LOCUS       LC744501               10494 bp    DNA     circular BCT 24-DEC-2022
DEFINITION  Escherichia coli MyNCGM600 plasmid pMyNCGM600 DNA, complete
            sequence.
ACCESSION   LC744501
VERSION     LC744501.1
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Complete plasmid sequence of multidrug-resistant Escherichia coli
            co-harboring blaNDM-5 and rmtB in Myanmar
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Hishinuma,T., Tada,T. and Kirikae,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2022) Contact:Tomomi Hishinuma Juntendo University
            School of Medicine, Department of Microbiology; 2-1-1 Hongo,
            Bunkyo-ku, Tokyo 113-8421, Japan
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.7
            Sequencing Technology :: MinION; Illumina MiSeq
            ##Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MyNCGM600"
                     /db_xref="taxon:562"
                     /plasmid="pMyNCGM600"
                     /geo_loc_name="Myanmar"
                     /collection_date="2017"
     CDS             complement(1805..2644)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3ERE4"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="BDV20732.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVG
                     PAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQ
                     GFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSALRRS
                     GVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGATVGLP
                     VKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGLDHA"
                     /name="dihydropteroate synthase"
     gene            complement(2638..2985)
                     /gene="ebr"
     CDS             complement(2638..2985)
                     /gene="ebr"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000679427.1"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="BDV20733.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY
                     FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP
                     SWKSLRRPTPW"
                     /name="ebr"
     CDS             complement(3149..3940)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3EQ55"
                     /codon_start=1
                     /transl_table=11
                     /product="streptomycin 3''-adenylyltransferase"
                     /protein_id="BDV20734.1"
                     /translation="MREAVTIEISNQLSEVLSVIERHLESTLLAVHLYGSAVDGGLKPY
                     SDIDLLVTVAVKLDETTRRALLNDLMEASAFPGESETLRAIEVTLVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPAMIDIDLAILLTKAREHSVALVGPAAEEFFDPVPEQDLF
                     EALRETLKLWNSQPDWAGDERNVVLTLSRIWYSAITGKIAPKDVAADWAIKRLPAQYQP
                     VLLEAKQAYLGQKEDHLASRADHLEEFIRFVKGEIIKSVGK"
                     /name="streptomycin 3''-adenylyltransferase"
     CDS             complement(4348..4845)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A0A0H3MV56"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="BDV20735.1"
                     /translation="MNSESVRIYLVAAMGANRVIGNGPNIPWKIPGEQKIFRRLTEGKV
                     VVMGRKTFESIGKPLPNRHTLVISRQANYRATGCVVVSTLSHAIALASELGNELYVAGG
                     AEIYTLALPHAHGVFLSEVHQTFEGDAFFPMLNETEFELVSTETIQAVIPYTHSVYARR
                     NG"
                     /name="dihydrofolate reductase"
     CDS             4990..5877
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000845048.1"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="BDV20736.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLPTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRRALLQS"
