LOCUS       MF554640                2826 bp    DNA     circular ENV 20-DEC-2017
DEFINITION  uncultured bacterium clone AA-103 plasmid pPICR, complete sequence.
ACCESSION   MF554640
VERSION     MF554640.1
KEYWORDS    ENV.
SOURCE      uncultured bacterium
  ORGANISM  uncultured bacterium
            Bacteria; environmental samples.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Ajayi,A.O. and Yost,C.K.
  TITLE     Characterization of antibiotic resistance plasmids extracted from
            three waste water treatment plants reveals the diversity of mobile
            elements carrying ciprofloxacin and meropenem resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Ajayi,A.O. and Yost,C.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2017) Department of Biology, University of Regina,
            3737 Wascana Parkway, Regina, Saskatchewan S4S 0A2, Canada
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SPAdes v. 3.10.1
            Sequencing Technology :: Illumina; Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="uncultured bacterium"
                     /mol_type="genomic DNA"
                     /isolation_source="Regina waste water treatment plant"
                     /db_xref="taxon:77133"
                     /clone="AA-103"
                     /environmental_sample=""
                     /plasmid="pPICR"
                     /geo_loc_name="Canada"
     CDS             194..397
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AUF80023.1"
                     /translation="MFNRSDRQVQKKALRGLFFCYELAIFSLRHGFMIREWLFVVLAKM
                     NWLDIHQIPVQNAALDGMSLMR"
                     /name="hypothetical protein"
     CDS             complement(501..1181)
                     /codon_start=1
                     /transl_table=11
                     /product="qnrB19"
                     /protein_id="AUF80024.1"
                     /translation="MASLLYKNTGIDMTLALVGEKIDRNRFTGEKVENSTFFNCDFSGA
                     DLSGTEFIGCQFYDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQ
                     GADFRGASFMNMITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATF
                     SGSDLSGGEFSTFDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGI
                     AVIG"
                     /name="qnrB19"
     CDS             complement(1239..1436)
                     /codon_start=1
                     /transl_table=11
                     /product="psp operon activator"
                     /protein_id="AUF80025.1"
                     /translation="MEIYPIRAHRIHIVITLDLREFQQQQEKDFLQTSLQQAKFNQKKA
                     AELLGLTYHQLRALLKKHQI"
                     /name="psp operon activator"
     CDS             2462..2698
                     /codon_start=1
                     /transl_table=11
                     /product="rep like helicase"
                     /protein_id="AUF80026.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="rep like helicase"
     ARG             complement(505..1145)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2124..2297
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1654..2145)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 tttggctttt ggcggtttag tggtgctgac gctgcgaaat cacctgccat tcacctgcgt
       61 gcatagccat gcagtcgcgc agaaatcatc aggggcgttt ttccgggata ctggtgagga
      121 aaggtgctgg aaacggccct gatgcagcag gattgcgctg agggcgcctg cgggggtgcg
