LOCUS       NC_005010               9328 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Enterococcus faecalis plasmid pEF1071, complete sequence.
ACCESSION   NC_005010
VERSION     NC_005010.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224704
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Balla,E. and Dicks,L.M.
  TITLE     Molecular analysis of the gene cluster involved in the production
            and secretion of enterocins 1071A and 1071B and of the genes
            responsible for the replication and transfer of plasmid pEF1071
  JOURNAL   Int J Food Microbiol 99 (1), 33-45 (2005)
   PUBMED   15718027
REFERENCE   2  (bases 1 to 9328)
  AUTHORS   Balla,E., Dicks,L.M., Du Toit,M., Van Der Merwe,M.J. and
            Holzapfel,W.H.
  TITLE     Characterization and cloning of the genes encoding enterocin 1071A
            and enterocin 1071B, two antimicrobial peptides produced by
            Enterococcus faecalis BFE 1071
  JOURNAL   Appl Environ Microbiol 66 (4), 1298-1304 (2000)
   PUBMED   10742203
REFERENCE   3  (bases 1 to 9328)
  AUTHORS   Balla,E. and Dicks,L.M.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-SEP-2002) Microbiology and Biotechnology, Szent Istvan
            University, Faculty of Food Science, Somloi ut 14-16, Budapest, Pest
            1118, Hungary
  REMARK    Sequence update by submitter
REFERENCE   4  (bases 1 to 9328)
  AUTHORS   Balla,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2001) Microbiology and Biotechnology, Szent Istvan
            University, Faculty of Food Science, Somloi ut 14-16, Budapest, Pest
            1118, Hungary
  REMARK    Sequence update by submitter
REFERENCE   5  (bases 1 to 9328)
  AUTHORS   Balla,E. and Dicks,L.M.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-1999) Microbiology, University of Stellenbosch,
            Victoria, Stellenbosch 7600, South Africa
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Name              :: GCF_009942225.2-RS_2024_06_25
            Annotation Date              :: 06/25/2024 01:25:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.7
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 9
            CDSs (total)                 :: 9
            Genes (coding)               :: 9
            CDSs (with protein)          :: 9
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AF164559.3.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="BFE 1071"
                     /db_xref="taxon:1351"
                     /plasmid="pEF1071"
     gene            complement(455..634)
                     /locus_tag="HKJ58_RS00005"
                     /old_locus_tag="pEF1071p2"
     CDS             complement(455..634)
                     /locus_tag="HKJ58_RS00005"
                     /old_locus_tag="pEF1071p2"
                     /inference="COORDINATES: protein motif:HMM:NF023063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin G-beta family bacteriocin"
                     /protein_id="WP_010773550.1"
                     /translation="MKNIKNASNIKVIEDNELKAITGGGPGKWLPWLQPAYDFVTGLAK
                     GIGKEGNKNKWKNV"
