LOCUS       NC_001395               2475 bp    DNA     circular CON 03-JAN-2024
DEFINITION  Staphylococcus aureus plasmid pT48, complete sequence.
ACCESSION   NC_001395
VERSION     NC_001395.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14227788
KEYWORDS    RefSeq; 23S ribosomal RNA methylase; circular; complete genome;
            lincosamide resistance; macrolide resistance; methylase enzyme;
            streptogramin resistance.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Catchpole,I., Thomas,C., Davies,A. and Dyke,K.G.
  TITLE     The nucleotide sequence of Staphylococcus aureus plasmid pT48
            conferring inducible macrolide-lincosamide-streptogramin B
            resistance and comparison with similar plasmids expressing
            constitutive resistance
  JOURNAL   J Gen Microbiol 134 (3), 697-709 (1988)
   PUBMED   3141573
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/03/2024 06:19:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 3
            CDSs (total)                 :: 3
            Genes (coding)               :: 3
            CDSs (with protein)          :: 3
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            M19652.1.
            Draft entry and computer-readable sequence kindly provided by
            I.Catchpole 06-JUL-1988.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="T48"
                     /db_xref="taxon:1280"
                     /plasmid="pT48"
     gene            complement(64..540)
                     /locus_tag="HTN12_RS00005"
                     /old_locus_tag="pT48_p1"
     CDS             complement(64..540)
                     /locus_tag="HTN12_RS00005"
                     /old_locus_tag="pT48_p1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664727.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA];
                     GO:0006276 - plasmid maintenance [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="WP_000664727.1"
                     /translation="MKERYGTVYKGSQRLIDEESGEVIEVDKLYRKQTSGNFVKAYIVQ
                     LISMLDMIGGKKLKIVNYILDNVHLSNNTMIATTREIAKATGTSLQTVITTLKILEEGN
                     IIKRKTGVLMLNPELLMRGDDQKQKYLLLEFGNFEQEANEKQENALSDYYSFKD"
                     /name="replication/maintenance protein RepL"
     gene            complement(988..1722)
                     /gene="erm(C)"
                     /locus_tag="HTN12_RS00010"
                     /old_locus_tag="pT48_p2"
     ARG             complement(992..1722)
                     /name="erm(C)"
                     /gene="erm(C)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1783..1842)
                     /gene="ermCL"
                     /locus_tag="HTN12_RS00015"
     CDS             complement(1783..1842)
                     /gene="ermCL"
                     /locus_tag="HTN12_RS00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_002497446.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
                     /name="ermCL"
     ARG             complement(989..1722)
                     /name="ErmC"
                     /gene="ErmC"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="99.18"
                     /coverage="100.0"
     Typing          complement(55..540)
                     /name="rep_cluster_1947"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.591"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcttttga tctacattaa gcgtgcaggt tttttgtcta tttgtagacg attttatgtt
       61 atactagtcc ttgaaagaat aataatcaga taatgcattt tcttgttttt catttgcctc
      121 ttgctcaaag ttcccaaatt cgagtaagag gtatttttgt ttttggtcgt cgcctctcat
      181 tagtagttca gggtttaaca ttaatactcc agtttttctt tttataatat ttccttcttc
      241 taagatttta agtgttgtta ttactgtttg tagacttgtt cctgtagctt ttgctatttc
      301 tcttgttgta gctatcattg tattgttact taagtggaca ttatctagga tatagttaac
      361 gattttaagt ttttttccgc caatcatatc taacatactt attaattgca ctatatatgc
      421 ctttacgaag ttaccagacg tttgtttacg gtataacttg tctacctcta tgacttctcc
      481 actttcttcg tctatgagcc tctgagagcc tttatagact gttccatatc tttctttcat
      541 ctttttctca ctccttattt taaactattc taactatatc ataactgttc taaaaaaaaa
      601 agaacatttg ttaaaagaaa ttagaacaaa atgagtgaaa aattagaaca aacaaattcc
      661 ttataaacct tatcatctca acctatatta agattttacc tagttgaatc ttcttttcta
      721 tataaagcgt cggagcatat cagggggtta tctaacgtaa atgctaccct tcggctcgct
      781 ttcgctcggc attgacgtca gatactgcac cccctgaacc cccatgctcc aacagcaaaa
      841 aggaaacttt ttgctgcttt tccgacgctt attcgcttcg ctcatattta tatagaaaag
      901 aagcgaatgc gcaaaagaca taatcgattc acaaaaaata ggtacacgaa aaacaagtta
      961 agggatgcag tttatgcatc ccttaactta cttattaaat aatttatagc tattgaaaag
     1021 agataagaat tgttcaaagc taatattgtt taaatcgtca attcctgcat gttttaagga
     1081 attgttaaat tgattttttg taaatatttt cttgtattct ttgttaaccc atttcataac
     1141 gaaataatta tacttctgtt tatctttgtg tgatattctt gatttttttc tatttaatct
     1201 gataagtgag ctattcactt taggtttagg atgaaaatat tctcttggaa ccatacttaa
     1261 tatagaaata tcaacttctg ccattaaaag taatgccaat gagcgttttg tatttaataa
     1321 tcttttagca aacccgtatt ccacgattaa ataaatctca tcagctatac tatcaaaaac
     1381 aattttgcgt attatatctg tacttatgtt ataaggtata ttaccaaata ttttatagga
     1441 ttggttttta ggaaatttaa actgcaatat atccttgttt aaaacttgga aattatcgtg
     1501 atcaacaagt ttattttctg tagttttgca taatttatgg tctatttcaa tggcagttac
     1561 gaaattacac ctctgtacta attcaagggt aaaatggcct tttcctgagc cgatttcaaa
     1621 gatattatca tgttcattta atcttatatt tgtcattatt ttatctatat tatgttttga
     1681 agtaataaag ttttgactgt gttttatatt tttctcgttc attataaccc tctttaattt
     1741 ggttataatg aattttgctt attaacgatt cattataacc acttattttt tgtttggttg
     1801 ataatgaact gtgctgatta caaaaatact aaaaatgccc atattttttc ctccttataa
     1861 aattagtata attatagcac gagctctgat aaatatgaac atgatgagtg atcgttaaat
     1921 ttatactgca atctgatgcg attattgaat aaaagatatg agagatttat ctagtttctt
     1981 tttttacaag aaaaaagaaa gttcttaaag gttttatagt tttggtcgta gagcacacgg
     2041 tttaacgact taattacgaa gtaaataagt ctagtgtgtt ttagacttta atgtttttta
     2101 aaggcattag tgcacttaag cgtcagagca tggctttatg ccgagaaaac tattggttgg
     2161 aatggcgtgt gtgttagcca aagctttggc gagttggttg ggggtttcat gggattaatc
     2221 ccatgaaagt accaactcaa caacacacta acgcctgttg gttccaacca ataggaaatt
     2281 ggaataagca attagtataa tgagagtata atgttggtat aacgttagta taatgatgct
     2341 ttttttcatt atatttttta tgtactttaa acctgcacgc ttatgcgaat tagaaaaagc
     2401 ttaatcgcat ttcatagatt gacctcccaa taactacgtg gtgttattgg gaggtcaatc
     2461 tatttcattt gcgtt
//
