LOCUS       NC_003124              11823 bp    DNA     circular CON 14-MAR-2024
DEFINITION  Aeromonas salmonicida plasmid pRAS3.2, complete sequence.
ACCESSION   NC_003124
VERSION     NC_003124.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224786
KEYWORDS    RefSeq.
SOURCE      Aeromonas salmonicida
  ORGANISM  Aeromonas salmonicida
            Bacteria; Pseudomonadota; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1  (bases 1 to 11823)
  AUTHORS   L'Abee-Lund,T.M. and Sorum,H.
  TITLE     A global non-conjugative Tet C plasmid, pRAS3, from Aeromonas
            salmonicida
  JOURNAL   Plasmid 47 (3), 172-181 (2002)
   PUBMED   12151232
REFERENCE   2  (bases 1 to 11823)
  AUTHORS   L'Abee-Lund,T.M. and Sorum,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUN-2001) Department of Pharmacology, Microbiology and
            Food Hygiene, Norwegian School of Veterinary Science, Ullevalsveien
            72, Oslo 0033, Norway
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009946025.1-RS_2024_03_14
            Annotation Date                   :: 03/14/2024 02:41:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 15
            CDSs (total)                      :: 15
            Genes (coding)                    :: 14
            CDSs (with protein)               :: 14
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AY043299.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11823
                     /organism="Aeromonas salmonicida"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:645"
                     /plasmid="pRAS3.2"
                     /note="atypical R plasmid"
     gene            167..523
                     /locus_tag="HS139_RS00005"
                     /old_locus_tag="pRAS32_01"
     CDS             167..523
                     /locus_tag="HS139_RS00005"
                     /old_locus_tag="pRAS32_01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873357.1"
                     /translation="MKYTTEQLEAIASKLREMPPVEKKKQEHSKQEAVRVLSKEIAALQ
                     KRGYTLDQISETLRGEGLSIATPTLKSYLQRAKPAKKAPVQAPGDTPPPRPADKKPADT
                     SKATFTPKPDSDDI"
                     /name="hypothetical protein"
     gene            536..1219
                     /locus_tag="HS139_RS00010"
                     /old_locus_tag="pRAS32_02"
     CDS             536..1219
                     /locus_tag="HS139_RS00010"
                     /old_locus_tag="pRAS32_02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mobD"
                     /protein_id="WP_009873358.1"
                     /translation="MAKSIYLIGGSKGGVGKSLVTMATVDYLQERGESVLLIESDTSNP
                     DVWKAYKESTETELINLDEADGWIQLVNLCDSKPDSVVVINTAARNNKGVSAYGETLNS
                     TLEELKRKLVTLWVINRQRDSLELLKEYMDAIPNADVHVVRNGHFGEEKKFELYNGSKL
                     RTAVEERGGKSVTFPDMADRVSDDIYSKRMSIAVALKELPIGNRAELTRWRNEAKKVLE
                     AVVHE"
                     /name="protein mobD"
     gene            1212..1850
                     /locus_tag="HS139_RS00015"
                     /old_locus_tag="pRAS32_03"
     CDS             1212..1850
                     /locus_tag="HS139_RS00015"
                     /old_locus_tag="pRAS32_03"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6753 family protein"
                     /protein_id="WP_009873359.1"
                     /translation="MSDLDDSFAKLLGRQPSDAERQSLYRVRDALGLKNNDALWLVLMA
