagaaattg
     8521 ttgagaaatt tagtcgttgg attatggcta tcatttatat agctttaggt ttatttatta
     8581 ttattgaaaa tgacactatt caaacaattt taggatttat attttaattt agggtgtgat
     8641 tttatatgag ttatgaaaat acttgtgatg tgatctgtgt acatgaggat aaagttaaca
     8701 atgctttaag ttttttagaa gatgataaat ctaagaaatt acttaacatt ttagaaaaaa
     8761 tttgtgatga gaagaaattg aaaatcatat tatctttgat taaagaagat gagttgtgtg
     8821 tttgtgatat ttctttgata ttgaaaatga gtgttgcttc aacttcacat catttaaggc
     8881 ttttatataa aaatgaggta cttgattttt ataaagatgg aaagatggca tattatttta
     8941 ttaaagacga tgaaataaga gagtttttct ctaaaaatca ggagggtttt tgaaacgagt
     9001 gaaacgagtt tcttcttgtc ttgatactat aaaaaacaaa gttatataac ataagacgac
     9061 ttatcggaag ttatctatca tcgtatattc ggagatgata taatatacag gtcgcctatc
     9121 tctcaggcgt caattcagac gcagagagga ggtgcattac tttgctaatc attttcgttc
     9181 acatcatagc accagtcatc agtggctgtg ctgttgcgta ttatacttat tggcttagta
     9241 aacgcaacaa ataattaata ggcgacttta taagccagca tcgaaaaaat ccccacacta
     9301 ttgcagtagt gtggggattg gtgcattaat tgttaatcat tttcgttatc tagattataa
     9361 cactggttag tagaggaatg caaaaagata atattttaag tagtcttttt ggatattatc
     9421 atatgatata tttatgtaaa ttgatttttt attaaaggag catatatgag taatactaaa
     9481 attaaatatc aaatttttat ttcatcaact tatttagatt taatagaaga gcgtcaagca
     9541 gtagtagaag cagttttaaa cagtaataat attccagcag gtatggaact ttttaactca
     9601 agtagtaaaa gccaatggga aataataaca caatggatca atgaatcaga tgcagtgata
     9661 ttattgttag gttctagtta tggaacaatt gatagtgaag aaaatattag ctatacagaa
     9721 atggagtaca attttgctaa atctttaggt aaacctatat atgtttttga gatgaataaa
     9781 gcatctaccg aatccgcgga aaagtctaaa aaattagatg agtttaagac aaaagttaaa
     9841 gaaaatagac taattagtcc aaatattaat gatatttcgg aattaaaaag taaagtatct
     9901 cagactttaa tacaaagtgt acctagtgaa attaaagaaa atagtggatg gataagagta
     9961 caggattgtg gtaaaaatgt aaaaaaaata aaaaaattgg atgctataca aagagattta
    10021 gatacatatg attttttgaa agaagaactt tataaaatat tcatgtatgt aaaaactttt
    10081 tatttccagc acacaccatt tgatgatagt tatattgaag atatagaaaa ctacctgcta
    10141 aattatgaaa ataatccaca taaatcattc atgaaaataa aagtttatga ttataaaaac
    10201 tttttaaaac atttaaaaaa catattgatt ttaattaaac aaggaaaaaa gccattagct
    10261 caagccggtc attttaaatt aagcttttac gaagaggatt taaaattgtt acaaaaaaat
    10321 tataaggcta cgctagtcga tattaaagga ttgaataaac atttttataa ggaatgtgat
    10381 aggtgcttaa aagcatataa tatttttatt gagggtataa ataaacattt atatgaatga
    10441 tataagtaca ccctaatcat acatatataa ttgttgtcac tttactcgat atatgagaat
    10501 gactttacct aataaatgag aaaagcaacg cactatatta aaatgcgttg ctttttctat
    10561 tatcgaatac ctataactaa actatctcta gctgtttcta caacttctgt acttatttta
    10621 tctaaaccat ttatatccat aattctgtct atctgcgcaa ataaacgatg aatcagccta
    10681 aaattccctt ttgtaatttt aataatcgtc gttattgcct cataatcagt aaagtcttcg
    10741 agttttaaat caaatcctaa gtcttgacac ttatattcta atatatgatg cgtctcatct
    10801 ttacttagat tatcaaattc atgcgcaaaa cctatacgcg aatatagttg aggataacga
    10861 gatagttttt tctctatacc cggcatacca ataaatatca ttgctaaatt atgttcatca
