LOCUS       NC_005910               5113 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Hafnia alvei plasmid pAlvA, complete sequence.
ACCESSION   NC_005910
VERSION     NC_005910.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224268
KEYWORDS    RefSeq.
SOURCE      Hafnia alvei
  ORGANISM  Hafnia alvei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Hafniaceae; Hafnia.
REFERENCE   1  (bases 1 to 5113)
  AUTHORS   Wertz,J.E. and Riley,M.A.
  TITLE     Chimeric nature of two plasmids of Hafnia alvei encoding the
            bacteriocins alveicins A and B
  JOURNAL   J Bacteriol 186 (6), 1598-1605 (2004)
   PUBMED   14996789
REFERENCE   2  (bases 1 to 5113)
  AUTHORS   Wertz,J.E. and Riley,M.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-APR-2003) Ecology and Evolutionary Biology, Yale
            University, P.O. Box 208106, New Haven, CT 06520-8106, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009946605.2-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 01:36:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7
            CDSs (total)                      :: 7
            Genes (coding)                    :: 6
            CDSs (with protein)               :: 6
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AY271828.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5113
                     /organism="Hafnia alvei"
                     /mol_type="genomic DNA"
                     /strain="MISC230"
                     /db_xref="taxon:569"
                     /plasmid="pAlvA"
     gene            complement(join(5012..5113,1..312))
                     /locus_tag="HS188_RS00005"
                     /old_locus_tag="pAlvA_1"
                     /old_locus_tag="ST569_p1"
     CDS             complement(join(5012..5113,1..312))
                     /locus_tag="HS188_RS00005"
                     /old_locus_tag="pAlvA_1"
                     /old_locus_tag="ST569_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_226229138.1"
                     /translation="MMKALNTVIAVAVFSFSLVGCSQHLSSAERHAKHFVYAEKSDFDP
                     SYQTRMQESARLSVPFFDQFYQQGKKDKLAGVSREQAQQQSDYFSSPAFLDAIQRESEF
                     AGKKYSQSQNQKWRLLMSQEAAGAYLDGYEGRK"
                     /name="Exc2 family lipoprotein"
     gene            491..1717
                     /locus_tag="HS188_RS00040"
     CDS             491..1717
                     /locus_tag="HS188_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846643.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_011178375.1"
                     /translation="MSGGDGKGHNSGAHDSGGSINGTSGKGGPSSGGASDNSGWSSENN
                     PWGGGNSGMIGGSQGGNGANHGGENTSSNYGKDVSRQIGDAIARKEGINPKIFTGYFIR
                     SDGYLIGITPLVSGDAFGVNLGLFNNNQNSSSENKGWNGRNGDGIKNSSQGGWKIKTNE
                     LTSNQVAAAKSVPEPKNSKYYKSMREASDEVINSNLNQGHGVGEAARAERDYREKVKNA
                     INDNSPNVLQDAIKFTADFYKEVFNAYGEKAEKLAKLLADQAKGKKIRNVEDALKSYEK
                     HKANINKKINAKDREAIAKALESMDVEKAAKNISKFSKGLGWVGPAIDITDWFTELYKA
                     VKTDNWRSLYVKTETIAVGLAATHVTALAFSAVLGGPIGILGYGLIMAGVGALVNETIV