                     LQHYQGQYEKFPQAIAQAAKDTLVNFKVTADATVKASAEAAKADLAQAVAAAAQEVAHN
                     TSAKQMWQWAAGCIAVAFLCVGLFGWYMHSSGKDSGYQAGYGAGYTEAKDEKAAAAWAN
                     TPEGRLAYRFAQTGSLASLAKCDRPGWYVEKGVCYVKPASDGTYGWRLP"
                     /name="DUF6753 family protein"
     gene            1835..2599
                     /locus_tag="HS139_RS00020"
     CDS             1835..2599
                     /locus_tag="HS139_RS00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OfxX fusion product"
                     /protein_id="WP_227015411.1"
                     /translation="MEVAMRIKIKGEITAERLAEALHAAAEKYEAVRPGHKVYGANLYL
                     TAFDADGLPFDLVDHRGEPLSITIEAKSGELVKPALTAEGEAHRQKAKEEARRQAEEAE
                     AEAQRRHRQTLDEYEQERQKRRKKEAEARKQFEDANAITAELLKTMPERFIDELNKTVQ
                     GVWDDLKPTETQGKKKGQPKALPVFSIHADGLVLSVETWKNPRRVLNPLCTLQHGEIAP
                     FWMHEAWLEAMRRIVDLLDTLTAAPAEALESQ"
                     /name="OfxX fusion product"
     gene            complement(<3239..3535)
                     /locus_tag="HS139_RS00025"
                     /pseudo=""
     CDS             complement(<3239..3535)
                     /locus_tag="HS139_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015958.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /name="resolvase"
     gene            complement(3743..4081)
                     /locus_tag="HS139_RS00035"
     CDS             complement(3743..4081)
                     /locus_tag="HS139_RS00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005291943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002310911.1"
                     /translation="MERVGMDCRRRPIPCLPPRVASRCMEPGHLDLNGSRRHLANGFTT
                     PRIGANQFLRRTVNAQTNPWQNISIASAISSSRTRRISGSVGSWPRVRMIVLLSLRTRL
                     GWRGCLTG"
                     /name="hypothetical protein"
     gene            complement(3985..5175)
                     /gene="tet(C)"
                     /locus_tag="HS139_RS00040"
                     /old_locus_tag="pRAS32_04"
     ARG             complement(3989..5175)
                     /name="tet(C)"
                     /gene="tet(C)"
                     /gene_name="tetracycline efflux MFS transporter Tet(C)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            5268..5948
                     /gene="tetR(C)"
                     /locus_tag="HS139_RS00045"
                     /old_locus_tag="pRAS32_05"
     CDS             5268..5948
                     /gene="tetR(C)"
                     /locus_tag="HS139_RS00045"
                     /old_locus_tag="pRAS32_05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(C)"
                     /protein_id="WP_010981365.1"
                     /translation="MNKLQREAVIRTALELLNDVGMEGLTTRRLAERLGVQQPALYWHF
                     KNKRALLDALAEAMLTINHTHSTPRDDDDWRSFLKGNACSFRRALLAYRDGARIHAGTR
                     SAAPQMEKADAQLRFLCDAGFSAGDATYALMAISYFTVGAVLEQQASEADAEERGEDQL
                     TTSASTMPARLQSAMKIVYEGGPDAAFERGLALIIGGLEQVRLSPASSPAGRTNLVLAL
                     AAGS"
                     /name="tetR(C)"
     gene            complement(5884..6876)
                     /gene="repC"
                     /locus_tag="HS139_RS00050"
     CDS             complement(5884..6876)
                     /gene="repC"