                     /name="lactococcin G-beta family bacteriocin"
     gene            complement(667..840)
                     /locus_tag="HKJ58_RS00010"
                     /old_locus_tag="pEF1071p3"
     CDS             complement(667..840)
                     /locus_tag="HKJ58_RS00010"
                     /old_locus_tag="pEF1071p3"
                     /inference="COORDINATES: protein motif:HMM:NF019739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin 1071A family bacteriocin"
                     /protein_id="WP_011117542.1"
                     /translation="MKQYKVLNEKEMKKPIGGESVFSKIGNAVGPAAYWILKGLGNMSD
                     VNQADRINRKKH"
                     /name="enterocin 1071A family bacteriocin"
     gene            1008..3158
                     /locus_tag="HKJ58_RS00015"
                     /old_locus_tag="pEF1071p4"
     CDS             1008..3158
                     /locus_tag="HKJ58_RS00015"
                     /old_locus_tag="pEF1071p4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393091.1"
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]; GO:0016020 - membrane
                     [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]; GO:0008234 - cysteine-type
                     peptidase activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_048949324.1"
                     /translation="MKKIIYQQDEKDCGVACIAMILKHYNTEIPIHKLRELSGTDLEGT
                     SAFGLKRTLEKIGFDSPAIQADNEVWKEKELPLPLIAHVLIDNKYMHYVVVYGIKGDTL
                     LIADPDPTVGKVKKSMEEFSKEWTGVLLLPTPKESYTPTKEKVAGLSSFLPIIWRQKGL
                     VFHIILASLFITLFGIGSSYYFQGILDYFIPNQARSTLNIVSIGLIAVYLFRVLFEYSR
                     SYLLVILGQRMSMAVMLQYFKHVLTLPMNFFATRKSGEIISRFLDANKIIDALASATLS
                     VFLDIGMVFLVGITLAIQNGTLFLITLASLPFYFVAILAFVKSYEKANQEEMAAGATLN
                     SSIIESLKGIETIKAYSGEEKVYNRVDREFIRLMKKSFRTVTLDNVQQGIKHAIQLISS
                     ALILWLGSFYVMDGTISLGQLITYNALLVFFTDPLQNIINLQVKMQTAQVANKRLNEIF
                     AIEPEQTNDSSEREIPNQTFQQEIRLENVSFSYNMKAPTLNNISCTIAPKSKIALVGVS
                     GSGKSTLAKLLVNFYAPSEGTIRYGKMNHLDIPFQQLREHVTYVPQESFFFSGTIVENL
                     TFGLQNMPSFERIIEVCEAVQLNTFIEQQPLRYDMVLEEGGANLSGGQRQRLAIARALL
                     KDADVLILDEATSGLDTLLEHAILDYLLTLEEKTLLFIAHHLPIAKACDHILVLHEGQL
                     VEQGAHDDLRYNQGVYQQLWDI"
                     /name="peptide cleavage/export ABC transporter"
     gene            3177..4544
                     /locus_tag="HKJ58_RS00020"
                     /old_locus_tag="pEF1071p5"
     CDS             3177..4544
                     /locus_tag="HKJ58_RS00020"
                     /old_locus_tag="pEF1071p5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_011117544.1"
                     /translation="MQQKDWLDHSSVYSQQHHTFYRWVLYPVILFLCLGALFLAFAKKE
                     VVIRTPAQLTAQKTEKLQVPVEAKIKTNTLKENQTVKKGEELVTFETTPLQNEKKQLEQ
                     ENTTIEEQKKAAQTFIDSLTNEQNLFETEDSFGYSNQVKSFLAEKEATRYASKQSETNT
                     QKEQEAYNKTKEQLEKQLTTRQKEQKEWEQVRTAWINEQSLEGFSTEITSRYQAWQSQL
                     TDVPEEQKKQIKATTLATIDEQISQVKKEIEQLQSEQSKLVAPTTSDNEVSSQTEKTKQ
