LOCUS       LC745731               17093 bp    DNA     circular BCT 25-JAN-2023
DEFINITION  Escherichia coli JARB-RN-0061 plasmid pJARB-RN-0061_VEB-1 DNA,
            complete genome.
ACCESSION   LC745731
VERSION     LC745731.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Shindou,J., Hayashi,W., Kayama,S., Yu,L., Zuo,H., Sugawara,Y. and
            Sugai,M.
  TITLE     First detection of VEB-1 extended-spectrum beta-lactamase-producing
            Escherichia coli clinical isolate in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17093)
  AUTHORS   Hayashi,W. and Sugai,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2023) Contact:Wataru Hayashi National Institute of
            Infectious Disease, Antimicrobial Resistance Research Center; 4-2-1,
            Aoba-cho, Higashimurayama, Tokyo 189-0002, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8
            Sequencing Technology :: Oxford Nanopore Technologies GridION;
            Illumina HiSeq X Five
            ##Genome-Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="JARB-RN-0061"
                     /serovar="O2:H7"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pJARB-RN-0061_VEB-1"
                     /geo_loc_name="Japan: Tokyo"
                     /collection_date="2021"
     gene            complement(173..1903)
                     /gene="mobA"
     CDS             complement(173..1903)
                     /gene="mobA"
                     /codon_start=1
                     /transl_table=11
                     /product="MobA relaxase"
                     /protein_id="BDV50522.1"
                     /translation="MKVGKAGKAGPHAAYIAREGQYANRLERGEKLEATEAGNMPAWAQ
                     SNPLAFWQAADAYERKNGTTYREMEIALPRELSAAQRIELVREFVRQEIGDRHAYQWAL
                     HVPTAADGGEQPHFHLMFSERQVDGIDRDPEQYFKRYNAKAPEKGGARKGYGPSAGQTL
                     TKAERAAELKELRGRWEAMCNAHLERAGVEQRIDMRSHAERGTGLEPERKQLPSAWRGE
                     GRAQVIEFRAARAEVAQAAAELRRAVPDTGAEIIHLEAERQRREQAQREARERAEREKA
                     QQERQRLERMSSRELAQEIARLRPPRVIDLVERDRAVLQAEAERQALQNQHTEAGSAAA
                     RARDQAQAWREAHKVQAWFHDKGIGHAPKLRELEQQREEHRAEWQRLGQRIEEASLRVQ
                     HVRQQAHQRITAEQEPTLAKLAELEALQKEKARQEREAEAKRLQQQRIEREREEESMAA
                     LEHRLAELRAAQEADATQPEPSDDDPRWAAFERTFMHAAQFEHYLSLTVLAAPLAGLKA
                     RFAAYMDTAAEQGMTPEMFAQACAKGMAEQERRAMQEQAARDADPKQPQEDSPVSTPTP
                     "
                     /name="mobA"
     gene            2113..2547
                     /gene="traD"
     CDS             2113..2547
                     /gene="traD"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="BDV50523.1"
                     /translation="MTQDKWLAERLAYLRGLKAPSDQQRLLLMLADKPDRTADDGRKLA
                     ALVRAEKAAERAQKARADAARIINAERAAERKARDHELYESAGLMILAGLIDTKTGKPT
                     RDRGELLGALVSLAEAQVDDAKRAAWKAKGDALMAERARR"
                     /name="traD"
     gene            complement(2603..3493)
                     /gene="kfrA"
     CDS             complement(2603..3493)
                     /gene="kfrA"
                     /codon_start=1
                     /transl_table=11
                     /product="KfrA-like DNA binding protein"
                     /protein_id="BDV50524.1"
                     /translation="MARQGITRDQVFTAADALVGDGQQPTINAIRERLGGTGSPNTIHK
                     HLVQWREARPVAAVAAPELPQALTAAIAGEIEKAAAKARAEKEQELQQAQAEAAELGAA
                     GEALEAERDELAEQVAVLTTERDTLAGKAAQQAADLAEAQQRIEREQQAAEAARVEVAT
                     ARLKIEAQTERQTEQAAEIERLRAALAEAQQGRTAAEQQAAVLAAKLEACADRAEQIEQ
                     QAAKAAQAHDTARAAAAQEIRQAQAERDEARKVAAEAREQAARLAGQLEALQRQQDKAE
                     AAEKATKAPKGKGGE"
                     /name="kfrA"
     gene            complement(4494..5654)
                     /gene="repA"
     CDS             complement(4494..5654)
                     /gene="repA"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="BDV50525.1"
                     /translation="MAANDQEIWVSAFDVLIAQLDEALIELRRSLASVAIEHGVDSPQV