                     /locus_tag="HS139_RS00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_009873351.1"
                     /translation="MTQQRIKPAYAKFDPAHVFDGLFVPTKGKKRGRLYVPPRRFGDSE
                     ISFQGFEQLGADDQSILLAVSAQLGIDGLMIEAKPPGEIGQQLRLALDFDNDDGRPLKT
                     KKTSLRSLLIDAGYDDPESGAALARIKESLNRMGNAQIREINHATGWDYRCNLLSSRFN
                     HKTGEIFVAVNPRLTGAVFREQHVKVSLFERNELESEAAKLLHCWLCSNIRLGRSLGNG
                     NGAHIDTLAPHVWGKAAWEEASTKVRSVRRGQLRDALDEISDRTRGLQGGMGWAIDQTS
                     SGLVLVSRPKELPFLEGEHDMRPSEYHDLMSQPPMQEPDLFDPQDWTPD"
                     /name="repC"
     gene            complement(6857..7735)
                     /locus_tag="HS139_RS00055"
                     /old_locus_tag="pRAS32_06"
     CDS             complement(6857..7735)
                     /locus_tag="HS139_RS00055"
                     /old_locus_tag="pRAS32_06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_009873352.1"
                     /translation="MALDIMAAFTNEPPELDFIWPGFLAGTVGALVAPGATGKSFFALE
                     AAMSIACSVAGGDLVGLAPAHTGRVVYLAGEDPQPALVRRVHAIGQHLNQSAREAIAEN
                     LMLEPIMGKRLNVMDEAHLRRVIDYSAGARLIVLDTLSRIHSLDENSNGDMAHLVSVLE
                     HIAATTGAAVLYLHHVNKGSARDGQTDQQQAARGASALIDNARWCGYVAKMTEQEAERL
                     SDRGFDRAPIGNERRGLFVRFGVSKQNYDATPLDRWYQRHTGGVLLPVELQEIKRESGN
                     KGGKGYDSAAY"
                     /name="helicase RepA family protein"
     gene            complement(7764..8090)
                     /locus_tag="HS139_RS00060"
     CDS             complement(7764..8090)
                     /locus_tag="HS139_RS00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009873353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009873353.1"
                     /translation="MKTIYTIPAPDSLGAMIEVYGEPENAWYEWRIIDGGRTVRDTGTE
                     GHSAFQGRQYGQAEIALRDALMFASGLPIDGDTPTDAASLEAGYAAKAKAEAARQGAGT
                     PGSA"
                     /name="hypothetical protein"
     gene            complement(8087..8413)
                     /locus_tag="HS139_RS00065"
     CDS             complement(8087..8413)
                     /locus_tag="HS139_RS00065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019303585.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_009873354.1"
                     /translation="MMRGDLVTIAMQGDFGKPRPALVVQANQFSEHGSVTVLPVTSTLN
                     AAPLLRVTVQPSAENGLQKPSQVMVDKAMTVLRSKVGPAFGRIDADALLEVERCLAVFL
                     GIAK"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(8410..8634)
                     /locus_tag="HS139_RS00070"
     CDS             complement(8410..8634)
                     /locus_tag="HS139_RS00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin MazE family protein"
                     /protein_id="WP_043149934.1"
                     /translation="MATAHVNQRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQC
                     LLVAQADSADTAMQQFMDEALADVDGWTE"
                     /name="antitoxin MazE family protein"
     gene            complement(8701..11373)
                     /gene="traI"
                     /locus_tag="HS139_RS00075"
                     /old_locus_tag="pRAS32_07"
     CDS             complement(8701..11373)
                     /gene="traI"
                     /locus_tag="HS139_RS00075"