                     TKELALATAKQKVVELTETQKKNETALKTINEQLAQSTLKAPITGTVHVNEEVKNQLEV
                     PKGTTLAEIYPKSKDPQLTFTALIPTSESMRIKPGMAIHFKLDKKGVATKTIDGTLTEI
                     SENSTTTEQGAFYTIKGTLKPPKEFTGRYGLTGELSLIVGKKTYWQQIKDMLLNQE"
                     /name="bacteriocin secretion accessory protein"
     gene            4565..4873
                     /locus_tag="HKJ58_RS00025"
     CDS             4565..4873
                     /locus_tag="HKJ58_RS00025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_168940485.1"
                     /translation="MNELEKLQIQEQKLPRKEKATTRKGISPFRTCLYKKAKVKSFDEA
                     QQAVTNYQPGTLGITNDQVEKLKQFANNLTWNESGNYWYTNEVKELTQFLAQFREEV"
                     /name="hypothetical protein"
     gene            5422..5859
                     /locus_tag="HKJ58_RS00030"
                     /old_locus_tag="pEF1071p6"
     CDS             5422..5859
                     /locus_tag="HKJ58_RS00030"
                     /old_locus_tag="pEF1071p6"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_168940486.1"
                     /translation="MTEQIGEQPTLRRKGANTMTEEKEQGVNRKSPKKRHYTQVNFRLT
                     EAEKERWQKQASSLNMTLPKFSKHLINLFFDSGTIKQPKIDKQQGTEIARHLAKLGGNV
                     NQIAKWCNTHQHDISDVQAKRLAENLAHVQKELKQVWQQLN"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5841..6881
                     /locus_tag="HKJ58_RS00035"
                     /old_locus_tag="pEF1071p7"
     CDS             5841..6881
                     /locus_tag="HKJ58_RS00035"
                     /old_locus_tag="pEF1071p7"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_010773542.1"
                     /translation="MATIKLARTTSCNRAISYAEKRAVEKDGVNCEITNAKQEMATIRQ
                     LFSKTDKTQAHLCIQSFSPQESQQLGPKKINQLGIELAEKMAPNHQVAVYTHADTDHIH
                     NHLVINAVNLETGNKYSHHNDFQRVSALHDQLLQEHQLQIVEKQAVERRTMAERKLAEK
                     GQWVWKDQIRQTIDALMKDSSISSYKAFREGLDQRGIKLHERGQNVVYELLEGNKRVRG
                     AKLGTDYEKDVIKHELDHREQTRTIETQARTLANGLRTSDFSRGSDEITHDPRRDSPID
                     FRMGTAISDLKHQLRQATETPSERTERKNRERVEALKQTDTRPIRHTHQPEPDLTPRQS
                     GPSLGL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6958..7281
                     /locus_tag="HKJ58_RS00040"
     CDS             6958..7281
                     /locus_tag="HKJ58_RS00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_141425320.1"
                     /translation="MSHLKELTANLEKAQEEVQEAMGMFARKQLAQAKTVNNIKDVAMS
                     AVFVLITLAVLWTVGYLLGTFFQLDEFYRWLVASTIGKGVLWGLGLALLVGSIGSVVYS
                     VKN"
                     /name="hypothetical protein"
     gene            7837..8820
                     /locus_tag="HKJ58_RS00045"
                     /old_locus_tag="pEF1071p9"
     CDS             7837..8820
                     /locus_tag="HKJ58_RS00045"
                     /old_locus_tag="pEF1071p9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_011117547.1"
                     /translation="MANELVKYHSELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKELSNYKATANMRFIDDLEETYKNLELRFGRRSQSGLTREFFVMFTKFKINGDT