                     AGVRAQIDEATAKREKAQAERQRVMAQLAAGVSVAVTTEKPKATPKRKQPGILTQSDLK
                     LIEASAEIMSTPPDGEDMAFTHSVLCQVGLPRSKFDGREFMRQSGAAWINVQAGWLDEG
                     KGPVQQPVPYGPLPRLALAWISTQAVRTKDREIAIGSSASEFLRLLGKPTTGGVRGSFT
                     TLRKQMHALAACRLQLGFKGRTFNGQPVEQFDAWLANRETGQQALWPGLLVLSDGYFNS
                     LVENAVPLDNRALMALSDSALALDVYTWLAHRLHRIEGRGVTLHWKAIREQFAQEYKGK
                     DPDKDFKKEFVPAIKKVLAVYPQAKVKPVTGGVLLLGSPPPIAYKG"
                     /name="repA"
     gene            complement(5670..5954)
                     /gene="repF"
     CDS             complement(5670..5954)
                     /gene="repF"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="BDV50526.1"
                     /translation="MAITKKPTKPAQDATAAAADAFISGAPDAAAATPEAPRRVRKGRK
                     VQITLTIAEPLLDRVDELAGQIGQSRAAVINLAVMQMLQSGLRIEGEER"
                     /name="repF"
     gene            complement(5993..6634)
                     /gene="parA"
     CDS             complement(5993..6634)
                     /gene="parA"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein"
                     /protein_id="BDV50527.1"
                     /translation="MIITVGNTKGGVGKTTIAVNLAIARALAGRDVWLIDGDRQGTAQT
                     AISIRAEAGHTPGIACATYPDGPTLRAQVQQQAAKFDDVVIDAGGRDSTALRAALVLSD
                     VLLVPFQPRSYDVWALNDIAALVDEARSVRDGLRCFAVLNCADPGEHSTDNAEAAAAVA
                     EVPQFEYLPTPLRRRKAFSNAAGAGQCVLEIKPQDGKASAELDALISALF"
                     /name="parA"
     CDS             complement(6818..7393)
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="BDV50528.1"
                     /translation="MALIGYARVSTAEQDTALQTDALRNAGCERVFEDTASGAKADRPG
                     LADALAYLRDGDVLVVWRLDRLGRSLPHLIETVGKLEARGVGFRSLTENIDTTTPGGRL
                     IFHVFGALGQFERDLIRERTKAGLTAAAARGRKGGRKPVVTADKLQRARELVANGLNVR
                     EAAARLKVGKTALYAALQAASSGSAADS"
                     /name="recombinase family protein"
     CDS             complement(7520..7768)
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="BDV50529.1"
                     /translation="MQSSIERTVSGVRCTNDDLKPVLVALATDLPLDVRYRDHDLSGDW
                     AGYRECHIKPDLLLIYRKSDADTLRLARLGSHSELFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(8028..9041)
                     /gene="intI1"
     CDS             complement(8028..9041)
                     /gene="intI1"
                     /codon_start=1
                     /transl_table=11
                     /product="class I integron integrase"
                     /protein_id="BDV50530.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     gene            9393..10049
                     /gene="qnrVC4"
     ARG             9394..10046
                     /name="qnrVC4"
                     /gene="qnrVC4"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrVC4"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            10306..10638
                     /gene="qacL"
     ARG             10307..10635
                     /name="qacL"
                     /gene="qacL"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacL"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            10727..11281
                     /gene="aacA4"
     CDS             10727..11281
                     /gene="aacA4"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N(6)-acetyltransferase"
                     /protein_id="BDV50533.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLAD
                     VQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQSLA
                     NASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFEKQGTVTTP
                     DGPAVYMVQTRQAFERTRSDA"
                     /name="aacA4"
     gene            11496..12755
                     /gene="cmlA5"
     ARG             11497..12752
                     /name="cmlA5"
                     /gene="cmlA5"
