LOCUS       NC_003114               3172 bp    DNA     circular CON 03-JAN-2024
DEFINITION  Citrobacter rodentium plasmid pCRP3, complete sequence.
ACCESSION   NC_003114
VERSION     NC_003114.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224723
KEYWORDS    RefSeq.
SOURCE      Citrobacter rodentium
  ORGANISM  Citrobacter rodentium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Deng,W., Li,Y., Vallance,B.A. and Finlay,B.B.
  TITLE     Locus of enterocyte effacement from Citrobacter rodentium: sequence
            analysis and evidence for horizontal transfer among attaching and
            effacing pathogens
  JOURNAL   Infect Immun 69 (10), 6323-6335 (2001)
   PUBMED   11553577
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Deng,W. and Finlay,B.B.
  TITLE     The 3 kb plasmid pCRP3 in Citrobacter rodentium, the causative agent
            of transmissible murine colonic hyperplasia, is almost identical to
            plasmid p9705 in Escherichia coli O157:H7
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3172)
  AUTHORS   Deng,W. and Finlay,B.B.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2000) Biotechnology Laboratory, University of
            British Columbia, Room 237, Wesbrook Building, 6174 University
            Boulevard, Vancouver, BC V6T 1Z3, Canada
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/03/2024 05:58:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 5
            CDSs (total)                 :: 5
            Genes (coding)               :: 5
            CDSs (with protein)          :: 5
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AF311902.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Citrobacter rodentium"
                     /mol_type="genomic DNA"
                     /strain="DBS100"
                     /db_xref="taxon:67825"
                     /plasmid="pCRP3"
                     /note="similar to Escherichia coli O157:H7 strain 9705 3kb
                     plasmid p9705"
     gene            527..682
                     /locus_tag="HKK02_RS00005"
                     /old_locus_tag="pCRP3p01"
     CDS             527..682
                     /locus_tag="HKK02_RS00005"
                     /old_locus_tag="pCRP3p01"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016570191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001490772.1"
                     /translation="MSVDFCDARQGGGAYGKTPAALASSGALLFAHMFFPALSPDSVDN
                     RITAFE"
                     /name="hypothetical protein"
     gene            complement(810..1004)
                     /locus_tag="HKK02_RS00010"
                     /old_locus_tag="pCRP3p02"
     CDS             complement(810..1004)
                     /locus_tag="HKK02_RS00010"
                     /old_locus_tag="pCRP3p02"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041621.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1288..1611
                     /gene="mobA"
                     /locus_tag="HKK02_RS00015"
                     /old_locus_tag="pCRP3p04"
     CDS             1288..1611
                     /gene="mobA"
                     /locus_tag="HKK02_RS00015"
                     /old_locus_tag="pCRP3p04"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(2000..2428)
                     /locus_tag="HKK02_RS00020"
                     /old_locus_tag="pCRP3p05"
     CDS             complement(2000..2428)
                     /locus_tag="HKK02_RS00020"
                     /old_locus_tag="pCRP3p05"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            2720..3148
                     /locus_tag="HKK02_RS00025"
                     /old_locus_tag="pCRP3p06"
     CDS             2720..3148
                     /locus_tag="HKK02_RS00025"
                     /old_locus_tag="pCRP3p06"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     Typing          1040..1279
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(252..781)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.03"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          1289..1608
