LOCUS       NC_001767               4439 bp    DNA     circular CON 11-SEP-2023
DEFINITION  Staphylococcus aureus plasmid pKH6, complete sequence.
ACCESSION   NC_001767
VERSION     NC_001767.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14228816
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Moon,K.H., Lee,D.W., Yoon,S.J., Kim,W.K., Shin,C.K. and Im,S.H.
  TITLE     Complete nucleotide sequence of pKH6, a tetracycline-resistance
            plasmid from multidrug-resistant Staphylococcus aureus SA2
  JOURNAL   Kor. J. Appl. Microbiol. Biotechnol. 24, 423-426 (1996)
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Moon,K.H., Lee,D.W., Yoon,S.J., Kim,W.K., Shin,C.K. and Im,S.H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-1995) Kyung-Ho Moon, Pharmacy, Kyungsung
            University, 110-1, Daeyon-dong, Nam-gu, Pusan, 608-736, Korea
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 09/11/2023 03:42:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            U38428.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1280"
                     /plasmid="pKH6"
     gene            join(3654..4439,1..159)
                     /locus_tag="HTY17_RS00005"
                     /old_locus_tag="pKH6_p1"
     CDS             join(3654..4439,1..159)
                     /locus_tag="HTY17_RS00005"
                     /old_locus_tag="pKH6_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_000277654.1"
                     /translation="MYKNNHANHSNHLENHDLDNFSKTGYSNSRLDAHTVCISDPKLSF
                     DAMTIVGNLNRDNAQALSKFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADSWD
                     RRNMRIEFNPNKLTRDEMIWLKQNIISYMEDDGFTRLDLAFDFEDDLSDYYAMSDKAVK
                     KTIFYGRNGKPETKYFGVRDSNRFIRIYNKKQERKDNADAEVMSEHLWRVEIELKRDMV
                     DYWNDCFSDLHILQPDWKTIQRTADRAIVFMLLSDEEEWGKLHRNSRTKYKNLIKEISP
                     VDLTDLMKSTLKANEKQLQKQIDFWQHEFKFWK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            321..1700
                     /gene="tet(K)"
                     /locus_tag="HTY17_RS00010"
                     /old_locus_tag="pKH6_p2"
     ARG             322..1697
                     /name="tet(K)"
                     /gene="tet(K)"
                     /gene_name="tetracycline efflux MFS transporter Tet(K)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1886..3127
                     /gene="mobV"
                     /locus_tag="HTY17_RS00015"
                     /old_locus_tag="pKH6_p3"
     CDS             1886..3127
                     /gene="mobV"
                     /locus_tag="HTY17_RS00015"
                     /old_locus_tag="pKH6_p3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010889940.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_010890196.1"
                     /translation="MSYSIVRVSKVKSGTNTTGIQKHVQRENNNYENEDIDHSKTYLNY
                     DLVNANKQNFNNLIDEKIEQNYTGKRKIRTDAIKHIDGLITSDNDFFDNQTPEDTKQFF
                     EYAKEFLEQEYGKDNLLYATVHMDEKTPHMHYGVVPITDDGRLSAKEVVGNKKALTAFQ
                     DRFNEHVKQRGYDLERGQSRQVTNAKHEQISQYKQKTEYHKQEYERESQKTDHIKQKND
