LOCUS       NC_004335               8254 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Bacillus thuringiensis serovar thuringiensis plasmid pGI1, complete
            sequence.
ACCESSION   NC_004335
VERSION     NC_004335.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224822
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis serovar thuringiensis
  ORGANISM  Bacillus thuringiensis serovar thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Andrup,L., Jensen,G.B., Wilcks,A., Smidt,L., Hoflack,L. and
            Mahillon,J.
  TITLE     The patchwork nature of rolling-circle plasmids: comparison of six
            plasmids from two distinct Bacillus thuringiensis serotypes
  JOURNAL   Plasmid 49 (3), 205-232 (2003)
   PUBMED   12749835
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Mahillon,J. and Andrup,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-AUG-2002) National Institute of Occupational Health,
            Lersoe Parkall 105, Copenhagen DK-2100, Denmark
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009946295.2-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 02:46:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 11
            CDSs (total)                      :: 11
            Genes (coding)                    :: 10
            CDSs (with protein)               :: 10
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AY138809.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Bacillus thuringiensis serovar thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="H1.1"
                     /serovar="thuringiensis"
                     /db_xref="taxon:1432"
                     /plasmid="pGI1"
     gene            complement(join(8160..8254,1..307))
                     /locus_tag="HS166_RS00005"
                     /old_locus_tag="pGI1_p1"
     CDS             complement(join(8160..8254,1..307))
                     /locus_tag="HS166_RS00005"
                     /old_locus_tag="pGI1_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_000158318.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKMSEQEASD
                     FTVNVVEQRLSFEEILKVIKENSSKLPLN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(345..629)
                     /locus_tag="HS166_RS00010"
     CDS             complement(345..629)
                     /locus_tag="HS166_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_000126403.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEDLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            1095..1640
                     /locus_tag="HS166_RS00015"
     CDS             1095..1640
                     /locus_tag="HS166_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001000649.1"
                     /translation="MNDVLESSLLDATIDKLSIVADFKEGIKKEEFIRMVTSSNMPYSL
                     QANRSKNFGYEEVLKCDSVDCGYIELAGELKKASVDLPKLISHRIRLEIQIEQEKKARE
                     VGESLLSNAEYQGLFESLADVKELIAETDEDGRLTDERKLKRHERQIELTIEKLKGEIV
                     ALKSEIDEQELATGFKVV"
                     /name="hypothetical protein"
     gene            2011..3057
                     /locus_tag="HS166_RS00020"
                     /old_locus_tag="pGI1_p2"
     CDS             2011..3057
                     /locus_tag="HS166_RS00020"
                     /old_locus_tag="pGI1_p2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_000162903.1"
                     /translation="MTKKNQDEITGKLQPKKKQNIKIYGFIEEKMSESGRELFKSCSTF
                     NEFVATKDKQKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFNKLFKRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     NKKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREV
                     TDMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYG
                     GLLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELTESEKQEYNGH
                     LIDEMEVE"
                     /name="protein rep"
     gene            3060..3227
                     /locus_tag="HS166_RS00025"
     CDS             3060..3227
                     /locus_tag="HS166_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000276945.1"
                     /translation="MYKKGDIVKHTCWECYNEEMKIYRISKSKVKDGEDVYYSYKCWVK
                     CEKCGAVDDI"
                     /name="hypothetical protein"
     gene            complement(4088..4350)
                     /locus_tag="HS166_RS00030"
                     /pseudo=""
     CDS             complement(4088..4350)
                     /locus_tag="HS166_RS00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(4410..4667)
                     /locus_tag="HS166_RS00035"
     CDS             complement(4410..4667)
                     /locus_tag="HS166_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_236839308.1"
                     /translation="MEVIILAYVISKKVKGNIYFYGAQYVGTQPYYSKQYKYKCIYAIG
                     NQKIALERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKS"
                     /name="hypothetical protein"
     gene            5011..5238
                     /locus_tag="HS166_RS00040"
                     /old_locus_tag="pGI1_p3"
     CDS             5011..5238
                     /locus_tag="HS166_RS00040"
                     /old_locus_tag="pGI1_p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869766.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            5912..6076
                     /locus_tag="HS166_RS00045"
     CDS             5912..6076
                     /locus_tag="HS166_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000228871.1"
                     /translation="MVEALPVKQKNNYVCRGSSHSTTKKFPWIVGGVKKMASVGEDILQ
                     GSGDIMLSW"
                     /name="hypothetical protein"
     gene            6455..7684
                     /gene="mobV"
                     /locus_tag="HS166_RS00050"
                     /old_locus_tag="pGI1_p4"
     CDS             6455..7684
                     /gene="mobV"
                     /locus_tag="HS166_RS00050"
                     /old_locus_tag="pGI1_p4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_003275827.1"
                     /translation="MTEKGAMPMSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDII
                     QERTEQNYDLIHDKRKVDYKKIIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNFL
                     SPVEQRRFFEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFK
                     NKSELHDLQDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEKLEQEKKEID
                     SRLYDLKFSLNQAKSVDEIPVKEKGGFIRSKTVEIDSEDFESIKVLAKSSEVLRSENRR
                     LKNEKIKIEREKDDLYKGQRFLERQVTDLKRENRGLKEANDFLKKTLERVKEMYKEKLP
                     ELAGMIGYVKGSILDKMNRKFLKRHFAGDDEVRGAQKFLNHKQEHEEQQKRLKQVRRSQ
                     QKNRDQGLER"
                     /name="mobV"
     gene            7716..7997
                     /locus_tag="HS166_RS00055"
     CDS             7716..7997
                     /locus_tag="HS166_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002026684.1"
                     /translation="MDYFHGYGVKFIGTVKELAKKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNEQIMGLGEGLSAISERLRDMSEGIALISMDDKE"