                     /gene_name="chloramphenicol efflux MFS transporter CmlA5"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     gene            12874..13773
                     /gene="blaVEB-1"
     ARG             12875..13770
                     /name="blaVEB-1"
                     /gene="blaVEB-1"
                     /gene_name="extended-spectrum class A beta-lactamase VEB-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            13918..14451
                     /gene="aadB"
     CDS             13918..14451
                     /gene="aadB"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 2-nucleotidyltransferase"
                     /protein_id="BDV50536.1"
                     /translation="MDTTQVTLIHKILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVMEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRLACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            14510..14992
                     /gene="mobC"
     CDS             14510..14992
                     /gene="mobC"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDV50537.1"
                     /translation="MRTLKVSLMAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLL
                     VGRKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGE
                     IYRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
                     /name="mobC"
     gene            15622..16386
                     /gene="IS6100"
     CDS             15622..16386
                     /gene="IS6100"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDV50538.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6100"
     CDS             complement(16694..17041)
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV50539.1"
                     /translation="MKVWAFDENRADFVDAAPERMNADATGYYIIRRGDDVLVREHGQE
                     RPMPERLTVAGVPFDREQFEHTHPLPLRAEMKRDGQWARAQLQARDRAEIALPLELPIW
                     VTKTPLTEGKA"
                     /name="hypothetical protein"
     ARG             10728..11278
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             13919..14448
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             14520..14989
                     /name="dfrA14"
                     /gene="dfrA14"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4495..5654)
                     /name="rep_cluster_658"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(354..1924)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tggtgaagtg ccacgctatt gccaccgtat gcacacacag ccgccacagt gtcgccactg
       61 tcttaccaat atcccgccac catcgttcca ccatgtcacc accttgttac cgtcatcatt
      121 gacaccatgc cgccaccgtc tttccacact gttcccacca ttgccggtgg catcatgggg
      181 tgggagtaga aacaggggag tcctcttgtg gctgtttggg atcggcatcc ctcgccgcct
      241 gttcctgcat agcccgccgc tcctgttcgg ccatgccttt tgcgcaggcc tgagcgaaca
      301 tttccggcgt catgccttgc tccgcagcgg tatccatgta cgccgcgaaa cgtgctttga
      361 gtccggcaag cggtgcggcc agaacggtca agctgaggta gtgctcgaac tgggctgcgt
      421 gcatgaaggt gcgttcaaac gccgcccagc gaggatcatc atcgctgggc tccggctgag
      481 tggcgtcagc ctcttgcgcg gcccgcagtt ccgctagacg gtgctccagc gcggccatcg
      541 actcttcctc gcgctcgcgc tcgatgcgct gctgctgtag ccgcttcgcc tcggcctcgc
      601 gctcctggcg ggccttctcc ttctgcaagg cttccagctc ggccagcttc gccagcgtcg
      661 gctcctgctc ggcggtgatg cgctggtgcg cctgctggcg cacgtgctgc acgcgcaagc
      721 tggcctcctc gatgcgctgc ccaagtcgct gccactcggc gcggtgttcc tcgcgctgct
      781 gttccagctc gcgcagcttg ggcgcatgac caatgccctt gtcgtggaac cacgcctgca
      841 ctttgtgcgc ctcccgccat gcctgcgcct ggtcgcgggc gcgggctgcg gcgctgcccg
      901 cctcggtgtg ctggttctgt agcgcctgcc gctcggcctc ggcctgtagt acagcgcggt
      961 cgcgctctac caggtcgatc actcgcggcg ggcgcaggcg ggcaatctcc tgggccagct
     1021 cgcggctgct catgcgctcc aggcgctggc gctcctgctg cgccttctcc cgctcggccc
     1081 gctctcgggc ctcccgctgg gcctgttcgc ggcgctgccg ctcggcttcc aggtggatga
     1141 tttcggcgcc ggtatccggc accgcgcgcc gcagctcggc ggcggcctgg gccacctcgg
     1201 cgcgggcggc gcgaaactcg atcacctggg cgcgaccttc gccgcgccag gcgctcggca