                     DEANKVIGI"
                     /name="colicin-like pore-forming protein"
     gene            complement(1734..2042)
                     /locus_tag="HS188_RS00020"
                     /old_locus_tag="pAlvA_3"
                     /old_locus_tag="ST569_p3"
     CDS             complement(1734..2042)
                     /locus_tag="HS188_RS00020"
                     /old_locus_tag="pAlvA_3"
                     /old_locus_tag="ST569_p3"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="WP_008815957.1"
                     /translation="MFWGYFMAACILYASYGDDEPKIIALRIFGLASAILFPFSRFLIE
                     KAALRYTKKEFWKTGFFKDGVPKTYLMTLYFIFIFMTSIPIGVLSVCFEIKNVTAKI"
                     /name="colicin"
     gene            complement(2220..2477)
                     /locus_tag="HS188_RS00025"
                     /old_locus_tag="pAlvA_5"
                     /old_locus_tag="ST569_p6"
     CDS             complement(2220..2477)
                     /locus_tag="HS188_RS00025"
                     /old_locus_tag="pAlvA_5"
                     /old_locus_tag="ST569_p6"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_008815952.1"
                     /translation="MTALEQELLSAFEALQQSYVMQQQAWSESYSALQQMFTTTSAQAE
                     TLNAQNIRLSQSLSRLLEQVSDLSGQVQSLSRQVSSIKGR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2484..2948)
                     /locus_tag="HS188_RS00050"
     CDS             complement(2484..2948)
                     /locus_tag="HS188_RS00050"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_008815953.1"
                     /translation="MDKIFDLAKDFEQKSTKQASTIEANLAHVFKAHENAINTALHSSG
                     QRISSAIQEQSLTMRWMVLKSWLSIGVGLLVMLCLGGSVLWWQGTQIRENAQLMTEQSD
                     TLKKLNTKTWGIRFQEDTSGRFLVLPKGVKANPNWTFEDGTRQGVKLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2965..3636)
                     /locus_tag="HS188_RS00045"
                     /pseudo=""
     CDS             complement(<2965..3636)
                     /locus_tag="HS188_RS00045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3633..3956)
                     /locus_tag="HS188_RS00035"
                     /old_locus_tag="pAlvA_7"
                     /old_locus_tag="ST569_p7"
     CDS             complement(3633..3956)
                     /locus_tag="HS188_RS00035"
                     /old_locus_tag="pAlvA_7"
                     /old_locus_tag="ST569_p7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_011178376.1"
                     /translation="MSDKRTKMLTLWVTPEEHERLLARCDSPRLATWMREVCLEEKPTR
                     TSKPLPTLAPELLRQLAGMGNNLNQIARRLNSGEWSAHDRVQVVAALMALERELQLLRE
                     QAR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2186..3636)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaattcactt tcccgttgga tagcgtctag aaacgctggg ctagaaaaat aatccgattg
       61 ctgttgtgct tgctcgcgac ttactccagc gagcttatcc ttcttacctt gttggtaaaa
      121 ctggtcaaaa aagggcactg ataacctagc actctcctgc attcgggttt gataacttgg
      181 gtcaaaatca cttttttctg catatacgaa gtgtttcgca tgccgttctg cactcgacag
