LOCUS       LC744510               10494 bp    DNA     circular BCT 24-DEC-2022
DEFINITION  Escherichia coli MyNCGM645 plasmid pMyNCGM645 DNA, complete
            sequence.
ACCESSION   LC744510
VERSION     LC744510.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Complete plasmid sequence of multidrug-resistant Escherichia coli
            co-harboring blaNDM-5 and rmtB in Myanmar
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2022) Contact:Tomomi Hishinuma Juntendo University
            School of Medicine, Department of Microbiology; 2-1-1 Hongo,
            Bunkyo-ku, Tokyo 113-8421, Japan
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.7
            Sequencing Technology :: MinION; Illumina MiSeq
            ##Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MyNCGM645"
                     /db_xref="taxon:562"
                     /plasmid="pMyNCGM645"
                     /geo_loc_name="Myanmar"
                     /collection_date="2017"
     CDS             911..2452
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001120888.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="BDV21842.1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASLE
                     AQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGARRM
                     AESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIKKAG
                     YTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYDELNT
                     LAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGPQQGRK
                     VFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPAVSEKRL
                     ALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVFAPNSKHR
                     VQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVCEHCGGHVK
                     VIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQSRLFD"
                     /name="IS91 family transposase"
     CDS             2765..3796
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV21843.1"
                     /translation="MTASTIRLRRWLAGLALLLALPATSAVAQDFELPPVDEVFVLSAQ
                     ATAPDRIEVRWRIADGYYLYRHRTSVKADAAFTGATMALPKGKAYRDEFFGDVETYRKE
                     LLGTLTGTPAAGASATTLTVKYQGCADAGVCYPPQTRTLKVALPGEAGAGGFGCKARGL
                     HDYVVKNGSADHPNAEVKFALGDVVNTMIGCTNGETIMLCHDTSLPRPYSLGFRVQGTE
                     GLWMDVNKSIYLEGKSPQPHRWEPAEGWFAKYDHPLWKRYADLAAGAGHGGMDWFVIHA
                     FVEALKAKAPMPIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRKPIFALN
                     DAY"
                     /name="hypothetical protein"
     gene            complement(3807..4445)
                     /gene="trpF"
     CDS             complement(3807..4445)
                     /gene="trpF"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:E8TBD8"
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="BDV21844.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVAG
                     ALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKPVW
                     KALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALPWGL
                     AGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
                     /name="trpF"
     CDS             complement(4450..4815)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010920626.1"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="BDV21845.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQL
                     EFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLRIG
                     YLIDPDCTLVRLIQNPD"
