LOCUS       MF554638                7725 bp    DNA     circular ENV 20-DEC-2017
DEFINITION  uncultured bacterium clone AA-101 plasmid pFECG, complete sequence.
ACCESSION   MF554638
VERSION     MF554638.1
KEYWORDS    ENV.
SOURCE      uncultured bacterium
  ORGANISM  uncultured bacterium
            Bacteria; environmental samples.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Ajayi,A.O. and Yost,C.K.
  TITLE     Characterization of antibiotic resistance plasmids extracted from
            three waste water treatment plants reveals the diversity of mobile
            elements carrying ciprofloxacin and meropenem resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Ajayi,A.O. and Yost,C.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JUL-2017) Department of Biology, University of Regina,
            3737 Wascana Parkway, Regina, Saskatchewan S4S 0A2, Canada
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: SPAdes v. 3.10.1
            Sequencing Technology :: Illumina; Sanger dideoxy sequencing
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="uncultured bacterium"
                     /mol_type="genomic DNA"
                     /isolation_source="Greenwood waste water treatment plant"
                     /db_xref="taxon:77133"
                     /clone="AA-101"
                     /environmental_sample=""
                     /plasmid="pFECG"
                     /geo_loc_name="Canada"
     CDS             96..869
                     /codon_start=1
                     /transl_table=11
                     /product="phage replication protein"
                     /protein_id="AUF80010.1"
                     /translation="MVHDYGDGKRIEFSGPEPLGADDLRILQGLVAMAGPGGLVLSPEP
                     QTEAGQQLRLFLEPKWEAFQQDAMVVKGSYRALAREIGYANPDDTKPVRECIERLWKVS
                     IIAQNGRKRQGFRLLSEYASDEQDGKLYVALNPLIARAVMGGAQHVRIDMDEVRALQTD
                     PARLMHQRLCGWIDPGKSGRVELDTLCGYVWPGQATNPNTLKTRRQAARRALPELAALG
                     WAVSEYAKNKFEIGRPKATHLLGPAPKTQEISHEK"
                     /name="phage replication protein"
     CDS             1693..2376
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase/ mobilization nuclease"
                     /protein_id="AUF80015.1"
                     /translation="MTKLETVRALNDSGPQLQIVESVRTLQQSLSLMHEDLKRLPVAVS
                     TETAQTLEPLARLQQDVTQVLEAYDKVTAIQRKTLDELTQQMSASAAKAFEQKAGELDK
                     TISDLSNSLSGLETSISSMKQTAEQVADLPGNLVSAQKDMTQAANKLTEAANETRPRLW
                     RQALGLILAGAVGAMLVATGQVALNRLVPPSDVQQTADWANAIWNKATPKERELLEQIV
                     NRPAN"
                     /name="topoisomerase/ mobilization nuclease"
     CDS             2412..3362
                     /codon_start=1
                     /transl_table=11
                     /product="mobA"
                     /protein_id="AUF80014.1"
                     /translation="MKREGRHLSVKAGGDEAYRVTRQQDGRWLWCDRYGNDGGDNIDLV
                     REIEPGTGYAEAVYRLSGAPTVRQQPRPSEPKRQPPQLPAQGLAAREHGRDYLKGRGIS
                     PDTIEHAEKAGMVRYADGGVLFVGYDRTGTAQNATRRAIAPADPVQKRDLRGSDKIYPP
