LOCUS       LC744505               10494 bp    DNA     circular BCT 24-DEC-2022
DEFINITION  Escherichia coli MyNCGM613 plasmid pMyNCGM613 DNA, complete
            sequence.
ACCESSION   LC744505
VERSION     LC744505.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Complete plasmid sequence of multidrug-resistant Escherichia coli
            co-harboring blaNDM-5 and rmtB in Myanmar
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2022) Contact:Tomomi Hishinuma Juntendo University
            School of Medicine, Department of Microbiology; 2-1-1 Hongo,
            Bunkyo-ku, Tokyo 113-8421, Japan
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.7
            Sequencing Technology :: MinION; Illumina MiSeq
            ##Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MyNCGM613"
                     /db_xref="taxon:562"
                     /plasmid="pMyNCGM613"
                     /geo_loc_name="Myanmar"
                     /collection_date="2017"
     CDS             770..1267
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3MV56"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="BDV21603.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGKV
                     VVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVAGG
                     AEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVYARR
                     NG"
                     /name="dihydrofolate reductase"
     CDS             1675..2466
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3EQ55"
                     /codon_start=1
                     /transl_table=11
                     /product="streptomycin 3''-adenylyltransferase"
                     /protein_id="BDV21604.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKPY
                     SDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQDLF
                     EALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQP
                     VLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
                     /name="streptomycin 3''-adenylyltransferase"
     gene            2630..2977
                     /gene="ebr"
     CDS             2630..2977
                     /gene="ebr"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000679427.1"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="BDV21605.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="ebr"
     CDS             2971..3810
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3ERE4"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="BDV21606.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVG
                     PAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQ
                     GFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSALRRS
                     GVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLP
                     VKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGLDHA"
                     /name="dihydropteroate synthase"
     CDS             4215..5756
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001120888.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="BDV21607.1"
                     /translation="MSLARNATASQSPTQTNGYERHQPDQTLLYQLVEQHYPAFKASLE
                     AQGQHLPRYIQQEFNDLLQCGRLEYGFMRVRCEDCHHERLVAFSCKRRGFCPSCGARRM
                     AESAALLIDEVFPKEPIRQWVLSFPFQLRFLLARHPQLMGQVLSIVYRTLSTHLIKKAG
                     YTKASAQTGSVTLIQRFGSALNLNVHYHMLFLDGVYAEDDYGKQRFHRVKAPTYDELNT
                     LAHTLSHRIARCMEKRGILERDAENTWLTLEEGEDDTLTQLHGASVTYRIAVGPQQGRK
                     VFTLQTLPGREDKADSSSRVANHAGFSLHAGVMAEAHQRDKLERLCRYISRPAVSEKRL
