LOCUS       NC_003457               5269 bp    DNA     circular CON 27-JAN-2024
DEFINITION  Salmonella enterica subsp. enterica serovar Enteritidis plasmid pC,
            complete sequence.
ACCESSION   NC_003457
VERSION     NC_003457.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224252
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Enteritidis (Salmonella
            enteritidis)
  ORGANISM  Salmonella enterica subsp. enterica serovar Enteritidis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Gregorova,D., Pravcova,M., Karpiskova,R. and Rychlik,I.
  TITLE     Plasmid pC present in Salmonella enterica serovar Enteritidis PT14b
            strains encodes a restriction modification system
  JOURNAL   FEMS Microbiol Lett 214 (2), 195-198 (2002)
   PUBMED   12351230
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   Rychlik,I., Gregorova,D., Doskocilova,J. and Sebkova,A.
  TITLE     Salmonella enterica serovar enteritidis serovar Enteritidis plasmid
            pC coding restriction modification system
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5269)
  AUTHORS   Rychlik,I., Gregorova,D., Doskocilova,J. and Sebkova,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-2002) Department of Bacteriology, Veterinary
            Research Institute, Hudcova 70, Brno 621 32, Czech Republic
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/27/2024 13:44:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8
            CDSs (total)                      :: 8
            Genes (coding)                    :: 7
            CDSs (with protein)               :: 7
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AY079201.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Salmonella enterica subsp. enterica serovar
                     Enteritidis"
                     /mol_type="genomic DNA"
                     /serovar="Enteritidis"
                     /sub_species="enterica"
                     /db_xref="taxon:149539"
                     /plasmid="pC"
                     /note="wild type ColE1 type plasmid; encodes a restriction
                     modification system"
     gene            complement(94..252)
                     /locus_tag="HS150_RS00045"
     CDS             complement(94..252)
                     /locus_tag="HS150_RS00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071791734.1"
                     /translation="MHEPPVQPDCCALPGNYRLESNPVRHALTPLAAAIGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(921..1112)
                     /locus_tag="HS150_RS00005"
                     /old_locus_tag="pC_p1"
     CDS             complement(921..1112)
                     /locus_tag="HS150_RS00005"
                     /old_locus_tag="pC_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1176..1499
                     /locus_tag="HS150_RS00010"
     CDS             1176..1499
                     /locus_tag="HS150_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956000.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1708..>2139
                     /locus_tag="HS150_RS00040"
                     /pseudo=""
     CDS             1708..>2139
                     /locus_tag="HS150_RS00040"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2177..2695
                     /locus_tag="HS150_RS00020"
     CDS             2177..2695
                     /locus_tag="HS150_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001749510.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            2702..2935
                     /locus_tag="HS150_RS00025"
     CDS             2702..2935
                     /locus_tag="HS150_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_181408656.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESSFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3335..3979
                     /locus_tag="HS150_RS00030"
                     /old_locus_tag="pC_p3"
     CDS             3335..3979
                     /locus_tag="HS150_RS00030"
                     /old_locus_tag="pC_p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529705.1"
                     /GO_function="GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eco29kI family restriction endonuclease"