                     ILPGDPAKVWIVEGGPDALALHDIAKRSGQQPPTVIVSGGANVRSFLERDDVQAILKRA
                     ERVTVAGENEKNPEAQAKADAGHQKQAQRVAEITGREVRQWTPKPEHGKDLADMNARQV
                     AEIERKRQAEIEAERARNRELSRKSRGYDGPSFGR"
                     /name="mobA"
     CDS             3433..4536
                     /codon_start=1
                     /transl_table=11
                     /product="recA-family ATPase repA"
                     /protein_id="AUF80011.1"
                     /translation="MAINIMEAFQQEPPQLDFIWPGFLAGTVGALVAPGATGKSFWALE
                     AAMSIACSVAGGDVVNLKPQHTGRVVYLAGEDPEPALIRRVHAIGQHLNQSAREAIAEN
                     LTLEPIMGKRLNVMDEEHLRRVIEYSAGARLIVLDTLSRIHSLDENSNGDMARLVATLE
                     HVAASTGASVLYLHHVSKGSAREGQTDQQQAARGASALIDNARWCGFVAKMTEDEAKRL
                     SDRAYDRQPIGNDRRGFFVRFGVSKQNYDATPLDQWYERRDGGVLVPVELLEVPNGKKG
                     GSREQACWETPPTVEVRIPVKLNSDSGICEHHFRKVCGQGFFEQKLGFEGRHLFDSLNK
                     PAAKRSAAVVDPRRWNRTVHLTSEYVL"
                     /name="recA-family ATPase repA"
     CDS             complement(4944..5600)
                     /codon_start=1
                     /transl_table=11
                     /product="qnrS2"
                     /protein_id="AUF80012.1"
                     /translation="METYRHTYRHHSFSHQDLSDITFTACTFIRCDFRRANLRDATFIN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIELRECDLKGANFSRANF
                     ANQVSNRMYFCSAFITGCNLSYANMERVCLEKCELFENRWIGTHLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIASWQQEQLLEALGIVVFPD"
                     /name="qnrS2"
     CDS             complement(6437..6904)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="AUF80013.1"
                     /translation="MQFKRQGRRVQVLAYRGYDKEKRRAIVKMMGSIDVYSYEPSGGLI
                     ENLTDEEKTELQSYIETERQAAEKRSRVYYAKSAASRIVEVADTIKAGDFEPSEAWAAD
                     TWAAIEALTKAMRKAGYPKLRKAPQKAADAPMPGQAGLPFGDAPETPESAS"
                     /name="hypothetical protein"
     ARG             complement(4948..5600)
                     /name="qnrS2"
                     /gene="qnrS2"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS2"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          3434..4295
                     /name="IncQ2"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.494"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          922..3359
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.401"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acctgaccca cgcccggcac gacccggcgc attgtctggc ccccggccta ttccgcagcc
       61 tcaagcgtgg ccagcgcaag cgcggcaagc tggacgtggt gcatgactat ggcgacggta
      121 agcgcatcga gttcagcggc ccggagccgc tgggcgctga tgacttgcgc atcctgcaag
      181 gacttgtggc aatggctggg ccgggtggcc ttgtgctgtc acctgagccg cagaccgagg
      241 ccgggcagca actccggctg ttccttgagc cgaaatggga agccttccag caggatgcga
      301 tggtggtcaa gggcagctat cgagcacttg cccgcgaaat tggctacgcc aaccccgacg
      361 acactaagcc ggttcgggag tgcatcgagc ggctttggaa ggtgtccatc atcgcccaga
      421 acggcaggaa gcggcagggc ttccgcctgc tgtccgagta cgccagcgac gagcaggacg