                     ALTANGQVRYELKTPYRNGTTHVIFEPLDFIAKLAALVPKPRVNLTRFHGVFAPNSKHR
                     VQVTPAKRGKKPDKSEGLDTNWRDKSPAERHRAMTWMQRLKRVFNIDIEVCEHCGGHVK
                     VIASIEDPKVIEQILKHLKQKTAKANAAKQRELPPERAPPLTPSLFDPSQSRLFD"
                     /name="IS91 family transposase"
     CDS             6069..7100
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV21608.1"
                     /translation="MTASTIRLRRWLAGLALLLALPATSAVAQDFELPPVDEVFVLSAQ
                     ATAPDRIEVRWRIADGYYLYRHRTSVKADAAFTGATMALPKGKAYRDEFFGDVETYRKE
                     LLGTLTGTPAAGASATTLTVKYQGCADAGVCYPPQTRTLKVALPGEAGAGGFGCKARGL
                     HDYVVKNGSADHPNAEVKFALGDVVNTMIGCTNGETIMLCHDTSLPRPYSLGFRVQGTE
                     GLWMDVNKSIYLEGKSPQPHRWEPAEGWFAKYDHPLWKRYADLAAGAGHGGMDWFVIHA
                     FVEALKAKAPMPIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRKPIFALN
                     DAY"
                     /name="hypothetical protein"
     gene            complement(7111..7749)
                     /gene="trpF"
     CDS             complement(7111..7749)
                     /gene="trpF"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:E8TBD8"
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="BDV21609.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVAG
                     ALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKPVW
                     KALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALPWGL
                     AGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
                     /name="trpF"
     CDS             complement(7754..8119)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010920626.1"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="BDV21610.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQL
                     EFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLRIG
                     YLIDPDCTLVRLIQNPD"
                     /name="bleomycin resistance protein"
     gene            complement(8123..8935)
                     /gene="bla"
     CDS             complement(8123..8935)
                     /gene="bla"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:Q6HFY5"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="BDV21611.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQR
                     FGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLLVDTAWTDDQTAQILNW
                     IKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHSLTF
                     AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG
                     DADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
                     /name="bla"
     CDS             complement(9036..9230)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV21612.1"
                     /translation="MNGLIRQYLPKGIDLNQADQHYLNQVAMSLNTRPRKALDWLTPLE
                     KFAQLVDYHMAFETVAPHV"
                     /name="hypothetical protein"
     CDS             9476..10198
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001313567.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDV21613.1"
                     /translation="MLMELHMNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERG
                     VNVDHSTIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR
                     GRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCP
                     SDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAF
                     YYGDPLGEMRLVSRVFEM"
                     /name="IS6 family transposase"