    10921 taaatagctc ttaattgctc taaatgttgc actttaaggc gatcaatctc atctactata
    10981 attaaatcaa tatatttaaa catatcacta taatgtttac tatgttcatg tccatactta
    11041 tttacaacat gtaatttttg acccaaatca atgattgcag taatactata taaatcattc
    11101 cttaatttag tttgtttttc tgctggtgca ttataaaata ttttatccac cgatagtatg
    11161 tcatcggtcg ttttactagc taaatcttcc caacctctat ggttaacttg tttttcaata
    11221 gtatcccaat ttgtgtaata cctagacgat aaagtttttc cgacaccagg ttgaccataa
    11281 catataccaa tatactgata tttaatacaa gcatcacaaa attcagcaaa gcgtttatac
    11341 tctttcgttt caatgaaatt ttgattttta gtcattacga tatctcttta attttttgac
    11401 gtttttagtc gtttcttgag gtgaataacc acaatttttt tcttccttaa ttaattccgt
    11461 ggtacttgga ctagcaatat tttgttttaa gtgttttctt ctctgactac gagcgtgctg
    11521 tatgtctttt atatctatgg agtaatcagc tatttctgga catatagcag tacaaagaaa
    11581 ttcatctcta taaaacacac gtatttcagc catatcattt gggttataac gaatcagtac
    11641 atattcacct acataagctt ctagattagt attactgtat cgaaaacctt gaaaatgaat
    11701 gccatctgaa tgaacttttc tagatttagg tatttccaac aataataaat caagttgttc
    11761 taagctactt ggcatattag ggaaaaagtg attactattc caatgactga taggcgtggt
    11821 atgaatagta ctatgttctt tttgattata atcttcaatt aaaaagtaac gtaatttttc
    11881 ttcaaaattg tgaaaattta ttagattact aggagtatca ttattgttta tatatccagg
    11941 cagttgttct aagaaagttt gattaactgt ctgaaagaat cgttctattt ttccacgacc
    12001 acgaggaaca ccaactttcg aaaacatcaa gttaattttc aaatcaatag cgacttgttc
    12061 catatgatgt gaagtaaagt cacttccatg atctgtgtag aatttttcgg gtataccaca
    12121 cactggccaa ttggtatcat ttttattcca tattgcttgg tgtagtgtta atgctgtgtt
    12181 ttgagcattg gggggcatca aaacttaaga aatagcctgc aattgcacga ctatagtcat
    12241 ccataataat agttagccat ggtctattga tattaccttt ttggtctaat ataaagatat
    12301 ctagcaaagt atggtctgct tgccatattt cattgggtct cgaggattct ctaatttgta
    12361 ttaaatcgta cttattttga tagtattttt caccctgatg agagaaatca ataacagact
    12421 taggcatcgc tttaataaca ctataaattt gtttataact tggtatatca aaattatttt
    12481 ctttacagta attagtgatt ttcctatgaa ttgtggctat actatttctt ttattcataa
    12541 gcgcaaggcg ttcaatttca tcaattatct ttggttctat tttacgtgtt cctttatcag
    12601 ctcttgtcgc atagattagt cctttaagtc cttgttcttt atatgactta ttccacctat
    12661 aaagggtgct taacgcaatc cctttacttt tagaaatact tgataaagat tgttcgccta
    12721 ggataaaagg ttttataata ttgtactttt gagtagcttt tttacgtttg gcttcagaaa
    12781 aattagttag atacttctcc ttgtttttca tcttccttgt cctccaattt attgaggtaa
    12841 cgataaattg ttgtacgtga aacatgccac tgttctgcaa ttgtcttaat tggcgtgcca
    12901 ttatcataaa gggtcttaag taagtttaaa tctttttgat ttaatttttc tggtctgcct
    12961 ccatatcggc ctctagcacg tgctgcaatt cggccagctg aagaacgctc taaaattaaa
    13021 ttacgttcaa attctgcaaa tgctgcgaat aaatgaaata ataattgtcc agttgaactt
    13081 gatttatcca ttgttatatt ttcttctaaa ctatggaaac ttacacctcg ttcgttaagc
    13141 tcattaacta atgtgattaa atcctccata ttacgtccca atctatctaa tctccaaaca
    13201 acaatcgtat ctcctgacct cgcaaattca attgctttat ctaaaccagg tcttttactt
    13261 tttgaaccac tcatatggtc attaaatatc ttttcacaac catatgtatt taatcgatcc
    13321 tcctgtaaat ttaaattttg taatccagtt gaaactcttg cataacctat tttcaaaata