      181 ggcaagggat gttatgttta atcggtctga ccgtcaggta caaaaaaaag cccttagggg
      241 gctttttttt tgctatgagt tagccatatt ttcgttaaga catggcttca tgattcggga
      301 gtggttattc gttgttctcg cgaaaatgaa ttggttagat attcatcaaa ttcctgtcca
      361 gaatgcagca ttagatggca tgtcgttgat gcggtaggtc aggatattcg tccactggag
      421 aaaacgtttc acaatgcttg ccaggagtcg tactggttaa aagtaaatcg tcaaacgcat
      481 ctcccggtgt aaacgctgaa ctaaccaatc acagcgatgc caagccgctc catgagcaac
      541 gatgcctggt agctgtctaa cttaacgcct tgtaaatcaa caccccgaat atctaagtca
      601 cctaactccg aattggtcag atcgcaatgt gtgaagtttg ctgctcgcca gtcgaaagtc
      661 gaaaactcgc cgccggagag atctgaacca ctgaacgtcg cacccagtac ctgagtcccc
      721 atccagcggt tttcccacag ctcacacttt tccaacacga ctttcgaaaa attggcgtag
      781 cttagattag tattagtgat atatgcgctg caaaaccagg tgcgcgtggt gatcatattc
      841 ataaagcttg caccgcggaa atctgcgcct tgtgcgcggc agtggcgaat ttcaatgccc
      901 aaggcactga cgttgcggaa atctgccatt gataaatcac agcttttgaa aatggcatct
      961 ttcagcattg cgcgactaaa attgcaccct ttctgacttt cgcgatcata gaactggcag
     1021 ccgataaatt cagtgccgct caggtcggca cctgaaaaat cgcagttaaa aaatgtacta
     1081 ttttcaactt tctcaccggt gaagcgattt ctgtcaattt tttcgccaac taatgccaga
     1141 gtcatatcta tgcctgtgtt tttatacagt aatgacgcca tggtaaacct tatgacgtta
     1201 tgcgtcaaac ccgccagtat gacacctgca aatgcgtatt agatctggtg tttcttcagc
     1261 aaggcacgta gctgatgata agtcagaccc agtaattcag cggctttttt ctggttaaat
     1321 ttagcctgct gtaagctggt ttgtagaaag tctttctctt gctgctgctg gaattcacgc
     1381 agatccagcg ttataacgat gtgtatccta tgtgctcgga ttggatagat ttccattacg
     1441 gtgaaggatc ttcttgagat cctttctgtg gcgtcgtaat ctcttgctct gtaaacgaaa
     1501 aaaccgccct gcaaggcggt ttttcgaagg ttctcagagc agcaactctt tgaaccgtgg
     1561 taactggctt acgaggagcg cagtaaccaa atctgttctt tcagtttagc cttaacaggc
     1621 gcataacttc aagactaact cctctaaatc agttaccagt ggctgctgcc agtggcgata
     1681 agtcgtgtct ttccgggttg gactcaagat gatagttacc ggataaggcg cagcagtcgg
     1741 actgaacggg gggttcgtgc atacagtcca tccaggagcg aactgccttc gcggaactga
     1801 gtgtcaggcg tggaatgaga taaacgcggc cataacagcg gaatgacacc ggtaaaccga
     1861 aaggcaggaa tgcgggggag cacgagggag ccaccagggg gaaacgcctg gtatctttaa
     1921 gccgcatcgg gtttcgccac cactgatttg agcgtcagat ttcatgatgt ttgtcagggg
     1981 ggcggagcct atggaaaaac ggcttcgccg cggccttatc tcttccctgt taagctcctt
     2041 cctggtatct cccaggaaat ttccgccccg cctgtaagcc ggcaccgctc gccgcagccg
     2101 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa tatatcctgt actacatatc
     2161 ctgctgacgc gctggtgccg ccttttttct cctgctacat gaagcacttc actgatttcc
     2221 acatccgtgc caacatagtc agccagtata cactccgcta gcgctacgtg actggttcag
     2281 ggctgcgccc cgaaaccggc taaaagctaa tgacgcgcct gcggcttgtc caacgcctca
     2341 cggcaccgga ttaaaatccg ctcccgttca ccgtttctga ctgcctttca ccgtcgctgg
     2401 cgcttcatcg tttctactgt gtctggcaga ctgtgctgat ttattctgac tggagaacgt
     2461 tatggccaga ctgacaaact tgacgcctgc agagaaaaaa tttcttgatg atgctgttgc
     2521 cgcagctgaa agagcgtcag gtaaaaagct aaatcagcct aaccggcaca tcgtcctgaa
     2581 cagagcgcgt gcgcaaattg agttgcagcg ttacgccgac cgccagcgtg cattacgtga
     2641 ggatgagcgg cagcagtccg attttgcctg gtcacgtccg cgcgcgccgc gccgctgatt
     2701 ttggcttttg gcggtttagt ggtgctgacg ctgcgaaatc acctgccatt cacctgcgtg
     2761 catagccatg cagtcgcgca gaaatcatca ggggcgtttt tccgggatac tggtgaggaa
     2821 aggtgc
//