      481 gaaagctgta tgtagccttg aatcccctga ttgctcgcgc cgtcatgggt ggcgcgcagc
      541 atgtccgcat cgacatggac gaggtgcggg cgctacagac cgacccggcg cggctgatgc
      601 accagcgcct atgcggctgg atcgaccccg gcaagtccgg gcgggtggaa ctggacacgc
      661 tttgcggcta cgtctggccg gggcaggcaa ccaaccccaa tacgctcaag acgcgacgcc
      721 aagcggctcg cagggcgctt cctgagcttg ccgcattggg ctgggcagtc agcgaatacg
      781 caaaaaacaa gtttgaaatc ggcaggccga aagcaacgca tctgctcggc ccagcaccca
      841 aaacacagga aattagccat gagaaatgat ggtgcgcggc gacttgatag gcgtcaagca
      901 cgactcggag gacgtggaag atgaaagcga aggttagccg tggtggcggt tttcgcggtg
      961 cgctgaacta cgtttttgac gttggcaagg aagccacgca cacgaaaaac gcggaacgtg
     1021 tcggcggcaa catggccggg aatgaccccc gcgaactgtc gcgggagttc tcagccgtgc
     1081 gccagttgcg cccggacatc ggcaagcccg tctggcattg ctcgctgtca ctgcctcccg
     1141 gtgagcgcct gagcgccgag aagtgggaag ccgtgacgac tgacttcatg cagcgcatgg
     1201 gctttgacca gaccaatacg ccgtgggtgg cagtgcgcca ccaggacacg gacaaggatc
     1261 acatccacat cgtggccagc cgggtagggc tggacgggaa agtttggctg ggccagtggg
     1321 aagcccgcag cgccatcgag gcgacccaag agcttgagca tacccacggc ctgaccctga
     1381 cgccggggct gggcgatgcg cgggccgagc gccggaagct gaccgacaag gagatcaaca
     1441 tggccgtgag aacgggcgac gaaccgccgc gccagcgtct gcaacggctg ctggatgagg
     1501 cagtgaagga caagccgacc gcgccagaac tggccgaccg gctacatgcc gcaggcgtag
     1561 gcgtccgggc aaacctcgcc agcaccgggc gcatgaacgg cttttccttc gaggtggccg
     1621 gagtgccgtt caagggcagc gacttgggca agggctacac atgggcggga ctacagaaat
     1681 caggggtgac ttatgacgaa gctagagacc gtgcgggcct tgaacgattc aggcccacag
     1741 ttgcagatcg tggagagcgt caggacgttg cagcagtccc tgagcctgat gcacgaggac
     1801 ttgaagcgcc taccggtcgc agtctcgacc gagacggcgc agaccttgga accgctggcc
     1861 cgactccaac aggacgtgac gcaggttctg gaagcctacg acaaggtgac ggccattcag
     1921 cgcaagacgc tggacgagct gacgcagcag atgagcgcga gcgcggcgaa ggcgttcgag
     1981 cagaaggccg gggagctgga caagaccatc tccgacctgt ccaacagcct gtcagggctg
     2041 gaaacgagca tcagcagcat gaagcagacc gcggagcagg tggcggactt gccgggcaat
     2101 ctggtgagcg cacagaagga catgacgcaa gccgccaaca aactgaccga ggcagcgaac
     2161 gagacgcgcc cgcgcctttg gcggcaggcg ctggggctga ttctggccgg ggccgtgggc
     2221 gcgatgctgg tagcgactgg gcaagtcgct ttaaacaggc tagtgccgcc aagcgacgtg
     2281 cagcagacgg cggattgggc caacgcgatt tggaacaagg ccacgcccaa ggagcgcgag
     2341 ttgctggaac agatcgtcaa tcggcccgcg aactagatcc gaccgcctac cttgaggcga
     2401 gcggctacac cgtgaagcgc gaagggcgac acctgtccgt caaggctggc ggtgatgagg
     2461 cgtaccgcgt gacccggcag caggacgggc gctggctctg gtgcgaccgc tacggcaacg
     2521 acggcgggga caatatcgac cttgtgcgcg agatcgaacc tggcaccggc tacgccgagg
     2581 ccgtctatcg gctttcaggt gcgccgacag tccggcagca accgcgcccg agcgagccga
     2641 aacgccaacc gccgcagcta ccggcgcaag ggctggcagc ccgcgagcat ggccgcgact
     2701 acctcaaggg ccggggcatc agcccggaca ccatcgagca cgccgagaag gcgggcatgg
     2761 tgcgctatgc agacggtggc gtgctgttcg tcggctacga ccgtacaggc accgcgcaga
     2821 acgccacacg ccgcgccatt gcccccgctg atccggtgca gaagcgcgac ttacgcggca