    13381 caaacctcct atatacatct tcagtgtacc ataacttaat tacaaggtct tttatgaaca
    13441 tagatttttt gttgggtttt tgaacacata atcctatgta attcttactt atttagacat
    13501 aaaagaagag tgtacagaaa atggttattt tctgtacact ctcatcttta ataatatatt
    13561 ttaaatgtca ttaacaaaac taatttaata agagtcaagc atagtttaca tatgtattat
    13621 gtttgaaaca tacctctaat tactttttac taatatcatt taaaatatct cgcaattctt
    13681 caatttcttt gttatttaat tcttcatttt tcgcaaaatt caacactaaa cttttcatat
    13741 cccctccata cagtttatta agaaaggttt tagcagtttt cattttaata tcgtcttctt
    13801 taatatttga tgagtaaaaa taaatatttt ctgatttgta tcgttttata atctcttttt
    13861 tatatagtct tgtgattaat gttctaatcg ttttatcact aacttcttta tatttttgaa
    13921 tttcaactac aatttcatta gctgatactg attttttatc ccatattata ttcataacat
    13981 cccattcagc catagatatt tcaacttgct tattggccat ttaaaacacc cattcctttt
    14041 aatatttttt cacttattaa ttcagcattt ttcccagacg ggtttccatc tgataaatgt
    14101 gtagcaaaat aatacttatc atgatttgta attacgtaac ctacaaacca cccattatta
    14161 tacttcccgt ttactatacc tgtacctgtt ttcccataca gttcatactt ttcatttttc
    14221 ttaatcaata atgaagaaga taattgattc tttgcttttt tactaaaatg gttattttgt
    14281 tccatcatat ttttaaaaac tattacttgt tcaagattag atattttcaa actatcttcc
    14341 atccaatagc ttttataact tcccaaattt tcattaccat aatttaattg cttgagttga
    14401 gccgcagtat agttctttgg tatttgatta ctaatacgtt cgaagtacca attaactgaa
    14461 ttttgcattg ctgtatttaa atcttgttcc ttattccaag actcgaaaag ataatgttta
    14521 tgattccatg acatacgtga atttttatca cttataatat gacggtcaag tccaaacagt
    14581 gctaaataaa ttttgtaagt tgaatcagga gaatatcttt ttctactttc tttttcatta
    14641 taaatatagt atttgtcttt cttcatgctg tacataacga aagatccact atttgaacca
    14701 aaatttttac tttcatctaa aatttggtaa tcgctttgta agggcttttt ataattataa
    14761 tcagttaagg attgccccat taaaaactgg ctttgaatta tcatgataaa aaaagtaaaa
    14821 atacaaataa aaattagaat taattttgac tgctttttca gatcggcttc ttttatatta
    14881 attaatctat ttttgagtaa tgatttttta ccattaaatg aatggcttaa tatatttttg
    14941 ttgaaaaatg aggtttttaa tacggagtcc ataatagatt ccgcataagt tttaaattca
    15001 tttttgttaa tattattaag gacaaatcta tcggcttcta cttcattgtc atgaattatc
    15061 tttcttttta caatatgtac taatggattg taactcataa taatagagaa gatattaaag
    15121 ataattaaat gtaaagtatc tctattttta gcatgagcat attcatgcag tattatatat
    15181 tttagtcttt tatcaattac acttttaaaa tatgaactag gaatcaaaat aatatatttc
    15241 ccataccaaa aagttattgg agattgaata gcctctgctt ttcgaatcac aatatttttt
    15301 ttatattgat ggttgaaaag tatcgtatct attttatttt tttcattttc gtttagataa
    15361 agtgactgtt tctttaaata tttaagatat aataaggctt tcaaaaattt aaaacttaaa
    15421 ataataacta aaactatcca aataactgtg cagatattat caattgaatc ccaattaaac
    15481 ttatggatat ctgttgcgaa ctcttgaaca ggtttggtgg taattatgtt atggttcaag
    15541 tcgtgtgact tactttcaac tgtgggcgct tgattattca cattattaaa ttttataaaa
    15601 gagaatttaa ttgggataaa aggaattaat ccagcaagca cagttagata ccaaacttta
    15661 taatttaaac tataattaaa atagcgtttt aatatgtacc taaaaaagac taacaataga
    15721 aatataaaac aaaagcttac tacactcatt attagtaatt tagccatttt gacaccttct
