LOCUS       NC_002773               3179 bp    DNA     circular CON 09-SEP-2023
DEFINITION  Shigella flexneri 2a plasmid p2457TS2, complete sequence.
ACCESSION   NC_002773
VERSION     NC_002773.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14224792
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri 2a
  ORGANISM  Shigella flexneri 2a
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Wang,H., Feng,E., Liao,X., Su,G. and Huang,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2001) Institute of Biotechnology, 20 Dongdajie
            Street, Fengtai District, Beijing 100071, P.R. China
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 09/09/2023 03:45:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AY028316.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri 2a"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:42897"
                     /plasmid="p2457TS2"
                     /note="2Md plasmid"
     gene            complement(99..1208)
                     /gene="wzz(fepE)"
                     /locus_tag="HPE64_RS00005"
                     /old_locus_tag="p2457TS2_00005"
     CDS             complement(99..1208)
                     /gene="wzz(fepE)"
                     /locus_tag="HPE64_RS00005"
                     /old_locus_tag="p2457TS2_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="WP_005065596.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVIL
                     FFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFALF
                     IKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKNDKS
                     SLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEILALD
                     EIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIATKLNI
                     KKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPSNAIIVI
                     LSSVIGGMLACGYILLRHAMEARKMSVIE"
                     /name="wzz(fepE)"
     gene            2089..2253
                     /locus_tag="HPE64_RS00010"
                     /old_locus_tag="p2457TS2_00010"
     CDS             2089..2253
                     /locus_tag="HPE64_RS00010"
                     /old_locus_tag="p2457TS2_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000090200.1"
                     /translation="MARCIKSQSLTLLEKLDALDADEQAAMCKRLHELAEELQNSIQIR
                     FEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2899..3033
                     /locus_tag="HPE64_RS00015"
     CDS             2899..3033
                     /locus_tag="HPE64_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071606675.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY"
                     /name="replication initiation protein"
     Typing          complement(1540..1797)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.385"
                     /coverage="89.0"
                     /evalue="3.4e-112"
     Typing          complement(1922..2020)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(2334..2420)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          2445..2971
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.956"
                     /coverage="98.0"
                     /evalue="2.2599999999999997e-176"
ORIGIN
        1 tctagagtag aagactacgg ctgtaaggga atgatctcaa gaagatcctt tgaacttttt
       61 ataaaagttg cttataaaat gatgcatcag cacaactttt actcaatcac actcatcttt
      121 ctggcctcca tggcatgacg taaaagtata tagccacatg caagcatccc tccaatgaca
      181 gaagaaagaa ttacaattat ggcattactt ggcccatcct ttctgaccgg taacgttggg
      241 ctcaattgat actgaaatgg cataaaatca acatccccca ctttcgccac aaccaattga
      301 ttcacaacat attgacgatt tcgcaactcc ccactcaatt ccgaaacatc cttgattgat
      361 tttttgatgt tcaatttagt tgctatacca tcagaaccga gagccacagg aaaatctggg
      421 tcatccttca taatctgacc attgctgtat acaggttttt ttattccagc cgcattagca
      481 acctccagtg aataattgag gcgtcgaata tctgcattca gctggtttct aatttttatc
      541 tcatccagtg caagaatttc tttttcaaca ttagttttaa cttctagttt atttcttata
      601 tcttccatca aatcccttac aacaagggaa gaaacataat taatataccc agacaacaca
      661 tcgtgagctt ctttacttgt tggagcagta aaactcagag tccatgatac atacaatgat
      721 gacttgtcgt ttttcttact ggcattatca tcaatcgact tcatattttt actcaactca
      781 acaattgcac gatgcagttc gagagcgcta atgttattct ttttaataat ctccataaca
      841 taaggagatg atctcatata ctcattcaac gaattaacgg actgaaattt cttaataaac
      901 agagcaaatg cgctatcacg attgatatca aaatccactc ccagaacatg aagcttggaa
      961 agctccttat ttaattcact ccattgaaca gactcagcag gagtaaccac agcagaacta
     1021 gtccattttt gtggcaaaac aaaagaaaca agaaagccaa tacaagcaaa aaatagaatc
     1081 accccaacaa tttttatttt tgctgcccat aaaacgctaa tcaagccaaa aaggtcaatt
     1141 tcttcattac cattcggcaa catgttgtaa ttagaaaaat gttcgtcaga gacctttgag
     1201 attttcattg aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta
     1261 ggaaaaaacc taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc
     1321 taaaacgcgc tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg
     1381 taaaccggaa aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg
     1441 aatgacgatc ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt
     1501 tcgtgaatcc ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg
     1561 ggcgtcctgt aaaaaaacac accgcgccgc tagcagcacc cctaatataa gataatgttt
     1621 tttttatgaa aatagtcagt accaccccta aaaagcggtg ttggcgcagc tacaacaccg
     1681 cgccgacacc gcttttttaa atatcacaaa gagagcaaga agaactaatt tgtcataact
     1741 ctctatttat aaagaaaaat cagcaaaaac ttgtttttgc gtggggtgtg ggcatttcag
     1801 gcatgagaac agactcgcgt ttttccggag tatcagcgga attattgatt aagaaacact
     1861 cgacgcagag cgaaatttta tgcaacagcg gctgcactgc acagggatta tttatcagtt
     1921 gatatgctgt gccatgatac gtcctaaaga aacaggacag gatgtgcaat tgtaataccg
     1981 tcgcccgcaa cgtcttacaa ttaccacctg ctcagggtcc cccccccgac acccggaact
     2041 gacggagaac aacggaaggt gaacaaataa caacaaactg cgctgaatat ggcgcgatgt
     2101 atcaaaagcc agagcctgac actgcttgaa aaactggatg ctctggatgc cgacgaacag
     2161 gcggccatgt gcaaacgact gcacgaactc gcagaagaac tccagaacag catccagatt
     2221 cgctttgagg ccgaaagtga aacaggaaca taacgaagct gacggaggcg gtcacggctt
     2281 tcccgtgggc gttttcgtca gcagacaagc ccgtcagggc gcgtcagggg gttttagcgg
     2341 gtgtcggggc gcagccatga cccagtcacg tagcgataac ggagtgtata ctggcttaat
     2401 tatgaggcgg cagagcagat ggtgagagaa cagcgacata tgaggtgtga aatgctgcac
     2461 agatacgtaa ggagaaaatg cagcttccgg cgctcttccg cttcctcgct cactgactcg
     2521 ctgcgctcgg tcgttcggct gcggcgagcg gtgtctgctc acgcaaagac ggatatacgg
     2581 ttatccacag aatcagggga taacaccaga aaaaacatgt gagcaaaaaa gaggccccga
     2641 aaaaaggccg cgccggtggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2701 aatcgacgct caagtcagag gtggcgaaac ccgacaggac ttaaagatac caggcgtttc
     2761 cccctggtgg ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc
     2821 cgctgttatg gccgcgttcg tctcattcca cgcctgacac tcagttccgg gtaggcagtt
     2881 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2941 ggtaactatc gtcttgagtc caacccggta agacacgact taacgccatt ggcagcggcc
     3001 actggtaaac aggatgtgcg agagatgtat tgagagttct tgaagtggca gccttactga
     3061 ggctacacta gaaggacaga tttggtgaca tgtctcgcac aagactgtta ccacggttca
     3121 gaagttcccc aacttactga accttcgaaa aaccacctcc ccaggtggtt ttttcgttt
//