                     /name="hypothetical protein"
     Typing          1875..3057
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.346"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6480..7681
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.761"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcttcttg ttcggacatt tttaattcat actcattgta taacagaaac atttctaggc
       61 aagctaatgc cgttcttttg tttgcattat agaatgcgtg gtttttagcc aatgattcaa
      121 ataatgcagt ggctttgtgg aagatagtcg gatatgcatc ctcttgtgct actgtttgtt
      181 gtggacggta tattgctgat tgtaataacc ctgtatcttt aacacctctt tgttcattag
      241 gtgaatacat gtcgataagt gtttcattga taaaaatagc ttgagtttct gttatataaa
      301 ttgtcatttc ggttttccta ctttcctcca cgcctgaagg ataattatct gtttaccaaa
      361 tctttaaatg caccgtcaaa tttattgatg gttctgttta acgattccaa gaacttagga
      421 ttgagatcct ctggcataga aatttttttg gattttttta taacgatttg ttcgttttct
      481 tcgttaattt ctaaacttac aacgtcacct tgtttgatat ttagagtagt gtaaaattct
      541 tgcggtaatg ttaaacctaa agaattgccg attttagtaa ctttacgttc gattttttgt
      601 gtttccatgt ggcgagtgtt tgctgtcatt tggacttcct ccttggaatt tgccacacta
      661 ttttttgtcg tagtctaatt gccactttgt gtttgtattg agtttttcga attatatagt
      721 gtgattttat gtttgttttg tttgtttgct tttggttttt tcggtcttct tagttttttc
      781 ttttgtttgt tgtgtttagt ctctctgtat tattatgacg tttagttaga atgttataac
      841 agttagaacg tattaaccgt taatacaatt atactcattt ttaaaaatat gtaaacaaat
      901 ttatatttca gtgcaaaaga tagggaagat tgtagagcct tgcttgaatt tgttttattt
      961 agataaagaa agtgtaaagg gagttacctg ttattctaat aagcaggtaa aggttgagaa
     1021 tttctctacc ttcttcagaa acatcttgga caagcacata aaaaatacaa ctgtttgggg
     1081 gttacggatt tgttatgaat gacgtactag aatccagttt attggatgca acgattgata
     1141 aactaagtat tgttgcagat tttaaagaag gtattaaaaa agaagagttt atacggatgg
     1201 ttacaagttc aaatatgcca tattcattgc aggcaaaccg ttcgaaaaat tttggatatg
     1261 aagaagtttt gaaatgtgat tctgttgatt gtggttatat tgagttagcg ggagaattga
     1321 aaaaagcgtc agttgatctt ccgaaattaa tttctcatag gattagatta gaaattcaaa
     1381 tagagcagga gaagaaagca agggaagttg gagaatcgtt actttctaat gctgagtatc
     1441 aaggtttgtt tgaaagtttg gctgatgtga aagaattgat agctgaaaca gatgaagatg
     1501 gtcgtttgac tgatgaacgg aaattgaaac ggcatgagag acaaatagaa cttactattg
     1561 agaagttgaa aggcgaaata gtcgcattga aatctgaaat agatgagcaa gaacttgcaa
     1621 cgggttttaa agttgtttaa aaacgcttag aagtgaaaat gaagaggctg agagatagga
     1681 ataatttttg tttttaaaag aaaaaatgcc cttgtccacg aagtggattg aagggcattt
     1741 ttttatggtt tttcggacac gaaaaatacg gttttcttct ttctttaata cataggtaac
     1801 tatttccgca cggaaatcga tgcctatgta ttgttttatc ccttgtgtac caaggttttg
     1861 caaaaaaaaa gacttgcttt tctaggcgga tagatttaat gttttaagtg actagcaaaa
     1921 aaccaaaatc tattcaagaa aggagcaagt ccgaaaaagg gattcgtata ccctttttta
     1981 tgcaattaat ataggactaa tatatcaaat atgacaaaga aaaatcaaga tgaaatcact
     2041 ggtaaattac aacctaaaaa gaaacaaaat ataaagattt atggatttat agaggaaaaa
     2101 atgagtgaga gtggtagaga attgtttaaa tcatgtagta cttttaatga atttgtggct
     2161 acaaaagata aacagaaaaa gaaacgagta aaggggaatg attgtaaaaa tcgtttttgc
     2221 ccgatctgtg cttggagaaa agcaggaaag gacgcggtga aaatcgcaac gatgatggaa
     2281 gcaatcaaga ttgaggaaaa gaaagagttt ctttttttaa cgttgacgac tccaaatatt
     2341 aaagctgata tggtaaaaag tgaaattgat agattcaata aagcttttaa taaattattt
     2401 aagagacgta atattcaacg ttctattaag gggtatatta gaaagctaga aatgacgtat
     2461 gataaagaac gatttatcac tgaagaaatg tataacaata ataaaaagag aaaagcatat
     2521 tatgattctc gtggcttgaa agtagacgat cataacccga attatgatac atataatcct
