LOCUS       LC807778               10494 bp    DNA     circular BCT 04-APR-2024
DEFINITION  Escherichia coli EC12 plasmid pEC12-NDM-5 DNA, complete sequence.
ACCESSION   LC807778
VERSION     LC807778.1
DBLINK      BioProject: PRJDB15848
            BioSample: SAMD00607277
            Sequence Read Archive: DRR466439, DRR466477
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Nisa,T.T., Sugawara,Y., Hamaguchi,S., Takeuchi,D., Abe,R.,
            Kuroda,E., Morita,M., Ueda,A., Nishi,I., Hossain,N., Hasan,M.M.,
            Siddiqee,M.H., Nakatani,D., Nakata,K. and Akeda,Y.
  TITLE     Genomic characterization of carbapenemase-producing Enterobacterales
            from Dhaka food markets unveiled the spread of high-risk
            antimicrobial resistant clones and plasmids co-carrying blaNDM and
            mcr-1.1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Sugawara,Y. and Akeda,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2024) Contact:Yo Sugawara National Institute of
            Infectious Disease, Antimicrobial Resistance Research Center; 4-2-1,
            Aoba-cho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.5.0
            Genome Coverage       :: 502x
            Sequencing Technology :: MinION; Illumina NovaSeq 6000
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC12"
                     /isolation_source="betel leaf"
                     /db_xref="taxon:562"
                     /plasmid="pEC12-NDM-5"
                     /geo_loc_name="Bangladesh:Dhaka"
                     /collection_date="2021"
     ARG             complement(6342..6685)
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(5509..6344)
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             381..1189
                     /name="blaNDM-5"
                     /gene="blaNDM-5"
                     /gene_name="subclass B1 metallo-beta-lactamase NDM-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6853..7640)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(8052..8545)
                     /name="dfrA12"
                     /gene="dfrA12"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1197..1558
                     /name="ble"
                     /gene="ble"
                     /gene_name="bleomycin binding protein Ble-MBL"
                     /tool="amrfinderplus"
                     /drug_class="BLEOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6850..7628)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1197..1561
                     /name="BRP(MBL)"
                     /gene="BRP(MBL)"
                     /gene_name="Bleomycin resistant protein"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 cataaaatac tcgaagaaga tttaggcata gatgtatatt tctgtgaccc acattcaccc
       61 tggcaaaaag gcacatgcga aaatatgaat ggtttaatta ggcaatattt acctaaaggg
      121 attgatttaa atcaggcaga tcagcattat ttaaatcaag ttgccatgtc actgaatact
      181 cgtcctagaa aggcgttaga ttggcttaca ccattagaga aatttgctca gcttgttgat
      241 tatcatatgg cttttgaaac tgtcgcacct catgtttgaa ttcgccccat atttttgcta
      301 cagtgaacca aattaagatc atctatttac taggcctcgc atttgcgggg tttttaatgc
      361 tgaataaaag gaaaacttga tggaattgcc caatattatg cacccggtcg cgaagctgag