     2881 gcgacaagat ctatccgccg atcctgccgg gcgacccggc aaaggtctgg atcgtggaag
     2941 gtggcccgga tgcgctagcc ctgcacgaca tcgccaagcg cagcggccag cagccgccca
     3001 ccgtcatcgt gtcaggcggc gcgaacgtgc gcagcttctt ggagcgtgac gacgtgcaag
     3061 cgatcctgaa gcgggccgag cgcgtcactg tggccgggga aaacgaaaag aaccccgagg
     3121 cgcaggccaa ggccgacgcc gggcaccaga aacaggcgca gcgggtggcc gaaatcaccg
     3181 ggcgcgaggt gcgccaatgg acgccgaagc ccgagcatgg caaagacttg gccgacatga
     3241 acgcccggca ggtggcagag atcgagcgca agcgacaggc cgagatcgag gctgagagag
     3301 cgcgaaatcg agagctttca cgcaagagcc gggggtatga tggccccagc ttcggcagat
     3361 aagagcgcct gtcagtcggt gtgatgaacc gaccgacagg ctaaccatca tcaacctgag
     3421 taggaggccg atatggctat caacattatg gaggcgttcc agcaggaacc gccacaactg
     3481 gacttcatct ggcccggctt tctggctggc accgtgggcg cactggtggc ccccggcgcg
     3541 accggcaaga gcttctgggc gctggaagcg gctatgtcca tcgcttgcag cgtggcaggc
     3601 ggtgacgtgg tgaacctcaa gccgcagcac accgggcgcg tggtctatct ggccggtgaa
     3661 gatcccgaac ccgccctgat ccggcgcgtt cacgccatcg gccagcacct caaccagtca
     3721 gcccgcgaag ccatcgccga gaacctgacc cttgagccga tcatgggcaa gcgcctgaac
     3781 gtcatggacg aggagcactt gcgccgcgtc atcgagtaca gcgccggggc gcggctgatc
     3841 gtgctggaca ccctgagccg gattcacagc ctcgacgaga acagcaacgg cgacatggcg
     3901 cggcttgtcg ccacgctgga acacgtcgcg gccagcactg gcgcgtctgt cctgtacctg
     3961 caccacgtca gcaagggcag cgcccgcgag gggcaaaccg accagcagca ggcagcgcgg
     4021 ggcgcatcgg cactgatcga caacgcccgc tggtgcggct tcgtcgccaa gatgaccgag
     4081 gacgaggcca agcgcctgag cgaccgcgcc tatgaccgtc agcccatcgg caacgaccgg
     4141 cgcggcttct tcgtgcgctt cggcgtgagc aaacagaact acgacgccac cccgcttgac
     4201 cagtggtatg agcgccgaga cggcggcgtg ctggttccgg tggaactgct ggaagtgccg
     4261 aacggcaaga aggggggcag ccgtgagcaa gcatgttggg aaacgccgcc gacagtcgaa
     4321 gtgcgtattc cggtgaagtt gaacagtgat tccggaatct gtgaacacca tttccggaaa
     4381 gtatgcggac agggattctt cgagcagaag ctgggctttg aggggaggca tcttttcgac
     4441 tcactgaaca agccagcagc caagcgcagc gcggcagttg tggatccaag acgttggaac
     4501 aggacggttc atctgacatc agagtacgtg ctctgaggtg ttgtaggaga ggaataaagt
     4561 ttcatactaa gcagtcaagt aaccctgttt gtaccgttct aaatgatact cacccagaaa
     4621 taaagattca tactaattcc tactacaaaa tctgagtcac gctacaacat actaagcagt
     4681 caagtaaccc tgtttgtacc gttctaaatg atactcaccc agaaataaag attcatacta
     4741 attcctacta caaaatctga gtcacgctac aactgagtaa taaagtatca tactggttgt
     4801 cttgagcatc cacagattga ttggccccat tcaaacacgt ttctggacaa gaatgagcta
     4861 gaagtattga tctagctctg gtgatttgaa ttagaaaatg tattaatgtt gtgtatagtt
     4921 tatcagcagg tattcgtaag catctagtca ggaaaaacaa caatacccaa cgcttcgaga
     4981 agctgttctt gttgccagct ggcaattttg atacctgatg tatcgacttt tcgaggatct
     5041 aaaccgtcga gttcggcgtg acataaatta gcaccctgta ggctaaactg tccccagaca
     5101 tcttcagaaa aaacacctcg acttaagtct gactctttca gtgatgcgcc cgcgaggtga
     5161 gtccctatcc agcgattttc aaacagctca catttttcta aacagacccg ctccatattg
     5221 gcataagaca ggttacatcc agtaataaag gctgagcaaa agtacatacg attactcact