                     /name="integron integrase"
     CDS             complement(5911..6633)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001313567.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDV20737.1"
                     /translation="MLMELHMNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERG
                     VNVDHSTIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSR
                     GRTVDFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCP
                     SDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAF
                     YYGDPLGEMRLVSRVFEM"
                     /name="IS6 family transposase"
     CDS             6879..7073
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV20738.1"
                     /translation="MNGLIRQYLPKGIDLNQADQHYLNQVAMSLNTRPRKALDWLTPLE
                     KFAQLVDYHMAFETVAPHV"
                     /name="hypothetical protein"
     gene            7174..7986
                     /gene="bla"
     CDS             7174..7986
                     /gene="bla"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:Q6HFY5"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="BDV20739.1"
                     /translation="MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQR
                     FGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLLVDTAWTDDQTAQILNW
                     IKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGLVAAQHSLTF
                     AANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLG
                     DADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR"
                     /name="bla"
     CDS             7990..8355
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010920626.1"
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin resistance protein"
                     /protein_id="BDV20740.1"
                     /translation="MADHVTPNLPSRDFDVTEAFYAKLGFATSWKDRGWMILQRGGLQL
                     EFFPYPDLDPATSSFGCCLRLDDLDAMVALVNAAGAEEKSTGWPRFKAPQLEASGLRIG
                     YLIDPDCTLVRLIQNPD"
                     /name="bleomycin resistance protein"
     gene            8360..8998
                     /gene="trpF"
     CDS             8360..8998
                     /gene="trpF"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:E8TBD8"
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="BDV20741.1"
                     /translation="MPAKIKICGISTPEALDATIAARADYAGLVFYPASPRAVTSNVAG
                     ALTSRAAGQIAMVGLFVDADDAVIADALVAAKLNALQLHGSESPERVAQLRARFGKPVW
                     KALPVASASDVARAAAYAGAADLILFDAKTPKGALPGGMGLAFDWSLLAGYRGALPWGL
                     AGGLNPTNVAEAIARTGAPLVDTSSGVESAPGVKDTDKITNFAFAVRLA"
                     /name="trpF"
     CDS             complement(9009..10040)
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDV20742.1"
                     /translation="MTASTIRLRRWLAGLALLLALPATSAVAQDFELPPVDEVFVLSAQ
                     ATAPDRIEVRWRIADGYYLYRHRTSVKADAAFTGATMALPKGKAYRDEFFGDVETYRKE
                     LLGTLTGTPAAGASATTLTVKYQGCADAGVCYPPQTRTLKVALPGEAGAGGFGCKARGL
                     HDYVVKNGSADHPNAEVKFALGDVVNTMIGCTNGETIMLCHDTSLPRPYSLGFRVQGTE
                     GLWMDVNKSIYLEGKSPQPHRWEPAEGWFAKYDHPLWKRYADLAAGAGHGGMDWFVIHA
                     FVEALKAKAPMPIDIYDALAWSAITPLSEQSIAEGNRTLDFPDFTRGQWRTRKPIFALN
                     DAY"
                     /name="hypothetical protein"
     ARG             complement(2642..2985)
                     /name="qacEdelta1"
                     /gene="qacEdelta1"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1809..2644)
                     /name="sul1"