                     /name="bleomycin resistance protein"
     gene            complement(4819..5631)
                     /gene="bla"
     CDS             complement(4819..5631)
                     /gene="bla"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:Q6HFY5"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="BDV21846.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQR
                     FGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLLVDTAWTDDQTAQILNW
                     IKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHSLTF
                     AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG
                     DADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
                     /name="bla"
     CDS             complement(5732..5926)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV21847.1"
                     /translation="MNGLIRQYLPKGIDLNQADQHYLNQVAMSLNTRPRKALDWLTPLE
                     KFAQLVDYHMAFETVAPHV"
                     /name="hypothetical protein"
     CDS             6172..6894
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001313567.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDV21848.1"
                     /translation="MLMELHMNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERG
                     VNVDHSTIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR
                     GRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCP
                     SDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAF
                     YYGDPLGEMRLVSRVFEM"
                     /name="IS6 family transposase"
     CDS             complement(6928..7815)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000845048.1"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="BDV21849.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRRALLQS"
                     /name="integron integrase"
     CDS             7960..8457
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3MV56"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="BDV21850.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGKV
                     VVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVAGG
                     AEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVYARR
                     NG"
                     /name="dihydrofolate reductase"
     CDS             8865..9656
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3EQ55"
                     /codon_start=1
                     /transl_table=11
                     /product="streptomycin 3''-adenylyltransferase"
                     /protein_id="BDV21851.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKPY
                     SDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQDLF
                     EALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQP
                     VLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
                     /name="streptomycin 3''-adenylyltransferase"
     gene            9820..10167
                     /gene="ebr"
     CDS             9820..10167
                     /gene="ebr"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000679427.1"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="BDV21852.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="ebr"
     ARG             9821..10164
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             8866..9653
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7961..8454
                     /name="dfrA12"
                     /gene="dfrA12"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4823..5631)