                     KLMQEYQKSLNTLKKPINVPYEQETEKVGGLFSKEIQEAGNVVISQKDFNEFQKQIKAA
                     QDISEDYEYIKSGRALDDKDKEIREKDDLLNKAVERIENADDNFNQLYENAKPLKENIE
                     IALKLLKILLKELERVLGRNTFAERVNKLTEDEPKLNGLAGNLDKKMNPELYSEQEQQQ
                     EQQKNQKRDRGMHL"
                     /name="mobV"
     Typing          3655..4439
                     /name="rep_cluster_1118"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1887..3124
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.758"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcttcaca gaaattctag aacaaaatat aagaatttga taaaagaaat ttcgccagtc
       61 gatttaacgg acttaatgaa atcgacttta aaagcgaacg aaaaacaatt gcaaaaacaa
      121 atcgattttt ggcaacatga atttaaattt tggaaatagt gtacatatta atattactga
      181 acaaaaatga tatatttaaa ctattctaat ttaggaggat ttttttatga agtgtctatt
      241 taaaaatttg gggaatttat atgaggtgaa agaataattt acccctataa actttagtca
      301 cctcaagtaa agaggtaaaa ttgtttagtt tatataaaaa atttaaaggt ttgttttata
      361 gcgttttatt ttggctttgt attctttcat tttttagtgt attaaatgaa atggttttaa
      421 atgtttcttt acctgatatt gcaaatcatt ttaatactac tcctggaatt acaaactggg
      481 taaacactgc atatatgtta actttttcga taggaacagc agtatatgga aaattatctg
      541 attatataaa tataaaaaaa ttgttaatta ttggtattag tttgagctgt cttggttcat
      601 tgattgcttt tattggtcac aatcactttt ttattttgat ttttggtagg ttagtacaag
      661 gagtaggatc tgctgcattc ccttcactga ttatggtggt tgtagctaga aatattacaa
      721 gaaaaaaaca aggcaaagcc tttggtttta taggatcaat tgtagcttta ggtgaagggt
      781 taggtccttc aataggggga ataatagcac attatattca ttggtcttac ctacttatac
      841 ttcctatgat tacaatagta actatacctt ttcttattaa agtaatggta cctggtaaat
      901 caacaaaaaa tacattagat atcgtaggta ttgttttaat gtctataagt attatatgtt
      961 ttatgttatt tacgacaaat tataattgga cttttttaat actcttcaca atcttttttg
     1021 tgatttttat taaacatatt tcaagagttt ctaacccttt tattaatcct aaactaggga
     1081 aaaacattcc gtttatgctt ggtttgtttt ctggtgggct aatattttct atagtagctg
     1141 gttttatatc aatggtgcct tatatgatga aaactattta tcatgtaaat gtagcgacaa
     1201 taggtaatag tgttattttt cctggaacca tgagtgttat tgtttttggt tattttggtg
     1261 gttttttagt ggatagaaaa ggatcattat ttgtttttat tttaggatca ttgtctatct
     1321 ctataagttt tttaactatt gcattttttg ttgagtttag tatgtggttg actactttta
     1381 tgtttatatt tgttatgggc ggattatctt ttactaaaac agttatatca aaaatagtat
     1441 caagtagtct ttctgaagaa gaagttgctt ctggaatgag tttgctaaat ttcacaagtt
     1501 ttttatcaga gggaacaggt atagcaattg taggaggttt attgtcacta caattgatta
     1561 atcgtaaact agttctggaa tttataaatt attcttctgg agtgtatagt aatattcttg
     1621 tagccatggc tatccttatt attttatgtt gtcttttgac gattattgta tttaaacgtt
     1681 ctgaaaagca gtttgaatag ttatattata ttttggttta gaactatgag tggctagcat
     1741 tttgccactc attttttgcg ttagcaaaaa caggtttaag cctcgcagag cacacgtatt
     1801 aacgacttat taaaaataag tctagtgtgt tagacttaaa ctattaaata cacatgaaac
     1861 ctttgtgctt aggagtgatt tttatatgtc ttattccatt gttagagttt caaaagttaa
     1921 atctggaaca aatacaacgg gcatacaaaa acatgttcaa agagaaaata ataattatga