                     /gene="sul1"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3153..3940)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4352..4845)
                     /name="dfrA12"
                     /gene="dfrA12"
                     /gene_name="trimethoprim-resistant dihydrofolate reductase
                     DfrA12"
                     /tool="amrfinderplus"
                     /drug_class="TRIMETHOPRIM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7175..7983
                     /name="blaNDM-5"
                     /gene="blaNDM-5"
                     /gene_name="subclass B1 metallo-beta-lactamase NDM-5"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7991..8352
                     /name="ble"
                     /gene="ble"
                     /gene_name="bleomycin binding protein Ble-MBL"
                     /tool="amrfinderplus"
                     /drug_class="BLEOMYCIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3150..3928)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             7991..8355
                     /name="BRP(MBL)"
                     /gene="BRP(MBL)"
                     /gene_name="Bleomycin resistant protein"
                     /tool="rgi"
                     /drug_class="glycopeptide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ttcggatctt cgatgctggc aatcactttg acgtgaccgc cgcagtgttc gcagacttca
       61 atatcaatat tgaagactcg cttgaggcgt tgcatccagg tcatggcgcg gtggcgctct
      121 gcaggactct tgtcacgcca gttagtatcg agaccttccg atttgtcggg cttcttgccc
      181 cgcttggcgg gtgttacttg aactcggtgt ttgctgttcg gtgcaaagac gccgtggaag
      241 cgtgtgaggt tgactcgcgg cttaggtacc aacgcagcga gtttggcgat gaagtccagc
      301 ggctcgaaga tcacatgggt ggtgccattg cggtacggag ttttgagctc gtaacgcacc
      361 tgcccattgg cggttaatgc cagacgtttt tctgaaaccg ctggccgact aatgtagcga
      421 cacaagcgct caagcttatc ccgctgatgc gcttcggcca tcacaccggc gtgtagcgag
      481 aaaccagcat ggttggctac tcgactgctt gagtcggctt tatcctcacg ccctggcaag
      541 gtttgcaggg tgaagacttt gcgcccttgc tgggggccga cggcaatgcg atacgtaacc
      601 gaagcaccat gtaattgagt cagcgtatcg tcttcgccct cttccagtgt caaccacgta
      661 ttctcggcat cacgctccaa aatcccacgc ttttccatgc agcgagcgat gcgatggctg
      721 agggtgtgag cgagcgtatt cagctcatcg taagtgggtg ccttgacacg atggaagcgt
      781 tgcttgccat agtcatcttc ggcatagaca ccatcgagaa acagcatgtg gtagtggaca
      841 ttgagattta gcgcggagcc aaagcgttgg ataagagtca ctgagccagt ttgtgcagag
      901 gctttggtgt aaccggcttt tttgatcaga tgagttgaga gtgtacgata gacgatactc
      961 aagacctggc ccatcagctg gggatggcga gccagcaaaa agcgtagctg gaaaggaaag
     1021 ctgagcaccc actggcgaat gggctccttg gggaagactt cgtctatcag cagcgccgca
     1081 ctctcggcca tccggcgggc accgcagcta gggcaaaagc cgcgtcgttt acagctgaag
     1141 gcgaccagac gctcgtgatg acaatcctcg cagcgaaccc gcatgaaacc atactccaga
     1201 cggccacatt ggaggaggtc gttgaattct tgttggatgt agcgaggcag gtgttgacct
     1261 tgggcttcga gtgaggcttt gaaggctggg tagtgctgct caaccagctg gtagagcagc
     1321 gtctggtcgg gttggtggcg ttcgtaaccg tttgtttgag tgggcgattg actcgccgtg
     1381 gcgttccttg ccagcgacat gggtatcctc cgctgatact gtggttatgt acagtatcag
     1441 cggcttgcgt tcagacgtcc agtctggccc tagacatcgc taaatgctta acccgcaata
     1501 gccctcacga gttgttatca gccactaccg gttgagcgag aaggttttgg gttcagggtg
     1561 ctattgctcc accaatcaca atactgaagc cccaactgtt atcagttggg gctttttctt
     1621 gtctgtttgc ggcggttgcg ttttatcggt agtcgtcgag ctctgcacca tcccacataa
     1681 gagcttaacg gtgcgatctt caacgccatc acacaaaact ttctttttca cgcacagtca
     1741 acttattgga tgttttatta acaacccaaa aggagatatt tagcgggcgg ccggaaggtg
     1801 aatgctaggc atgatctaac cctcggtctc tggcgtcgcg actgcgaaat ttcgcgaggg