      241 atgttgagaa cagcccacca aactaaaaga aaaaacagcg acagctatca cagtattcaa
      301 tgctttcatc ataagcctct ttttaaaaaa aaaccatttt catcatagca ttagcgaagt
      361 aattattttg catacctacc gttattgact ttgttaaaag tcaggcataa gatcaaaata
      421 ctgtatatat aacaatgtat ttatatacag tattttatac tttttatcta acgtcagaga
      481 gggcaatatt atgagtggtg gagatggcaa gggtcacaat agtggagcac atgattccgg
      541 tggcagcatt aatggaactt ctgggaaagg tgggccatca agcggaggag catcagataa
      601 ttctgggtgg agttcggaaa ataacccgtg gggcggtggt aactcgggaa tgattggtgg
      661 cagtcaagga ggtaacggag ctaatcatgg tggcgaaaat acatcttcta actatgggaa
      721 agatgtatca cgccaaatcg gtgatgcgat agccagaaag gaaggcatca atccgaaaat
      781 attcactggg tactttatcc gttcagatgg atatttgatc ggaataacgc cacttgtcag
      841 tggtgatgcc tttggcgtta atcttggcct gttcaataac aatcaaaata gtagtagtga
      901 aaataaggga tggaatggaa ggaatggaga tggcattaaa aatagtagcc aaggtggatg
      961 gaagattaaa actaatgaac ttacttcaaa ccaagtagct gctgctaaat ccgttccaga
     1021 acctaaaaat agtaaatatt ataagtccat gagagaagct agcgatgagg ttattaattc
     1081 taatttaaac caagggcatg gagttggtga ggcagctaga gctgaaagag attacagaga
     1141 aaaagtaaag aacgcaatca atgataatag tcccaatgtg ctacaggatg ctattaaatt
     1201 tacagcagat ttttataagg aagtttttaa cgcttacgga gaaaaagccg aaaaactagc
     1261 caagttatta gctgatcaag ctaaaggtaa aaagatccgc aatgtagaag atgcattgaa
     1321 atcttatgaa aaacacaagg ctaacattaa caaaaaaatc aatgcgaaag atcgcgaagc
     1381 tatcgccaag gctttggagt ctatggatgt agaaaaagcc gcaaaaaata tatccaagtt
     1441 cagcaaagga ctaggttggg ttggcccagc tatcgatata actgattggt ttacagaatt
     1501 atacaaagca gtgaaaactg ataattggag atctctttat gttaaaactg aaactattgc
     1561 agtagggcta gctgcaaccc atgtcaccgc cttagcattc agtgctgtct tgggtgggcc
     1621 tataggtatt ttaggttatg gtttgattat ggctggggtt ggggcgttag ttaacgagac
     1681 aatagttgac gaggcaaata aggtcattgg gatttaactt gattgtatga ccgctatatt
     1741 ttagcggtca cattttttat ttcaaaacaa acagaaagaa caccaatagg aattgatgtc
     1801 ataaaaataa aaataaaata caaagtcatt aaatatgttt ttggcacacc atccttaaaa
     1861 aaacctgttt tccaaaattc ttttttcgta tatctaagcg ctgctttctc tattagaaac
     1921 cgagagaaag gaaatagaat agcgctagcc aaaccaaaga ttctgagcgc aattatttta
     1981 ggttcgtcat caccataact ggcgtaaaga atacaagcag ccataaagta tccccaaaac
     2041 atattatgta tgtaatattt ccttgtcata aaatccctca caaagtttta catttggtaa
     2101 aataacgttc tcaacaccgg agaaacgccc aattaaaagt ttcccatccc cactaacgac
     2161 gacgttccac tattcatctg attataattc tagcccctta aaccgcggac tatcacgcct
     2221 tatctgcctt tgatactcga cacttgcctg cttaagctct gcacctgacc actcaagtcg
     2281 cttacctgct cgagcaacct gctcagactc tgagacaagc ggatgttctg cgcgttcaag
     2341 gtttccgcct gtgcggaagt agtcgtgaac atctgctgca acgcgctgta gctttccgac
     2401 cacgcttgtt gctgcatcac gtaactctgc tgtaacgcct cgaaggcgct caataattcc
     2461 tgctctaatg ctgtcatctg tatttacttc ctctccaatt tgacaccctg ccgtgtcccg
     2521 tcctcgaacg tccagttcgg gttcgctttc acgcccttcg gtaacaccaa aaagcgaccg
     2581 ctcgtatcct cttgaaaccg aatgccccac gtcttcgtgt tcaacttttt caacgtatcc
     2641 gactgttctg tcatcaactg cgcgttctcc cgtatctgtg tcccttgcca ccacagcacg
