LOCUS       NC_005324               6222 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli plasmid p9123, complete sequence.
ACCESSION   NC_005324
VERSION     NC_005324.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224315
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Enne,V.I., Bennett,P.M., Livermore,D.M. and Hall,L.M.
  TITLE     Enhancement of host fitness by the sul2-coding plasmid p9123 in the
            absence of selective pressure
  JOURNAL   J Antimicrob Chemother 53 (6), 958-963 (2004)
   PUBMED   15102746
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Enne,V.I., Bennett,P.M., Livermore,D.M. and Hall,L.M.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-AUG-2003) Department of Pathology and Microbiology,
            University of Bristol, Medical Sciences Building, University Walk,
            Bristol BS8 1TD, UK
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009946765.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 02:45:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AY360321.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
                     /plasmid="p9123"
                     /note="antibiotic resistance plasmid"
     gene            25..840
                     /gene="sul2"
                     /locus_tag="HS232_RS00005"
                     /old_locus_tag="p9123p1"
     ARG             26..837
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            901..1704
                     /gene="aph(3'')-Ib"
                     /locus_tag="HS232_RS00010"
                     /old_locus_tag="p9123p2"
     ARG             902..1701
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1704..2540
                     /locus_tag="HS232_RS00015"
                     /old_locus_tag="p9123p3"
     CDS             1704..2540
                     /locus_tag="HS232_RS00015"
                     /old_locus_tag="p9123p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<2647..3051)
                     /locus_tag="HS232_RS00020"
                     /old_locus_tag="p9123p4"
                     /pseudo=""
     CDS             complement(<2647..3051)
                     /locus_tag="HS232_RS00020"
                     /old_locus_tag="p9123p4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /name="transposase zinc-binding domain-containing protein"
     gene            complement(3095..3517)
                     /locus_tag="HS232_RS00025"
                     /old_locus_tag="p9123p5"
     CDS             complement(3095..3517)
                     /locus_tag="HS232_RS00025"
                     /old_locus_tag="p9123p5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_172684459.1"
                     /translation="MLFLKVKEDNFMSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGG
                     SFNGGGNGGHDNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARN
                     NMYLDTANNVRVGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            3702..4142
                     /locus_tag="HS232_RS00030"
                     /old_locus_tag="p9123p6"
     CDS             3702..4142
                     /locus_tag="HS232_RS00030"
                     /old_locus_tag="p9123p6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(4909..5058)
                     /locus_tag="HS232_RS00035"
     CDS             complement(4909..5058)
                     /locus_tag="HS232_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5629..5787
                     /locus_tag="HS232_RS00040"
     CDS             5629..5787
                     /locus_tag="HS232_RS00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     ARG             1705..2537
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(5094..5267)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          5191..5796