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.23e-62"
ORIGIN
        1 ttgagattca ttcagtctcg tcgtaatctc actattgtaa aaacgaaaaa accaccctgg
       61 caggtggttt ttcgaaggtt agttaatcct ggcagattct ctaaccgtgg taacagtctt
      121 gtgcgagaca tgtcaccaaa tactgtcctt tcagtgtagc ctcagttagg ccgccacttc
      181 aagaactctc gttacatctc tcgcacatcc tgcttaccag tggccgttgc cagtggcgtt
      241 aagtcgtgtc ttaccgggtt ggattcaaga cgatagttac cggataaggc gcagcggtcg
      301 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgggccg
      361 agataccaac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
      421 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
      481 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctggcttga gcgtcgattt
      541 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg cctgcggcgc tggcttcttc
      601 cggtgctttg ctttttgctc acatgttctt tccggcttta tcccctgatt ctgtggataa
      661 ccgtattact gcttttgagt gagctgacac cgctcgccgc agtcgaacgt ccgagcgtag
      721 cgagtcagtg agcgaggaag cggaagagcg ccttatgtga cattttctcc ttacgctctg
      781 ttgtgccgtt cggcatcctg tcctgagcgt tatctctctg tgctattttc tacttcaaag
      841 cgtgtctgga tgctgttctg gagttcttct gcgagttcgt gcagcttctc acacatggta
      901 gcctgttcgt cagcatcgag tgcgtccagc ttttcgagca gcgtcaggct ttggcttttt
      961 atgaatcctg ccatgttgag tacggcttgc tgctgcttgt tcatcttttc gttttctccg
     1021 ttctgtctgt catctgcgtt gtgtgattat atcgcgtacc acttttcgac tgttttaccg
     1081 ccggtattct gccgtctgac gctttgacgg gtatttctgc ctgacaatac tgtcactgcc
     1141 aaaaaactgc cgtgcctttg tcggtaattc gaacttgctg acaggacagg atgtgtaatt
     1201 gttataccgc gcatacatgc acgctattac aattaccctg gtcagggctt tgccccgaca
     1261 cccatgtcag atacggagcc atgttttatg acaaaacgaa gtggaagtaa tacgcgcagg
     1321 cgggctatca gtcgccctgt tcgtctgacg gcagaagaag accaggaaat cagaaaaagg
     1381 gctgctgaat gcggcaagac cgtttccggt tttttacggg cggcagctct cggtaagaaa
     1441 gttaactcac tgactgatga tcgggtactg aaagaagtta tgagactggg ggcgttacag
     1501 aaaaaactct ttatcgacgg caagcgtgtc ggggacaggg agtatgcgga ggtgctgatc
     1561 gctattacgg agtatcaccg tgccctgtta tccaggctta tggcagatta gcttcccgga
     1621 gagaaaactg tcgaaaactg acggtatgaa caccgtaagc tcccaaagtg atcaccattc
     1681 gctttcatgc atagctatgc agcgagctga aaacgatcct gacgcatcct tcctgttttt
     1741 ccggggtaaa acatctcttt ttgcggtgtc tgcgtcagaa tcgcgttcag cgcgtttcag
     1801 tggtgcgtac aattaaggga ttatggtaaa tatatgagct atgcgataac tttaactgtg
     1861 aagcgatgaa cccattacag gcaaagccaa ttactcctga cagtggttta gccagaagca
     1921 gggctaccaa gaccaatgca ataagtaata tatcgttttg ctatcgtgca tcgctcgcgc
     1981 tcagttccat tgtgcttttt taagctgtcg tttttcttac ggtatatacc ggttttttat
     2041 ggcgtggttt cttaacttgt tcagctactg atggacccat tgtatctagg tagtcaacta
     2101 gctttgttag atcataaata ttgcgaccac catatcggcg atcactcttc gatgttggtt
     2161 tcttgtccaa gagattagct ttttcaagat cattgatagc tctctgaaca gtccgtacag
     2221 aaacccccat acgtatggct agactttcca ttgacggatg cggccactct tgcaaactcc
     2281 accagtgaac gatcaggtta agtagtgtgt taaaggccac tgaagttagc tttttctcgt
     2341 tttgtataaa aaacaatacg gtaggcactg ccgtccagcc aagagacaaa ccgccagctt
     2401 tccatttatt cttaacggag taagtcattg attttcctaa gctccaaaat atttaaagta
     2461 tatattatat ggtatattca tatgaatagg gtgacactgg cgccattatt gtgcaaccaa
     2521 aaaagactac tctgaaaacg aggaaaagat tttttcctgc ctgaattaga tacggagtta
     2581 gcgatatgaa aaccgaacaa cgtcatgatc ttgttaaaga tattgaggtt tttggcgtat
     2641 ccttgtctct gttgatttcc agagcgaatg agaagtctgt tacaatgcca tctggtctaa
     2701 gtcgggagca gagaagagca tgggcagcgg agcaggcgcg caaaatccac aattgaatat
     2761 tgtctcattc tctgagacct tcaaccttta ttacacatcc agatattctg caaaaacact
     2821 cgataaaatc gatgatttca ttgagcattt tgaaaaatac aatctctttg gcgatccttt
     2881 aaaaggatat ccagcttgga ctggcaaagt atcgccatcg tggaaagtgc ctgatcatta
     2941 cgaaaacaaa gaagctattg agaagtatgc tagagctaac aaattatggc atgctcattt
     3001 aggcgatccg gtttttaaag atacgtttca tgggaaatac aaggtttctg actgggttat
     3061 tcatttccag cggctgacac cgaaccatat aaagctttta gagcttggat accatgaccc
     3121 aatgaagcta cctcaaaact ttgaatgatc gagcggcagg ctaaatgaaa tc
//