     2701 ctcccgccca gacacaacat caccaacaga ccaaccccta tcgataacca gcttttcaac
     2761 accatccaac gcatcgttaa gctctgctct tggatagcgc tgctgattct ctgcccgctt
     2821 gagtgcaacg ccgtattgat agcgttctcg tgcgctttga acacgtgcgc gagattcgct
     2881 tcgatagtgc tcgcctgctt tgtcgatttc tgctcgaagt ccttcgctaa atcgaaaatc
     2941 ttgtccatag atagccccct ttaaccgaag attttgccca ccatctggcg acgcgagact
     3001 gatactgttt tttgcctgtc tgaccaccga aaaacccgca tctctaagcg cctcaacaac
     3061 atcagctcga ttttttattt ctcctgcatc aatacgtcgc agcaaaccat gtgtaatcgc
     3121 ctctgcggcc gcctgtttct ctcgaggcaa actgttcgcc gtcaccaacg cccgctgatt
     3181 gagcgggtca ttcgggtcat gtaatcctaa ttgcgcattc accaccgttt gccacgcatc
     3241 gatgcgcgga cggtcagcac ggtcaaagta cggttggaga cgcttaccgc gctgtaattc
     3301 gatgttcggt attacaaaat taagctccaa acgccctttg tctcgatgct ctacccacaa
     3361 caccgaatat tggtcagcat ccagaccagg caaaagcgca cgctcaaaac tgtccatcac
     3421 tttctgcttt gtctccgaag ccaaatcagc ctcagcaaac gacaacacgc cggaggtata
     3481 tttcttcgca tacgggctac tatcaatgag agctataacc tcctcagaag cccctctaag
     3541 caattttgca tcaactctag ccctatcctt acccagaagg taatcaaccg gtcctgagcc
     3601 ctttcctgtc cctctggcgt gaaattttac aatcatctcg cctgctcccg aagaagttgc
     3661 agctcgcgct ctaacgccat cagggcagcg accacctgaa cacggtcatg tgctgaccac
     3721 tcaccgctat tcagccgccg cgctatctgg tttagattgt tgcccatacc cgcaagctgt
     3781 cgcagcagtt ctggcgccaa cgtaggcaaa ggttttgatg ttcgggtggg tttttcctcg
     3841 aggcatacct ctctcatcca tgtcgccagt cgcggcgaat cacatcgagc aagtagacgc
     3901 tcgtgctcct ccggagtcac ccacagggtg agcattttcg tgcgtttgtc agacataacc
     3961 ctctccaaaa ccccgaaggg agacagcatt tatgctggct ttcttcgggg ttagacaagc
     4021 cgtaggcgcg tcagcgcggg tttcggggcg cagccctgaa ccagtcacgt agcgctagcg
     4081 tagtgtatac tggcttaata tgttagtgct gtgctgaatc tagcatagat tgcgcatgga
     4141 aataccgata aaagggaaca gagtgaccgt gcttaaatgg actgcgggcg atattccgct
     4201 tcctcgcaca gacagagctg tccggacaaa agccggacgt gcctagtctg cgcttgcggc
     4261 gagcggtgaa attattctca aacgtttaga gagtgaatat gtgaagaaag ataaaaagga
     4321 tgtcgccaaa aaggctcctc gccgccctcg tcctgtcttt tcggtgccct tcaagacagg
     4381 acgagccacc ccagacatca ctgtctaagg tactcggcct tgctccggac tatcggcgga
     4441 aaatgcatat tttcctgccc cttgctcgcg ctccggagac acaccctttc ctgctgtcac
     4501 atgctttggc cttggctgct gctgtaccgc cacgcttttt tttagtggtg ccgttgaatg
     4561 cccgttgtcc actggtgctg tgctgtacga catgcctgtc cgtacaaggt tttggtgcgc
     4621 gtcgcacgct cgggttgttt gagcaatacc cccgttcaac ccgactaccg cgctcatccg
     4681 gcaacatact cttgaggcca atccggatag aacacgaaaa aattcgccac tggcggtagc
     4741 cattggtaac tggaggtttg cgggggaggt gtaaatacac tataaataca tttgcacttg
     4801 aagtagtcgg cctctagagg ctaaactatc tgtacagatt tggtgactgt tcccgcaaaa
     4861 gtccagttac cgttcgaatc cgcgagttgc aagctcaacg aatccgaact aacccgcctt
     4921 tcggcgggtt ttttcgttta cgctcaggag actacgacga tctaaaaagg atctcaagcg
     4981 gatcttactc actaacaaca taatccccct cttattttcg tccctcatag ccatccaaat
     5041 aagcgccagc ggcttcttgg gacatcaata atcgccattt ctgattttgt gactgactat
     5101 actttttacc tgc
//