     1261 actgcttgcg ctccggctcc aggccggtgc cgcgctcggc gtggctgcgc atgtcgatgc
     1321 gctgctccac cccggcccgc tccaggtgcg cattgcacat ggcctcccag cgcccgcgca
     1381 gctctttcag ctcggcggcc cgctcggcct tggtcaaggt ctggcccgcg ctcgggccgt
     1441 agcccttgcg tgcgccgccc ttctccgggg ccttggcgtt gtaccgcttg aagtactgct
     1501 ccgggtcgcg gtcgatgcca tccacctgtc gctccgaaaa catcaggtgg aagtgcggct
     1561 gctcgccacc atcggcggcg gtcggtacat ggagggccca ctggtaggcg tgccggtcgc
     1621 ctatctcctg gcgtacaaac tcgcgcacca gctcgatccg ctgcgcggcg ctcagctcgc
     1681 gcggcagggc aatttccatc tcccggtagg tcgtgccgtt cttgcgctcg taggcgtcgg
     1741 cggcctgcca gaaggcaagc gggttggact gcgcccaggc gggcatgttg cccgcctcgg
     1801 tcgcttccaa cttttcgccg cgctctaggc ggttggcgta ctggccttcg cgggcgatgt
     1861 aggcggcatg aggcccggcc ttaccggcct tgcctacctt catgctcaat cgcccgattg
     1921 ccaccgccgc cgctcctcgt gttttttgcg ccgtaggcgc tggggtgcag gggcttcccc
     1981 tgccgcgcgt ttctcggctt gccgagaaac aactaagcgc gccttccgta aaacggaagg
     2041 ataccgcagc cgtcgcgccc cggccacctt ccgcgtaaaa tgggagcctc catcacagag
     2101 ggagacgcac ccatgacaca agataagtgg ctggccgaac ggctggcata cctgcgcggc
     2161 ttgaaggctc ccagcgatca acagcgcctg ctgctgatgc tggccgacaa gcccgaccgc
     2221 accgccgacg atggccgcaa actggccgcc ctggtacgcg ccgagaaggc cgccgaacgc
     2281 gcccagaagg cccgcgccga cgccgcccgc atcatcaacg ccgagagggc cgccgagagg
     2341 aaggcccgcg accatgagct gtacgagtcg gcgggcctga tgatcctggc cgggctgatc
     2401 gataccaaga ccggcaagcc gacccgcgac cggggcgaac tgctgggcgc tctggtgtcg
     2461 ctggccgagg cccaggtcga tgacgccaag agggcagctt ggaaggccaa aggcgatgcc
     2521 ctgatggccg agagggcgcg gcgatgacgg actacggcta tgccatcgac ccccgccccg
     2581 ccgaactggg cggcggctgg catcactccc cccctttccc tttcggtgcc ttggtggcct
     2641 tctcggcggc ctcggccttg tcctgctgcc gttgcagcgc ctcaagctgc ccggcaagtc
     2701 gtgcggcctg ctcgcgggcc tctgctgcca ccttgcgggc ctcgtcacgc tcggcctgcg
     2761 cctgccggat ttcctgcgcc gctgctgcgc gggcggtgtc gtgggcctgt gcagccttgg
     2821 cggcctgctg ctcgatctgc tcggcgcggt cggcgcacgc ctccagcttg gcggccagca
     2881 cggcggcctg ctgctcggcg gcggtgcggc cctgctgtgc ctcggccagt gcggcgcgta
     2941 ggcgctctat ctctgcggcc tgctccgtct ggcgctcggt ctgcgcttcg atcttgagcc
     3001 gtgccgttgc cacctccacg cgggcggcct ctgccgcctg ctgctcgcgc tcgatgcgct
     3061 gctgcgcctc ggccaagtcg gcggcctgct gggcggcttt gcctgccagc gtgtcgcgct
     3121 ccgtggtgag gacggccacc tgctcggcca gctcgtcgcg ctcggcctcc agcgcctcgc
     3181 ctgcggcccc aagttcggcg gcctcggcct gcgcttgctg gagttcttgc tctttctcgg
     3241 cccgcgcctt cgctgcggcc ttctcgatct cgccagcaat ggcggcagtc agcgcctgcg
     3301 gcaactccgg ggccgcaacg gcagccaccg ggcgggcctc acgccattgc acaagatgct
     3361 tgtggatcgt gtttggcgac ccggtgccgc ccaaacgctc ccggatcgca ttgatggtgg
     3421 gctgttgccc gtcgcccacg agggcgtcag cggcggtgaa aacttgatcc cgagtgatgc
     3481 cttgacgtgc catgatcctt tctcctttcg tatcgtatta ctgtatttcg tattgatacg
     3541 ataccataca gctatacagt acgatacaca agcggttttc gtaacgtacc gctgcccggt
     3601 cggctgctcg tttttggtga aacaccgtgc tgacgcctcg ccttcggctc gttgtcggcc
     3661 agccgtcgaa cgaaaagcgg agctttccgc ccgccgcccg gctccacttg ggcaaagcgg
     3721 ccagaaacgg gcctacagcg cgttttccgg tcttggggtg gcctgggtta tgccagaccc
     3781 ctaaaacggc cctacgggcc gctgtggcgc ttcctgatcg cttttcgagt ggaaaggggg
     3841 gtcaagggtc tccggggcgg cccggccctt gacgcccttg gaacgaccca agcctatcgg
     3901 cagacgggag gaagaagccg aaggcgcaca tcctcccggc tagcccccac gggcaatgca
     3961 gtgctcccct tcccagcggg aaggggcggg ggggatggga gagtgtcggc cagtctcggg
     4021 gcttgttcgg agtgctggcc gttggcctgc ctttctgaaa aaccgctttt gcggcctttg
     4081 ggtgccaact gctgtttgtc caggctgagg cggtggggct gcgtgcgcgg cttgtccgcg
     4141 cttggtgaac ggctggccgc ttgcggccac attttcagcc ggagggggag ccgcccgaag
     4201 ggtgggggaa ccccgcaggg gtgccccggc ctttaggccg ctttcagcgt cgattttgcg
     4261 tggtggcttg agttatccac cgataggcgc agccaattat cggtaaatat ttataggtta
     4321 aaacattaaa ggcataaccg taccggttta gggggggggc gattcagcgt gaaaaggggg
     4381 ggcgattcag cgtggttttc ggcacaaggg ggggggcgat tcagcgtgat ggggggggcg