     1981 aaatgaagat atagaccata gtaaaactta cttaaattat gatttggtaa atgctaataa
     2041 acagaatttt aataacttga ttgatgaaaa aatcgaacag aattatacag gcaaaagaaa
     2101 aattagaaca gacgcgatta aacacattga tggtttaatt acatcagaca atgatttctt
     2161 tgataatcaa acgccagaag atacaaagca gttttttgaa tatgctaaag agtttttaga
     2221 acaagaatac ggtaaagata atttattata tgcaacagtt cacatggacg aaaaaacacc
     2281 acatatgcat tatggcgttg ttccaataac tgatgatggt cgtttaagtg ctaaagaagt
     2341 tgtaggtaat aaaaaagctt taacagcgtt tcaagataga tttaatgagc atgttaaaca
     2401 acgaggatat gatttagaac gtgggcaatc aagacaagta acaaatgcta aacatgagca
     2461 aataagtcag tataaacaaa aaacagaata tcataagcaa gaatatgaac gtgagagcca
     2521 aaaaacagac catataaagc aaaagaacga taaattaatg caagagtacc aaaaatcgtt
     2581 aaatacgctt aaaaagccta taaatgttcc gtatgagcaa gaaactgaaa aagtaggtgg
     2641 tttatttagc aaagaaatac aagaagctgg aaatgttgta ataagccaaa aagatttcaa
     2701 tgaatttcag aaacagataa aagctgctca agatatttcg gaagattacg agtatataaa
     2761 gtctggtaga gccttagatg ataaagataa ggaaatacga gagaaagatg atttattaaa
     2821 taaagcagtt gagcgtattg aaaacgcaga cgataatttt aaccaacttt acgaaaatgc
     2881 aaagccactt aaagagaata tagaaatagc gttaaagctt ttaaaaatct tactaaaaga
     2941 gttagaacga gttttaggaa gaaatacctt tgcggaaaga gttaataagt taacagaaga
     3001 tgaaccaaaa ctaaatggtt tagcaggaaa cttagataaa aaaatgaatc cagaattata
     3061 ttcagaacag gaacagcaac aagaacaaca aaagaatcaa aaacgagata gaggtatgca
     3121 cttatagaac atgcatttat gccgagaaaa cttattggtt ggaatgggct atgtgttagc
     3181 taacttgtta gcgagttggt tggacttgaa ttgggattaa tcccaagaaa gtaccaaccc
     3241 aacaacacat aaagccctgt aggttccgac caataaggaa attggaataa agcaataaaa
     3301 ggagttgaag aaatgaaatt cagagaagcc tttgagaatt ttataacaag taagtatgta
     3361 cttggtgttt tagtagtttt aactgtttac cagataatac aaatgcttaa ataaaaaaag
     3421 acttgatctg attagaccaa gtcttttgat agtgttatat taataacaaa ataaaaagga
     3481 gtcgctcacg ccttgaccaa agtttgtgaa cgacatcatt caaagaaaaa aacactgagt
     3541 tgtttttata atcttgtata tttagatatt aaacgatatt taaatataca tcaagatata
     3601 tatttgggtg agcgattcct taaacgaaat tgagattaag gagtcgattt tttatgtata
     3661 aaaacaatca tgcaaatcat tcaaatcatt tggaaaatca cgatttagac aatttttcta
     3721 aaaccggcta ctctaatagc cggttggacg cacatactgt gtgcatatct gatccaaaat
     3781 taagttttga tgcaatgacg atcgttggaa atctcaaccg agacaacgct caagcccttt
     3841 ctaaatttat gagtgtagag ccccaaataa gactttggga tattcttcaa acaaagttta
     3901 aagctaaagc acttcaagaa aaagtttata ttgaatatga caaagtgaaa gcagatagtt
     3961 gggatagacg taatatgcgt attgaattta atccaaacaa acttacacga gatgaaatga
     4021 tttggttaaa acaaaatata ataagctaca tggaagatga cggttttaca agattagatt
     4081 tagcctttga ttttgaagat gatttgagtg actactatgc aatgtctgat aaagcagtta
     4141 agaaaactat tttttatggt cgtaatggta agccagaaac aaaatatttt ggcgtgagag
     4201 atagtaatag atttattaga atttataata aaaagcaaga acgtaaagat aatgcagatg
     4261 ctgaagttat gtctgaacat ttatggcgtg tagaaatcga acttaaaaga gatatggtgg
     4321 attactggaa tgattgcttt agtgatttac atatcttgca accagattgg aaaactatcc
     4381 aacgcactgc ggatagagca atagttttta tgttattgag tgatgaagaa gaatgggga
//