                     /old_locus_tag="pRAS32_07"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI/MobA(P) family conjugative relaxase"
                     /protein_id="WP_047353284.1"
                     /translation="MIVKKVPNPKKSASKAQRIGQLTSYVRSPESESPQEKCLYAGARG
                     FMMDDPKSQTAEMIALSQEAVRSKDTINHYVLSWREGEQPSPEQVEEAVSIFMDELGWK
                     DHQAIYGLHSDTDNIHLHIVINRVHPETLKIVEKNRGFDIELAHKAIARIEHAQGWQRE
                     QNGRYQVLENGELGRAPYDPEKPRQPDQKKRDMENRTGEKSAHRIAIEDGAAIIKQAQT
                     WEQLHRELAAKGMRYEKTGSGATVFVGDVGVKASDVDRNASLAKMQKRLGEYQPAPQRQ
                     QVAPREPEPIKPDVPGWKDYITGRKAHYAEKNADKLAQDKRQEQERKQLAEQQKARRDE
                     LMRGNWKGKGEVLNAMRSVIAAEQAAEKAALKEKHQKEREQHRQRFRPYPDLEQWQRMQ
                     KSPELAEQWRHRASEPQRIEGDRSEPPTPRDIRAYQPEIVGQQVHYSRKEEAGRGGGVS
                     FVDKGKSIDIHDWRNRDSTLAALQLSAQKWGSFTVMGNDEYKAMCGKLAAEHGFKITNP
                     ELQESIQQERQRIQQERVQAMKSEQLKQFERYAEAVGAERYRVTSIKMREDGSKQTFIL
                     DKKDGITRGFTPQEIEQRTPEMQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIR
                     DGYQPAAVLESSPGNYQAIITVPKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRA
                     PGYENRKPKHQREDGSYPEVRLLKAERRECIKALALSSQIDAEYQRQAALKAQQPERSK
                     AKPALELAAASGSAIDAYQRHYRDVLKRQRGGEVDLSRLDSMIAVRMRVTGHDQAAIEG
                     AIRQCAPATRQKDEGRDWNDYAQRTARYAYSAAGDRQAAELGKYRQQWEKLEGREPVRQ
                     QEQAKAQKIERDNSPGMSL"
                     /name="traI"
     gene            complement(11363..11674)
                     /locus_tag="HS139_RS00080"
     CDS             complement(11363..11674)
                     /locus_tag="HS139_RS00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014003869.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="WP_009873356.1"
                     /translation="MPFETQGPEPLDAVINVRLTAAEKARLKEDADLAGLSMSELVRRR
                     YFGRPIIANADAVMLKELRRIGGLLKHIHNESGGVYSKETAGALVALKDYFRKLNDDR"
                     /name="ribbon-helix-helix protein, CopG family"
     Typing          complement(6858..7735)
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8705..11373)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggattggcag gaacatgcgt agcatgtgta tgacaatccg catcctgtat tgctcaacca
       61 ctctactatc atatcagcag tttaggtgca cgggacgcac tttgcaacaa gcataaaagg
      121 cgctatgctg tcaactccgc aaccagtgag caaaggagtt agcagaatga aatacacaac
      181 agagcagcta gaggctatcg cttcaaagct tcgtgagatg ccgccagtcg aaaagaagaa
      241 gcaggagcac agcaagcaag aggccgtgcg cgtgctatcc aaagagattg ccgctttgca
      301 aaagcgtgga tacacgcttg accagatttc cgagacgctg cgcggcgaag gtctgagcat
      361 cgcaacccca acgctcaaga gctacttgca gagagcaaag ccagcgaaaa aagccccggt
      421 gcaagcaccg ggagacacac ccccgccccg ccccgccgat aagaagcctg cggatacatc
      481 gaaggcgaca ttcaccccga aacctgacag cgacgacatt taaggagtaa gcaacatggc
      541 gaaaagcatt tacctgatcg gtggcagcaa gggcggcgtt ggcaagtcgc ttgttacgat
      601 ggcaacggtt gattacctgc aagagcgcgg cgaaagcgtc ttgctgattg agtctgacac
      661 ctctaacccg gacgtatgga aagcctataa agaaagcacg gaaacggagc ttatcaatct
      721 ggatgaggcg gacggatgga ttcagctagt gaacctgtgc gatagcaagc cggatagcgt
      781 cgtggtaatc aacacggcag cacggaataa caagggcgtg agcgcctacg gtgaaacact
      841 gaatagcacg cttgaagaac tcaagcgcaa gctggtcacg ctgtgggtga ttaaccgcca
      901 acgggatagc ctcgaactgc taaaggaata catggacgca attccgaatg cggatgttca