                     /name="blaNDM-5"
                     /gene="blaNDM-5"
                     /gene_name="subclass B1 metallo-beta-lactamase NDM-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4454..4815)
                     /name="ble"
                     /gene="ble"
                     /gene_name="bleomycin binding protein Ble-MBL"
                     /tool="amrfinderplus"
                     /drug_class="BLEOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             8878..9656
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             10162..10493
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="39.78"
     ARG             4..506
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="59.86"
     ARG             complement(4451..4815)
                     /name="BRP(MBL)"
                     /gene="BRP(MBL)"
                     /gene_name="Bleomycin resistant protein"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 atcccgatat tgctgaggcg gactgcaggc tggtggttat gcactcagcg cagcgggatg
       61 gcatcgccac ccgcaccggt caccttcgac ccgaagacgc gctcgacgag attgtgcggt
      121 tcttcgaggc gcgggtttcc gccttgcgac ggagcggggt cgctgccgac cggctcatcc
      181 tcgatccggg gatgggattt ttcttgagcc ccgcaccgga aacatcgctg cacgtgctgt
      241 cgaaccttca aaagctgaag tcggcgttgg ggcttccgct attggtctcg gtgtcgcgga
      301 aatccttctt gggcgccacc gttggccttc ctgtaaagga tctgggtcca gcgagccttg
      361 cggcggaact tcacgcgatc ggcaatggcg ctgactacgt ccgcacccac gcgcctggag
      421 atctgcgaag cgcaatcacc ttctcggaaa ccctcgcgaa atttcgcagt cgcgacgcca
      481 gagaccgagg gttagatcat gcctagcatt caccttccgg ccgcccgcta aatatctcct
      541 tttgggttgt taataaaaca tccaataagt tgactgtgcg tgaaaaagaa agttttgtgt
      601 gatggcgttg aagatcgcac cgttaagctc ttatgtggga tggtgcagag ctcgacgact
      661 accgataaaa cgcaaccgcc gcaaacagac aagaaaaagc cccaactgat aacagttggg
      721 gcttcagtat tgtgattggt ggagcaatag caccctgaac ccaaaacctt ctcgctcaac
      781 cggtagtggc tgataacaac tcgtgagggc tattgcgggt taagcattta gcgatgtcta
      841 gggccagact ggacgtctga acgcaagccg ctgatactgt acataaccac agtatcagcg
      901 gaggataccc atgtcgctgg caaggaacgc cacggcgagt caatcgccca ctcaaacaaa
      961 cggttacgaa cgccaccaac ccgaccagac gctgctctac cagctggttg agcagcacta
     1021 cccagccttc aaagcctcac tcgaagccca aggtcaacac ctgcctcgct acatccaaca
     1081 agaattcaac gacctcctcc aatgtggccg tctggagtat ggtttcatgc gggttcgctg
     1141 cgaggattgt catcacgagc gtctggtcgc cttcagctgt aaacgacgcg gcttttgccc
     1201 tagctgcggt gcccgccgga tggccgagag tgcggcgctg ctgatagacg aagtcttccc
     1261 caaggagccc attcgccagt gggtgctcag ctttcctttc cagctacgct ttttgctggc
     1321 tcgccatccc cagctgatgg gccaggtctt gagtatcgtc tatcgtacac tctcaactca
     1381 tctgatcaaa aaagccggtt acaccaaagc ctctgcacaa actggctcag tgactcttat
     1441 ccaacgcttt ggctccgcgc taaatctcaa tgtccactac cacatgctgt ttctcgatgg
     1501 tgtctatgcc gaagatgact atggcaagca acgcttccat cgtgtcaagg cacccactta
     1561 cgatgagctg aatacgctcg ctcacaccct cagccatcgc atcgctcgct gcatggaaaa
     1621 gcgtgggatt ttggagcgtg atgccgagaa tacgtggttg acactggaag agggcgaaga
     1681 cgatacgctg actcaattac atggtgcttc ggttacgtat cgcattgccg tcggccccca
     1741 gcaagggcgc aaagtcttca ccctgcaaac cttgccaggg cgtgaggata aagccgactc
     1801 aagcagtcga gtagccaacc atgctggttt ctcgctacac gccggtgtga tggccgaagc
     1861 gcatcagcgg gataagcttg agcgcttgtg tcgctacatt agtcggccag cggtttcaga
     1921 aaaacgtctg gcattaaccg ccaatgggca ggtgcgttac gagctcaaaa ctccgtaccg
     1981 caatggcacc acccatgtga tcttcgagcc gctggacttc atcgccaaac tcgctgcgtt