     ARG             2631..2974
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2972..3807
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1676..2463
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             771..1264
                     /name="dfrA12"
                     /gene="dfrA12"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(8127..8935)
                     /name="blaNDM-5"
                     /gene="blaNDM-5"
                     /gene_name="subclass B1 metallo-beta-lactamase NDM-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7758..8119)
                     /name="ble"
                     /gene="ble"
                     /gene_name="bleomycin binding protein Ble-MBL"
                     /tool="amrfinderplus"
                     /drug_class="BLEOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             1688..2466
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(7755..8119)
                     /name="BRP(MBL)"
                     /gene="BRP(MBL)"
                     /gene_name="Bleomycin resistant protein"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 cgctgcggcc ctcggcctgg tccttcagcc accatgcccg tgcacgcgac agctgctcgc
       61 gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct
      121 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct
      181 cactgatccg catgcccgtt ccatacagaa gctgggcgaa caaacgatgc tcgccttcca
      241 gaaaaccgag gatgcgaacc acttcatccg gggtcagcac caccggcaag cgccgcgacg
      301 gccgaggtct tccgatctcc tgaagccagg gcagatccgt gcacagcacc ttgccgtaga
      361 agaacagcaa ggccgccaat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg
      421 ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac
      481 cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt ggtaagctgt
      541 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
      601 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
      661 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      721 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttagccata tgaactcgga
      781 atcagtacgc atttatctcg ttgctgcgat gggagccaat cgggttattg gcaatggtcc
      841 taatatcccc tggaaaattc cgggtgagca gaagattttt cgcagactca ctgagggaaa
      901 agtcgttgtc atggggcgaa agacctttga gtctatcggc aagcctctac cgaaccgtca
      961 cacattggta atctcacgcc aagctaacta ccgcgccact ggctgcgtag ttgtttcaac
     1021 gctgtcgcac gctatcgctt tggcatccga actcggcaat gaactctacg tcgcgggcgg
     1081 agctgagata tacactctgg cactacctca cgcccacggc gtgtttctat ctgaggtaca
     1141 tcaaaccttc gagggtgacg ccttcttccc aatgctcaac gaaacagaat tcgagcttgt
     1201 ctcaaccgaa accattcaag ctgtaattcc gtacacccac tccgtttatg cgcgtcgaaa
     1261 cggctaacca ttccgtcaac gggacgccaa aatgctgcgc attttggttc cctccgctgc
     1321 gctccggctc tcgttacgtc caacgttagc accacttaaa cccagcttta tttagctcat
     1381 gtttattcaa acggcattta gcttttcagg cgttattcag tgcctgtttt gccttttttc
     1441 cgggcttcgc ctgcatgggc tgcgcaggtt ttcagtcttt ttggcctcta gcccttgcgt
     1501 agcaagcgca agcagctatc gtttttgcag tgctgtgccg cctcggtggc gcagcgtttt
     1561 ttcacggtta gcgcccgtcg ccaaattcaa gttatccgtt ttggcttctg gttctaacat
     1621 ttcggtcaag ccgacccgca ttctgcggtc ggcttacctc gcccgttaga catcatgagg
     1681 gaagcggtga ccatcgaaat ttcgaaccaa ctatcagagg tgctaagcgt cattgagcgc
     1741 catctggaat caacgttgct ggccgtgcat ttgtacggct ccgcagtgga tggcggcctg
     1801 aagccataca gcgatattga tttgttggtt actgtggccg taaagcttga tgaaacgacg
     1861 cggcgagcat tgctcaatga tcttatggag gcttcggctt tccctggcga gagcgagacg
     1921 ctccgcgcta tagaagtcac ccttgtcgtg catgacgaca tcatcccgtg gcgttatccg