     2581 catttccatg tattgctttg tgttgataaa aattatttta aacgtaaaga gctttatatt
     2641 aagcaagaag aatggttaga aatgtggcga gaagtaacag atatgccaga gattacgcaa
     2701 gtacatattc aaaaagtaga gttaatacga gaaggaaatg cagttgcaga ggttgcgaag
     2761 tattcggcaa aagattatga aatgtcagtg agtcaagatg tgttcgacgt gttttattta
     2821 gctttaaaag gtcgacaatt aattgtgtat ggtggattgc taaaagatta cgctaaaaaa
     2881 tacgaagatg gcgaattgga caagtataag agtacagata aaaatgagta ttattataga
     2941 ttaatcgcaa tgtggaataa ggatttaatg aaatttgaac aagaatatca agagttaacg
     3001 gaaagtgaga aacaagaata taacggtcat ttgattgatg aaatggaggt tgaatagttt
     3061 tgtataaaaa aggcgatatt gttaaacata cctgttggga atgttataac gaagaaatga
     3121 aaatttatcg aatttcaaaa agtaaggtca aagatggtga agatgtatat tactcatata
     3181 aatgctgggt taaatgtgaa aaatgtggtg cggttgatga tatttgaatg acaaagagag
     3241 aaccaggctt aatattaagt ttggttttct ctttttttgt attgaaagaa aatcttttga
     3301 gtgcagtaag tagcattcaa aagttttttt atgatctaac tgagaactaa caagaaagat
     3361 tttttcttaa atttaaattt cgtgaatttg tgagtttatt tttaaagttt atttcgagat
     3421 atattttgtt ctgatgattt cgttttacta ctgtacgagg taaaaaccta aggtgtgcgc
     3481 atgaattttt ttagatatat ctatatcgat aaaataccgc acgtttttta atattttttt
     3541 gaaaataagt tttgggaaat tctaaatttt ttttgaggtt gattttggac ttttgatatg
     3601 agatttgatg ttgaaattta ttaaatttga aatctcgatt tttcgatttt tgaaattttt
     3661 gttttccgtt ttctctgtaa ttctgtactc caaaattacg gagtttatat agtacctcca
     3721 tgacgaactg aaaacgaaaa aactttgctc gcatattcaa tctatagata ctttgtatct
     3781 ttaaaaagtt gcaccgtttt aaatgagtta ttatagatcg tgtgatgaaa aaaagatgtt
     3841 tggattatat ttgaatttgt tatttccgtg aattttttat ttcgtgattc ctgtatattt
     3901 ttaatccttt ttactcttaa agtctgtttg ctttggttcg caaatgaaaa attcaaaaag
     3961 tcacgcataa cttttattaa atatataaat atattattaa aacacctaac ccttttgtga
     4021 tgaaaaaata taaatagtta aagaaaaaag actgacaaaa atatcagtct ttttttcttg
     4081 tttttagtta tagagagtat ctttttatgg tattttctac tttttctctc cagttttcta
     4141 tatcgcaatt gatattccaa tctttattaa ttcgcttgga atgaaactgt tatctaaaat
     4201 ccataaagtg agcctttcta atgtgattct ttcgtttcca atcgaataaa tacgttcact
     4261 ttgtttgcag gtatatggtt ttttaccaac atatttttct acataaaagt atctttttcc
     4321 gtttgctttg taatttgtta gaaatgccat gttaatgctc ctttcaggta tttttatggt
     4381 tagttaaaga gtagttctgt aatgtttttt caactttttt cataccaata tttcacatcg
     4441 tttatggata ctccgatttc tagtagttct tttggtatac ggttgttatc taatagccac
     4501 attgcgattc tttctaaagc tattttttga ttgccgattg cgtaaatgca tttatatttg
     4561 tattgctttg agtaatatgg ttgtgttcca acgtattgag ctccataaaa gtaaatgttt
     4621 ccttttactt tttttgatat tacatacgcc aaaataatga cctccaatta ttaaaattat
     4681 atatcacatt gattaatatt aactttatta ggttaatttt tcaacaaata aagtatgaaa
     4741 taatgcatat tttgaacatt tttatttaaa taggttttaa attagtttaa atgaacgcaa
     4801 taaataacct tatatgtata gaaattgatt ttcgtttgaa tgaagtatga aataatgcat
     4861 aatgtgattt tttattccat acaggtttaa aaatgtttta taaagtgcta aagttgtgtt
     4921 taaaaggtta aaaaacaact attatttgca caaaagtgcc tttataagaa aaatagtatg
     4981 tatatttcat gagaaaaagg agtataaaaa atgaaagtag taaataactt ccgtgtgatt
     5041 atggcggtaa agaatataga taatgttgca gagttggtta ggatgacagg ggtaagtcgt
     5101 aactctatca ataagttgtg gcgtaatgat aatgtgtcgt ctttaagaat agatacattg
     5161 ctaccgatct gtgaaaagtt aaatgttaaa ctatcggatt taattgaata tattccagat
     5221 gatagtgaga caaaataatt taggggctat tctatcttta gatatcttgt gtatgatgaa
     5281 atcggtatta atatttgcta gtggaaaaca ggttatattc gcatatggtt tttggtaggg
     5341 ttatatgcgt attttaaatt tcggcaaact tttatgactg tctttttctg tttgttatca