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
       61 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
      121 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
      181 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
      241 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
      301 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
      361 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
      421 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
      481 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
      541 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
      601 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
      661 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
      721 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
      781 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
      841 ctgcacattc gggatatttc tctatattcg cgcttcatca gaaaactgaa ggaacctcca
      901 ttgaatcgaa ctaatatttt ttttggtgaa tcgcattctg actggttgcc tgtcagaggc
      961 ggagaatctg gtgattttgt ttttcgacgt ggtgacgggc atgccttcgc gaaaatcgca
     1021 cctgcttccc gccgcggtga gctcgctgga gagcgtgacc gcctcatttg gctcaaaggt
     1081 cgaggtgtgg cttgccccga ggtcatcaac tggcaggagg aacaggaggg tgcatgcttg
     1141 gtgataacgg caattccggg agtaccggcg gctgatctgt ctggagcgga tttgctcaaa
     1201 gcgtggccgt caatggggca gcaacttggc gctgttcaca gcctatcggt tgatcaatgt
     1261 ccgtttgagc gcaggctgtc gcgaatgttc ggacgcgccg ttgatgtggt gtcccgcaat
     1321 gccgtcaatc ccgacttctt accggacgag gacaagagta cgccgcagct cgatcttttg
     1381 gctcgtgtcg aacgagagct accggtgcgg ctcgaccaag agcgcaccga tatggttgtt
     1441 tgccatggtg atccctgcat gccgaacttc atggtggacc ctaaaactct tcaatgcacg
     1501 ggtctgatcg accttgggcg gctcggaaca gcagatcgct atgccgattt ggcactcatg
     1561 attgctaacg ccgaagagaa ctgggcagcg ccagatgaag cagagcgcgc cttcgctgtc
     1621 ctattcaatg tattggggat cgaagccccc gaccgcgaac gccttgcctt ctatctgcga
     1681 ttggaccctc tgacttgggg ttgatgttca tgccgcctgt ttttcctgct cattggcacg
     1741 tttcgcaacc tgttctcatt gcggacacct tttccagcct cgtttggaaa gtttcattgc
     1801 cagacgggac tcctgcaatc gtcaagggat tgaaacctat agaagacatt gctgatgaac
     1861 tgcgcggggc cgactatctg gtatggcgca atgggagggg agcagtccgg ttgctcggtc
     1921 gtgagaacaa tctgatgttg ctcgaatatg ccggggagcg aatgctctct cacatcgttg
     1981 ccgagcacgg cgactaccag gcgaccgaaa ttgcagcgga actaatggcg aagctgtatg
     2041 ccgcatctga ggaacccctg ccttctgccc ttctcccgat ccgggatcgc tttgcagctt
     2101 tgtttcagcg ggcgcgcgat gatcaaaacg caggttgtca aactgactac gtccacgcgg
     2161 cgattatagc cgatcaaatg atgagcaatg cctcggaact gcgtgggcta catggcgatc
     2221 tgcatcatga aaacatcatg ttctccagtc gcggctggct ggtgatagat cccgtcggtc
     2281 tggtcggtga agtgggcttt ggcgccgcca atatgttcta cgatccggct gacagagacg
     2341 acctttgtct cgatcctaga cgcattgcac agatggcgga cgcattctct cgtgcgctgg
     2401 acgtcgatcc gcgtcgcctg ctcgaccagg cgtacgctta tgggtgcctt tccgcagctt
     2461 ggaacgcgga tggagaagag gagcaacgcg atctagctat cgcggccgcg atcaagcagg
     2521 tgcgacagac gtcatactag atatcaagcg acttctccta tcccctggga acacatcaat
     2581 ctcaccggag aatatcgctg gccaaagcct tagcgtagga ttccgcccct tcccgcaaac
     2641 gaccccaaac aggaaacgca gctgaaacgg gaagctcaac acccactgac gcatgggttg
     2701 ttcaggcagt acttcatcaa ccagcaaggc ggcactttcg gccatccgcc gcgccccaca
     2761 gctcgggcag aaaccgcgac gcttacagct gaaagcgacc aggtgctcgg cgtagcaaga
     2821 ctcgcagcga acccgtagaa agccatgctc cagccgcccg cattggagaa attcttcaaa
     2881 ttcccgttgc acatagcccg gcaattcctt tccctgctct gccataagcg cagcgaatgc
     2941 cgggtaatac tcgtcaacga tctgatagag aagggtttgc tcgggtcggt ggctctggta
     3001 acgaccagta tcccgatccc ggctggccgt cctgaccgcc acatgaggca tgttccgcgt
     3061 ccttgcaata ctgtgtttac atacagtcta tcgcttagcg gaaagttctt ttaccctcag
     3121 ccgaaatgcc tgccgttgct agacattgcc agccagtgcc cgtcactccc actcttacat