      961 tgtggtacgc aatggacact tcggggaaga aaagaagttc gagctttaca acggctccaa
     1021 gctgcgcacg gcggtagagg aacgcggcgg gaaatcggtg acattcccgg acatggccga
     1081 ccgtgtgagc gatgacattt acagcaagcg catgagcatt gcggtagcac tcaaggaact
     1141 gcccattggc aaccgtgcgg agcttacgcg gtggcgcaac gaggccaaaa aggtattgga
     1201 ggccgtagtc catgagtgac ctagacgaca gctttgcaaa gctgctcggc aggcaaccta
     1261 gtgacgcgga gcggcaaagt ctttaccggg ttcgggatgc gctcggcctg aaaaacaatg
     1321 atgcgctgtg gctggtgctc atggccttgc aacactacca aggccagtat gagaagttcc
     1381 cgcaagcgat agcacaagcg gccaaagata ccttggtcaa tttcaaggta acggcggatg
     1441 caaccgtgaa agcatcagcg gaagcagcca aggcggattt ggcgcaagcg gtagcagcag
     1501 cagcgcaaga ggtcgcgcac aacacatcgg caaagcagat gtggcagtgg gcggcgggct
     1561 gcatcgcggt ggccttcctg tgcgtcggcc tgttcggctg gtacatgcac agcagcggca
     1621 aggattcggg ctatcaggcc ggatacggcg cgggatacac cgaagccaag gacgagaaag
     1681 cggcggcggc atgggccaac acgccagagg ggcgcttggc ctaccgcttc gcgcaaacgg
     1741 gaagcctcgc cagcttggcg aagtgcgata ggccggggtg gtacgtcgaa aaaggcgtgt
     1801 gctacgtcaa gcccgcatcg gatggcacct acggatggag gttgccatga gaatcaagat
     1861 caaaggagaa atcacggcag aacgcctagc ggaagcactg cacgcagcag cagaaaaata
     1921 cgaagctgtg aggccggggc acaaggtcta tggggccaat ctctacctaa ccgccttcga
     1981 tgctgatggt ctgccgttcg atctggtaga ccatcgcggc gaaccgcttt cgatcaccat
     2041 cgaggccaag tctggcgaac tggtaaaacc agcactcaca gcagagggtg aagcacaccg
     2101 acagaaagcc aaagaggaag cacgaaggca agctgaagaa gcggaagctg aagcacagcg
     2161 cagacacagg caaaccttag acgaatacga acaggagcgg caaaaacgac ggaaaaagga
     2221 agccgaagca agaaagcaat ttgaggacgc gaacgcaatc actgccgaac tcttgaaaac
     2281 catgccggaa cgcttcatcg acgaactgaa caaaaccgtg caaggcgttt gggacgacct
     2341 caagccaacc gaaacgcaag gcaagaagaa gggccagccg aaagcgctac cagtcttttc
     2401 catccatgcg gacgggctgg tgctttccgt cgagacatgg aagaacccgc gccgggttct
     2461 caatccgctt tgcacactcc agcacggcga aatagcccca ttctggatgc acgaagcgtg
     2521 gttagaagcc atgaggcgga tagtcgatct gctggatacg ctcaccgcag ccccggcaga
     2581 ggccctagaa agccagtaga ggcgttttcg cctgcaagga agggggtagc ggcggggcgg
     2641 gggcgggggt gtgtctgcaa gggctttagc ccgcgcaggg cgaagcccga aggcaaggct
     2701 tcagacgacg cctaaagcgg cggctgaaag ccgcgcatgg tagaagttct ttagaacttc
     2761 ttagaccgga attttggaaa aacgcgaaaa ctcagtactg acaagggttt gcgggcgttt
     2821 ttgtggcttg tcaggttacc acctgcgccg ggggtggtta ccacctgcgc cgggggtggt
     2881 taccacctgc gccgggggct tgaattaaat tagataaatc tcttattgat agacaaacac
     2941 actacactta atacccatta tgttaaaaac gacaggattc atgaaaatca tcagaattgt
     3001 gcaggatggt tcacctatcg attagatatg gggtctgaag gccaatagaa cgaaaacgta
     3061 cgttagtgaa gtaactgtct gatatatcga aacataatgt acattggaaa acgccatcaa
     3121 aacggtgtct ttttaatcga aaattggcac ttaacggact ttcttgtcta ctaatcgatt
     3181 tttcagacga taattttcct cttaccgtac gttttcgtac ggttggacct caaacccctt
     3241 tgagcgtggc aagctgcaca aagggatagg gcggcaccag agaaaaatca ctcagggtca
     3301 atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc
     3361 gatgcagatc cggaacataa tggtgcaggg cgctgacttc cggtgccagc agatattttg
     3421 gcagtttgcc ttggatcaga gccatctgac gcagggctag tgcagccgga tagtcaatag
     3481 ctaccggcag cggtgcggac tgttgtaact cagaataaga aatgaggccg ctcatggcgt
     3541 tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt tgcgggcaac
     3601 ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga
     3661 ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct
     3721 cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc
     3781 tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc
     3841 gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt
     3901 gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt
     3961 tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg
     4021 caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca
     4081 tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga tcgaagttag
     4141 gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct
     4201 ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat
     4261 ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc
     4321 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     4381 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     4441 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     4501 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     4561 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt
     4621 atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc
     4681 cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc
     4741 caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc
     4801 atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc
     4861 cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg gtcgccatga tcgcgtagtc
     4921 gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag
     4981 tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc
     5041 atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg
     5101 cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc
     5161 attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc
     5221 gcattaaagc ttatcgatga taagctgtca agcatgagaa ttcgcgaatg aacaagctcc
     5281 aacgcgaggc cgtgatccga accgcgctcg aactgcttaa cgacgtgggc atggaaggtc
     5341 taacgacgcg ccgactggct gagcgcctcg gggtgcaaca gccagcgctc tactggcatt
     5401 tcaagaacaa gcgtgcgttg ctcgacgcac ttgccgaagc catgctgacg ataaatcaca
     5461 cgcattcgac gccaagggat gacgacgact ggcgttcgtt cctgaagggc aatgcatgca
     5521 gttttcgacg ggcgttgctc gcttatcgcg atggcgcgcg tattcatgcc gggacgcggt
     5581 cagccgcgcc gcagatggaa aaagccgacg cgcagcttcg cttcctttgc gatgctggct
     5641 tttcggcagg tgacgcgacc tatgcgttga tggcaatcag ctacttcacc gtcggcgctg
     5701 ttcttgagca gcaagctagc gaggcagacg ccgaggagcg gggcgaagat cagttgacca
     5761 cctcagcgtc tacgatgccg gcgcgcctac agagcgcgat gaaaatcgtc tacgaaggcg
     5821 gtccggacgc ggcattcgag cgaggcctgg ctctcatcat cggcggtctt gaacaggtgc
     5881 ggctcagtcc ggcgtccagt cctgcgggtc gaacaaatct ggttcttgca ttggcggctg
     5941 gctcatgagg tcgtggtatt cgctggggcg catgtcgtgc tcaccctcaa ggaacggcag
     6001 ttccttggga cgacttacca gcacaagccc gctgctggtc tggtcgatgg cccatcccat
     6061 accgccttgc agtccgcgtg tgcggtctga gatttcgtct aaagcatcgc gtagttgacc
     6121 ccgcctgact gatcgaacct tcgttgatgc ttcttcccac gccgccttgc cccatacgtg
     6181 gggcgctagc gtgtcgatgt gcgccccgtt gccattgcca agcgaacggc caagccggat
     6241 gtttgagcac agccaacagt gcagcagctt ggcggcttca ctctccagct cgttgcgctc
     6301 gaaaagcgaa accttgacgt gttgctctcg aaaaaccgcc ccggtcaggc gcggattgac
     6361 ggcaacaaag atttccccag tcttgtgatt gaaccgactc gaaaggagat tgcagcggta
     6421 atcccagccg gtagcgtgat tgatttcccg tatctgcgcg ttacccattc ggttcaggct