    15781 ttcaaatatt tataataaac aattgacatc aatattacaa ttgtaatatt attgttttgt
    15841 aaaaattaca actgtaatat cggagggttt attttgaaaa agttaatatt tttaattgta
    15901 atggctttag ttttaagtgc atgtaattca aacagttcac atgccaaaga gttaaatgat
    15961 ttagaaaaaa aatataatgc tcatattggt gtttatgctt tagatactaa aagtggtaag
    16021 gaagtaaaat ttaattcaga taagagattt gcctatgctt cgacttcaaa agcgataaat
    16081 agtgctattt tgttagaaca agtaccttat aataagttaa ataaaaaagt acatattaac
    16141 aaagatgata tagttgctta ttctcctatt ttagaaaaat atgtaggaaa agacatcact
    16201 ttaaaagaac ttattgaggc ttcaatgaca tatagtgata atacagcaaa caataaaatt
    16261 ataaaagaaa tcggtggaat caaaaaagtt aaacaacgtc taaaagaact aggagataaa
    16321 gtaacaaatc cagttagata tgagatagaa ttaaattact attcgccaaa gagcaaaaaa
    16381 gatacttcaa cgcctgctgc tttcggcaag actttaaata aacttatcgc aaatggaaaa
    16441 ttaagcaaaa aaaataaaaa tttcttactt gatttaatgt ttaataataa aaacggagac
    16501 actttaatta aagatggtgt tccaaaagac tataaggttg ctgataaaag tggtcaagca
    16561 ataacatatg cttctagaaa tgatgttgct tttgtttatc ctaagggcca atctgaacct
    16621 attgttttag tcatttttac gaataaagac aataaaagtg ataagccaaa tgataagttg
    16681 ataagtgaaa ccgccaagag tgtaatgaag gaattttaat attctaaatg cataataaat
    16741 actgataaca tcttatagtt tgtattatat tttctattat cgttgacatg tataattttg
    16801 atatcaaaac ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa
    16861 caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag tttaattata
    16921 ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt
    16981 tataaggtta aataattctc atatatcaag caaagtgaca taattgttga atttatagta
    17041 actgaaacgt atgattaggg tgtatattaa ttatgagatg gaaatcaatg attgttggct
    17101 atgcgagagt atcatcgaac gatcaaaatt tagaaagaca acttgaaaat ttaaagacgt
    17161 tttgtgctga aaaaatattt acagaaaaac agtctgggaa atctattgaa aatagaccag
    17221 tacttcaaga agcacttaac tttttgagga tgggagatcg gtttgtagta gaatccattg
    17281 attgtttagg tcgtaattat gatgaagtgg ttggcaatcc attactggat aagtttatga
    17341 aagatttaat tattcaaata ttagcaatgg tttcagaaca agagagaaat gaaagtaaac
    17401 gtcgacaagc gcaaggaatt caagttgcga aggaaaaagg cgtatataaa gggcgcccct
    17461 tactttattc accaaatgct aaagatccac agaaacgtat tatttatcat cgcgttgtag
    17521 aaatgttaga agaaggccaa tcaattagta aaattgcgaa agaagtaaat ataacaaggc
    17581 agacgattta cagaattaaa aacgataatg atttgattta ataaactttg tttcaatgca
    17641 actgagagct tctcacagcg tctcaaaaca gatgattata atctattatg agtttttaat
    17701 aataagacaa gaaaagagag cgtaaatatg ctctctcttt ttctcattaa cgaacgttta
    17761 taacgcaatc atgatgagag tattaataca gttaattatt tgaaatactt tagtttatat
    17821 aatgttgaat tataggtagt gaaattgtat ttattataca ttgtcatatc gttgtttaat
    17881 gatagttaat ttaaggataa tagaattaat attatatata gatgaacaaa acaagcaaca
    17941 gagcagaaaa aacactttta gattattttt actcattctc tattttattt atttttgcta
    18001 taataaataa ataatattaa atggaggtgt ttttatgaaa aagaaatttg tttctagttg
    18061 tattgctagt acaatcttat tcggcacttt attaggtgta acttataaag ctgaagctgc
    18121 aacagtacat gtagctggtg gtgtttggag ccatggtatt ggaaaacatt acgtatggtc
    18181 ttattatagt cacaataaaa gaaatcatgg ttcaacagca gtagggaaat attcatcttt