                     DEPYVDIQIFEDALPLLNDLDSWVRYSLQQFNELQSSYSKTMFRLLKQFRTKGYAYFSK
                     EDFQELLDIPKSYKQGDIDRTVLQPIKQELTAIFKGLSIKKKYGRGRGKPVIGYQFTFK
                     PEIKNANDFNKHAPTKIEQSQALSQVNGQKNYEEQLTTLDDKIKEQERRLDLLKQLKEL
                     EQNKKDEKSLQIQTENKDRFTLSKRDKKDILSGLQISINYSKKHIV"
                     /name="replication initiation protein"
     BGC             1..8158
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7838..8820
                     /name="rep_cluster_1070"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5842..6878
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctagcaccac aggtttatta ttacgttaaa ggaaagagcc cataatatat taatttactt
       61 aattaaatag ttcagtacaa tgtatataca ttgagtaaaa aagaataaca caaacattgt
      121 ttgatttttt tttgttaaaa ctgatttctt ttttaaaatt atatataaat atataatagt
      181 aattatgctc aacaattcta acaaattagt taacagactt aaattgaaag gtactatcat
      241 agtaaaaacc attagaatga atacgctaaa gaaaaatgta taagcttttt ttattgcatg
      301 atctgaataa ttgatatcaa ttccaattaa aatgtataaa aatgttataa aattaaaaaa
      361 ggttatgatg cttataaaaa tatccatatt tatctcctta aaaataataa aaacatatgg
      421 cgttaacgcc atatgttttt attatactct ttaattatac attcttccac ttatttttat
      481 ttccttcttt tccaattcct tttgcaagac ctgtaacaaa atcatatgcg ggttgtagcc
      541 atggtaacca ttttccaggt cctccaccag taattgcttt caactcatta tcttcaatta
      601 cttttatatt acttgcattc ttaatgtttt tcatattagc atatctcctc tatttgattt
      661 cgattcttaa tgtttctttc tattaattct atcagcttgg tttacatcac tcatattacc
      721 taatcctttt aaaatccaat aagcagctgg acctacagca ttacctattt tactaaaaac
      781 cgactctccc ccaataggtt ttttcatttc tttttcattc aatactttat attgcttcat
      841 ttatcgttcc ccctaattat ttttgtaata taattctgga attatattac ttaatttatt
      901 aaatcaaagt ctttttatta tgtcaattaa ttatataaaa aggttttcaa aaatatttta
      961 ttatagtaat attgataaat gaataataac acaagtgagg agaaactttg aaaaagatta
     1021 tttaccaaca agacgaaaaa gattgtggcg tagcttgtat tgccatgatt ttaaagcatt
     1081 ataatacaga aatacccatt cacaagttac gtgagttgtc tggaacagat ttagagggaa
     1141 cttccgcttt tggtctaaaa agaactttag aaaaaattgg ttttgattct cctgctattc
     1201 aagctgataa tgaagtatgg aaagaaaaag aattaccgtt acctttaatt gcgcatgtct
     1261 taattgacaa taaatacatg cactatgtcg tggtttatgg aataaaagga gataccttat
     1321 taattgctga tccagatcca acagtaggaa aagtaaaaaa atcaatggaa gaattttcca
     1381 aagaatggac aggcgtttta ctgttaccta cacctaaaga gtcttatact cctaccaaag
     1441 aaaaagtcgc tggattgagc tcttttttac ccattatttg gcgtcaaaaa ggcttagttt
     1501 ttcacattat tttagcgtct ctttttatta cgctatttgg gattgggagc tcctactatt
     1561 ttcaaggaat tttagattac tttattccta atcaagcacg ttctacttta aacattgttt
     1621 cgattggctt gattgctgtc tatctgttcc gtgtcctgtt tgaatacagt cggagttatc
     1681 tcttagttat cctaggacaa cgcatgagca tggccgtaat gcttcaatac tttaaacatg
     1741 ttcttacttt acctatgaac ttttttgcta cccgtaaatc aggggagatc atttctcgtt
     1801 ttctagacgc aaataaaatc attgatgccc tcgccagtgc aaccttatcc gtctttttag
     1861 acattgggat ggtcttctta gtagggatta ccttagccat tcaaaatggt acattattct