     6481 ttccttgatt ctggcaagtg ccgccccgct ttctgggtcg tcatatccag catcaatcag
     6541 caggctgcgc aaactggttt tcttggtttt cagcggcctt ccatcatcgt tgtcaaagtc
     6601 caaagcaagg cgcaattgct ggcctatttc gccgggtggt tttgcctcga tcatcaagcc
     6661 gtcaatgcca agctgggcgc ttactgcaag caagattgat tggtcatcag ctccaagctg
     6721 ctcgaaccct tgaaagctga tttcactatc gccgaagcgg cgcggcggca catacagtcg
     6781 cccgcgcttc ttccccttcg tggggacgaa aagcccgtca aacacatgag cgggatcgaa
     6841 tttggcatag gccggtttaa tacgctgctg agtcatagcc cttgcccccc ttgttgccgc
     6901 tctcacgctt gatctcctgt agctctaccg gcagcaacac cccgccggtg tgtcgctgat
     6961 accagcggtc tagcggggtc gcgtcgtagt tctgcttact cacgccaaag cggacaaaaa
     7021 ggccgcgccg ctcgttgccg atgggcgcac gatcaaagcc ccggtcgctc aggcgctcgg
     7081 cttcctgctc cgtcatcttg gcgacatagc cgcaccatct ggcgttgtcg atcagggcag
     7141 acgcgccccg cgctgcctgc tgctggtcgg tctggccgtc gcgggcgctg cccttgttga
     7201 cgtggtgcag gtacaggaca gccgccccgg tggttgccgc gatgtgttcc aacacggaaa
     7261 caaggtgggc catgtcgccg ttgctgttct cgtcgaggct gtgaatgcgg ctcaatgtgt
     7321 ccagcacaat cagccgcgcc cctgcgctgt agtcgatgac gcggcgcaag tgcgcctcgt
     7381 ccatcacgtt caaccgcttg cccatgatcg gctcaagcat caggttctca gcgatggctt
     7441 cgcgggccga ctggttgagg tgctggccga tggcgtggac acgccgcaca agggcgggct
     7501 gtggatcttc gccagcgaga tagaccacgc gcccggtgtg cgccggggcc agtcctacaa
     7561 ggtcgccgcc tgccacactg caagcgattg acatggccgc ttcaagagca aaaaagctct
     7621 tgccagttgc gccaggtgca acaagtgcgc ccacggttcc ggccaagaat ccgggccaga
     7681 tgaaatcaag ttctggcggc tcattggtga aggccgccat aatgtctaaa gccatgctgt
     7741 tctcctgtgt agaacggtta tggttaggcg ctgccgggtg ttcccgcacc ctgccgcgcc
     7801 gcttctgcct tcgccttggc ggcatagccc gcttccagac tggcggcatc ggtcggcgtg
     7861 tcgccgtcga tgggcaggcc agaagcgaac atgagcgcat cacgtagggc aatctctgct
     7921 tgcccgtact ggcgtccctg aaaggcgctg tggccttccg tgccggtgtc ccgcacggtg
     7981 cgcccgccgt cgatgatgcg ccactcgtac caagcgtttt ccggctcgcc gtagacctcg
     8041 atcatcgcgc ccaaggaatc cggggcggga atggtgtaga tcgttttcat ttcgcaatcc
     8101 ccaagaacac ggccaagcaa cgctcaacct caagcagtgc gtccgcgtcg atgcgcccga
     8161 aagctggccc taccttgctg cgcagcaccg tcatcgcctt gtcgaccatc acctgcgatg
     8221 gcttctgcaa accgttctct gcgctcggct gaaccgtgac gcgcagcaac ggcgcggcat
     8281 taagtgtgct ggtgacaggc agcacggtaa cgctgccgtg ctcgctgaac tggttggcct
     8341 gaaccacaag cgcaggccgt ggcttgccaa agtcgccctg catggcgatg gtcacaaggt
     8401 cgccgcgcat cattccgtcc agccgtccac gtctgccaag gcttcatcca tgaactgctg
     8461 catggcggtg tcggcgctgt cggcctgagc cacaagcagg cattggcggc ggcactcctc
     8521 cgcgaagtcg gghcggcgcg tatccggcac ccagatttga accggacgaa gccccgccat
     8581 gcgtaatgca tcgcgatgct tctgaacccg ctgattaacg tgtgctgttg ccatttccaa
     8641 gcctccaatt aagttacatg caacattgta cgcacctcgc aagttacatg caacaggtat
     8701 ttaaagactc atccccggcg agttatcgcg ctcgatcttc tgcgccttgg cctgctcctg
     8761 ctggcgtaca ggctcgcgcc cttccagctt ctcccactgc tggcggtact tcccaagctc
     8821 ggcggcttgg cggtcgcctg cggcgctgta ggcatagcgg gcggtgcgct gcgcgtagtc
     8881 gttccagtcg cggccctcgt ccttctgccg ggtggccggg gcgcactggc ggatagcgcc
     8941 ctcgatggcg gcttgatcgt ggccggtgac gcgcatacgc acggcaatca tggaatccaa
     9001 gcgggacaag tccacctcgc cgccacgctg ccgcttgagc acgtcgcgat aatgccgctg
     9061 gtaggcgtcg atcgcgctgc cgctggccgc tgcaagctcc aaggcgggct tggctttgct
     9121 gcgctcgggc tgctgcgcct tcaaggccgc ttgccgctga tactcggcgt cgatctggct