      421 caccgcatta gccgctgcat tgatgctgag cgggtgcatg cccggtgaaa tccgcccgac
      481 gattggccag caaatggaaa ctggcgacca acggtttggc gatctggttt tccgccagct
      541 cgcaccgaat gtctggcagc acacttccta tctcgacatg ccgggtttcg gggcagtcgc
      601 ttccaacggt ttgatcgtca gggatggcgg ccgcgtgctg ttggtcgata ccgcctggac
      661 cgatgaccag accgcccaga tcctcaactg gatcaagcag gagatcaacc tgccggtcgc
      721 gctggcggtg gtgactcacg cgcatcagga caagatgggc ggtatggacg cgctgcatgc
      781 ggcggggatt gcgacttatg ccaatgcgtt gtcgaaccag cttgccccgc aagaggggct
      841 ggttgcggcg caacacagcc tgactttcgc cgccaatggc tgggtcgaac cagcaaccgc
      901 gcccaacttt ggcccgctca aggtatttta ccccggcccc ggccacacca gtgacaatat
      961 caccgttggg atcgacggca ccgacatcgc ttttggtggc tgcctgatca aggacagcaa
     1021 ggccaagtcg ctcggcaatc tcggtgatgc cgacactgag cactacgccg cgtcagcgcg
     1081 cgcgtttggt gcggcgttcc ccaaggccag catgatcgtg atgagccatt ccgcccccga
     1141 tagccgcgcc gcaatcactc atacggcccg catggccgac aagctgcgct gagccatggc
     1201 tgaccacgtc acccccaatc tgccatcgcg cgatttcgat gtgacagagg cgttttatgc
     1261 gaagctgggc tttgcgacga gttggaagga tcgcggctgg atgatcctgc agcgcggcgg
     1321 tttgcagctc gaattcttcc cctatcctga cctcgaccca gctacgagct cgttcggctg
     1381 ttgcctgcgg ttggatgatc tcgatgccat ggtggcattg gtgaacgcgg cgggagccga
     1441 ggaaaaaagc accggctggc cgcgcttcaa agctccgcaa ctggaggcga gcggcctgag
     1501 gatcggctac ctgatcgatc ccgactgcac gctggtgcgg ctgatccaga accccgactg
     1561 accgcatgcc cgcgaaaatc aagatttgcg ggatcagcac acccgaggcg ctcgatgcga
     1621 ccatcgcggc gcgggcggac tatgccgggt tggtgttcta tccagcgtcg ccccgtgcgg
     1681 ttacgtcgaa tgtcgcgggc gctttgacat cgcgcgcagc tggccagatc gccatggtcg
     1741 gtttgttcgt cgatgcggat gatgctgtca tcgccgacgc actggtggca gccaagctga
     1801 acgcgctgca gctgcacggt tcggaatcgc ccgaacgcgt ggcccagttg cgcgcgcggt
     1861 ttggcaagcc ggtgtggaag gcgctgcccg tcgccagcgc cagcgatgtc gcacgcgccg
     1921 cagcctatgc cggggcggcg gacttgatct tgttcgacgc caagaccccc aaaggcgcgc
     1981 tgcccggcgg catggggttg gcgttcgact ggtcgctgct ggccggatat cgcggtgcct
     2041 tgccgtgggg gctggcaggc gggctaaatc cgacgaatgt tgccgaggcg attgcgcgca
     2101 ccggagcgcc gctggtcgat acctccagcg gcgtcgaaag cgcgccgggc gtcaaggata
     2161 ccgacaagat taccaatttc gcctttgcgg tgcgcttggc ctaaatcgcg tcgatcaata
     2221 ggcgtcgttc agcgcaaaga tcggcttgcg ggtgcgccac tgccctcggg tgaagtcggg
     2281 aaaatctaac gtgcgattgc cctcagcaat cgattgttcc gacagaggcg tgatcgcgct
     2341 ccaggccagc gcgtcgtaaa tgtcgattgg catcggggcc ttggccttca gcgcctcgac
     2401 aaaagcgtgg atcacgaacc agtccatccc gccatgcccg gcccctgccg ccagatcggc
     2461 gtagcgtttc catagcgggt gatcgtattt cgcaaaccag ccctcggcag gctcccagcg
     2521 gtgcggctgt gggctcttgc cctccagata gatcgacttg ttgacgtcca tccacagccc
     2581 ctcggtgcct tgcacccgaa agccgagaga ataggggcgc ggcagcgagg tgtcgtggca
     2641 cagcatgatc gtttcaccat tagtgcagcc gatcatggtg ttgaccacat cacccagtgc
     2701 gaatttcacc tcggcgttgg gatgatcggc agagccgttc ttgacgacat aatcatgcag
     2761 cccgcgcgcc ttacagccga agccgccagc gcccgcttcg cccggcaacg cgaccttcag
     2821 ggtgcgggtc tgcggcgggt agcacacgcc ggcatcggcg cagccctggt acttcacggt
     2881 cagggtggtc gcgctcgcgc cggccgcggg cgtgccggtg agggtgccga gcaattcctt