     1921 taattacctt agcttcgtta ccgttttatt ttgtggccat tctagccttt gtcaaaagtt
     1981 atgaaaaagc caatcaagaa gaaatggcgg ctggcgctac cttaaattca agtattatcg
     2041 agagcctaaa aggaatcgag acgatcaagg cctacagtgg agaagaaaaa gtctataatc
     2101 gcgtggatcg agaatttatt cgattaatga aaaagtcctt ccggactgtt acgctagata
     2161 atgtgcaaca agggattaaa catgctattc agttaatcag tagtgccctt attttgtggc
     2221 taggttcttt ctatgtcatg gatggtacga ttagccttgg ccaattgatt acttacaatg
     2281 ccttgttggt gtttttcacg gatcctttac agaatattat taatctacaa gtcaaaatgc
     2341 aaacagcgca agtggccaac aaacgcttaa acgagatttt tgccattgaa ccagaacaaa
     2401 caaacgattc ttcggaacgc gaaattccaa atcaaacgtt ccaacaagaa ataagactag
     2461 aaaatgtctc cttctcctat aacatgaaag cacctacctt aaacaatatt tcttgcacca
     2521 ttgcaccgaa aagtaaaatc gccttggttg gggtaagtgg ttcagggaaa tcaacattag
     2581 caaaattatt agtgaacttt tatgcgcctt cagaaggaac cattcgctac ggaaaaatga
     2641 atcatttaga tattccgttt caacaattac gtgaacacgt cacctatgtg ccgcaagagt
     2701 cttttttctt tagcgggaca attgtagaaa acctaacgtt tggcttacag aacatgccta
     2761 gttttgaacg gattattgaa gtgtgtgaag ccgttcaact aaacacattc attgaacaac
     2821 agcctttacg ctatgacatg gtactagaag aaggaggggc gaatctttca ggaggacaac
     2881 ggcaacgatt ggcgattgct cgtgctcttt tgaaagacgc ggatgtttta atcttagacg
     2941 aagcaactag cggattagac acactattag aacatgcgat tcttgattat ttactaacgt
     3001 tagaagagaa aacattgctt tttattgcgc accatttgcc cattgctaaa gcgtgcgatc
     3061 acattctagt tcttcatgaa gggcaactgg ttgaacaagg ggctcatgac gacctccgtt
     3121 ataatcaggg ggtttatcaa cagttatggg atatttagga aaggacagaa aaattgatgc
     3181 aacaaaagga ttggctcgat cattcgagtg tttatagcca acagcaccac accttctatc
     3241 gatgggtctt gtatcctgtc attctttttt tgtgcttagg ggccttattt ttagcttttg
     3301 cgaaaaaaga agtcgtgatt cgaacacctg cacaactaac ggcacaaaag acagaaaaac
     3361 tacaagtacc ggtagaagca aagataaaaa caaatactct gaaagaaaac caaacagtaa
     3421 aaaagggcga agagttagtc acttttgaaa caaccccttt gcaaaacgaa aaaaaacaat
     3481 tagagcaaga aaacacaacg attgaggaac aaaaaaaagc ggcacaaact tttattgaca
     3541 gcctaacaaa tgaacaaaat ttatttgaga cagaagattc cttcggctat agcaatcaag
     3601 taaagagttt tctggctgaa aaagaggcaa cgcgctatgc ctcaaagcaa agtgaaacaa
     3661 acacacaaaa ggagcaagaa gcgtacaaca aaaccaaaga acaactagaa aaacaactaa
     3721 ctacgcgtca aaaagaacaa aaggaatggg agcaggtacg cacggcatgg atcaatgaac
     3781 aatccctaga gggattttca actgaaatca catccaggta tcaggcgtgg caatctcaat
     3841 taaccgacgt accagaagaa caaaagaaac aaattaaagc aacgacattg gctacaatcg
     3901 acgaacaaat cagtcaggta aaaaaagaaa tcgagcaact tcaaagcgaa caatcaaagc
     3961 tagtagcgcc aaccacgtct gataatgaag taagtagtca aacagaaaaa acaaagcaaa
     4021 ccaaagaact agccttggca acagcgaaac aaaaagtggt agaattaacc gagacccaaa
     4081 agaaaaatga gacagcactg aaaacaatca acgaacaact cgcacaaagt acgttgaaag
     4141 cacctatcac aggaactgtt cacgtaaatg aggaagtcaa aaaccaattg gaagtcccga
     4201 aaggcacaac gttggcagaa atttatccga aatcaaaaga cccacaactt acgtttaccg
     4261 cacttattcc tacgagtgaa agcatgagaa tcaaacctgg aatggcaata catttcaaac
     4321 tggataagaa aggagtagca actaaaacaa tcgacggaac gctaacggaa atttcagaaa
     4381 acagtacgac aactgaacaa ggagcttttt atacaataaa aggcacacta aaacctccaa