     4441 attcagcgtg gataacttgc ttcatccaca gggtttatcc ccaccccggc ccctcagccc
     4501 ttgtatgcga tggggggggg cgaccccagc agcagcacgc cgcccgtgac gggcttgacc
     4561 ttggcctgcg gatagacggc taacaccttc ttgatcgcgg gcacaaactc ctttttgaag
     4621 tccttgtcgg ggtctttccc cttgtactcc tgcgcgaact gttcgcggat ggccttccag
     4681 tgaagggtga cgcctcgccc ctcaatgcga tgtagccggt gggcaagcca tgtgtacacg
     4741 tccaaagcca gcgcggaatc agacaaggcc atgagggcgc ggttatcgag cgggacggcg
     4801 ttctctacca gtgagttgaa gtaaccatca gacaacacca acaggcccgg ccaaagcgcc
     4861 tgctggcccg tttccctgtt cgccagccaa gcgtcgaact gctccaccgg ctgcccgttg
     4921 aacgtgcgac ccttgaagcc tagttgcagg cggcaggctg caagggcgtg catctgcttc
     4981 cgcagagtgg tgaacgatcc gcgcaccccg ccagtggtgg gcttacccaa cagtcgcaga
     5041 aactcgcttg cgctgctgcc gatggcaatt tcgcggtctt tggttcgcac ggcctgcgtg
     5101 gagatccacg ccagcgccaa gcgtggcaaa gggccatagg ggacgggctg ctgcacaggc
     5161 cccttgccct catccagcca ccccgcctgc acgttgatcc aagctgcgcc ggattggcgc
     5221 atgaactcgc ggccatcgaa tttggagcgc ggcaggccga cttggcacag caccgaatgg
     5281 gtaaaggcca tgtcctcacc atcgggcggc gtgctcatga tctccgccga tgcctcaatc
     5341 agcttgaggt cggattgtgt caggatgccc ggctgtttgc gcttgggtgt cgccttgggc
     5401 ttttcggtag tgacggcaac agacacaccg gcggcaagct gggccatgac gcgctgccgc
     5461 tcggcctgcg ccttctcgcg cttcgcggtg gcttcgtcga tctgcgcccg gactcccgca
     5521 acctgtgggc tatcgacacc gtgctctatg gctacgctgg caagcgaccg gcgcaactca
     5581 atgagagctt catcaagctg ggcgatgaga acatcgaacg ctgataccca aatctcttga
     5641 tcgttggcgg ccatagcccc ctcccctctt tatcgttcct cgccctcgat gcgtagcccg
     5701 ctctggagca tctgcatcac tgccagattg atgacagccg cccggctctg cccaatctgg
     5761 ccagccagtt catcgacacg atccagcagc ggctcggcaa tggtcagcgt gatttgcacc
     5821 ttgcgaccct tgcgcacccg gcgcggagct tccggggtgg ctgcggcagc atccggcgca
     5881 ccggaaatga aggcatccgc cgccgctgcg gtagcgtctt gcgcgggctt cgtcggtttc
     5941 tttgtaatcg ccattgaatc tatctccaca cttaaagcat attgatttga tattaaaaca
     6001 atgcgctgat aagtgcatca agttccgcgc ttgctttgcc gtcttgcggc ttgatttcaa
     6061 gcacacactg ccccgcccct gcggcgttgg aaaacgcctt gcggcggcgt agcggggtcg
     6121 gcaggtactc gaactggggc acctcggcca cggcggcggc ggcctcggcg ttgtcggtgg
     6181 agtgctcgcc cggatcggcg cagttgagga cagcaaagca gcgcaaaccg tcgcgcacac
     6241 tgcgggcctc atcgaccagc gccgcaatgt cgttgagcgc ccacacgtca tagctgcgcg
     6301 gctggaacgg caccagcagc acgtcagaca gcacgagggc tgcgcgtagc gcggtcgagt
     6361 cgcgcccgcc tgcgtcgatc accacgtcat cgaacttggc cgcttgctgc tgcacctgtg
     6421 cccgcagggt cgggccgtcc gggtaggtcg cgcaggcaat gcccggcgtg tgcccggcct
     6481 cggcacgaat gctgattgct gtctgcgcgg tgccctgacg atcaccgtcg atcagccaca
     6541 catcacgccc ggccaatgcg cgggcgattg ccagattcac ggcaatggtg gttttcccca
     6601 cgccgccctt tgtgtttcca acggtgataa tcatcgcctt gtcacctcaa cttaatatta
     6661 aaaacagttg ctattgtagt gaaaaaagga tgcttgtcaa tgcttgccgc aggcataaaa
     6721 aaccccgcac gcagcggggt tcggtggcag ggcaggggag ggcttaggcg atctggtgga
     6781 tctcgttgtc gttttcagaa ggcgactgca cgaaatatca ggagtcggct gcactgccag
     6841 aactggccgc ctgcaaggca gcgtagagag cggtttttcc taccttgagg cgtgcggcgg
     6901 cctcgcggac attcaagccg ttggcgacaa gctcccgcgc tcgttgcaac ttgtcggcag
     6961 tgacaaccgg cttgcgtccg cctttgcgcc cgcgtgcggc ggcagcggtc aacccggcct
     7021 tggtgcgctc gcggatcagg tcgcgctcga actggcccag cgcaccgaac acatggaaga
     7081 tgagccgtcc gcccggcgtg gtggtgtcga tgttttcagt caacgagcgg aagccgacgc
     7141 cccgtgcttc cagcttgccg accgtctcaa tcaggtgcgg cagggagcgg ccaagccgat
     7201 ccagccgcca gacgaccagc acatcgccat cgcgcagata agccagcgca tcggccaagc
     7261 cgggccggtc tgccttggcc ccggatgccg tgtcctcgaa aacccgttcg cagcctgcat
     7321 tgcgcagcgc atccgtctgc aaggcggtgt cctgttccgc cgtcgatacc cgcgcatagc
     7381 cgatgagtgc catttccgcc catttgtccg ttataccgtc cgcctatctt attgtccgtc
     7441 atcccgttgc gcaagatgtt ttcggacagg cgtccggttt ggccgcctaa tgatcgtttg
     7501 gcggacaagg cgactggcat cagccgaaca gctcgctatg ggagccaagc cgcgccagtc