     2941 gcggtaggtt tcgacgtcgc cgaagaattc gtcgcggtag gccttgccct tcggcagcgc
     3001 catggtcgcg ccggtgaagg cggcatcggc cttgaccgag gtgcggtgcc ggtacaggta
     3061 atagccgtcg gcgatccgcc agcgcacctc gatgcggtcc ggcgcggtgg cctgcgcgga
     3121 caggacgaag acctcgtcga ccggcggcag ttcgaagtcc tgggcgacgg ccgaggtcgc
     3181 gggcagcgca agcagcaggg cgagcccggc cagccagcgg cgcaggcgga tcgtggatgc
     3241 ggtcattggc tcagtttacc ggtcggctct cggcggccag ccattgcagg tattcgggca
     3301 ggccggacgc ggcttcgacc gcgagcgggt ataggaagta taaaccacct ttttgctcct
     3361 catccgaagt atcttacctg aaattccctc actcgtttac cgctcaagcc ccaattttaa
     3421 ctgccggtcc agcctaaacc gctctaataa ggttcgattt ggcggtaaaa tctctagcct
     3481 gatagctcga gagatacaaa ctgccccacc gccccgttta aaagttggca gtgttgagca
     3541 gtgttggatt tggggtcgtc agtcaaagag acgactctgt gatggatcga acaggctggg
     3601 agtcagtggc ggcgctcgtt ctggtggcag ctcacgctgc ttggcggcat tcgccttggc
     3661 tgttttctgt ttcagatgct tgagaatctg ctcaatgacc ttcggatctt cgatgctggc
     3721 aatcactttg acgtgaccgc cgcagtgttc gcagacttca atatcaatat tgaagactcg
     3781 cttgaggcgt tgcatccagg tcatggcgcg gtggcgctct gcaggactct tgtcacgcca
     3841 gttagtatcg agaccttccg atttgtcggg cttcttgccc cgcttggcgg gtgttacttg
     3901 aactcggtgt ttgctgttcg gtgcaaagac gccgtggaag cgtgtgaggt tgactcgcgg
     3961 cttaggtacc aacgcagcga gtttggcgat gaagtccagc ggctcgaaga tcacatgggt
     4021 ggtgccattg cggtacggag ttttgagctc gtaacgcacc tgcccattgg cggttaatgc
     4081 cagacgtttt tctgaaaccg ctggccgact aatgtagcga cacaagcgct caagcttatc
     4141 ccgctgatgc gcttcggcca tcacaccggc gtgtagcgag aaaccagcat ggttggctac
     4201 tcgactgctt gagtcggctt tatcctcacg ccctggcaag gtttgcaggg tgaagacttt
     4261 gcgcccttgc tgggggccga cggcaatgcg atacgtaacc gaagcaccat gtaattgagt
     4321 cagcgtatcg tcttcgccct cttccagtgt caaccacgta ttctcggcat cacgctccaa
     4381 aatcccacgc ttttccatgc agcgagcgat gcgatggctg agggtgtgag cgagcgtatt
     4441 cagctcatcg taagtgggtg ccttgacacg atggaagcgt tgcttgccat agtcatcttc
     4501 ggcatagaca ccatcgagaa acagcatgtg gtagtggaca ttgagattta gcgcggagcc
     4561 aaagcgttgg ataagagtca ctgagccagt ttgtgcagag gctttggtgt aaccggcttt
     4621 tttgatcaga tgagttgaga gtgtacgata gacgatactc aagacctggc ccatcagctg
     4681 gggatggcga gccagcaaaa agcgtagctg gaaaggaaag ctgagcaccc actggcgaat
     4741 gggctccttg gggaagactt cgtctatcag cagcgccgca ctctcggcca tccggcgggc
     4801 accgcagcta gggcaaaagc cgcgtcgttt acagctgaag gcgaccagac gctcgtgatg
     4861 acaatcctcg cagcgaaccc gcatgaaacc atactccaga cggccacatt ggaggaggtc
     4921 gttgaattct tgttggatgt agcgaggcag gtgttgacct tgggcttcga gtgaggcttt
     4981 gaaggctggg tagtgctgct caaccagctg gtagagcagc gtctggtcgg gttggtggcg
     5041 ttcgtaaccg tttgtttgag tgggcgattg actcgccgtg gcgttccttg ccagcgacat
     5101 gggtatcctc cgctgatact gtggttatgt acagtatcag cggcttgcgt tcagacgtcc
     5161 agtctggccc tagacatcgc taaatgctta acccgcaata gccctcacga gttgttatca
     5221 gccactaccg gttgagcgag aaggttttgg gttcagggtg ctattgctcc accaatcaca
     5281 atactgaagc cccaactgtt atcagttggg gctttttctt gtctgtttgc ggcggttgcg
     5341 ttttatcggt agtcgtcgag ctctgcacca tcccacataa gagcttaacg gtgcgatctt
     5401 caacgccatc acacaaaact ttctttttca cgcacagtca acttattgga tgttttatta