     1981 gctaagcgcg agctgcaatt tggagaatgg cagcgcaatg acattcttgc gggtatcttc
     2041 gagccagcca tgatcgacat tgatctagct atcctgctta caaaagcaag agaacatagc
     2101 gttgccttgg taggtccggc agcggaggaa ttctttgacc cggttcctga acaggatcta
     2161 ttcgaggcgc tgagggaaac cttgaagcta tggaactcgc agcccgactg ggccggcgat
     2221 gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcaataac cggcaaaatc
     2281 gcgccgaagg atgtcgctgc cgactgggca ataaaacgcc tacctgccca gtatcagccc
     2341 gtcttacttg aagctaagca agcttatctg ggacaaaaag aagatcactt ggcctcacgc
     2401 gcagatcact tggaagaatt tattcgcttt gtgaaaggcg agatcatcaa gtcagttggt
     2461 aaatgatgtc taacaattcg ttcaagccga ccgcgctacg cgcggcggct taactccggc
     2521 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     2581 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg
     2641 gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag
     2701 cgagggcttt actaagcttg ccccttccgc cgttgtcata atcggttatg gcatcgcatt
     2761 ttattttctt tctctggttc tgaaatccat ccctgtcggt gttgcttatg cagtctggtc
     2821 gggactcggc gtcgtcataa ttacagccat tgcctggttg cttcatgggc aaaagcttga
     2881 tgcgtggggc tttgtaggta tggggctcat aattgctgcc tttttgctcg cccgatcccc
     2941 atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat tctgaatctc
     3001 accgaggact ccttcttcga tgagagccgg cggctagacc ccgccggcgc tgtcaccgcg
     3061 gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg tcggaccggc cgccagccat
     3121 ccggacgcga ggcctgtatc gccggccgat gagatcagac gtattgcgcc gctcttagac
     3181 gccctgtccg atcagatgca ccgtgtttca atcgacagct tccaaccgga aacccagcgc
     3241 tatgcgctca agcgcggcgt gggctacctg aacgatatcc aaggatttcc tgaccctgcg
     3301 ctctatcccg atattgctga ggcggactgc aggctggtgg ttatgcactc agcgcagcgg
     3361 gatggcatcg ccacccgcac cggtcacctt cgacccgaag acgcgctcga cgagattgtg
     3421 cggttcttcg aggcgcgggt ttccgccttg cgacggagcg gggtcgctgc cgaccggctc
     3481 atcctcgatc cggggatggg atttttcttg agccccgcac cggaaacatc gctgcacgtg
     3541 ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc cgctattggt ctcggtgtcg
     3601 cggaaatcct tcttgggcgc caccgttggc cttcctgtaa aggatctggg tccagcgagc
     3661 cttgcggcgg aacttcacgc gatcggcaat ggcgctgact acgtccgcac ccacgcgcct
     3721 ggagatctgc gaagcgcaat caccttctcg gaaaccctcg cgaaatttcg cagtcgcgac
     3781 gccagagacc gagggttaga tcatgcctag cattcacctt ccggccgccc gctaaatatc
     3841 tccttttggg ttgttaataa aacatccaat aagttgactg tgcgtgaaaa agaaagtttt
     3901 gtgtgatggc gttgaagatc gcaccgttaa gctcttatgt gggatggtgc agagctcgac
     3961 gactaccgat aaaacgcaac cgccgcaaac agacaagaaa aagccccaac tgataacagt
     4021 tggggcttca gtattgtgat tggtggagca atagcaccct gaacccaaaa ccttctcgct
     4081 caaccggtag tggctgataa caactcgtga gggctattgc gggttaagca tttagcgatg
     4141 tctagggcca gactggacgt ctgaacgcaa gccgctgata ctgtacataa ccacagtatc
     4201 agcggaggat acccatgtcg ctggcaagga acgccacggc gagtcaatcg cccactcaaa
     4261 caaacggtta cgaacgccac caacccgacc agacgctgct ctaccagctg gttgagcagc
     4321 actacccagc cttcaaagcc tcactcgaag cccaaggtca acacctgcct cgctacatcc
     4381 aacaagaatt caacgacctc ctccaatgtg gccgtctgga gtatggtttc atgcgggttc
     4441 gctgcgagga ttgtcatcac gagcgtctgg tcgccttcag ctgtaaacga cgcggctttt
     4501 gccctagctg cggtgcccgc cggatggccg agagtgcggc gctgctgata gacgaagtct
     4561 tccccaagga gcccattcgc cagtgggtgc tcagctttcc tttccagcta cgctttttgc
     4621 tggctcgcca tccccagctg atgggccagg tcttgagtat cgtctatcgt acactctcaa
     4681 ctcatctgat caaaaaagcc ggttacacca aagcctctgc acaaactggc tcagtgactc
     4741 ttatccaacg ctttggctcc gcgctaaatc tcaatgtcca ctaccacatg ctgtttctcg