     2041 ggtacctaag ccgcgagtca acctcacacg cttccacggc gtctttgcac cgaacagcaa
     2101 acaccgagtt caagtaacac ccgccaagcg gggcaagaag cccgacaaat cggaaggtct
     2161 cgatactaac tggcgtgaca agagtcctgc agagcgccac cgcgccatga cctggatgca
     2221 acgcctcaag cgagtcttca atattgatat tgaagtctgc gaacactgcg gcggtcacgt
     2281 caaagtgatt gccagcatcg aagatccgaa ggtcattgag cagattctca agcatctgaa
     2341 acagaaaaca gccaaggcga atgccgccaa gcagcgtgag ctgccaccag aacgagcgcc
     2401 gccactgact cccagcctgt tcgatccatc acagagtcgt ctctttgact gacgacccca
     2461 aatccaacac tgctcaacac tgccaacttt taaacggggc ggtggggcag tttgtatctc
     2521 tcgagctatc aggctagaga ttttaccgcc aaatcgaacc ttattagagc ggtttaggct
     2581 ggaccggcag ttaaaattgg ggcttgagcg gtaaacgagt gagggaattt caggtaagat
     2641 acttcggatg aggagcaaaa aggtggttta tacttcctat acccgctcgc ggtcgaagcc
     2701 gcgtccggcc tgcccgaata cctgcaatgg ctggccgccg agagccgacc ggtaaactga
     2761 gccaatgacc gcatccacga tccgcctgcg ccgctggctg gccgggctcg ccctgctgct
     2821 tgcgctgccc gcgacctcgg ccgtcgccca ggacttcgaa ctgccgccgg tcgacgaggt
     2881 cttcgtcctg tccgcgcagg ccaccgcgcc ggaccgcatc gaggtgcgct ggcggatcgc
     2941 cgacggctat tacctgtacc ggcaccgcac ctcggtcaag gccgatgccg ccttcaccgg
     3001 cgcgaccatg gcgctgccga agggcaaggc ctaccgcgac gaattcttcg gcgacgtcga
     3061 aacctaccgc aaggaattgc tcggcaccct caccggcacg cccgcggccg gcgcgagcgc
     3121 gaccaccctg accgtgaagt accagggctg cgccgatgcc ggcgtgtgct acccgccgca
     3181 gacccgcacc ctgaaggtcg cgttgccggg cgaagcgggc gctggcggct tcggctgtaa
     3241 ggcgcgcggg ctgcatgatt atgtcgtcaa gaacggctct gccgatcatc ccaacgccga
     3301 ggtgaaattc gcactgggtg atgtggtcaa caccatgatc ggctgcacta atggtgaaac
     3361 gatcatgctg tgccacgaca cctcgctgcc gcgcccctat tctctcggct ttcgggtgca
     3421 aggcaccgag gggctgtgga tggacgtcaa caagtcgatc tatctggagg gcaagagccc
     3481 acagccgcac cgctgggagc ctgccgaggg ctggtttgcg aaatacgatc acccgctatg
     3541 gaaacgctac gccgatctgg cggcaggggc cgggcatggc gggatggact ggttcgtgat
     3601 ccacgctttt gtcgaggcgc tgaaggccaa ggccccgatg ccaatcgaca tttacgacgc
     3661 gctggcctgg agcgcgatca cgcctctgtc ggaacaatcg attgctgagg gcaatcgcac
     3721 gttagatttt cccgacttca cccgagggca gtggcgcacc cgcaagccga tctttgcgct
     3781 gaacgacgcc tattgatcga cgcgatttag gccaagcgca ccgcaaaggc gaaattggta
     3841 atcttgtcgg tatccttgac gcccggcgcg ctttcgacgc cgctggaggt atcgaccagc
     3901 ggcgctccgg tgcgcgcaat cgcctcggca acattcgtcg gatttagccc gcctgccagc
     3961 ccccacggca aggcaccgcg atatccggcc agcagcgacc agtcgaacgc caaccccatg
     4021 ccgccgggca gcgcgccttt gggggtcttg gcgtcgaaca agatcaagtc cgccgccccg
     4081 gcataggctg cggcgcgtgc gacatcgctg gcgctggcga cgggcagcgc cttccacacc
     4141 ggcttgccaa accgcgcgcg caactgggcc acgcgttcgg gcgattccga accgtgcagc
     4201 tgcagcgcgt tcagcttggc tgccaccagt gcgtcggcga tgacagcatc atccgcatcg
     4261 acgaacaaac cgaccatggc gatctggcca gctgcgcgcg atgtcaaagc gcccgcgaca
     4321 ttcgacgtaa ccgcacgggg cgacgctgga tagaacacca acccggcata gtccgcccgc
     4381 gccgcgatgg tcgcatcgag cgcctcgggt gtgctgatcc cgcaaatctt gattttcgcg
     4441 ggcatgcggt cagtcggggt tctggatcag ccgcaccagc gtgcagtcgg gatcgatcag
     4501 gtagccgatc ctcaggccgc tcgcctccag ttgcggagct ttgaagcgcg gccagccggt
     4561 gcttttttcc tcggctcccg ccgcgttcac caatgccacc atggcatcga gatcatccaa
     4621 ccgcaggcaa cagccgaacg agctcgtagc tgggtcgagg tcaggatagg ggaagaattc
     4681 gagctgcaaa ccgccgcgct gcaggatcat ccagccgcga tccttccaac tcgtcgcaaa
     4741 gcccagcttc gcataaaacg cctctgtcac atcgaaatcg cgcgatggca gattgggggt
     4801 gacgtggtca gccatggctc agcgcagctt gtcggccatg cgggccgtat gagtgattgc