     4441 aagagtttac cggtcgctat ggtcttacag gggagctttc tctcattgtt ggaaagaaaa
     4501 cctattggca acaaatcaaa gatatgttat taaatcaaga atagaaaaag agtgagggat
     4561 tatcatgaat gagttagaaa aactacaaat ccaagaacaa aaactaccga gaaaagaaaa
     4621 agcaactact cgcaaaggaa tcagcccgtt taggacgtgc ctttacaaaa aagcgaaagt
     4681 gaaatccttt gacgaagccc aacaagccgt caccaattat caaccaggaa cactcggcat
     4741 aacaaacgat caagtagaaa aattaaaaca gtttgctaat aacctaacct ggaatgaatc
     4801 aggcaactat tggtatacga atgaggtcaa agaattaaca caatttttgg cacaattccg
     4861 cgaggaagtg taagtcgatt gaacgggttt agttgcttcg ataatccgaa gcaacttttc
     4921 ctccatttat ggaggaaaaa aggggcgttt tcgaacagaa aaccgaccct gaaaacgttg
     4981 gagaaaaatc gccgtacagg cgaccttttt caagcaacgt tcgggggatt gggggatacc
     5041 cccaacaagc tatttgtagt acgaagtgct agcaaataaa ctgcttgcca aaacctcagg
     5101 atgggcacat aatgaggttg cctgttttgt tggtaagtgg tgactgacta aggaacaacg
     5161 aacctacaaa acttagagga aaggtgtaac cgaatagggc acaacttgac gttttctctt
     5221 gtgagagttg tgagtgacaa acgaacattg gcactagaga aaggctaaag aaaattgacc
     5281 gaatagggaa tttttctggc gcgatctttt gatttttttt cgtagccgta ggcacgaaaa
     5341 cagacaaagg gaaacgactg aagaaggagt tttctctgtc tgcttggggt gagattgtcg
     5401 aacagacaac tcgcaaccca agtgacggaa caaatcggcg aacagccgac tttgagaagg
     5461 aaaggagcga acacaatgac agaagaaaaa gagcaaggag tgaaccggaa atcccccaaa
     5521 aagcgccact atacgcaagt gaattttcgt ttaactgaag ccgagaaaga acgctggcaa
     5581 aaacaagcca gtagcctgaa catgacctta ccaaagttct caaaacactt gattaattta
     5641 ttctttgatt ctgggacaat caagcaacca aaaattgaca aacaacaagg aacggaaatt
     5701 gcccgccact tagccaaact aggcggaaat gtgaatcaaa tcgccaagtg gtgcaatacc
     5761 catcaacacg acatttcaga cgttcaggct aaacggttag ccgagaactt agcacacgtg
     5821 caaaaggagt tgaaacaggt atggcaacaa ttaaactagc gcgtaccacc agttgcaatc
     5881 gggccattag ctatgcggaa aagcgcgccg tggaaaaaga cggcgtcaac tgcgaaatca
     5941 ccaacgccaa gcaagaaatg gccacgattc gccaactctt ctcaaaaacc gataaaacac
     6001 aagcccattt gtgtatccaa tcctttagcc cacaagagag ccaacaactt gggccaaaga
     6061 aaatcaatca gttaggcata gaattggccg agaaaatggc ccccaaccat caagtagcgg
     6121 tctataccca cgccgacaca gaccatatcc ataaccatct ggtgatcaat gcggtgaatt
     6181 tagaaaccgg caacaaatac agccaccata acgactttca gcgcgtatct gccctccatg
     6241 accagctatt gcaggaacac cagttacaga tcgtcgaaaa acaagcggtg gaacgtcgga
     6301 ccatggccga acgaaaactc gccgaaaaag ggcaatgggt ttggaaagac caaatcaggc
     6361 agacaatcga cgccttgatg aaggatagct ctataagctc atataaggcg tttagagagg
     6421 gtttggatca aagaggtata aaattacatg aacgaggtca aaacgtcgta tacgagcttt
     6481 tagaggggaa taagagggtt cgcggagcga agctaggaac agattatgaa aaggacgtga
     6541 ttaaacatga gttggaccac cgagaacaaa cacgaacaat tgaaacacaa gcaagaacgc
     6601 ttgcaaatgg acttagaacg agcgatttta gtcgaggaag tgacgaaatt acgcacgatc
     6661 caagaagaga ttccccaatt gatttcagaa tgggaacagc aatttcagac cttaagcacc
     6721 aacttcgaca agcaacagaa acaccatcag aacgaactga gcgaaaaaat cgagaacgag
     6781 ttgaagcact taaacagacg gatacaagac caattagaca cactcatcag ccagaaccag
     6841 atcttacacc acgacaatca ggtccttctc tcggactatg aaaactacct ggacaaacat