     1861 tttccgagaa ggtgattgcg cttcgcagat ctccaggcgc gtgggtgcgg acgtagtcag
     1921 cgccattgcc gatcgcgtga agttccgccg caaggctcgc tggacccaga tcctttacag
     1981 gaaggccaac ggtggcgccc aagaaggatt tccgcgacac cgagaccaat agcggaagcc
     2041 ccaacgccga cttcagcttt tgaaggttcg acagcacgtg cagcgatgtt tccggtgcgg
     2101 ggctcaagaa aaatcccatc cccggatcga ggatgagccg gtcggcagcg accccgctcc
     2161 gtcgcaaggc ggaaacccgc gcctcgaaga accgcacaat ctcgtcgagc gcgtcttcgg
     2221 gtcgaaggtg accggtgcgg gtggcgatgc catcccgctg cgctgagtgc ataaccacca
     2281 gcctgcagtc cgcctcagca atatcgggat agagcgcagg gtcaggaaat ccttggatat
     2341 cgttcaggta gcccacgccg cgcttgagcg catagcgctg ggtttccggt tggaagctgt
     2401 cgattgaaac acggtgcatc tgatcggaca gggcgtctaa gagcggcgca atacgtctga
     2461 tctcatcggc cggcgataca ggcctcgcgt ccggatggct ggcggccggt ccgacatcca
     2521 cgacgtctga tccgactcgc agcatttcga tcgccgcggt gacagcgccg gcggggtcta
     2581 gccgccggct ctcatcgaag aaggagtcct cggtgagatt cagaatgccg aacaccgtca
     2641 ccatggcgtc ggcctccgca gcgacttcca cgatggggat cgggcgagca aaaaggcagc
     2701 aattatgagc cccataccta caaagcccca cgcatcaagc ttttgcccat gaagcaacca
     2761 ggcaatggct gtaattatga cgacgccgag tcccgaccag actgcataag caacaccgac
     2821 agggatggat ttcagaacca gagaaagaaa ataaaatgcg atgccataac cgattatgac
     2881 aacggcggaa ggggcaagct tagtaaagcc ctcgctagat tttaatgcgg atgttgcgat
     2941 tacttcgcca actattgcga taacaagaaa aagccagcct ttcatgatat atctcccaat
     3001 ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac ctgatagttt
     3061 ggctgtgagc aattatgtgc ttagtgcatc taacgccgga gttaagccgc cgcgcgtagc
     3121 gcggtcggct tgaacgaatt gttagacatc atttaccaac tgacttgatg atctcgcctt
     3181 tcacaaagcg aataaattct tccaagtgat ctgcgcgtga ggccaagtga tcttcttttt
     3241 gtcccagata agcttgctta gcttcaagta agacgggctg atactgggca ggtaggcgtt
     3301 ttattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttatt gcgctgtacc
     3361 aaatgcggga caacgtaagc actacatttc gctcatcgcc ggcccagtcg ggctgcgagt
     3421 tccatagctt caaggtttcc ctcagcgcct cgaatagatc ctgttcagga accgggtcaa
     3481 agaattcctc cgctgccgga cctaccaagg caacgctatg ttctcttgct tttgtaagca
     3541 ggatagctag atcaatgtcg atcatggctg gctcgaagat acccgcaaga atgtcattgc
     3601 gctgccattc tccaaattgc agctcgcgct tagccggata acgccacggg atgatgtcgt
     3661 catgcacgac aagggtgact tctatagcgc ggagcgtctc gctctcgcca gggaaagccg
     3721 aagcctccat aagatcattg agcaatgctc gccgcgtcgt ttcatcaagc tttacggcca
     3781 cagtaaccaa caaatcaata tcgctgtatg gcttcaggcc gccatccact gcggagccgt
     3841 acaaatgcac ggccagcaac gttgattcca gatggcgctc aatgacgctt agcacctctg
     3901 atagttggtt cgaaatttcg atggtcaccg cttccctcat gatgtctaac gggcgaggta
     3961 agccgaccgc agaatgcggg tcggcttgac cgaaatgtta gaaccagaag ccaaaacgga
     4021 taacttgaat ttggcgacgg gcgctaaccg tgaaaaaacg ctgcgccacc gaggcggcac
     4081 agcactgcaa aaacgatagc tgcttgcgct tgctacgcaa gggctagagg ccaaaaagac
     4141 tgaaaacctg cgcagcccat gcaggcgaag cccggaaaaa aggcaaaaca ggcactgaat
     4201 aacgcctgaa aagctaaatg ccgtttgaat aaacatgagc taaataaagc tgggtttaag
     4261 tggtgctaac gttggacgta acgagagccg gagcgcagcg gagggaacca aaatgcgcag
     4321 cattttggcg tcccgttgac ggaatggtta gccgtttcga cgcgcataaa cggagtgggt
     4381 gtacggaatt acagcttgaa tggtttcggt tgagacaagc tcgaattctg tttcgttgag
     4441 cattgggaag aaggcgtcac cctcgaaggt ttgatgtacc tcagatagaa acacgccgtg
     4501 ggcgtgaggt agtgccagag tgtatatctc agctccgccc gcgacgtaga gttcattgcc
     4561 gagttcggat gccaaagcga tagcgtgcga cagcgttgaa acaactacgc agccagtggc
     4621 gcggtagtta gcttggcgtg agattaccaa tgtgtgacgg ttcggtagag gcttgccgat
     4681 agactcaaag gtctttcgcc ccatgacaac gacttttccc tcagtgagtc tgcgaaaaat