     5281 tgattggcaa agtttgctcg ggaaaagttg gcccctttta aatcacactc acgtaactca
     5341 ataccgtagc aattggcgtt actaaagttt gccatcgcaa gctggcattg ttggaaactt
     5401 gcatcgcgaa ggtctgcgac atcaaaatgg caaccttcga tatcaccctg ttcaatgaac
     5461 ttgcagttaa taaatgtcgc atcacgcaag ttagcacgtc gaaaatcgca tcggataaag
     5521 gtgcaagcag tgaaagtaat atcacttaga tcttgatgtg aaaaactgtg gtgtcgatat
     5581 gtgtgacggt aggtttccat gttgactcct atgatgaact ggacgcaaca ctaccatcaa
     5641 agccacatca catcaccaaa ataaaaacac taaaaatcaa gatcttaaaa accctcattt
     5701 tgcctatttt aagggggtaa aatagcgccc tgagcctcta tgttaacgtt caaagtacgc
     5761 ggcaccattg aaaaaagtta tccgcttaaa aagccacaga gaaaaggaac ccaatagatt
     5821 actgatgtcc gccataggta aagtaacctt aaacggaagc ctgcaatttg aagtagatac
     5881 aaggtctaag aaggaatggt tggctgtttt attttattgt actcggcatt aagcccaatt
     5941 tctttagcgt tacgataagc tagcatggct acaccgaccc cgctaggctg attgggaagc
     6001 gttgcctgat ctactcagga gggcgcttcg ataacgtagc cattgagccg gaaaccggcg
     6061 agcactgggg cagcgtgatc tggtgcgagt atgtgtgacc ccctgtttaa ctccacccaa
     6121 cccccacggt aactccaccc aacccccacg gtaactccac ccaaccccca cggtaactcc
     6181 acccgccccg atctggaaaa cccagacggg gcgcggattt gctggcgatt cgcaaaatcc
     6241 atccaagatt ctctaagaat ctccactagg cgcagcacag cccgcgcccc ttcggcgcgg
     6301 tctggcattg cctcaaatgc ccggccaaga ccatgcgcaa ggcgaacacc tctccccccg
     6361 ccttcggcga ccccctccgg gggtaaaggg ccagcgggga gagggggggg ggtgccggtt
     6421 tcccggccta aacccctcag gaggcgcttt ctggcgtttc tggcgcatcc ccaaagggca
     6481 accccgcctg acccggcatc ggcgcgtctg cggctttctg gggcgctttg cggagcttcg
     6541 ggtagcccgc cttgcgcatg gccttggtca gcgcctcgat tgccgcccag gtatccgccg
     6601 cccatgcctc ggacggctca aagtccccgg ccttgatcgt gtcggcaacc tcaacaatac
     6661 gactggcggc agacttagcg taatacactc ggcttctctt ttctgctgcc tgtcgttctg
     6721 tttcgatgta tgactgtagt tctgtttttt cttcgtctgt cagattctca attaggccgc
     6781 ctgacggctc gtaagaatat acgtcaatag accccatcat cttgacgatg gcgcggcgtt
     6841 tttccttgtc atacccccgg taggcgagca cctgaacccg gcgaccttgg cgcttaaact
     6901 gcatggcggt ttctccgttt gactgtgtga ttatattata gttcgacagt cgcacgctgt
     6961 cgagtgttta ttgtcggcct atttttcaat ccgaccgtcg cactcaaata ggtttgcact
     7021 gtcggattga gtaacgctcg acagtctgcc gttcttgcga taccatagtt gcattgtatc
     7081 agttcgcccc ggcgccttgc ccaccccctc tccccgctgg cccattaccg cccttgggcg
     7141 gtcgggccga gagcgcagcg agcgggccgg gtgggtggct gttcggtgct tgcatggaga
     7201 tgtgctatta tggtatttgc gcggtggttt ggcatggagc aaggtgtaac ctacgcttcg
     7261 cttggttctc accctccggc cttcggccta cgcccccgct cccttcggtc ggggggttgc
     7321 tgcgcgtttc gcttcgcttc acttactgga gggcttagcc catgaacact gacgaatcca
     7381 agcgccgccg ccgtgggcct gcgccgctcg acgccacgga caagcgcggg cataccgttt
     7441 ctgtgcgcct caacgaggcc gagcttgcgc ggctggattc gcagcgtgac gcggtgcaga
     7501 tgcagcgggg cgagtaccta cgcgccgccg ctctgcatcg cctgccgccc accatcccgg
     7561 cagcgaaccg ggaacagtgg gcagagttgg cccgcacagc ggccaacctg aaccagatcg
     7621 cccggcacct gaacgaaggg cagcggggcg acggggagcg catcggcaag gcgctgggct
     7681 tgcagttgag cgccgagctt gccaactgcc tgcgattgct ggttc
//