     6901 gtcaaagaaa tcaatcraga ataccgccta cttgtcgaac accaagccct tgcacaggtg
     6961 agccatttga aagaactgac agcaaacctg gaaaaagcac aagaagaagt gcaagaagcg
     7021 atgggtatgt tcgctagaaa acagttagcc caggcaaaaa cagtgaataa catcaaagac
     7081 gtggccatga gtgccgtttt tgtcttgatt acactcgctg ttctttggac agtgggctat
     7141 ctattaggca cgtttttcca gttggacgaa ttttatcggt ggttagttgc ctcaaccatt
     7201 ggcaaaggcg ttctctgggg gctagggctt gcactcctag ttggatcaat tggtagtgtg
     7261 gtttattcgg taaagaacta atggtctttc cagggtcgtt agggcttgcc ctgaccgtct
     7321 gaaagacgca aacaaggaaa actatcaagg aatgcttaac ctcgtcttag aaaggctgac
     7381 agtggcagtt aggacgatcc cacaggtctc cttgttgtgg gtttgctttt agcgagtgac
     7441 cgaaagggaa cgctgcaagt acaatgtggg agcaaagcga gcactccttt tagcgaacaa
     7501 cgtgagcgaa gcatttccag tttattgatt acgcgaacca gtttttagaa aaaagaggtc
     7561 ttttttaggc gggaattttt gcccccctct tttataaact ctttttaaac ctcttttaaa
     7621 cctctttacc gtcggctgta ctccacgccc tcaaaggagt ttatcgtttt atgaacgtag
     7681 ttttgtctgt ttatgaacgt agttttgtct gtttatgaac gtagttttgt ctgtttatga
     7741 acgtagtttt gtctgtttag aagtttcttt ataaacgtat tacgttttac aaatacgttt
     7801 caatatggta gtcttttctt caagaggtga ctatatatgg caaacgaact tgttaagtat
     7861 cattcagaat taaataccat tcctttgagg aaatttactc ctgtagaaat gaacttgttc
     7921 ttctctattg tttctagaat gcgtgaaaaa ggcgacaaga cagttcgttt tacttttgac
     7981 caattaaaag aattaagcaa ttataaagcg actgctaata tgcgtttcat tgatgattta
     8041 gaagaaacat ataaaaattt agagttgcga ttcggcagga gaagtcaaag tggcttaact
     8101 agagaatttt ttgtgatgtt tactaaattc aaaattaacg gtgatactga cgaaccttat
     8161 gtagatattc aaattttcga agatgcactt cctctgttaa atgacttaga ttcttgggtt
     8221 cgctactcat tacaacaatt caatgaacta caaagtagtt attctaaaac catgtttcgt
     8281 ttattgaaac aatttcgtac caaaggatat gcgtatttct ctaaagaaga ctttcaagaa
     8341 ttgttagata ttccaaaaag ctataaacaa ggagatattg atagaactgt tttacagcct
     8401 attaaacaag aacttactgc tatcttcaaa ggtttatcaa ttaaaaagaa atatggaaga
     8461 ggtcgcggta aacccgttat tggttatcaa ttcacgttta aacccgagat caaaaatgct
     8521 aatgatttca ataaacatgc ccctactaaa atcgaacaga gtcaggcgtt atctcaggta
     8581 aacgggcaaa aaaattatga agaacaacta acaactttgg acgataagat taaagaacaa
     8641 gagcgtagat tagacttatt aaaacaactc aaagaattag agcaaaataa aaaagacgag
     8701 aaaagcttgc aaatacagac cgagaataaa gatagattta ctctttcaaa aagggataaa
     8761 aaagatatac ttagcggttt acaaatttca ataaattata gtaagaagca catagtttag
     8821 cagcaatata aatatattaa accaactata tcaaatatct attgaatacc ttaaataaat
     8881 ttattttcaa tactttactt ttggagggat tgctatgtta tttttactaa aattttatta
     8941 cgctattaar cattatttct tatttatatc tatgtattat gtatatgaaa aatatggaca
     9001 aatataaatt attagcatta atacttatag ggggctgcat tttattatct atcgttctaa
     9061 gtatatttaa cttatcgttt atcttaatta gactgtctct attacttgga ttatctggcc
     9121 ttttactatt gcttaaacca gccattaaaa atacatttaa agatgatttt tcatttttag
     9181 aaggtatagc attaagtgct ataacatcaa gttacttagt aagcttaatt tcattattta
     9241 tacggtctta attattattg tgcgtttttc taaaaggtaa tacaaatata ataaacctgt
     9301 ggtgctagtt gattttaaga aaaatcaa
//