     5401 catgaatacg gtcaatgaaa ttataggctt gctaggtgtt gacgcatctt ggaagatgag
     5461 aggtaggcag atacttcgtg attttgttga atcttatgtg caatcatcgt aatgcagtaa
     5521 agtgaaatgg tggaattgat gagtattgaa aaaagagtcc ctatttatgg ggctcttttt
     5581 ttatttgtta ctaattactt ttaaaacgtc cacaaaacgt actacaaagg tctcaaagag
     5641 acctacaaat agatgttaaa aggactcaaa acgacctaca agggtgtttt ttgaatccca
     5701 gttagagagc tttagaaatt cagtaggcac agcctatttc accactttga actagaagtg
     5761 ggatcggatt tccttatgct cttttttatt ggaatgggtc taggaatgaa tatagaaata
     5821 gctgtagttc tattctatgg ctgttccgac agtgattctc ataaagaatg tgattgtatc
     5881 aatagattgg atgatgtttg aaaagtagag aatggttgaa gctcttcctg taaaacaaaa
     5941 aaataattat gtttgtagag gaagtagtca ttccactaca aaaaaattcc cctggatcgt
     6001 gggaggtgtg aaaaaaatgg cgagtgttgg cgaggatatt ttgcaaggca gcggagacat
     6061 tatgttgtca tggtgaatat aaaaagagac tttttaatat atggatgtta gcttttgaac
     6121 ggtggaggaa tacatacagt acctgtcaac ggtaagcgga cggaatgaag cgatagcgaa
     6181 atgatggagc attaaggagt tgataggtaa ctttgaccaa gaccctcttg ttttcccaaa
     6241 accatgtttt tgggatgcct accggcgagg gagtcctgag gggttgggga agttcgatga
     6301 aaaggggtca aggggaagag tttcccttgt gggtttgggc aaagcccatg attcttttgt
     6361 catgcctgtc atggctcggc ggtcgcccga aatgtcccgt agggcacacg tgtttacgca
     6421 gtaaactgta gtgtgctaca gtttacatgg aactatgacc gaaaaaggag cgatgcccat
     6481 gtcaaaattc atggtttgtc atatgcaaaa attcaagatg acagatgtga aaggattaca
     6541 aatacataat caacgtgaaa aagaaagtca ttcaaattca gatattattc aagaacgaac
     6601 ggaacagaat tatgatctta ttcatgacaa aagaaaagtg gattataaga aaataattca
     6661 aaatcgaatt gataatggtg ttgtatctaa tcgtgcgatt cgcaaagatg ctgttgtttg
     6721 ttgttcgttc atgatttcgg ctagtcctga ttatatgaat tttttatctc cagtggaaca
     6781 gagacgattc tttgaagagt ctgtttgttt tttgaaagca agatatggag aagagaactt
     6841 tgtgtatgct tctgtacatg tcgatgaaaa aacacctcat atgcatgtgg gtatggttcc
     6901 agttaatgaa aaacaaaaat tatcagcgta ttcatttttt aaaaacaaat cagaacttca
     6961 tgatctacag gataaaatct acgaacatgt gaaagaaaag ggatttgata tagagagggg
     7021 tgtttctagc gataggaaac atttatcgac gcagcggttt aaagcggtaa ctttgcaaca
     7081 agaaatagag aaattagagc aggaaaagaa agaaatagat agtcgtcttt atgatttaaa
     7141 attttcttta aatcaagcga aaagtgtaga tgagattcca gtcaaagaaa aaggcgggtt
     7201 tatacgctct aaaacggtcg agattgattc agaagacttc gagagtataa aagtattagc
     7261 taagtcttct gaagttctta gaagcgaaaa tagacgcctt aaaaatgaaa agattaagat
     7321 tgaacgtgaa aaagatgatt tatataaggg acaacgtttt ttagaaaggc aagtaacaga
     7381 cttgaaaaga gagaatcgag ggttgaagga agcaaatgat tttcttaaga aaacgttgga
     7441 gcgagtgaaa gagatgtata aggagaagct tcctgaactc gcagggatga ttggttatgt
     7501 taaggggagt atactggaca agatgaatcg caagttttta aaacgacatt ttgctggtga
     7561 tgatgaagta aggggtgctc aaaagttctt gaatcacaaa caagagcatg aagaacaaca
     7621 aaagagatta aaacaagtgc gaagaagcca acagaaaaat cgggaccagg gattggagag
     7681 atagtgtggg gacattattt aatcagcaac caagaatgga ttattttcat ggttatggag
     7741 ttaagttcat aggtactgta aaggaattgg ctaagaaaaa taatttaacg attgaagaga
     7801 cgtgtagaat tttagaattg actatgaaaa ttgatgatta tgatcgtaag gatgagcagt
     7861 tagcaggtat tggagaactt atcaaggaaa tgaatgaaca aattatgggg cttggtgaag
     7921 gtttgagtgc tataagtgaa cgattgcgtg atatgagtga aggtattgcg ttaatatcta
     7981 tggatgataa agaataatgg catgttagga ggctctgtgt gagtctcttc tctctttaga
     8041 cgtgtagcgt tagcgggaac ttgttttgca taatatagcg aggattgtag agccttgtat
     8101 aacatccttg aagatagttt gtttaataaa aaagacgtgg ttaaatccca cgtctttttt
     8161 taatttaaag gtaattttga tgagttttct tttataactt ttaagatttc ttcaaaagat
     8221 aatctctgtt ctactacgtt aacagtgaaa tctg
//