     3181 tgttcgcagt atctaggtac atgttattgc gtgcaccttc atgatgtata atgttattaa
     3241 tgacagcaga aagatcgaca gaattaatat tcttcgtacc tgggacagtt atcgggtgtc
     3301 ggccagtgat gagaccaaag gatgcagtat aagatgaacc actactatta tcatggccac
     3361 cattaccacc accgttaaaa ctacctcccg aacttccaga gccgctatga ccatcattgt
     3421 aggggtcatt tggtcccatg tgaccaaaac caccgacagt accgctattt aactctgtac
     3481 tcataaaatt atcctctttg acttttaaaa acagcaaaaa ctgtatttat aacaatgtgt
     3541 ttatatacag tattcgaaat actacgcccg tcaaaaccac accgtcaaca ggttttttgt
     3601 gtatttttca cgcctcacgg gcagcctcag accttttgtg ttaaaacagt aatatgttct
     3661 gctgttacaa tacggtacac acaggagaca ctgtcacttt tatggcatgg cacacagttc
     3721 aagagacact gaaattaaca ggaaagtcgc gctcgcaatt atatcgtgac atggctaaag
     3781 gccttgtcag ctatcgcaca ggaaaaaatg atcgccggga gttcgagacc agtgaactga
     3841 ttcgggccta tggcgagctg cgcacttctg agacaccaaa aaggcacaag gagggacact
     3901 ccaaatctgt cgttagccag gcggaaaatg ctcagctaga cgtcattcag cagaaactgg
     3961 acgctctgca gcagactgtt accctaatgc ttgaagacaa gatggcgcgg gaagcagaaa
     4021 accgcctgcg aaatgaggag agggaacaac tacaggccga aattagccgt ctggctgacg
     4081 cgctggcaca ggaaaaaaag cggggattct ggtcgcggct gtttcgctcc ggttcagagt
     4141 aacagtgcag gtgactctga tttaccataa catctgttat cagcaccgcc tgagaggccc
     4201 tcagggcgtt tctgacggga aaccggtaaa acaggcaaca aaccacccga aaacccgcca
     4261 gaaacgcgca ggctctgttt ttacggcatg catgagtatg cgcacaagtg catagcgcgg
     4321 aaattcacac cattcagccg gggtaagtcc tgtttccggc gttcttccca tccgaaaacg
     4381 gtgaacgggg acgcaattta ttgcgtggcc gtgaagcgtt gccggcgcag ccggaaaacc
     4441 cgctgtcagc acgttcagga agggggtgat gtacaaatgg cacagaggat gaaagtgacg
     4501 tatgagctga aggaatgatg taaaagtgat gtataagtga tgtaaaagtg atgtataagt
     4561 gatgtaaaag tgatgtatga actgaaggag cgatgtaatt ctgtagtcag gaagaataac
     4621 ccgttcatgc aggcatgaac gatacagtga ggaacgcttt ttttgtcacg atctcccctt
     4681 aacctgcgta aatcacccgc catggttgat tatttttcaa cctcaaaatg gtcatacacc
     4741 taacgcagcc gcgattttca tcgcaactgc atgaattaaa agaaaaaaaa ggaatatttc
     4801 gttttcacga gtaatacccg ggtattaaaa atccactgta attacatgaa aataaagaaa
     4861 taaaaggtaa taccggagta ttaccacgta cgcgggcaag cgaaaaagct ataacgcttc
     4921 ccggccgaca ggcgacccaa aatccacagg ggcatgcagg ttctcttcag taacctgcgc
     4981 cattaaatca aaaaggtcat attcgccggg aagctgcgta tcagtcgaat ccagttgttc
     5041 atcatattca gatggcatgg ggtgatatcc agtagctgag aggttgttag cgggtttcgg
     5101 ggcgcagccc tgaaccagtc atgtagcgct agcggagtgt atactggctt actatgttgc
     5161 cacgattgcg aaaatcagtg aagtgcttca tgtggcagga gaaaaaaggc agcaccggcg
     5221 cgtcagcaga atatgcgata caggagatat tccgcttcct cgctcactga ctcgctacgc
     5281 tcggtcgttc ggctgcggcg agcggtaccg gcttacggac ggggacggaa atttcctgga
     5341 agataccagg aagacactta gcaaagaaga gataaggcca cggcgaagcc gtttttccat
     5401 aggctccgcc cccctgacaa gcatcgcaaa atctgacgct caaatcagtg gtggcgaaac
     5461 ccgacaggac tataaagatc ccaggcgttt ccccctggta gctccctcgt gcgctctcct
     5521 gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcggtt tctcattcca
     5581 cgcctgacac tcagttccgt gaaggcagtt cgctccaagc tggactgtat gcacgaaccc
     5641 cccgttcagt ccgaccgctg cgccttgtcc ggtaactatc aacttgagtc caacccggaa
     5701 agacacgaca aaacgccact ggcagcagcc attagtaaca ggagtgcacg agaagataga
     5761 ttaagaggtc ttgaagtaga ggcctgactg cggctacact gaaagaacag ttttggtaat
     5821 ggcggtctcg tgaaccctgt taccgggtta agcagttccc caactgactt aaccttcgaa
     5881 caaaccgcct ccccaggcgg ttttttcgtt tacagagcaa gagattacga ttcattgaaa
     5941 aggatctcaa gaagatcctt tgtagattac tatcaattaa tcttccgaac atgacaccag
     6001 tcactgttga ttacggttaa accgctccgt aacaggtttc cctcgtattg gtgacttgca
     6061 gcaccaaaga agcaaaaaag ataaatatat ctacttattg agagatgata tatttatttc
     6121 caaaatgcta cagatacatc atcatgaaac ggatcaccgc aaacaggtta ctcgcctggg
     6181 gattcccttt cgacccgagc atccgtatga gactcatgct cg
//