                     /protein_id="WP_011017202.1"
                     /translation="MHNKKFDRSEHVYRNDSFLELIKDAVRFFSGTPVHSLPPPERFQG
                     AGVYALYYTGHYSLYDEYSRINRLAYNLPIYVGKAVPAGWRQSRISDHETRAGSELSNR
                     IREHSRNIAKTSNLNLCDFSCRFVIFEATGSDMISTVEAALIKIYKPLWNTVVDGFGNH
                     TPGAGRFAQAKSDWDVIHPGREWAEKCTGVHSEPYFIEERIKQYFSKSNFT"
                     /name="Eco29kI family restriction endonuclease"
     gene            3979..5127
                     /locus_tag="HS150_RS00035"
                     /old_locus_tag="pC_p4"
     CDS             3979..5127
                     /locus_tag="HS150_RS00035"
                     /old_locus_tag="pC_p4"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529706.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_011017203.1"
                     /translation="MRSLEIFSGAGGLAKGLELAGFQHVGFVELNKHACDSLRLNFDEE
                     KVFQGDIKNYDLSSIDKIDIVAGGPPCQPFSLGGKHKAHDDSRDMFPFAIKAIEVLQPK
                     AFIFENVKGLLRKSFADYFEYIILRLTYPCLSLSNVSDWRIHLDALRKARKDKHCGCTY
                     DVSFKLINAADYGVPQIRERVIIVGVRSDLNKKWSFPSVTHTQEKLLWEQHVTGDYWRK
                     HNIDAVIDKGISERLMKRFGIFPPSGKSWVTVRDALKNVPQPYESHGIGDHIFKDGARV
                     YPGHTGSYIDLPSKTLKAGAHGVPGGENMIRYEDGTVRYFTVYEGKLLQTFPREFLIAG
                     AWGEAMRQIGNAVPVLLAQKIGQQLYNLLNEEVEPLLCSNFN"
                     /name="DNA cytosine methyltransferase"
     Typing          751..836
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          complement(181..726)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.192"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1490..3039
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.374"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgccggaatc aagagctacc aactcttttt cccgaaggta actggcttgg tggagcgcag
       61 ataccaaata ctgtccttct agtgtagcct cagttaggcc accacttcaa gaactcttaa
      121 tatctcaatc caccttgtcc agttaccaat ggctgctgcc agtggcgtta aggcgtgtct
      181 taccgggttg gactcaagac gatagttacc gggcaaggcg cagcagtcgg gctgaacggg
      241 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gataccaaca
      301 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
      361 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
      421 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtctgtttt tgtgatgctc
      481 gtcagggggg cggagcctat ggaaaaacgc cagcggcgtg gccttttccg ggttcttgtt
      541 ttttgctcac atgtttcttt tggcgttatc ccctgattct gtggataacc gcattaccgt
      601 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag
      661 cgaggaagcg gaagagcgcc tgatgcggca ttttctcctt acgcatctgt gcggtatttc
      721 acaccgtata tggtgctctt ttcttattat ctgcactgat gccgcatggt taagccagta
      781 tacactccgc tatcgctacg tgactgggtc agggctgcgc cccgacaccc gctaaaacct
      841 gctgacgcgc cctgacgggc ttgtctgctc ccggcattcg ctcacagaca agctgtgacc
      901 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat ccccgaaacg tgcgaggcag
      961 ctgcggtaaa gctcatcggc gtggtcgtga agcgattcac aaatatcggc ctgttcatct
     1021 gcgtccagtt cgttgagctt ctccagcagc gttaatgtct ggcttctgat aaagcgggcc
     1081 atgttaaggg cggttttttc ctgtttagtc actgatgcct ccgtgtaagg gggatttctg
     1141 ttcatggggt aatgataccg atgaaacgcg agaggatgct cacaatacgg gttactgatg
     1201 atgaacatgc ccggttactt gaacgctgtg agggtaaaca actggcggta tggatgcggc
     1261 gggtctgcct gggtgagccg gttgcccgtt ccggaaaact gccgacactg gcaccgccgt
     1321 tactgcgtca gctggccgcc atcggaaata acctgaatca gacagcccgt aaggtgaaca
     1381 gcgggcagtg gtcttccggt gaccgggttc aggtggtggc cgcactgatg gccatcgggg
     1441 atgagctgcg ccggctgcgt ctggccgtca gggaacaggg gacgcgggat gatagttaag
     1501 tttcatgcca ggggaaaagg tggtggcagt ggtccggttg attacctgct gggaagggag
     1561 cgtaaccggg aaggggcaac ggtgcttcag ggtaatccgg aagaagtacg ggaactcatc
     1621 gatgccacgc catttgcgaa gaaatacacg tccggtgtgc tgtcgttcgc ggagaaggag
     1681 ctgccaccgg gaggacgtga aaaagtgatg gcgagctttg agcgtgtact gatgcccggt
     1741 ctcgaaaaaa atcagtacag catcctgtgg gtggagcacc aggacaaggg acggcttgag
     1801 ctgaattttg tcattccgaa catggagcta cagaccggaa aacgtctcca gccgtactac
     1861 gaccgcgcag acaggccgag aattgatgcc tggcagacgc tggtgaatca ccattacgga