     9181 ggacaaggcc agcgccttga tgcactcccg gcgctcggcc ttgagcaagc gcacctcggg
     9241 atagctgccg tcctcccgct ggtgcttggg cttgcggttc tcgtagccgg gtgcacggtg
     9301 cgggtggatg gctcccgata gcttcgggtc gccgtattca cggttcaggg catcgctcag
     9361 ccggttgccc acgtccttat cgtgggccgt ccccagcttc ggcacggtga tgatggcctg
     9421 atagttgccg gggctggatt caagcacggc ggcgggctgg tagccgtcgc ggataagccg
     9481 ctccagcttc tcccggttca tgtcgtcgat gaggatgtga tgcttcttgt ccgatagcgg
     9541 cgtgtagtag aggttttcgc cccggcgctg taggcgctgc atctccggcg tgcgctgctc
     9601 gatctcctgc ggtgtgaagc cccgcgtgat gccgtccttc ttgtcgagga tgaaggtttg
     9661 cttgctgccg tcctcgcgca tcttgataga cgttacccgg taacgctctg cgcctaccgc
     9721 ttccgcgtac cgctcgaact gctttagctg ctccgatttc atcgcctgca ccctttcctg
     9781 ctgtatccgc tgccgctcct gctggatgct ctcctgaagc tctggatttg tgatcttgaa
     9841 gccgtgctct gctgctaact tcccgcacat ggccttgtat tcgtcgttcc ccatcacggt
     9901 gaagctgccc cacttctgcg ccgatagctg taacgcggca agcgtgctgt cccggttgcg
     9961 ccagtcgtga atatcgatgc ttttgccctt gtccacgaaa gacacacccc cgccccgccc
    10021 cgcttcttcc ttccggctgt aatgcacctg ctggcccacg atttcaggct gataggcgcg
    10081 aatgtctcgc ggcgtcggcg gctcgctgcg gtcgccctcg atgcgctgcg gctcgctggc
    10141 ccggtgtcgc cactgctcgg caagctccgg gcttttctgc atccgctgcc actgctccag
    10201 gtcgggatag ggtcggaacc gctgccggtg ctgctcgcgc tccttctggt gcttctcctt
    10261 caaggccgct ttctctgcgg cctgctcggc ggcaatcacg ctgcgcatgg cgttgagcac
    10321 ttcgcccttg cctttccagt ttccgcgcat gagttcatcg cggcgggcct tctgctgctc
    10381 ggcaagttgc ttgcgctcct gctcctgccg cttgtcctgt gccagcttgt ccgcgttctt
    10441 ctcggcgtag tgtgccttgc gcccggtgat gtagtccttc catcccggca cgtctggctt
    10501 gatcggctcc ggctcgcgcg gcgctacctg ctgacgctgc ggcgctggct ggtattcacc
    10561 tagccgcttc tgcatcttgg caaggctggc gttgcggtct acatcgctgg ccttcacgcc
    10621 aacatccccg acaaacacgg tcgccccgct gccggtcttt tcgtagcgca tccccttggc
    10681 tgctaactcg cggtgcaact gctcccacgt ctgcgcctgc ttgatgatgg ctgcgccgtc
    10741 ctcgatggcg atgcggtggg ctgacttctc cccggtgcgg ttctccatgt cccgcttctt
    10801 ctggtcgggc tggcgtggct tttccgggtc gtatggcgct cggcctagct ccccgttctc
    10861 caacacctga tagcgcccgt tttgctcacg ctgccagcct tgggcgtgct caatccgtgc
    10921 aatggctttg tgtgccagtt caatatcaaa tccacggttc ttctcaacaa tcttgagcgt
    10981 ttcgggatgc acccgattga tgacgatgtg caggtggata ttgtccgtgt cggaatgaag
    11041 gccatagatg gcttggtggt ctttccaccc aagctcatcc atgaagatgc taaccgcttc
    11101 ctctacttgc tccgggcttg gctgttcgcc ttcgcgccaa ctcaagacgt agtgattgat
    11161 ggtgtccttg ctgcgcacgg cttcttgtga gagtgcaatc atctctgccg tctggctctt
    11221 ggggtcgtcc atcatgaaac cccgcgcccc ggcatacaga cacttttcct gcggactctc
    11281 cgactccggc gaacggacat agctggtaag ctggccgatg cgctgcgcct tgctcgcgga
    11341 cttcttcgga ttcggaacct tcttaacgat catcgtttag cttcctgaag tagtccttga
    11401 gcgcaacaag cgccccggct gtttccttgc tgtaaacgcc tccgctctcg ttgtggatgt
    11461 gcttcagcaa tccaccgatg cgccgcagct ccttgagcat cacggcgtcg gcgttggcaa
    11521 tgatcggcct gccgaagtag cggcggcgga caagctcgga catgctcaac ccggcaaggt
    11581 cggcgtcctc tttcaatctg gccttctccg ctgcggtcag ccgcacattg ataacggcgt
    11641 ccagcggttc cggcccctgc gtttcaaatg gcatcatgtc tccttctcgc cgggggcggg
    11701 ggcgggggcg gggtgtgtct tggacagtaa gaagcgaaag cgccgcactg tccggggtca
    11761 aggggcttgc cccttgcagg atgagccaga ccgtgagaag cgaagcgccg aatgggcagg
    11821 cgg
//