     4801 atggtgtcta tgccgaagat gactatggca agcaacgctt ccatcgtgtc aaggcaccca
     4861 cttacgatga gctgaatacg ctcgctcaca ccctcagcca tcgcatcgct cgctgcatgg
     4921 aaaagcgtgg gattttggag cgtgatgccg agaatacgtg gttgacactg gaagagggcg
     4981 aagacgatac gctgactcaa ttacatggtg cttcggttac gtatcgcatt gccgtcggcc
     5041 cccagcaagg gcgcaaagtc ttcaccctgc aaaccttgcc agggcgtgag gataaagccg
     5101 actcaagcag tcgagtagcc aaccatgctg gtttctcgct acacgccggt gtgatggccg
     5161 aagcgcatca gcgggataag cttgagcgct tgtgtcgcta cattagtcgg ccagcggttt
     5221 cagaaaaacg tctggcatta accgccaatg ggcaggtgcg ttacgagctc aaaactccgt
     5281 accgcaatgg caccacccat gtgatcttcg agccgctgga cttcatcgcc aaactcgctg
     5341 cgttggtacc taagccgcga gtcaacctca cacgcttcca cggcgtcttt gcaccgaaca
     5401 gcaaacaccg agttcaagta acacccgcca agcggggcaa gaagcccgac aaatcggaag
     5461 gtctcgatac taactggcgt gacaagagtc ctgcagagcg ccaccgcgcc atgacctgga
     5521 tgcaacgcct caagcgagtc ttcaatattg atattgaagt ctgcgaacac tgcggcggtc
     5581 acgtcaaagt gattgccagc atcgaagatc cgaaggtcat tgagcagatt ctcaagcatc
     5641 tgaaacagaa aacagccaag gcgaatgccg ccaagcagcg tgagctgcca ccagaacgag
     5701 cgccgccact gactcccagc ctgttcgatc catcacagag tcgtctcttt gactgacgac
     5761 cccaaatcca acactgctca acactgccaa cttttaaacg gggcggtggg gcagtttgta
     5821 tctctcgagc tatcaggcta gagattttac cgccaaatcg aaccttatta gagcggttta
     5881 ggctggaccg gcagttaaaa ttggggcttg agcggtaaac gagtgaggga atttcaggta
     5941 agatacttcg gatgaggagc aaaaaggtgg tttatacttc ctatacccgc tcgcggtcga
     6001 agccgcgtcc ggcctgcccg aatacctgca atggctggcc gccgagagcc gaccggtaaa
     6061 ctgagccaat gaccgcatcc acgatccgcc tgcgccgctg gctggccggg ctcgccctgc
     6121 tgcttgcgct gcccgcgacc tcggccgtcg cccaggactt cgaactgccg ccggtcgacg
     6181 aggtcttcgt cctgtccgcg caggccaccg cgccggaccg catcgaggtg cgctggcgga
     6241 tcgccgacgg ctattacctg taccggcacc gcacctcggt caaggccgat gccgccttca
     6301 ccggcgcgac catggcgctg ccgaagggca aggcctaccg cgacgaattc ttcggcgacg
     6361 tcgaaaccta ccgcaaggaa ttgctcggca ccctcaccgg cacgcccgcg gccggcgcga
     6421 gcgcgaccac cctgaccgtg aagtaccagg gctgcgccga tgccggcgtg tgctacccgc
     6481 cgcagacccg caccctgaag gtcgcgttgc cgggcgaagc gggcgctggc ggcttcggct
     6541 gtaaggcgcg cgggctgcat gattatgtcg tcaagaacgg ctctgccgat catcccaacg
     6601 ccgaggtgaa attcgcactg ggtgatgtgg tcaacaccat gatcggctgc actaatggtg
     6661 aaacgatcat gctgtgccac gacacctcgc tgccgcgccc ctattctctc ggctttcggg
     6721 tgcaaggcac cgaggggctg tggatggacg tcaacaagtc gatctatctg gagggcaaga
     6781 gcccacagcc gcaccgctgg gagcctgccg agggctggtt tgcgaaatac gatcacccgc
     6841 tatggaaacg ctacgccgat ctggcggcag gggccgggca tggcgggatg gactggttcg
     6901 tgatccacgc ttttgtcgag gcgctgaagg ccaaggcccc gatgccaatc gacatttacg
     6961 acgcgctggc ctggagcgcg atcacgcctc tgtcggaaca atcgattgct gagggcaatc
     7021 gcacgttaga ttttcccgac ttcacccgag ggcagtggcg cacccgcaag ccgatctttg
     7081 cgctgaacga cgcctattga tcgacgcgat ttaggccaag cgcaccgcaa aggcgaaatt
     7141 ggtaatcttg tcggtatcct tgacgcccgg cgcgctttcg acgccgctgg aggtatcgac
     7201 cagcggcgct ccggtgcgcg caatcgcctc ggcaacattc gtcggattta gcccgcctgc
     7261 cagcccccac ggcaaggcac cgcgatatcc ggccagcagc gaccagtcga acgccaaccc
     7321 catgccgccg ggcagcgcgc ctttgggggt cttggcgtcg aacaagatca agtccgccgc
     7381 cccggcatag gctgcggcgc gtgcgacatc gctggcgctg gcgacgggca gcgccttcca
     7441 caccggcttg ccaaaccgcg cgcgcaactg ggccacgcgt tcgggcgatt ccgaaccgtg
     7501 cagctgcagc gcgttcagct tggctgccac cagtgcgtcg gcgatgacag catcatccgc
     7561 atcgacgaac aaaccgacca tggcgatctg gccagctgcg cgcgatgtca aagcgcccgc
     7621 gacattcgac gtaaccgcac ggggcgacgc tggatagaac accaacccgg catagtccgc