     5461 acaacccaaa aggagatatt tagcgggcgg ccggaaggtg aatgctaggc atgatctaac
     5521 cctcggtctc tggcgtcgcg actgcgaaat ttcgcgaggg tttccgagaa ggtgattgcg
     5581 cttcgcagat ctccaggcgc gtgggtgcgg acgtagtcag cgccattgcc gatcgcgtga
     5641 agttccgccg caaggctcgc tggacccaga tcctttacag gaaggccaac ggtggcgccc
     5701 aagaaggatt tccgcgacac cgagaccaat agcggaagcc ccaacgccga cttcagcttt
     5761 tgaaggttcg acagcacgtg cagcgatgtt tccggtgcgg ggctcaagaa aaatcccatc
     5821 cccggatcga ggatgagccg gtcggcagcg accccgctcc gtcgcaaggc ggaaacccgc
     5881 gcctcgaaga accgcacaat ctcgtcgagc gcgtcttcgg gtcgaaggtg accggtgcgg
     5941 gtggcgatgc catcccgctg cgctgagtgc ataaccacca gcctgcagtc cgcctcagca
     6001 atatcgggat agagcgcagg gtcaggaaat ccttggatat cgttcaggta gcccacgccg
     6061 cgcttgagcg catagcgctg ggtttccggt tggaagctgt cgattgaaac acggtgcatc
     6121 tgatcggaca gggcgtctaa gagcggcgca atacgtctga tctcatcggc cggcgataca
     6181 ggcctcgcgt ccggatggct ggcggccggt ccgacatcca cgacgtctga tccgactcgc
     6241 agcatttcga tcgccgcggt gacagcgccg gcggggtcta gccgccggct ctcatcgaag
     6301 aaggagtcct cggtgagatt cagaatgccg aacaccgtca ccatggcgtc ggcctccgca
     6361 gcgacttcca cgatggggat cgggcgagca aaaaggcagc aattatgagc cccataccta
     6421 caaagcccca cgcatcaagc ttttgcccat gaagcaacca ggcaatggct gtaattatga
     6481 cgacgccgag tcccgaccag actgcataag caacaccgac agggatggat ttcagaacca
     6541 gagaaagaaa ataaaatgcg atgccataac cgattatgac aacggcggaa ggggcaagct
     6601 tagtaaagcc ctcgctagat tttaatgcgg atgttgcgat tacttcgcca actattgcga
     6661 taacaagaaa aagccagcct ttcatgatat atctcccaat ttgtgtaggg cttattatgc
     6721 acgcttaaaa ataataaaag cagacttgac ctgatagttt ggctgtgagc aattatgtgc
     6781 ttagtgcatc taacgccgga gttaagccgc cgcgcgtagc gcggtcggct tgaacgaatt
     6841 gttagacatc atttaccaac tgacttgatg atctcgcctt tcacaaagcg aataaattct
     6901 tccaagtgat ctgcgcgtga ggccaagtga tcttcttttt gtcccagata agcttgctta
     6961 gcttcaagta agacgggctg atactgggca ggtaggcgtt ttattgccca gtcggcagcg
     7021 acatccttcg gcgcgatttt gccggttatt gcgctgtacc aaatgcggga caacgtaagc
     7081 actacatttc gctcatcgcc ggcccagtcg ggctgcgagt tccatagctt caaggtttcc
     7141 ctcagcgcct cgaatagatc ctgttcagga accgggtcaa agaattcctc cgctgccgga
     7201 cctaccaagg caacgctatg ttctcttgct tttgtaagca ggatagctag atcaatgtcg
     7261 atcatggctg gctcgaagat acccgcaaga atgtcattgc gctgccattc tccaaattgc
     7321 agctcgcgct tagccggata acgccacggg atgatgtcgt catgcacgac aagggtgact
     7381 tctatagcgc ggagcgtctc gctctcgcca gggaaagccg aagcctccat aagatcattg
     7441 agcaatgctc gccgcgtcgt ttcatcaagc tttacggcca cagtaaccaa caaatcaata
     7501 tcgctgtatg gcttcaggcc gccatccact gcggagccgt acaaatgcac ggccagcaac
     7561 gttgattcca gatggcgctc aatgacgctt agcacctctg atagttggtt cgaaatttcg
     7621 atggtcaccg cttccctcat gatgtctaac gggcgaggta agccgaccgc agaatgcggg
     7681 tcggcttgac cgaaatgtta gaaccagaag ccaaaacgga taacttgaat ttggcgacgg
     7741 gcgctaaccg tgaaaaaacg ctgcgccacc gaggcggcac agcactgcaa aaacgatagc
     7801 tgcttgcgct tgctacgcaa gggctagagg ccaaaaagac tgaaaacctg cgcagcccat
     7861 gcaggcgaag cccggaaaaa aggcaaaaca ggcactgaat aacgcctgaa aagctaaatg
     7921 ccgtttgaat aaacatgagc taaataaagc tgggtttcag tggtgctaac gttggacgta