     1921 ctgcatgacc ccaacgcccc ggagaaccgc cggacgctga cccttcctga taacctgccg
     1981 gaaacgaaac aggcacttgc tgagagcgtt acacgcggta tagacgcact ttaccatgcc
     2041 ggagagataa aaggccgtca ggatgtgatt caggcgctca cagaggcggg gcttgaagtg
     2101 gtcagggtga cgcgaagcag tatcagcatt gcagacccga acggcgggaa gaatatcagg
     2161 ctgaaaggag cattttatga gcaatctttt acagacgggc gcggagttcg agaaaaagct
     2221 gaaagagaga gccgaatcta cagagaaaat gctgaacgac gagttcagga agctcggcga
     2281 gtctgtcagc gaggctgtga cctcaaacga gacgaaaatc agagacgcta tagccctgtt
     2341 cacagcctcg acagagaaat cgctggaaaa acaccgggaa ggggtgaaag aggcgatgat
     2401 gcagcacagg aaggacgtgt taaagctggc agggaatacg ggcatgatgt tactggggat
     2461 agtctttctc ctgtttaccg cgagtggcgg gacgctctgg tatcttggcg gacggataca
     2521 gacgaacctg gaagaaatca ggaagcagga agagacattg cagaaactga acgcgaagac
     2581 atggggcgtg gagtttgtgc aggacgggaa caggaaattc cttgtccttc cgtacgggaa
     2641 atcagcggag gtgattccct ttcaggggaa agagtgggta catctgaagg agtgacacag
     2701 agtgacagag ctggaaatac atttgctgaa cgccttagag cagctgcaac aggactatat
     2761 gcagcggctg agcgaatggg agagctcctt cgtggaattg cagaagatgt tttcgcttac
     2821 gcaacgggac aacgcgatgc tgaacgagcg ggtcatgcag ttgagtcagc aggtgcagca
     2881 cttgagcgag cagacagaac gcttgagcca gttatacagc gagaactgga gataagagag
     2941 gaacggctga tacaggagcg cgaacatgcg ttatccctgg aacgggagcg tcagccggaa
     3001 atacaggaac gcacgctgga tggtccttcg ctgggatggt gaaaccatga aaaatggcag
     3061 cttcagtgga ttaagagggg ttaatgtggc ctgtaccatc tggttgcata ggtattcatg
     3121 cggttaaaat ttatcaggcg cgatcgcggc agtttttcgg gtggtttgtt gccattttta
     3181 cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg tgcgcgtaat gcgacgttat
     3241 ggtaaatgtg tatggttttg ggtatcagct ttatgatcgt atcataggtg atgttgagta
     3301 ggagtgttaa tataaaaacc tttcggaaaa cataatgcac aataagaaat ttgatagatc
     3361 tgagcatgtt tatagaaatg actctttcct agagttaata aaagatgccg ttcgtttttt
     3421 tagtggtacg cctgttcatt cattaccacc accggaacgg tttcaaggtg ctggggtgta
     3481 tgctctttac tatacagggc attattcatt atatgatgaa tactctcgta taaataggtt
     3541 ggcatataac cttcctattt atgttggcaa ggctgttcct gctggttggc gtcagtctag
     3601 aattagtgat catgaaacta gggctggttc tgaactatct aatagaatta gagaacatag
     3661 ccgaaatata gcaaaaacat cgaatctaaa tttgtgtgat ttttcatgtc gctttgttat
     3721 atttgaggct acggggtctg atatgattag tacagttgag gccgctctta ttaaaattta
     3781 taagcctttg tggaatacag ttgttgatgg ctttggaaat catacccctg gtgccgggcg
     3841 atttgcgcaa gctaaatctg attgggatgt aatacatcct ggcagggagt gggctgagaa
     3901 atgtacaggt gttcattctg agccatactt tattgaggag agaattaaac agtatttttc
     3961 taagagtaat tttacataat gagatcatta gagatttttt caggagccgg cggtttggct
     4021 aaaggtctag agttggcagg atttcagcat gtaggatttg ttgagttaaa caagcatgca
     4081 tgtgattctt tgcgacttaa ttttgatgag gagaaggtat ttcagggaga tattaaaaat
     4141 tatgaccttt catctataga taaaatagat attgttgctg gcgggcctcc ctgccaacca
     4201 ttttcattgg gcggaaaaca taaagctcat gatgactcta gggatatgtt cccatttgca
     4261 ataaaagcaa ttgaagtatt gcaaccaaaa gcttttattt ttgaaaatgt taaagggttg
     4321 cttagaaaat catttgcgga ttattttgaa tacataattc ttcgtttgac ttatccttgt
     4381 ttatcgttaa gtaatgtaag tgattggaga atacatctag atgcattaag aaaagctcgc
     4441 aaggataaac actgtgggtg tacatatgat gtcagtttta aattgatcaa tgcagctgat
     4501 tatggagttc cacaaattag ggaacgggtc ataattgttg gtgttcgaag tgatttgaat
     4561 aaaaaatggt cttttccaag cgtaacgcat actcaagaaa aattactgtg ggaacagcat
     4621 gttactggtg actattggcg aaagcataat attgatgcgg ttatagataa aggtatttct
     4681 gagcgtttaa tgaaacgttt tggtattttc cccccatcgg ggaaaagctg ggttactgtt
     4741 agggacgcat taaagaatgt tcctcaacca tatgagtcac atggtattgg agatcatatc
     4801 tttaaagatg gcgctcgagt ttatcctggt catactggaa gttatatcga tttgccttca
     4861 aaaacactta aggctggcgc tcatggcgta cctggaggtg aaaatatgat taggtatgag
     4921 gatgggactg ttcgttattt tacggtttat gaagggaagc ttcttcagac ttttcctcga
     4981 gagtttctga ttgcaggagc ttggggggag gctatgaggc agattggtaa tgcagttcct
     5041 gtgttgctag ctcagaagat aggacagcaa ttgtataact tattgaatga agaggttgag
     5101 cctttattgt gctctaactt caattaaaag tttgtgtctc tatggtggtg atacagccat
     5161 agaggcaatt tgcaaaaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5221 atctgctgcc tgtaaacaaa aaaaccaccg ctaccaacgg tggttcgtt
//