     4861 ggcgcggcta tcgggggcgg aatggctcat cacgatcatg ctggccttgg ggaacgccgc
     4921 accaaacgcg cgcgctgacg cggcgtagtg ctcagtgtcg gcatcaccga gattgccgag
     4981 cgacttggcc ttgctgtcct tgatcaggca gccaccaaaa gcgatgtcgg tgccgtcgat
     5041 cccaacggtg atattgtcac tggtgtggcc ggggccgggg taaaatacct tgagcgggcc
     5101 aaagttgggc gcggttgctg gttcgaccca gccattggcg gcgaaagtca ggctgtgttg
     5161 cgccgcaacc agcccctctt gcggggcaag ctggttcgac aacgcattgg cataagtcgc
     5221 aatccccgcc gcatgcagcg cgtccatacc gcccatcttg tcctgatgcg cgtgagtcac
     5281 caccgccagc gcgaccggca ggttgatctc ctgcttgatc cagttgagga tctgggcggt
     5341 ctggtcatcg gtccaggcgg tatcgaccaa cagcacgcgg ccgccatccc tgacgatcaa
     5401 accgttggaa gcgactgccc cgaaacccgg catgtcgaga taggaagtgt gctgccagac
     5461 attcggtgcg agctggcgga aaaccagatc gccaaaccgt tggtcgccag tttccatttg
     5521 ctggccaatc gtcgggcgga tttcaccggg catgcacccg ctcagcatca atgcagcggc
     5581 taatgcggtg ctcagcttcg cgaccgggtg cataatattg ggcaattcca tcaagttttc
     5641 cttttattca gcattaaaaa ccccgcaaat gcgaggccta gtaaatagat gatcttaatt
     5701 tggttcactg tagcaaaaat atggggcgaa ttcaaacatg aggtgcgaca gtttcaaaag
     5761 ccatatgata atcaacaagc tgagcaaatt tctctaatgg tgtaagccaa tctaacgcct
     5821 ttctaggacg agtattcagt gacatggcaa cttgatttaa ataatgctga tctgcctgat
     5881 ttaaatcaat ccctttaggt aaatattgcc taattaaacc attcatattt tcgcatgtgc
     5941 ctttttgcca gggtgaatgt gggtcacaga aatatacatc tatgcctaaa tcttcttcga
     6001 gtattttatg tttagcactt ttctttcacc caatgggtga aagctgaatt tcgaaggaat
     6061 gcatatttat caaggctttg attatgcttt ttgaagtact gacgtagaat ctaggtatga
     6121 gtaaacggca ctgttgcaaa tagtcggtgg tgataaactt atcatcccct tttgctgatg
     6181 gagctgcaca tgaacccatt caaaggccgg cattttcagc gtgacatcat tctgtgggcc
     6241 gtacgctggt actgcaaata cggcatcagt taccgtgagc tgcaggagat gctggctgaa
     6301 cgcggagtga atgtcgatca ctccacgatt taccgctggg ttcagcgtta tgcgcctgaa
     6361 atggaaaaac ggctgcgctg gtactggcgt aacccttccg atctttgccc gtggcacatg
     6421 gatgaaacct acgtgaaggt caatggccgc tgggcgtatc tgtaccgggc cgtcgacagc
     6481 cggggccgca ctgtcgattt ttatctctcc tcccgtcgta acagcaaagc tgcataccgg
     6541 tttctgggta aaatcctcaa caacgtgaag aagtggcaga tcccgcgatt catcaacacg
     6601 gataaagcgc ccgcctatgg tcgcgcgctt gctctgctca aacgcgaagg ccggtgcccg
     6661 tctgacgttg aacaccgaca gattaagtac cggaacaacg tgattgaatg cgatcatggc
     6721 aaactgaaac ggataatcgg cgccacgctg ggatttaaat ccatgaagac ggcttacgcc
     6781 accatcaaag gtattgaggt gatgcgtgca ctacgcaaag gccaggcctc agcattttat
     6841 tatggtgatc ccctgggcga aatgcgcctg gtaagcagag tttttgaaat gtaaggcctt
     6901 tgaataagac aaaaggctgc ctcatcgcta actttgcaac agtgccctgc ggagcaaggc
     6961 cgtcgcgaac gagtggcgga gggtgtgcgg tgtggcgggc ttcgtgatgc ctgcttgttc
     7021 tacggcacgt ttgaaggcgc gctgaaaggt ctggtcatac atgtgatggc gacgcacgac
     7081 accgctccgt ggatcggtcg aatgcgtgtg ctgcgcaaaa acccagaacc acggccagga
     7141 atgcccggcg cgcggatact tccgctcaag ggcgtcggga agcgcaacgc cgctgcggcc
     7201 ctcggcctgg tccttcagcc accatgcccg tgcacgcgac agctgctcgc gcaggctggg
     7261 tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat
     7321 gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg
     7381 catgcccgtt ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag
     7441 gatgcgaacc acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct
     7501 tccgatctcc tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa
     7561 ggccgccaat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga
     7621 cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg
     7681 gatgaaggca cgaacccagt ggacataagc ctgttcggtt ggtaagctgt aatgcaagta
     7741 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     7801 agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc ctcgggcatc
     7861 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     7921 tacgcagcag ggcagtcgcc ctaaaacaaa gttagccata tgaactcgga atcagtacgc
     7981 atttatctcg ttgctgcgat gggagccaat cgggttattg gcaatggtcc taatatcccc
     8041 tggaaaattc cgggtgagca gaagattttt cgcagactca ctgagggaaa agtcgttgtc
     8101 atggggcgaa agacctttga gtctatcggc aagcctctac cgaaccgtca cacattggta
     8161 atctcacgcc aagctaacta ccgcgccact ggctgcgtag ttgtttcaac gctgtcgcac
     8221 gctatcgctt tggcatccga actcggcaat gaactctacg tcgcgggcgg agctgagata
     8281 tacactctgg cactacctca cgcccacggc gtgtttctat ctgaggtaca tcaaaccttc
     8341 gagggtgacg ccttcttccc aatgctcaac gaaacagaat tcgagcttgt ctcaaccgaa
     8401 accattcaag ctgtaattcc gtacacccac tccgtttatg cgcgtcgaaa cggctaacca
     8461 ttccgtcaac gggacgccaa aatgctgcgc attttggttc cctccgctgc gctccggctc
     8521 tcgttacgtc caacgttagc accacttaaa cccagcttta tttagctcat gtttattcaa
     8581 acggcattta gcttttcagg cgttattcag tgcctgtttt gccttttttc cgggcttcgc
     8641 ctgcatgggc tgcgcaggtt ttcagtcttt ttggcctcta gcccttgcgt agcaagcgca
     8701 agcagctatc gtttttgcag tgctgtgccg cctcggtggc gcagcgtttt ttcacggtta
     8761 gcgcccgtcg ccaaattcaa gttatccgtt ttggcttctg gttctaacat ttcggtcaag
     8821 ccgacccgca ttctgcggtc ggcttacctc gcccgttaga catcatgagg gaagcggtga
     8881 ccatcgaaat ttcgaaccaa ctatcagagg tgctaagcgt cattgagcgc catctggaat
     8941 caacgttgct ggccgtgcat ttgtacggct ccgcagtgga tggcggcctg aagccataca
     9001 gcgatattga tttgttggtt actgtggccg taaagcttga tgaaacgacg cggcgagcat
     9061 tgctcaatga tcttatggag gcttcggctt tccctggcga gagcgagacg ctccgcgcta
     9121 tagaagtcac ccttgtcgtg catgacgaca tcatcccgtg gcgttatccg gctaagcgcg
     9181 agctgcaatt tggagaatgg cagcgcaatg acattcttgc gggtatcttc gagccagcca
     9241 tgatcgacat tgatctagct atcctgctta caaaagcaag agaacatagc gttgccttgg
     9301 taggtccggc agcggaggaa ttctttgacc cggttcctga acaggatcta ttcgaggcgc
     9361 tgagggaaac cttgaagcta tggaactcgc agcccgactg ggccggcgat gagcgaaatg
     9421 tagtgcttac gttgtcccgc atttggtaca gcgcaataac cggcaaaatc gcgccgaagg
     9481 atgtcgctgc cgactgggca ataaaacgcc tacctgccca gtatcagccc gtcttacttg
     9541 aagctaagca agcttatctg ggacaaaaag aagatcactt ggcctcacgc gcagatcact
     9601 tggaagaatt tattcgcttt gtgaaaggcg agatcatcaa gtcagttggt aaatgatgtc
     9661 taacaattcg ttcaagccga ccgcgctacg cgcggcggct taactccggc gttagatgca
     9721 ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta ttatttttaa
     9781 gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg gctttttctt
     9841 gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag cgagggcttt
     9901 actaagcttg ccccttccgc cgttgtcata atcggttatg gcatcgcatt ttattttctt
     9961 tctctggttc tgaaatccat ccctgtcggt gttgcttatg cagtctggtc gggactcggc
    10021 gtcgtcataa ttacagccat tgcctggttg cttcatgggc aaaagcttga tgcgtggggc
    10081 tttgtaggta tggggctcat aattgctgcc tttttgctcg cccgatcccc atcgtggaag
    10141 tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat tctgaatctc accgaggact
    10201 ccttcttcga tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg gcgatcgaaa
    10261 tgctgcgagt cggatcagac gtcgtggatg tcggaccggc cgccagccat ccggacgcga
    10321 ggcctgtatc gccggccgat gagatcagac gtattgcgcc gctcttagac gccctgtccg
    10381 atcagatgca ccgtgtttca atcgacagct tccaaccgga aacccagcgc tatgcgctca
    10441 agcgcggcgt gggctacctg aacgatatcc aaggatttcc tgaccctgcg ctct
//