     7681 ccgcgccgcg atggtcgcat cgagcgcctc gggtgtgctg atcccgcaaa tcttgatttt
     7741 cgcgggcatg cggtcagtcg gggttctgga tcagccgcac cagcgtgcag tcgggatcga
     7801 tcaggtagcc gatcctcagg ccgctcgcct ccagttgcgg agctttgaag cgcggccagc
     7861 cggtgctttt ttcctcggct cccgccgcgt tcaccaatgc caccatggca tcgagatcat
     7921 ccaaccgcag gcaacagccg aacgagctcg tagctgggtc gaggtcagga taggggaaga
     7981 attcgagctg caaaccgccg cgctgcagga tcatccagcc gcgatccttc caactcgtcg
     8041 caaagcccag cttcgcataa aacgcctctg tcacatcgaa atcgcgcgat ggcagattgg
     8101 gggtgacgtg gtcagccatg gctcagcgca gcttgtcggc catgcgggcc gtatgagtga
     8161 ttgcggcgcg gctatcgggg gcggaatggc tcatcacgat catgctggcc ttggggaacg
     8221 ccgcaccaaa cgcgcgcgct gacgcggcgt agtgctcagt gtcggcatca ccgagattgc
     8281 cgagcgactt ggccttgctg tccttgatca ggcagccacc aaaagcgatg tcggtgccgt
     8341 cgatcccaac ggtgatattg tcactggtgt ggccggggcc ggggtaaaat accttgagcg
     8401 ggccaaagtt gggcgcggtt gctggttcga cccagccatt ggcggcgaaa gtcaggctgt
     8461 gttgcgccgc aaccagcccc tcttgcgggg caagctggtt cgacaacgca ttggcataag
     8521 tcgcaatccc cgccgcatgc agcgcgtcca taccgcccat cttgtcctga tgcgcgtgag
     8581 tcaccaccgc cagcgcgacc ggcaggttga tctcctgctt gatccagttg aggatctggg
     8641 cggtctggtc atcggtccag gcggtatcga ccaacagcac gcggccgcca tccctgacga
     8701 tcaaaccgtt ggaagcgact gccccgaaac ccggcatgtc gagataggaa gtgtgctgcc
     8761 agacattcgg tgcgagctgg cggaaaacca gatcgccaaa ccgttggtcg ccagtttcca
     8821 tttgctggcc aatcgtcggg cggatttcac cgggcatgca cccgctcagc atcaatgcag
     8881 cggctaatgc ggtgctcagc ttcgcgaccg ggtgcataat attgggcaat tccatcaagt
     8941 tttcctttta ttcagcatta aaaaccccgc aaatgcgagg cctagtaaat agatgatctt
     9001 aatttggttc actgtagcaa aaatatgggg cgaattcaaa catgaggtgc gacagtttca
     9061 aaagccatat gataatcaac aagctgagca aatttctcta atggtgtaag ccaatctaac
     9121 gcctttctag gacgagtatt cagtgacatg gcaacttgat ttaaataatg ctgatctgcc
     9181 tgatttaaat caatcccttt aggtaaatat tgcctaatta aaccattcat attttcgcat
     9241 gtgccttttt gccagggtga atgtgggtca cagaaatata catctatgcc taaatcttct
     9301 tcgagtattt tatgttgagc acttttcttt cacccaatgg gtgaaagctg aatttcgaag
     9361 gaatgcatat ttatcaaggc tttgattatg ctttttgaag tactgacgta gaatctaggt
     9421 atgagtaaac ggcactgttg caaatagtcg gtggtgataa acttatcatc cccttttgct
     9481 gatggagctg cacatgaacc cattcaaagg ccggcatttt cagcgtgaca tcattctgtg
     9541 ggccgtacgc tggtactgca aatacggcat cagttaccgt gagctgcagg agatgctggc
     9601 tgaacgcgga gtgaatgtcg atcactccac gatttaccgc tgggttcagc gttatgcgcc
     9661 tgaaatggaa aaacggctgc gctggtactg gcgtaaccct tccgatcttt gcccgtggca
     9721 catggatgaa acctacgtga aggtcaatgg ccgctgggcg tatctgtacc gggccgtcga
     9781 cagccggggc cgcactgtcg atttttatct ctcctcccgt cgtaacagca aagctgcata
     9841 ccggtttctg ggtaaaatcc tcaacaacgt gaagaagtgg cagatcccgc gattcatcaa
     9901 cacggataaa gcgcccgcct atggtcgcgc gcttgctctg ctcaaacgcg aaggccggtg
     9961 cccgtctgac gttgaacacc gacagattaa gtaccggaac aacgtgattg aatgcgatca
    10021 tggcaaactg aaacggataa tcggcgccac gctgggattt aaatccatga agacggctta
    10081 cgccaccatc aaaggtattg aggtgatgcg tgcactacgc aaaggccagg cctcagcatt
    10141 ttattatggt gatcccctgg gcgaaatgcg cctggtaagc agagtttttg aaatgtaagg
    10201 cctttgaata agacaaaagg ctgcctcatc gctaactttg caacagtgcc ctgcggagca
    10261 aggccgtcgc gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt
    10321 gttctacggc acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca
    10381 cgacaccgct ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc
    10441 aggaatgccc ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgc
//