    18241 tagtggtgtt gctcgacctg gtgttcaatc taaggcatct gctccaaagg cttggggtgg
    18301 aaacaaaaca ttttatagtc ttcactaatt acatatatta attaaggaga atagacagaa
    18361 ttaagtaaac ttaattctgt ctattctctt gaaagaggca atatgaaaaa attcaaatta
    18421 tttttagatt ttagtacttt attattaatt tcagggctat tatttttatt tttcttcaaa
    18481 gaaaatgaag aaattatacc tgaaagttcc aatattttaa caatctctaa ttgggataaa
    18541 tctaacagta aatcaaaagt gttagatgtc attgaatcag gcgccaaaaa tcaaaatata
    18601 caaataatta aatccgttaa agattttgat aataaaaaag aattttttgt tttcaactca
    18661 aaacgaaata attctgactt tatacgtaat aaaacatcac ttttaactcc atcagattta
    18721 ttaaacagag agataaaagg aaagtattat attattggtg aacatttcaa tgtagaagag
    18781 cttacgagtg aattaaaaaa agctggttta acatctgaag ttgaatacat aaatagaaac
    18841 atattatttt ttgaattagt catagataat gatttattag tgcctttcat attcttaggc
    18901 gtattatatc ttttgtactt cttatatgac agaggatata atttgaaatt ttatgctatt
    18961 caaaagctac atggcttttc aactacacgc attatttttc ataatattca tataaaaatc
    19021 atttattggc tagtacttac cgctattttc tttattgcta atatattaat cattcatata
    19081 gccaatttag aattggattt cttaatgttt attcgtagat ttatcgtgtt gctttttgtt
    19141 tttgcttgta ttactacatt gctgtggctg atttcgtata gtcttctttt gaagttaagt
    19201 attccattaa tcttaaaagg gaaaaaagga tataagtttt caatatttat gggtaccttc
    19261 accaaatgta tcatggtgct aatactatct tttttactgg cacaaaattt aaatgcttat
    19321 acgaagttaa agaatatata tgactctgaa aaaatctggc aaaagttaga taattactat
    19381 acattagaga tttctcctaa tattagaaat tcagaagaaa aacaaatttt agaaactaat
    19441 atatacaatt taattaagaa aagcgaacaa cttggtggat tgcttttaaa gaataataat
    19501 atagccaatc ccgataaaaa caattacatt cctgaaaatg gcaatgtctt ttttatcaat
    19561 aacaattttc tgaatttcta taaaaatata aatcatgatt ttaaaatgat tgacaatcat
    19621 tcggataaaa taaacgtgtt tattcctcca aggttacaat atcaaaaatc tgaaattcaa
    19681 aaaaatcacc aaggatggat tgactttcaa aagcaacaaa acaaaaaagt agatgtgcaa
    19741 gtactgtcaa aaaacgtatc tgttttttct tttgatcgca caactaattt gaaatttggg
    19801 tatctagatt ctccaatagt aatgttgttg acacttgacg atttaacagg agatttttat
    19861 ttagctgctg tttctcaagg tggatatcta tttaaagatt tagatacact taaacagttg
    19921 cttaaagata atcacctaga agaagaaatt agcggtataa ctaattacaa ggattctgtg
    19981 ttaaatgaat taaatgaaac gaaaacaaaa atgataataa caatagttac tattattatt
    20041 aacgttttca ttcttttgat agctactgta tttgaaacaa ttcaatattt tagctggaat
    20101 aaaaagcaac tattattaag aaaaattcat ggttattcat tattcagcag taatgtacgt
    20161 tatttaacaa tttctatctt actttctatt atgttagctt acactacgca tattttattt
    20221 ggttcaaaga tattactatt cataataatg tctattgcta ttattcaaca tttacttcaa
    20281 attgcttata ttaaatatct tgaaaaatac tttaaagatt taatgaggga gatttaaaca
    20341 atgaaaagaa ctttaattat tgtaacaatt gtactattaa tcatcatact tagtctgtta
    20401 ttggttagaa atgagcattt agatcgtttt aacccactgt taaaagaaaa agtatcttat
    20461 gccaaagttc caaaagatac ccaggactat agaaatataa ctgtatactc taaaaatgga
    20521 gaaaaaaaga gttataaatt ggatttttta ggttatgatc ctacaaaaga atatgtgaaa
    20581 gtaaatcata aagggaaata tgtgcgttcg atagaataca ttacaaaaat attcctagct
    20641 ttttaaaatg ag
//