     4741 cttctgctca cccggaattt tccaggggat attaggacca ttgccaataa cccgattggc
     4801 tcccatcgca gcaacgagat aaatgcgtac tgattccgag ttcatatggc taactttgtt
     4861 ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc
     4921 acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca
     4981 taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg
     5041 gaccagttgc gtgagcgcat acgctacttg cattacagct taccaaccga acaggcttat
     5101 gtccactggg ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc
     5161 agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc
     5221 acgcatcgtc aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat
     5281 ctgccctggc ttcaggagat cggaagacct cggccgtcgc ggcgcttgcc ggtggtgctg
     5341 accccggatg aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc
     5401 cagcttctgt atggaacggg catgcggatc agtgagggtt tgcaactgcg ggtcaaggat
     5461 ctggatttcg atcacggcac gatcatcgtg cgggagggca agggctccaa ggatcgggcc
     5521 ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc agctgtcgcg tgcacgggca
     5581 tggtggctga aggaccaggc cgagggccgc agcggcgttg cgcttcccga cgcccttgag
     5641 cggaagtatc cgcgcgccgg gcattcctgg ccgtggttct gggtttttgc gcagcacacg
     5701 cattcgaccg atccacggag cggtgtcgtg cgtcgccatc acatgtatga ccagaccttt
     5761 cagcgcgcct tcaaacgtgc cgtagaacaa gcaggcatca cgaagcccgc cacaccgcac
     5821 accctccgcc actcgttcgc gacggccttg ctccgcaggg cactgttgca aagttagcga
     5881 tgaggcagcc ttttgtctta ttcaaaggcc ttacatttca aaaactctgc ttaccaggcg
     5941 catttcgccc aggggatcac cataataaaa tgctgaggcc tggcctttgc gtagtgcacg
     6001 catcacctca atacctttga tggtggcgta agccgtcttc atggatttaa atcccagcgt
     6061 ggcgccgatt atccgtttca gtttgccatg atcgcattca atcacgttgt tccggtactt
     6121 aatctgtcgg tgttcaacgt cagacgggca ccggccttcg cgtttgagca gagcaagcgc
     6181 gcgaccatag gcgggcgctt tatccgtgtt gatgaatcgc gggatctgcc acttcttcac
     6241 gttgttgagg attttaccca gaaaccggta tgcagctttg ctgttacgac gggaggagag
     6301 ataaaaatcg acagtgcggc cccggctgtc gacggcccgg tacagatacg cccagcggcc
     6361 attgaccttc acgtaggttt catccatgtg ccacgggcaa agatcggaag ggttacgcca
     6421 gtaccagcgc agccgttttt ccatttcagg cgcataacgc tgaacccagc ggtaaatcgt
     6481 ggagtgatcg acattcactc cgcgttcagc cagcatctcc tgcagctcac ggtaactgat
     6541 gccgtatttg cagtaccagc gtacggccca cagaatgatg tcacgctgaa aatgccggcc
     6601 tttgaatggg ttcatgtgca gctccatcag caaaagggga tgataagttt atcaccaccg
     6661 actatttgca acagtgccgt ttactcatac ctagattcta cgtcagtact tcaaaaagca
     6721 taatcaaagc cttgataaat atgcattcct tcgaaattca gctttcaccc attgggtgaa
     6781 agaaaagtgc tcaacataaa atactcgaag aagatttagg catagatgta tatttctgtg
     6841 acccacattc accctggcaa aaaggcacat gcgaaaatat gaatggttta attaggcaat
     6901 atttacctaa agggattgat ttaaatcagg cagatcagca ttatttaaat caagttgcca
     6961 tgtcactgaa tactcgtcct agaaaggcgt tagattggct tacaccatta gagaaatttg
     7021 ctcagcttgt tgattatcat atggcttttg aaactgtcgc acctcatgtt tgaattcgcc
     7081 ccatattttt gctacagtga accaaattaa gatcatctat ttactaggcc tcgcatttgc
     7141 ggggttttta atgctgaata aaaggaaaac ttgatggaat tgcccaatat tatgcacccg
     7201 gtcgcgaagc tgagcaccgc attagccgct gcattgatgc tgagcgggtg catgcccggt
     7261 gaaatccgcc cgacgattgg ccagcaaatg gaaactggcg accaacggtt tggcgatctg
     7321 gttttccgcc agctcgcacc gaatgtctgg cagcacactt cctatctcga catgccgggt
     7381 ttcggggcag tcgcttccaa cggtttgatc gtcagggatg gcggccgcgt gctgttggtc
     7441 gataccgcct ggaccgatga ccagaccgcc cagatcctca actggatcaa gcaggagatc
     7501 aacctgccgg tcgcgctggc ggtggtgact cacgcgcatc aggacaagat gggcggtatg
     7561 gacgcgctgc atgcggcggg gattgcgact tatgccaatg cgttgtcgaa ccagcttgcc