     7561 gcagggtgtc ggcgtcggac ttgcggtaga tcagcagcag gtcgggcttg atgtggcatt
     7621 cgcggtagcc cgcccaatcg ccggaaagat cgtggtcgcg gtatcgcaca tccaggggca
     7681 gatcggtcgc cagcgcgacc agaaccggct tgaggtcatc attcgtgcag cggactcctg
     7741 atactgttcg ttcaatagac gactgcacaa cagccttcaa accgcgttgt ctggtgcagt
     7801 cgtcttctga aaatgacaca aaaactgtcg ttttcagaag acggctgcac tgaacgtcag
     7861 aagccgactg cactatagca gcggaggggt tggatccatc aggcaacgac gggctgctgc
     7921 cggccatcag cggacgcagg gaggactttc cgcaaccggc cgttcgatgc ggcaccgatg
     7981 gccttcgcgc aggggtagtg aatccgccag gattgacttg cgctgcccta cctctcacta
     8041 gtgaggggcg gcagcgcatc aagcggtgag cgcactccgg caccgccaac tttcagcaca
     8101 tgcgtgtaaa tcatcgtcgt agagacgtcg gaatggccga gcagatcctg cacggttcga
     8161 atgtcgtaac cgctgcggag caaggccgtc gcgaacgagt ggcggagggt gtgcggtgtg
     8221 gcgggcttcg tgatgcctgc ttgttctacg gcacgtttga aggcgcgctg aaaggtctgg
     8281 tcatacatgt gatggcgacg cacgacaccg ctccgtggat cggtcgaatg cgtgtgctgc
     8341 gcaaaaaccc agaaccacgg ccaggaatgc ccggcgcgcg gatacttccg ctcaagggcg
     8401 tcgggaagcg caacgccgct gcggccctcg gcctggtcct tcagccacca tgcccgtgca
     8461 cgcgacagct gctcgcgcag gctgggtgcc aagctctcgg gtaacatcaa ggcccgatcc
     8521 ttggagccct tgccctcccg cacgatgatc gtgccgtgat cgaaatccag atccttgacc
     8581 cgcagttgca aaccctcact gatccgcatg cccgttccat acagaagctg ggcgaacaaa
     8641 cgatgctcgc cttccagaaa accgaggatg cgaaccactt catccggggt cagcaccacc
     8701 ggcaagcgcc gcgacggccg aggtcttccg atctcctgaa gccagggcag atccgtgcac
     8761 agcaccttgc cgtagaagaa cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa
     8821 accttgcgct cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt
     8881 gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt
     8941 tcggttcgta agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg
     9001 accgaacgca gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt
     9061 tttgtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     9121 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     9181 tgtgtttttc ttgcaagcgc catgattaat caaaactttt ttctagccat gcggcatggg
     9241 tgaattaaaa aacagtgcct tagagcgtca atttggtacc ttatttttgc gattttaagt
     9301 ggttgtaagt tgctgttttt aaggtgttta tttttcttga gaactggttg gtaaatgtta
     9361 gtcttcgact caaatagaaa gagggctaga aaatggataa aacagaccag ttatatgtac
     9421 aagcagactt ttcacatcaa gacatgagtg gtcagtattt taaaaattgc aaatttttct
     9481 gctgttcctt taaacgagcg aacctccgcg atacacaatt tgtagattgt tctttcattg
     9541 aacgaggtga attagagggg tgtgattttt cttactcgga tcttagagat gcatctttta
     9601 aaaactgcag tctttcaatg tcgtatttca aaggtgcaaa ttgttttggt atcgagttca
     9661 gagaatgcga tttaaagggt gccaattttg ctcaagctag cttcatgaat caggtatcga
     9721 acagaatgta tttttgttca gcttatataa caggttgtaa tctttcatac gccaactttg
     9781 aaaggcagtg tatcgaaaag tgtgatttgt ttgagaatag atggattggc gcaaatctga
     9841 gtggtgcatc atttaaagag tctgatttaa gtcggggagt attttctgaa gggtgttgga
     9901 gccagtgtag gttgcaaggt tgtgatttga gccactcgga gttgtatggt ttagaccctc
     9961 ggaaagttga ccttacaggt gtaaaaatct gttcgtggca gcaagaacaa cttttagagc
    10021 aattaggttt aatagtagtt cctgactaaa tttgatggtt tgaacacaca cataacaaac
    10081 gcctcaagag ggactgtcaa cgcgtggcgt ttccagtccc aatgagctgt ggtggttacg
    10141 gctgttgtgt ttgggtttag tgtcatgcgt tgccagcccc ttaggcgggc gttagatgcc
    10201 agatttggcg ttgcgtatgc tcacagaaaa tcgacagccg caagactgtt tttggcaact
    10261 catagccacc actatttatc cttcataccg tagaggagat tgcacgtgaa gaactggctc
    10321 tttctggcta ttgcaatatt tggtgaggtc gtcgcaactt ccgcactgaa gtccagccat
    10381 ggattcacca agttagttcc ttctgttgta gttgtggctg gctacgggct tgcgttctat
    10441 ttcctctctc tcgcaatcaa gtccatcccg gtcggcattg cttatgctgt ttgggctggc
    10501 ctcggcatcg tacttgtggc agctatcgct tggatcttcc atggccagaa actagacttg
    10561 tgggcgttcg ttggcatggg acttatcgtt agtggcgtcg ccgttctaaa tctgctatcc
    10621 aaggtcagcg cacattgatc gggctggcat ctaacaattc attcaagccg acgccgcttc
    10681 gcggcgcggc ttaattcagg tgttaggcat cacaaagtac agcatcgtga ccaacagcaa