     7981 acgagagccg gagcgcagcg gagggaacca aaatgcgcag cattttggcg tcccgttgac
     8041 ggaatggtta gccgtttcga cgcgcataaa cggagtgggt gtacggaatt acagcttgaa
     8101 tggtttcggt tgagacaagc tcgaattctg tttcgttgag cattgggaag aaggcgtcac
     8161 cctcgaaggt ttgatgtacc tcagatagaa acacgccgtg ggcgtgaggt agtgccagag
     8221 tgtatatctc agctccgccc gcgacgtaga gttcattgcc gagttcggat gccaaagcga
     8281 tagcgtgcga cagcgttgaa acaactacgc agccagtggc gcggtagtta gcttggcgtg
     8341 agattaccaa tgtgtgacgg ttcggtagag gcttgccgat agactcaaag gtctttcgcc
     8401 ccatgacaac gacttttccc tcagtgagtc tgcgaaaaat cttctgctca cccggaattt
     8461 tccaggggat attaggacca ttgccaataa cccgattggc tcccatcgca gcaacgagat
     8521 aaatgcgtac tgattccgag ttcatatggc taactttgtt ttagggcgac tgccctgctg
     8581 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg
     8641 cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca tgaaaaccgc
     8701 cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc gtgagcgcat
     8761 acgctacttg cattacagct taccaaccga acaggcttat gtccactggg ttcgtgcctt
     8821 catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag tcgaggcatt
     8881 tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc
     8941 ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgccctggc ttcaggagat
     9001 cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg aagtggttcg
     9061 catcctcggt tttctggaag gcgagcatcg tttgttcgcc cagcttctgt atggaacggg
     9121 catgcggatc agtgagggtt tgcaactgcg ggtcaaggat ctggatttcg atcacggcac
     9181 gatcatcgtg cgggagggca agggctccaa ggatcgggcc ttgatgttac ccgagagctt
     9241 ggcacccagc ctgcgcgagc agctgtcgcg tgcacgggca tggtggctga aggaccaggc
     9301 cgagggccgc agcggcgttg cgcttcccga cgcccttgag cggaagtatc cgcgcgccgg
     9361 gcattcctgg ccgtggttct gggtttttgc gcagcacacg cattcgaccg atccacggag
     9421 cggtgtcgtg cgtcgccatc acatgtatga ccagaccttt cagcgcgcct tcaaacgtgc
     9481 cgtagaacaa gcaggcatca cgaagcccgc cacaccgcac accctccgcc actcgttcgc
     9541 gacggccttg ctccgcaggg cactgttgca aagttagcga tgaggcagcc ttttgtctta
     9601 ttcaaaggcc ttacatttca aaaactctgc ttaccaggcg catttcgccc aggggatcac
     9661 cataataaaa tgctgaggcc tggcctttgc gtagtgcacg catcacctca atacctttga
     9721 tggtggcgta agccgtcttc atggatttaa atcccagcgt ggcgccgatt atccgtttca
     9781 gtttgccatg atcgcattca atcacgttgt tccggtactt aatctgtcgg tgttcaacgt
     9841 cagacgggca ccggccttcg cgtttgagca gagcaagcgc gcgaccatag gcgggcgctt
     9901 tatccgtgtt gatgaatcgc gggatctgcc acttcttcac gttgttgagg attttaccca
     9961 gaaaccggta tgcagctttg ctgttacgac gggaggagag ataaaaatcg acagtgcggc
    10021 cccggctgtc gacggcccgg tacagatacg cccagcggcc attgaccttc acgtaggttt
    10081 catccatgtg ccacgggcaa agatcggaag ggttacgcca gtaccagcgc agccgttttt
    10141 ccatttcagg cgcataacgc tgaacccagc ggtaaatcgt ggagtgatcg acattcactc
    10201 cgcgttcagc cagcatctcc tgcagctcac ggtaactgat gccgtatttg cagtaccagc
    10261 gtacggccca cagaatgatg tcacgctgaa aatgccggcc tttgaatggg ttcatgtgca
    10321 gctccatcag caaaagggga tgataagttt atcaccaccg actatttgca acagtgccgt
    10381 ttactcatac ctagattcta cgtcagtact tcaaaaagca taatcaaagc cttgataaat
    10441 atgcattcct tcgaaattca gctttcaccc attgggtgaa agaaaagtgc tcaa
//