     7621 ccgcaagagg ggctggttgc ggcgcaacac agcctgactt tcgccgccaa tggctgggtc
     7681 gaaccagcaa ccgcgcccaa ctttggcccg ctcaaggtat tttaccccgg ccccggccac
     7741 accagtgaca atatcaccgt tgggatcgac ggcaccgaca tcgcttttgg tggctgcctg
     7801 atcaaggaca gcaaggccaa gtcgctcggc aatctcggtg atgccgacac tgagcactac
     7861 gccgcgtcag cgcgcgcgtt tggtgcggcg ttccccaagg ccagcatgat cgtgatgagc
     7921 cattccgccc ccgatagccg cgccgcaatc actcatacgg cccgcatggc cgacaagctg
     7981 cgctgagcca tggctgacca cgtcaccccc aatctgccat cgcgcgattt cgatgtgaca
     8041 gaggcgtttt atgcgaagct gggctttgcg acgagttgga aggatcgcgg ctggatgatc
     8101 ctgcagcgcg gcggtttgca gctcgaattc ttcccctatc ctgacctcga cccagctacg
     8161 agctcgttcg gctgttgcct gcggttggat gatctcgatg ccatggtggc attggtgaac
     8221 gcggcgggag ccgaggaaaa aagcaccggc tggccgcgct tcaaagctcc gcaactggag
     8281 gcgagcggcc tgaggatcgg ctacctgatc gatcccgact gcacgctggt gcggctgatc
     8341 cagaaccccg actgaccgca tgcccgcgaa aatcaagatt tgcgggatca gcacacccga
     8401 ggcgctcgat gcgaccatcg cggcgcgggc ggactatgcc gggttggtgt tctatccagc
     8461 gtcgccccgt gcggttacgt cgaatgtcgc gggcgctttg acatcgcgcg cagctggcca
     8521 gatcgccatg gtcggtttgt tcgtcgatgc ggatgatgct gtcatcgccg acgcactggt
     8581 ggcagccaag ctgaacgcgc tgcagctgca cggttcggaa tcgcccgaac gcgtggccca
     8641 gttgcgcgcg cggtttggca agccggtgtg gaaggcgctg cccgtcgcca gcgccagcga
     8701 tgtcgcacgc gccgcagcct atgccggggc ggcggacttg atcttgttcg acgccaagac
     8761 ccccaaaggc gcgctgcccg gcggcatggg gttggcgttc gactggtcgc tgctggccgg
     8821 atatcgcggt gccttgccgt gggggctggc aggcgggcta aatccgacga atgttgccga
     8881 ggcgattgcg cgcaccggag cgccgctggt cgatacctcc agcggcgtcg aaagcgcgcc
     8941 gggcgtcaag gataccgaca agattaccaa tttcgccttt gcggtgcgct tggcctaaat
     9001 cgcgtcgatc aataggcgtc gttcagcgca aagatcggct tgcgggtgcg ccactgccct
     9061 cgggtgaagt cgggaaaatc taacgtgcga ttgccctcag caatcgattg ttccgacaga
     9121 ggcgtgatcg cgctccaggc cagcgcgtcg taaatgtcga ttggcatcgg ggccttggcc
     9181 ttcagcgcct cgacaaaagc gtggatcacg aaccagtcca tcccgccatg cccggcccct
     9241 gccgccagat cggcgtagcg tttccatagc gggtgatcgt atttcgcaaa ccagccctcg
     9301 gcaggctccc agcggtgcgg ctgtgggctc ttgccctcca gatagatcga cttgttgacg
     9361 tccatccaca gcccctcggt gccttgcacc cgaaagccga gagaataggg gcgcggcagc
     9421 gaggtgtcgt ggcacagcat gatcgtttca ccattagtgc agccgatcat ggtgttgacc
     9481 acatcaccca gtgcgaattt cacctcggcg ttgggatgat cggcagagcc gttcttgacg
     9541 acataatcat gcagcccgcg cgccttacag ccgaagccgc cagcgcccgc ttcgcccggc
     9601 aacgcgacct tcagggtgcg ggtctgcggc gggtagcaca cgccggcatc ggcgcagccc
     9661 tggtacttca cggtcagggt ggtcgcgctc gcgccggccg cgggcgtgcc ggtgagggtg
     9721 ccgagcaatt ccttgcggta ggtttcgacg tcgccgaaga attcgtcgcg gtaggccttg
     9781 cccttcggca gcgccatggt cgcgccggtg aaggcggcat cggccttgac cgaggtgcgg
     9841 tgccggtaca ggtaatagcc gtcggcgatc cgccagcgca cctcgatgcg gtccggcgcg
     9901 gtggcctgcg cggacaggac gaagacctcg tcgaccggcg gcagttcgaa gtcctgggcg
     9961 acggccgagg tcgcgggcag cgcaagcagc agggcgagcc cggccagcca gcggcgcagg
    10021 cggatcgtgg atgcggtcat tggctcagtt taccggtcgg ctctcggcgg ccagccattg
    10081 caggtattcg ggcaggccgg acgcggcttc gaccgcgagc gggtatagga agtataaacc
    10141 acctttttgc tcctcatccg aagtatctta cctgaaattc cctcactcgt ttaccgctca
    10201 agccccaatt ttaactgccg gtccagccta aaccgctcta ataaggttcg atttggcggt
    10261 aaaatctcta gcctgatagc tcgagagata caaactgccc caccgccccg tttaaaagtt
    10321 ggcagtgttg agcagtgttg gatttggggt cgtcagtcaa agagacgact ctgtgatgga
    10381 tcgaacaggc tgggagtcag tggcggcgct cgttctggtg gcagctcacg ctgcttggcg
    10441 gcattcgcct tggctgtttt ctgtttcaga tgcttgagaa tctgctcaat gacc
//