    10741 cgattccgtc acactgcgcc tcatgactga gcatgacctt gcgatgctct atgagtggct
    10801 aaatcgatct catatcgtcg agtggtgggg cggagaagaa gcacgcccga cacttgctga
    10861 cgtacaggaa cagtacttgc caagcgtttt agcgcaagag tccgtcactc catacattgc
    10921 aatgctgaat ggagagccga ttgggtatgc ccagtcgtac gttgctcttg gaagcgggga
    10981 cggatggtgg gaagaagaaa ccgatccagg agtacgcgga atagaccagt cactggcgaa
    11041 tgcatcacaa ctgggcaaag gcttgggaac caagctggtt cgagctctgg ttgagttgct
    11101 gttcaatgat cccgaggtca ccaagatcca aacggacccg tcgccgagca acttgcgagc
    11161 gatccgatgc tacgagaaag cggggtttga gaagcaaggt accgtaacca ccccagatgg
    11221 tccagccgtg tacatggttc aaacacgcca ggcattcgag cgaacacgca gtgatgccta
    11281 agtcaaacgt tgggcgcaca ataaggctcc tcgcagagtt gcttgaaagt tgttacgatt
    11341 caaattcaat catgagatag tcagcagatg agcacttcca agaacgcaga caagtaagcc
    11401 gcagcaacct tcatttttcg gttgttgcgg cgttctcatg aatccttttg ctctacggga
    11461 gcgccgccaa atcctttgtt caaggagatg gtttcgtgcg ctcaaaaaac tttagttggc
    11521 ggtactccct tgccgccacg gtgttgttgt tatcaccgtt cgatttattg gcatcactcg
    11581 gcatggacat gtacttgcca gcagtgccgt ttatgccaaa cgcgcttggt acgacagcga
    11641 gcacaattca gcttacgctg acaacgtact tggtcatgat tggtgccggt cagctcttgt
    11701 ttggaccgct atcggaccga ctggggcgcc gccccgttct actgggaggt ggcctcgcct
    11761 acgttgtggc gtcaatgggc ctcgctctta cgtcatcggc tgaagtcttt ctggggcttc
    11821 ggattcttca ggcttgtggt gcctcggcgt gccttgtttc cacatttgca acagtacgtg
    11881 acatttacgc aggtcgcgag gaaagtaatg tcatttacgg catactcgga tccatgctgg
    11941 ccatggtccc ggcggtaggc ccattgctcg gagcgctcgt cgacatgtgg cttgggtggc
    12001 gggctatctt tgcgtttcta ggtttgggca tgatcgctgc atctgcagca gcgtggcgat
    12061 tctggcctga aacccgggtg caacgagttg cgggcttgca atggtcgcag ctgctactcc
    12121 ccgttaagtg cctgaacttc tggttgtaca cgttgtgtta cgccgctgga atgggtagct
    12181 tcttcgtctt tttctccatt gcgcccggac taatgatggg caggcaaggt gtgtctcagc
    12241 ttggcttcag cctgctgttc gccacagtgg caattgccat ggtgtttacg gctcgtttta
    12301 tggggcgtgt gatacccaag tggggcagcc caagtgtctt gcgaatggga atgggatgcc
    12361 tgatagctgg agcagtattg cttgccatca ccgaaatatg ggcttcgcag tccgtgttag
    12421 gctttattgc tccaatgtgg ctagtgggta ttggtgtcgc cacagcggta tctgtgtcgc
    12481 ccaatggcgc tcttcgagga ttcgaccatg ttgctggaac ggtcacggca gtctacttct
    12541 gcttgggcgg tgtactgcta ggaagcatcg gaacgttgat catttcgctg ttgccgcgca
    12601 acacggcttg gccggttgtc gtgtactgtt tgacccttgc aacagtcgtg ctcggtctgt
    12661 cttgtgtttc ccgagtgaag ggctctcgcg gccaggggga gcatgatgtg gtcgcgctac
    12721 aaagtgcgga aagtacatca aatcccaatc gttgagagaa tgtggcaagc tatcgcccaa
    12781 caaatcgctg cagccgaccc aaaaccgcta cgcggtttcg gtcggctgag ctcaggcgtt
    12841 agcggtaatt taaccagata ggagtacaga catatgaaaa tcgtaaaaag gatattatta
    12901 gtattgttaa gtttattttt tacaattgtg tattcaaatg ctcaaactga caacttaact
    12961 ttgaaaattg agaatgtttt aaaggcaaaa aatgccagaa taggagtagc aatattcaac
    13021 agcaatgaga aggatacttt gaagattaat aacgacttcc atttcccgat gcaaagcgtt
    13081 atgaaatttc cgattgcttt agccgttttg tctgagatag ataaagggaa tctttctttt
    13141 gaacaaaaaa tagagattac ccctcaagac cttttgccta aaacgtggag tccgattaaa
    13201 gaggaattcc ctaatggaac aactttgacg attgaacaaa tactaaatta tacagtatca
    13261 gagagcgaca atattggttg tgatattttg ctaaaattaa tcggaggaac tgattctgtt
    13321 caaaaattct tgaatgctaa tcatttcact gatatttcaa tcaaagcaaa cgaagaacaa
    13381 atgcacaagg attggaatac ccaatatcaa aattgggcaa ccccaacagc gatgaacaaa
    13441 ctgttaatag atacttataa taataagaac caattacttt ctaaaaaaag ttatgatttt
    13501 atttggaaaa ttatgagaga aacaacaaca ggaagtaacc gattaaaagg acaattacca
    13561 aagaatacaa ttgttgctca taaaacaggg acttccggaa taaataatgg aattgcagca
    13621 gccactaatg atgttggggt aattacttta ccgaatggac aattaatttt tataagcgta
    13681 tttgttgcag agtccaaaga aacttcggaa attaatgaaa agattatttc agacattgca
    13741 aaaataacgt ggaattacta tttgaataaa taaaaaacta ccgctaacac tggctcatag
    13801 gcaatggcgg gttgaagtgc aatttgcaaa gtcggtagcc cgcccgagcg ttttctcggt
    13861 ttgacaggaa aggctcacgc aaaccgccac tgcccatagc ccaaaccgtt aggccgcatg
    13921 gacacaacgc aggtcacatt gatacacaaa attctagctg cggcagatga gcgaaatctg
    13981 ccgctctgga tcggtggggg ctgggcgatc gatgcacggc tagggcgtgt aacacgcaag
    14041 cacgatgata ttgatctgac gtttcccggc gagaggcgcg gcgagctcga ggcaatagtt
    14101 gaaatgctcg gcgggcgcgt catggaggag ttggactatg gattcttagc ggagatcggg
    14161 gatgagttac ttgactgcga acctgcttgg tgggcagacg aagcgtatga aatcgcggag
    14221 gctccgcagg gctcgtgccc agaggcggct gagggcgtca tcgccgggcg gccagtccgt
    14281 tgtaacagct gggaggcgat catctgggat tacttttact atgccgatga agtaccacca
    14341 gtggactggc ctacaaagca catagagtcc tacaggctcg catgcacctc actcggggcg
    14401 gaaaaggttg aggtcttgcg tgccgctttc aggtcgcgat atgcggccta acaattcgtc
    14461 caagccgacg ccgcttcgcg gcgcggctta actcaggtgt taacccagga tgagaacctt
    14521 gaaagtatca ttgatggctg cgaaagcgaa aaacggcgtg attggttgcg gtccagacat
    14581 accctggtcc gcgaaagggg agcagctact ttttaaagca ttgacctaca atcagtggct
    14641 tctggtgggt cgcaagacgt ttgaatctat gggcgcactc cccaatagga aatacgcggt
    14701 cgttacccgc tcaggttgga catcaaatga tgacaatgta gttgtatttc agtcaatcga
    14761 agaggccatg gacaggctag ctgaattcac cggtcacgtt atagtgtctg gtggcggaga
    14821 aatttaccga gaaacattac ccatggcctc tacgctccac ttatcgacga tcgacatcga
    14881 gccagagggg gatgttttct tcccgagtat tccaaatacc ttcgaagttg tttttgagca
    14941 acactttact tcaaacatta actattgcta tcaaatttgg aaaaagggtt aacaaagcta
    15001 tgcaatcgac ggcaaaaagc ttcgttcgct tcgcgcacta cgccttttcc gcgattgata
    15061 gcgacgttat gtgaatattg aaatgagcat tccaaagaaa ggaagtagaa aaattatcgt
    15121 gggcgaaaat gagtttttgt ggctcattag gtcaaaactc acatattcgc aggactgttt
    15181 aggtacagaa atgactgcgg tcgttgaacc ggatgccggt cctcactgat gaagaagtga
    15241 acaccgtacg ggaatcgtgc cggcagcttg gagctatcgg ccggaacctc aaccaggtgg
    15301 ccagggcctt gaacatcgag ttcagggaaa gtgacaagct caagcaagag gccatcgaaa
    15361 aactggccga acggatcgac cagcatttgg accatgtgtc tgagctgttc gataagacct
    15421 ggagccggtg gcacgattgt cattttcaga agacgactgc accagttgat tgggcgtaat
    15481 ggctgttgtg cagccagctc ctgacagttc aatatcagaa gtgatctgca ccaatctcga
    15541 ctatgctcaa tactcgtgtg ggctctgttg caaaaatcgt gaagcttgag catgcttggc
    15601 ggagattgga cggacggaac gatgacggat ttcaagtggc gccatttcca gggtgatgtg
    15661 atcctgtggg cggtgcgctg gtattgtcgc tatccgatca gctatcgcga ccttgaggaa
    15721 atgctggcgg aacgcggcat ttcggtcgac catacgacga tctatcgctg ggtccagtgc
    15781 tacgccccgg agatggagaa gcggctgcgc tggttctggc ggcgtggctt tgatccgagc
    15841 tggcgcctgg atgaaaccta cgtcaaggtg cggggcaagt ggacctacct gtaccgggca
    15901 gtcgacaagc ggggcgacac gatcgatttc tacctgtcgc cgacccgcag cgccaaggca
    15961 gcgaagcggt tcctgggcaa ggccctgcga ggcctgaagc actgggaaaa gcctgccacg
    16021 ctcaataccg acaaagcgcc gagctatggt gcagcgatca ccgaattgaa gcgcgaagga
    16081 aagctggacc gggagacggc ccaccggcag gtgaagtatc tcaataacgt gatcgaggcc
    16141 gatcacggaa agctcaagat actgatcaag ccggtgcgcg gtttcaaatc gatccccacg
    16201 gcctatgcca cgatcaaggg attcgaagtc atgcgagccc tgcgcaaagg acaggctcgc
    16261 ccctggtgcc tgcagcccgg catcaggggc gaggtgcgcc ttgtggagag agcttttggc
    16321 attgggccct cggcgctgac ggaggccatg ggcatgctca accaccattt cgcagcagcc
    16381 gcctgatcgg cgcagagcga cagcctacct ctgactgccg ccaatctttg caacagagcc
    16441 tccgtcgcca tgctcacctc gctttggtgc acacgagtat tgagcatagt cgagattggt
    16501 gcagatcact tctgatattg aactgtcagg agctggctgc acaacagcca ttacgcccaa
    16561 tcaactggtg cagtcgtctt ctgaaaatga caaaaacgcc ccttgcggca actgtccaac
    16621 aaaccaccgt tttctgaaca gtcggcaggg ctacgatgaa acacaaacaa atgcccgcag
    16681 acgggcgaca ggatcacgcc ttgccctcgg tcagcggcgt tttcgtcacc caaatgggca
    16741 attccagcgg cagggcgatt tcggcccggt cgcgggcctg caattgcgcc cgcgcccatt
    16801 ggccgtctcg tttcatctcc gctcgcagag gcagggggtg ggtgtgctcg aactgctccc
    16861 ggtcaaatgg caccccggct acggtcagcc gctcaggcat gggccgttcc tggccgtgct
    16921 cgcggactaa cacgtcatcg ccgcgccgga tgatgtagta cccggtcgca tcagcgttca
    16981 tccgctccgg cgcagcatct acgaaatcgg cccggttctc atcgaatgcc cagactttca
    17041 tgcgcaaccc tcccgaatcg acatcagatg gtggcaggaa tgtggctgca ttg
//
